Reference sequence
Gene mode selects an NCBI RefSeq transcript using stated rules and retains its accession, species, and selection basis in the result.
PRIMERCAT · qPCR
qPCR Primer Design
Generate qPCR primer candidates from a gene or DNA sequence, with paired genome and transcript evidence when versioned local references are available
qPCR PRIMER DESIGN
Online qPCR primer design: inputs, outputs, and evidence boundaries
PrimerCat accepts standard human or mouse gene symbols as well as custom DNA sequences. Forward and reverse primers are always reported 5′→3′, ready for ordering as shown.
Generation and screening
Primer3 generates candidates under length, Tm, GC%, amplicon, and thermodynamic constraints, followed where available by paired screening against fixed genome and transcriptome references.
How to interpret results
The candidate rank score compares pairs returned in the same run; it is not an experimental success probability. Validate efficiency, melt curves, product identity, and negative controls before use.