CrisprCat / gRNA design

CRISPR Guide Design

Scan PAM sites and rank gRNA candidates by predicted activity and off-target evidence.

DESIGN ENGINESpCas9 · SpCas9-NG · Cas12a

PAM scanning, activity ranking, and off-target screening

OUTPUT · GUIDESgRNA candidates
Awaiting target

Enter a gene or target sequence to see guides, PAMs, cut positions, and screening evidence.

All PAM sites scannedActivity-ranked resultsOff-target screening included