GHS / LCSS reagent safety

Laboratory reagent safety & hazard reference

Search common reagents by name, CAS, or formula for hazard summaries, handling controls, and incompatibilities. Each record links to its PubChem source and prompts a check of the current product SDS.

Browse reagent records ↓

If exposure or a spill occursStop work and activate the laboratory emergency procedure. For personal exposure, use the eyewash/safety shower and promptly contact onsite response, EHS, or medical services. Do not use a web summary to self-treat poisoning or manage a large spill. Follow Sections 4 and 6 of the actual product SDS.

Known incompatibilities · not exhaustive

High-risk combination alerts

Only combinations supported by authoritative sources and capable of changing waste or handling practice are shown. Absence from this list does not imply compatibility.

TRIzol / guanidine thiocyanate × hypochlorite / acid

Guanidinium lysis waste + bleach or acid

Reactive compounds and toxic gases may form. Never add bleach directly to guanidine-thiocyanate reagent or sample-preparation waste.

Immediate action

Collect separately, label clearly, and follow the product SDS and institutional hazardous-waste procedure.

Thermo Fisher · TRIzol Plus protocol ↗
Sodium hypochlorite × acid / ammonia / ammonium salt

Bleach + acid or ammonia-containing reagent

Incompatible mixing can release hazardous gases; do not use bleach on chemically unidentified waste.

Immediate action

Stop mixing and follow the laboratory gas-release/spill procedure for evacuation and reporting.

PubChem · Sodium hypochlorite LCSS ↗
Sodium azide × acid / copper / lead / silver / mercury

Sodium azide + acid or heavy-metal plumbing

Acids can generate hydrazoic acid and metals can form shock-sensitive azides; azide solutions must not be drain-disposed.

Immediate action

Use a dedicated compatible waste container and have EHS assess historical discharge or suspected deposits.

PubChem · Sodium azide LCSS ↗
Hydrogen peroxide × combustibles / reducing agents / metals

Concentrated peroxide + organic or metal contamination

Concentrated hydrogen peroxide is a strong oxidizer; contamination can accelerate decomposition, heat and gas release, ignite combustibles, or overpressurize a closed vessel.

Immediate action

Never return excess reagent to the stock bottle; isolate a contaminated container and report under the product SDS and institutional oxidizer-spill procedure.

PubChem · Hydrogen peroxide LCSS ↗
Nitric acid × alcohol / acetone / organic waste

Nitric acid + organic solvent or combustible

Nitric acid is both a strong acid and an oxidizer; contact with organics, reducing agents, or unidentified waste can cause violent heat release and toxic gases.

Immediate action

Use a dedicated compatible oxidizing-acid waste container segregated from organic waste; after accidental mixing, stop work, evacuate, and activate EHS response.

PubChem · Nitric acid LCSS ↗

Search reagents

70 results
By laboratory use
By hazard category

Acrylamide

Critical concern
CAS 79-06-1Formula C₃H₅NOType Pure substanceSignal word Danger

Unpolymerized acrylamide monomer is hazardous by ingestion, dust inhalation, and skin contact, with mutagenicity and carcinogenicity classifications.

Protein
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H301Toxic if swallowed
H340May cause genetic defects
H350May cause cancer
H372Causes organ damage through prolonged or repeated exposure

Handling controls

  • Prefer premade solutions or gels to reduce handling of monomer powder.
  • Weigh and transfer powder under risk-assessed local exhaust controls and prevent skin contact.

Critical incompatibilities

Strong oxidizers; heat or unsuitable conditions can initiate polymerization.

Boundary commonly missed

Polyacrylamide gel and unpolymerized monomer do not have the same hazard profile; do not treat them as equivalent.

Open PubChem LCSS record · CID 6579 ↗

Formaldehyde / formalin

Critical concern
CAS 50-00-0Formula CH₂OType Solution / concentration-specificSignal word Danger

Formaldehyde vapor can irritate and damage the eyes, skin, and respiratory tract; its carcinogenic and sensitizing hazards require controlled ventilation.

Cell cultureHistology
Acute toxicityCorrosiveChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H314Causes severe skin burns and eye damage
H317May cause an allergic skin reaction
H331Toxic if inhaled
H350May cause cancer

Handling controls

  • Open-container transfer and fixation work should be performed in a certified hood or institution-approved local exhaust.
  • Formalin often contains methanol; use the current formulation SDS for flammability and toxicity.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases; formulations may contain additional stabilizers.

Boundary commonly missed

Formaldehyde gas, aqueous formaldehyde, and formalin concentrations do not share one identical hazard label.

Open PubChem LCSS record · CID 712 ↗

Phenol

Critical concern
CAS 108-95-2Formula C₆H₆OType Pure substanceSignal word Danger

Phenol is corrosive and can be rapidly absorbed through skin with systemic toxicity; local numbing can mask tissue injury.

Nucleic acidProtein
Acute toxicityCorrosiveChronic hazard
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Representative GHS hazard statements

H301Toxic if swallowed
H311Toxic in contact with skin
H314Causes severe skin burns and eye damage
H341Suspected of causing genetic defects

Handling controls

  • Work in a fume hood and select gloves using permeation data in the supplier SDS; do not assume ordinary disposable gloves are sufficient.
  • Before work, confirm access to eyewash, safety shower, and the laboratory phenol-exposure procedure.

Critical incompatibilities

Strong oxidizers; avoid incompatible metals and materials listed in Section 10 of the product SDS.

Boundary commonly missed

Phenol/chloroform mixtures must be managed using the mixture SDS, not a single-ingredient record.

Open PubChem LCSS record · CID 996 ↗

Chloroform

Critical concern
CAS 67-66-3Formula CHCl₃Type Pure substanceSignal word Danger

Chloroform vapor presents acute inhalation toxicity, can damage organs after repeated exposure, and carries a suspected-carcinogen classification.

Nucleic acid
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H331Toxic if inhaled
H351Suspected of causing cancer
H372Causes organ damage through prolonged or repeated exposure

Handling controls

  • Perform all open-container work in a fume hood and close containers promptly.
  • Many common gloves have short breakthrough times for chloroform; select materials and change intervals from SDS/compatibility data.

Critical incompatibilities

Strong bases, strong oxidizers, and some reactive metals; light and air can promote hazardous decomposition, and stabilizer choice affects handling.

Boundary commonly missed

Do not use odor to judge safe exposure and do not leave chloroform open on the bench.

Open PubChem LCSS record · CID 6212 ↗

Methanol

Critical concern
CAS 67-56-1Formula CH₄OType Pure substanceSignal word Danger

Methanol is highly flammable and can cause severe poisoning through ingestion, inhalation, or skin contact, with the visual and central nervous systems among key targets.

ProteinHistologyGeneral
Acute toxicityFlammableChronic hazard
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H301Toxic if swallowed
H311Toxic in contact with skin
H370Causes damage to organs

Handling controls

  • Keep away from sparks, heat, and flames; perform large-volume or vapor-generating work in a hood.
  • Collect waste in an approved flammable-organic waste container; do not drain-dispose.

Critical incompatibilities

Strong oxidizers and ignition sources.

Boundary commonly missed

Poisoning symptoms can be delayed; any suspected exposure warrants immediate first-aid response and medical evaluation.

Open PubChem LCSS record · CID 887 ↗

Ethanol

Routine priority
CAS 64-17-5Formula C₂H₆OType Pure substanceSignal word Danger

The primary laboratory hazard of concentrated ethanol is high flammability; vapor can ignite at a remote source, and concentrated product can seriously irritate eyes.

Cell cultureGeneralCleaning
FlammableIrritant / sensitizer
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H319Causes serious eye irritation

Handling controls

  • Limit bench quantities, keep away from heat, sparks, and flames, and store as a flammable liquid.
  • Aqueous formulations such as 70% can remain flammable; dilution does not remove labeling or storage assessment.

Critical incompatibilities

Strong oxidizers and ignition sources.

Boundary commonly missed

Denatured ethanol contains additional chemicals; use that product's SDS rather than this pure-ethanol record.

Open PubChem LCSS record · CID 702 ↗

Acetic acid / glacial acetic acid

High concern
CAS 64-19-7Formula C₂H₄O₂Type Pure substanceSignal word Danger

Glacial acetic acid is corrosive and flammable, with strongly irritating vapor; dilution releases heat and can splash.

Nucleic acidProteinGeneral
CorrosiveFlammable
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Representative GHS hazard statements

H226Flammable liquid and vapor
H314Causes severe skin burns and eye damage

Handling controls

  • Open, transfer, and prepare concentrated material in a fume hood with eye/face protection matched to splash risk.
  • For dilution, slowly add acid to water with stirring and cooling, away from ignition sources.

Critical incompatibilities

Strong oxidizers, strong bases, and many reactive metals; segregate using Section 10 of the product SDS.

Boundary commonly missed

Glacial acetic acid and dilute solutions do not carry identical labels; recipe volumes must match the specified purity.

Open PubChem LCSS record · CID 176 ↗

Boric acid

High concern
CAS 10043-35-3Formula H₃BO₃Type Pure substanceSignal word Danger

Boric acid carries a reproductive-toxicity classification. Minimize dust and personal exposure when weighing powder or preparing concentrated stocks.

Nucleic acid
Chronic hazard
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Representative GHS hazard statements

H360FDMay damage fertility; may damage the unborn child

Handling controls

  • Avoid dust generation, weigh under risk-assessed local exhaust, and clean residues promptly.
  • For pregnancy, fertility planning, or occupational-exposure concerns, follow institution-specific EHS assessment and substitution requirements.

Critical incompatibilities

Do not combine outside a validated procedure; use Section 10 of the current product SDS for incompatibilities.

Boundary commonly missed

A reputation for low acute toxicity does not override its reproductive classification or institutional exposure controls.

Open PubChem LCSS record · CID 7628 ↗

Sodium dodecyl sulfate (SDS)

High concern
CAS 151-21-3Formula C₁₂H₂₅NaO₄SType Pure substanceSignal word Danger

SDS powder can irritate the respiratory tract and presents a serious eye-damage hazard; dust and splash prevention are the key controls during solution preparation.

ProteinNucleic acid
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H315Causes skin irritation
H318Causes serious eye damage
H335May cause respiratory irritation

Handling controls

  • Add powder slowly, stir gently, and use risk-assessed local exhaust with appropriate eye protection.
  • Clean powder residues promptly without dry-sweeping methods that re-aerosolize dust.

Critical incompatibilities

Strong oxidizers; confirm storage segregation in Section 10 of the current product SDS.

Boundary commonly missed

Preparing a solution reduces dust risk but does not automatically remove eye, skin, or waste-management hazards.

Open PubChem LCSS record · CID 3423265 ↗

Hydrochloric acid

High concern
CAS 7647-01-0Formula HClType Pure substanceSignal word Danger

Concentrated hydrochloric acid is strongly corrosive and releases irritating hydrogen chloride vapor; classification changes with concentration.

General
CorrosiveIrritant / sensitizer
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Representative GHS hazard statements

H290May be corrosive to metals
H314Causes severe skin burns and eye damage
H335May cause respiratory irritation

Handling controls

  • Open, transfer, and use concentrated acid for pH adjustment in a fume hood, with eye/face protection matched to splash risk.
  • For dilution, slowly add acid to water with stirring and cooling; never add water to concentrated acid.

Critical incompatibilities

Bases, reactive metals, and oxidizers; contact with cyanides or sulfides can release highly toxic gases.

Boundary commonly missed

The H290/H314/H335 summary is representative of common concentrated solutions; hydrogen chloride gas has a different classification.

Open PubChem LCSS record · CID 313 ↗

Sodium hydroxide

High concern
CAS 1310-73-2Formula NaOHType Pure substanceSignal word Danger

Solid and concentrated sodium hydroxide are strongly corrosive; dissolution and dilution release substantial heat and can cause boiling or splashing.

GeneralCleaning
Corrosive
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Representative GHS hazard statements

H290May be corrosive to metals
H314Causes severe skin burns and eye damage

Handling controls

  • Add NaOH to water slowly in small portions with continuous stirring and cooling; do not pour water directly onto a large mass of solid.
  • Use goggles, face protection, and chemically resistant PPE appropriate to concentration, scale, and splash risk.

Critical incompatibilities

Acids; metals such as aluminum and zinc can release flammable hydrogen; reacts with some organic materials.

Boundary commonly missed

Dilute solutions do not share the same label as solid or concentrated base; always use the actual product and concentration SDS.

Open PubChem LCSS record · CID 14798 ↗

Guanidine thiocyanate

Critical concern
CAS 593-84-0Formula C₂H₆N₄SType Pure substanceSignal word Danger

A common chaotrope in nucleic-acid lysis buffers; dust, concentrated solution, and splashes can be harmful by ingestion, skin contact, or inhalation.

Nucleic acid
Acute toxicityCorrosiveIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H312Harmful in contact with skin
H314Causes severe skin burns and eye damage
H332Harmful if inhaled

Handling controls

  • Use risk-assessed local exhaust for powder weighing and concentrated solutions; prevent dust and splashes.
  • Collect guanidinium-containing waste separately, label it clearly, and follow the kit or product SDS waste procedure.

Critical incompatibilities

Hypochlorite bleach and acidic solutions; never add them directly to guanidine-thiocyanate waste.

Boundary commonly missed

Guanidine thiocyanate, guanidine hydrochloride, and commercial guanidinium lysis buffers are not the same substance or formulation; check each SDS separately.

Open PubChem LCSS record · CID 65046 ↗

Guanidine hydrochloride

High concern
CAS 50-01-1Formula CH₆ClN₃Type Pure substanceSignal word Warning

Used for protein denaturation and nucleic-acid purification; powder and concentrated solutions can be harmful if swallowed and irritate skin and eyes.

Nucleic acidProtein
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H315Causes skin irritation
H319Causes serious eye irritation

Handling controls

  • Minimize dust and clean the balance and work surface promptly after weighing.
  • Assess preparation and disposal using the actual concentration, additives, and supplier SDS.

Critical incompatibilities

Strong oxidizers; use Section 10 of the product SDS for formulation-specific incompatibilities.

Boundary commonly missed

Do not mechanically transfer the bleach warning for guanidine thiocyanate to every guanidinium salt, but always check the formulation SDS for commercial lysis buffers.

Open PubChem LCSS record · CID 5742 ↗

2-Mercaptoethanol

Critical concern
CAS 60-24-2Formula C₂H₆OSType Pure substanceSignal word Danger

A volatile reducing agent that can cause severe harm by inhalation, ingestion, and skin absorption; odor is not a safe exposure indicator.

Nucleic acidProtein
Acute toxicityCorrosiveIrritant / sensitizer
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Representative GHS hazard statements

H301Toxic if swallowed
H310/H311Fatal or toxic in contact with skin
H314Causes severe skin burns and eye damage
H317May cause an allergic skin reaction

Handling controls

  • Open, pipette, and add to lysis buffers in a fume hood, using secondary containment.
  • Select gloves and change intervals from permeation data for the actual product SDS.

Critical incompatibilities

Strong oxidizers and some metals; heating can generate toxic sulfur oxides.

Boundary commonly missed

Not noticing the odor, or becoming accustomed to it, does not indicate a safe airborne concentration.

Open PubChem LCSS record · CID 1567 ↗

Dithiothreitol (DTT)

High concern
CAS 3483-12-3Formula C₄H₁₀O₂S₂Type Pure substanceSignal word Danger

A common reducing agent; powder hazards include harmful ingestion, serious eye damage, and skin or respiratory irritation.

Nucleic acidProtein
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H315Causes skin irritation
H318Causes serious eye damage
H335May cause respiratory irritation

Handling controls

  • Avoid dust and use appropriate eye protection; prefer validated premade solutions where practical.
  • Prepare small stock aliquots and label concentration, solvent, date, and storage condition.

Critical incompatibilities

Strong oxidizers; follow the product SDS for storage and reactivity details.

Boundary commonly missed

A solution reduces dust risk, but solvent and concentration can change the overall hazard classification.

Open PubChem LCSS record · CID 439196 ↗

TRIzol-type RNA extraction reagent

Critical concern
CAS —Formula MixtureType Commercial reagentSignal word Check product SDS

A commercial mixture containing phenol and guanidine thiocyanate; hazards depend on the exact formulation and cannot be represented by a single ingredient record.

Nucleic acid
Acute toxicityCorrosiveChronic hazard
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Perform opening, homogenization, phase separation, and waste transfer in a fume hood and prevent skin contact.
  • Use a separate, compatible, clearly labeled waste container for phenol/guanidinium waste.

Critical incompatibilities

Do not add bleach or acidic solutions directly to the reagent or sample-preparation waste.

Boundary commonly missed

RNA lysis reagents from different brands or versions are not one formulation; use the container label and current product SDS each time.

Thermo Fisher TRIzol product SDS ↗

Ethidium bromide (EtBr)

Critical concern
CAS 1239-45-8Formula C₂₁H₂₀BrN₃Type Pure substanceSignal word Danger

A DNA-intercalating dye whose powder inhalation hazard and suspected-mutagen classification make weighing, contamination control, and waste management critical.

Nucleic acid
Acute toxicityChronic hazard
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Representative GHS hazard statements

H302Harmful if swallowed
H330Fatal if inhaled
H341Suspected of causing genetic defects

Handling controls

  • Prefer premade solutions and use a designated area and dedicated tray to avoid handling dry powder.
  • Use an institution-approved waste stream for gels, buffers, disposables, and contaminated materials.

Critical incompatibilities

Strong oxidizers; manage solvent, storage, and disposal using the actual product SDS.

Boundary commonly missed

An alternative dye is not automatically harmless; SYBR Safe, GelRed, and similar products require their own SDS review.

Open PubChem LCSS record · CID 14710 ↗

TEMED

Critical concern
CAS 110-18-9Formula C₆H₁₆N₂Type Pure substanceSignal word Danger

A polyacrylamide polymerization accelerator that is volatile, highly flammable, corrosive, and acutely hazardous.

Protein
Acute toxicityCorrosiveFlammable
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H302Harmful if swallowed
H314Causes severe skin burns and eye damage
H332Harmful if inhaled

Handling controls

  • Pipette small quantities in a fume hood, keep away from sparks, heat, and flames, and close the container immediately.
  • Use the actual SDS to determine storage segregation and spill response for this flammable corrosive liquid.

Critical incompatibilities

Strong oxidizers, acids, and ignition sources.

Boundary commonly missed

TEMED and APS form a validated polymerization system, but each retains separate storage and disposal requirements.

Open PubChem LCSS record · CID 8037 ↗

Ammonium persulfate (APS)

Critical concern
CAS 7727-54-0Formula (NH₄)₂S₂O₈Type Pure substanceSignal word Danger

A strong oxidizer and respiratory/skin sensitizer; powder control and segregation from combustibles are central precautions.

Protein
Acute toxicityOxidizerIrritant / sensitizer
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Representative GHS hazard statements

H272May intensify fire; oxidizer
H302Harmful if swallowed
H317May cause an allergic skin reaction
H334May cause allergy or asthma symptoms if inhaled

Handling controls

  • Avoid dust with appropriate local exhaust; stop work and report any asthma or sensitization symptoms.
  • Keep the container clean and dry and prevent contamination by organics, reducing agents, or other combustibles.

Critical incompatibilities

Reducing agents, organics, and combustible materials; segregate in oxidizer storage.

Boundary commonly missed

Using fresh APS solution for polymerization does not remove its oxidizing or sensitizing hazards.

Open PubChem LCSS record · CID 62648 ↗

N,N′-Methylenebisacrylamide

Critical concern
CAS 110-26-9Formula C₇H₁₀N₂O₂Type Pure substanceSignal word Danger

A polyacrylamide gel crosslinker; the unpolymerized powder carries acute-toxicity and germ-cell-mutagenicity classifications.

Protein
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H301Toxic if swallowed
H312Harmful in contact with skin
H340May cause genetic defects

Handling controls

  • Prefer premixed acrylamide/bisacrylamide solutions to reduce dry-powder handling.
  • Designate a polymerization area, decontaminate promptly, and distinguish monomer waste from fully polymerized gels.

Critical incompatibilities

Strong oxidizers; heat or initiators can promote polymerization.

Boundary commonly missed

Fully polymerized gels differ from unpolymerized monomer, but disposal still follows institutional rules.

Open PubChem LCSS record · CID 8041 ↗

PMSF

Critical concern
CAS 329-98-6Formula C₇H₇FO₂SType Pure substanceSignal word Danger

A serine-protease inhibitor whose powder and concentrated stocks present acute toxicity and corrosive hazards.

Protein
Acute toxicityCorrosive
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Representative GHS hazard statements

H301Toxic if swallowed
H314Causes severe skin burns and eye damage

Handling controls

  • Avoid powder dust and prepare small stock aliquots with ventilation and secondary containment.
  • Also assess the flammability of common stock solvents such as isopropanol or ethanol.

Critical incompatibilities

Strong bases, strong oxidizers, and moisture; confirm in Section 10 of the actual product SDS.

Boundary commonly missed

Instability of PMSF in water does not mean degraded solutions can be treated as ordinary waste.

Open PubChem LCSS record · CID 4784 ↗

Paraformaldehyde (PFA)

Critical concern
CAS 30525-89-4Formula (CH₂O)ₙType Pure substanceSignal word Danger

A polymeric solid that releases formaldehyde; powder, heated dissolution, and concentrated solutions require ventilation and exposure controls.

Cell cultureHistology
Acute toxicityFlammableChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H228Flammable solid
H302/H332Harmful if swallowed or inhaled
H317May cause an allergic skin reaction
H351Suspected of causing cancer

Handling controls

  • Do not weigh or heat-dissolve on an open bench; use institution-approved ventilation and fixative preparation procedures.
  • Confirm formaldehyde exposure, spill, and waste procedures before work.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases; heating can release formaldehyde vapor.

Boundary commonly missed

Solid PFA, 4% PFA fixative, and formalin have different compositions and hazard labels.

Thermo Scientific paraformaldehyde product and SDS ↗

Glutaraldehyde

Critical concern
CAS 111-30-8Formula C₅H₈O₂Type Solution / concentration-specificSignal word Danger

A fixative and disinfectant whose vapor exposure can cause severe irritation, corrosion, and respiratory or skin sensitization.

Cell cultureHistologyCleaning
Acute toxicityCorrosiveIrritant / sensitizer
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Representative GHS hazard statements

H301Toxic if swallowed
H314Causes severe skin burns and eye damage
H330Fatal if inhaled
H334May cause allergy or asthma symptoms if inhaled

Handling controls

  • Use effective local exhaust or a closed system for opening, soaking, pouring, and waste transfer.
  • Stop work and follow occupational-health reporting for wheeze, chest tightness, or rash.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases; manage different concentrations and activated formulations by product SDS.

Boundary commonly missed

A diluted disinfectant formulation is not automatically free of vapor or sensitization risk.

Open PubChem LCSS record · CID 3485 ↗

Trypan blue

Critical concern
CAS 72-57-1Formula C₃₄H₂₄N₆Na₄O₁₄S₄Type Solution / concentration-specificSignal word Danger

A common viability dye; the example commercial stain SDS classifies it for carcinogenic and reproductive hazards, so each concentration and formulation must be checked.

Cell culture
Chronic hazard
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Representative GHS hazard statements

H350May cause cancer
H361Suspected of damaging fertility or the unborn child

Handling controls

  • Avoid aerosols and skin contact, use a closed waste container, and control pipetting contamination.
  • Treat powder, 0.4% stain, and other commercial concentrations according to their own SDS.

Critical incompatibilities

Strong oxidizers; dispose under the product SDS and institutional dye-waste procedure.

Boundary commonly missed

Do not treat it as an ordinary aqueous solution merely because the working volume is small or it is used for cell counting.

Thermo Fisher Trypan Blue Stain SDS · CID 6296 ↗

DAPI dihydrochloride

Routine priority
CAS 28718-90-3Formula C₁₆H₁₇Cl₂N₅Type Pure substanceSignal word No signal word

The example DAPI dihydrochloride SDS does not classify the product as GHS hazardous, while noting that its toxicological properties have not been thoroughly investigated.

Cell cultureHistology
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Continue prudent laboratory handling and avoid powder, skin contact, and unnecessary aerosols.
  • Prepared solutions, mounting media, and kits may contain different solvents or additives and require their own SDS.

Critical incompatibilities

The example SDS reports no known hazardous reaction; use Section 10 of the actual SDS for other formulations.

Boundary commonly missed

Not classified as hazardous is not proof of no toxicity and does not replace prudent control of unknown hazards.

Thermo Fisher DAPI dihydrochloride SDS ↗

Hoechst 33342

Critical concern
CAS 23491-52-3Formula C₂₇H₂₈N₆OType Pure substanceSignal word Warning

A cell-permeant DNA-binding dye with representative classifications for irritation and suspected germ-cell mutagenicity.

Cell cultureHistology
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H315Causes skin irritation
H335May cause respiratory irritation
H341Suspected of causing genetic defects

Handling controls

  • Prefer low-volume premade solutions, avoid powder weighing, and control staining waste.
  • Suitability for live-cell experiments does not mean the dye is harmless to the operator.

Critical incompatibilities

Strong oxidizers; use the actual product SDS for solvent and salt form.

Boundary commonly missed

Hoechst 33342 powder, DMSO stock, and ready-to-use products can carry different hazard labels.

Open PubChem LCSS record · CID 1464 ↗

Isopropanol

Routine priority
CAS 67-63-0Formula C₃H₈OType Pure substanceSignal word Danger

A common solvent for nucleic-acid precipitation and disinfection; vapor accumulation, remote ignition sources, and confined spaces are key risks.

Nucleic acidCell cultureGeneralCleaning
FlammableIrritant / sensitizer
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H319Causes serious eye irritation
H336May cause drowsiness or dizziness

Handling controls

  • Limit bench quantities and keep away from sparks, heat, and flames; ensure effective ventilation for larger use.
  • Store and collect as a flammable liquid; do not use an ordinary refrigerator for bulk flammable solvent.

Critical incompatibilities

Strong oxidizers and ignition sources.

Boundary commonly missed

70% isopropanol can remain flammable; disinfectant use does not remove flammable-liquid controls.

Open PubChem LCSS record · CID 3776 ↗

Acetone

Routine priority
CAS 67-64-1Formula C₃H₆OType Pure substanceSignal word Danger

A fast-evaporating, highly flammable solvent whose bench cleaning and fixation use can lead to underestimated vapor and ignition risks.

GeneralHistologyCleaning
FlammableIrritant / sensitizer
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H319Causes serious eye irritation
H336May cause drowsiness or dizziness

Handling controls

  • Dispense only the needed quantity, close promptly, keep away from ignition sources, and maintain ventilation.
  • Place solvent wipes and waste liquid in institution-designated flammable-solvent waste containers.

Critical incompatibilities

Strong oxidizers, strong acids, strong bases, and ignition sources.

Boundary commonly missed

Rapid evaporation increases airborne vapor and does not make residues automatically safe.

Open PubChem LCSS record · CID 180 ↗

Acetonitrile

Critical concern
CAS 75-05-8Formula C₂H₃NType Pure substanceSignal word Danger

A common HPLC/MS and peptide-processing solvent that is highly flammable and acutely harmful by inhalation, skin contact, and ingestion.

GeneralProteinNucleic acid
Acute toxicityFlammableIrritant / sensitizer
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Representative GHS hazard statements

H225Highly flammable liquid and vapor
H302Harmful if swallowed
H312Harmful in contact with skin
H332Harmful if inhaled

Handling controls

  • Maintain ventilation during mobile-phase preparation and instrument waste collection; verify waste-bottle closure and fill level.
  • Tubing, fittings, and waste containers must be acetonitrile-compatible and kept from ignition sources.

Critical incompatibilities

Strong oxidizers, strong acids, strong bases, and ignition sources.

Boundary commonly missed

HPLC waste is a mixture; classification and disposal must account for all mobile-phase and sample components.

Open PubChem LCSS record · CID 6342 ↗

N,N-Dimethylformamide (DMF)

Critical concern
CAS 68-12-2Formula C₃H₇NOType Pure substanceSignal word Danger

A highly penetrating solvent with dermal and inhalation exposure hazards and a reproductive-toxicity classification.

Cell cultureGeneral
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H312Harmful in contact with skin
H319Causes serious eye irritation
H332Harmful if inhaled
H360DMay damage the unborn child

Handling controls

  • Use a fume hood and prevent skin contact; select gloves using DMF-specific permeation data.
  • Follow institutional designated-area, training, and personnel-protection requirements for reproductive toxins.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases; hazardous reactions can occur with some halogenating agents.

Boundary commonly missed

DMF can permeate many common gloves; wearing gloves alone does not demonstrate effective protection.

Open PubChem LCSS record · CID 6228 ↗

Sodium hypochlorite / bleach

Critical concern
CAS 7681-52-9Formula NaOClType Solution / concentration-specificSignal word Danger

A common disinfectant and oxidizer whose corrosivity depends on concentration, pH, and formulation; incompatible mixing can rapidly release toxic gases.

Cell cultureCleaningGeneral
CorrosiveOxidizerIrritant / sensitizer
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Representative GHS hazard statements

H290May be corrosive to metals
H314Causes severe skin burns and eye damage
H400Very toxic to aquatic life

Handling controls

  • Dilute only under a validated SOP with ventilation and label the active concentration and preparation date.
  • Before use, confirm the surface or waste contains no acid, ammonia, or guanidinium lysis reagent.

Critical incompatibilities

Acids, ammonia/ammonium salts, guanidine thiocyanate, reducing agents, and many organics.

Boundary commonly missed

Household bleach, laboratory hypochlorite, and different available-chlorine concentrations do not share one label and degrade during storage.

Open PubChem LCSS record · CID 23665760 ↗

Sodium azide

Critical concern
CAS 26628-22-8Formula NaN₃Type Pure substanceSignal word Danger

Used as a preservative in antibody and buffer solutions; it is highly acutely toxic and can create additional hazards with acids or heavy metals.

ProteinCell cultureGeneral
Acute toxicityChronic hazard
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Representative GHS hazard statements

H300Fatal if swallowed
H400Very toxic to aquatic life
H410Very toxic to aquatic life with long lasting effects

Handling controls

  • Use the smallest practical amount, prevent dust and skin contact, and maintain a clearly designated azide waste stream.
  • Do not drain-dispose and assess plumbing for copper, lead, silver, and similar metals.

Critical incompatibilities

Acids can generate toxic, explosive hydrazoic acid; copper, lead, silver, mercury, and similar metals can form shock-sensitive metal azides.

Boundary commonly missed

Low-concentration preservative waste can still accumulate in metal drain systems and must not be treated as ordinary buffer waste.

Open PubChem LCSS record · CID 33557 ↗

Sodium chloride

Routine priority
CAS 7647-14-5Formula NaClType Pure substanceSignal word Classification varies

A common buffer and culture-medium salt; PubChem aggregates classifications ranging from not classified or low concern to eye-damage and aquatic-hazard classifications, so the current product SDS governs.

Nucleic acidProteinCell cultureGeneral
Irritant / sensitizerChronic hazard
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Representative GHS hazard statements

H318Causes serious eye damage
H412Harmful to aquatic life with long lasting effects

Handling controls

  • Minimize dust and prevent eye exposure when weighing bulk powder.
  • Label concentrated salt solutions; their osmotic and equipment-corrosion effects must not be treated like plain water.

Critical incompatibilities

Strong oxidizers; saline mixtures containing other reagents or biological samples require whole-mixture reassessment.

Boundary commonly missed

Routine saline or PBS does not share the same risk profile as bulk dry powder, concentrated solution, or contaminated high-salt waste; GHS classifications differ among sources.

Open PubChem LCSS record · CID 5234 ↗

Potassium chloride

Routine priority
CAS 7447-40-7Formula KClType Pure substanceSignal word No uniform signal word

A common buffer, electrolyte, and culture-medium component; most classifications aggregated by PubChem do not classify it as GHS hazardous, while dust and concentrated solutions still require routine controls.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust, ingestion, and eye contact, and clean the work surface after weighing.
  • Label stock concentration and intended use to prevent it being mistaken for a ready-to-use isotonic solution.

Critical incompatibilities

Strong oxidizers; mixture compatibility depends on the other components.

Boundary commonly missed

Low intrinsic hazard does not remove the biological effects of concentrated electrolyte or cover additives and biological contaminants.

Open PubChem LCSS record · CID 4873 ↗

Disodium phosphate (anhydrous)

Routine priority
CAS 7558-79-4Formula Na₂HPO₄Type Pure substanceSignal word Warning (representative classification)

A common phosphate-buffer component; most sources aggregated by PubChem do not classify it, while some report a serious-eye-irritation classification.

Nucleic acidProteinCell cultureGeneral
Irritant / sensitizer
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Representative GHS hazard statements

H319Causes serious eye irritation

Handling controls

  • Control dust and wear eye protection during weighing.
  • Confirm anhydrous versus the exact hydrate first; molecular mass and required mass differ by form.

Critical incompatibilities

Strong acids and strong oxidizers; phosphate can precipitate with multivalent metal ions.

Boundary commonly missed

CAS 7558-79-4 is the anhydrous form; mono-, di-, and heptahydrates cannot use the same mass directly, and supplier classifications can differ.

Open PubChem LCSS record · CID 24203 ↗

Monopotassium phosphate

Routine priority
CAS 7778-77-0Formula KH₂PO₄Type Pure substanceSignal word No uniform signal word

A common phosphate-buffer and culture-medium component; most sources aggregated by PubChem do not classify it as GHS hazardous.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust, ingestion, and eye exposure, and keep the container dry and closed after weighing.
  • Clearly label concentrated stocks with concentration, pH, and preparation date.

Critical incompatibilities

Strong bases and strong oxidizers; phosphate can precipitate with multivalent metal ions.

Boundary commonly missed

Absence of classification by most sources does not permit drain disposal of phosphate waste containing samples, heavy metals, or other hazardous reagents.

Open PubChem LCSS record · CID 516951 ↗

Trisodium citrate dihydrate

Routine priority
CAS 6132-04-3Formula C₆H₅Na₃O₇·2H₂OType Pure substanceSignal word No uniform signal word

Used in SSC, buffers, and anticoagulant systems; the large majority of sources aggregated by PubChem do not classify the dihydrate as GHS hazardous.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust and eye contact during weighing and confirm that the label specifies the dihydrate.
  • Record concentration, pH, and microbial-control method for stock solutions.

Critical incompatibilities

Strong oxidizers; citrate can complex metal ions and change the behavior of metal-containing systems or waste.

Boundary commonly missed

The dihydrate and anhydrous forms require different masses; citrate waste containing metals or biological samples cannot be assessed as a low-hazard salt solution alone.

Open PubChem LCSS record · CID 71474 ↗

Sodium carbonate (anhydrous)

Routine priority
CAS 497-19-8Formula Na₂CO₃Type Pure substanceSignal word Warning

An alkaline salt used in ELISA coating buffers and pH adjustment; serious eye irritation is the primary concern for powder and concentrated solution.

ProteinCell cultureGeneralCleaning
Irritant / sensitizer
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Representative GHS hazard statements

H319Causes serious eye irritation

Handling controls

  • Minimize dust and use eye protection during weighing; follow the product SDS immediately after eye exposure.
  • Label concentrated solutions for alkalinity and concentration and add slowly during pH adjustment.

Critical incompatibilities

Acids release carbon dioxide and can overpressurize closed containers; keep away from strong acids and incompatible metals.

Boundary commonly missed

Anhydrous sodium carbonate and its mono- and decahydrates have different molecular masses and cannot share one recipe mass.

Open PubChem LCSS record · CID 10340 ↗

Sodium bicarbonate

Routine priority
CAS 144-55-8Formula NaHCO₃Type Pure substanceSignal word No uniform signal word

A common component of media, carbonate buffers, and pH control; it generally has no uniform GHS hazard classification, while dust and gas evolution during acidification still require control.

ProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust and eye exposure and keep the container dry and closed.
  • For cell-culture formulations, verify sterility, endotoxin, and incubator CO₂ conditions.

Critical incompatibilities

Acids release CO₂; do not rapidly combine with acid in a closed vessel, and heating can also cause gas-releasing decomposition.

Boundary commonly missed

Low intrinsic hazard does not make every bicarbonate-containing medium drain-disposable; antibiotics, cells, and other additives determine the complete waste classification.

Open PubChem LCSS record · CID 516892 ↗

Bovine serum albumin (BSA)

Routine priority
CAS 9048-46-8Formula Biological proteinType Commercial reagentSignal word No signal word (cited product)

A common blocker, stabilizer, and protein standard; the cited product SDS classifies it as not hazardous, but it remains animal-derived biological material and a dust-forming protein powder.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Prevent protein dust during weighing, use eye and hand protection, and clean residues promptly.
  • Select low-endotoxin, fatty-acid-free, IgG-free, or protease-free grades as required and record the lot.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases can denature the protein; mixture hazards depend on preservatives and other additives.

Boundary commonly missed

BSA is a complex biological product rather than a single small molecule; one product SDS does not replace SDSs for every source, grade, and formulation.

MilliporeSigma · Bovine serum albumin SDS ↗

Ampicillin sodium

High concern
CAS 69-52-3Formula C₁₆H₁₈N₃NaO₄SType Pure substanceSignal word Danger

A beta-lactam selection antibiotic whose dust can sensitize skin and airways; repeated small exposures must not be treated as ordinary low-hazard powder.

Cell cultureGeneral
Irritant / sensitizer
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Representative GHS hazard statements

H315Causes skin irritation
H317May cause an allergic skin reaction
H319Causes serious eye irritation
H334May cause allergy or asthma symptoms or breathing difficulties if inhaled

Handling controls

  • Prevent dust inhalation and skin contact, weigh under risk-assessed local exhaust, and use a dedicated cleanup procedure.
  • Work arrangements for people with known penicillin or beta-lactam allergy follow institutional occupational-health requirements.

Critical incompatibilities

Strong oxidizers; heat, repeated freeze-thaw cycles, and unsuitable pH reduce potency, with product instructions governing stability.

Boundary commonly missed

Anhydrous material, sodium salt, and products with different potency or hydration cannot be interchanged by abbreviation; biological activity in media is also part of the waste boundary.

Thermo Fisher · Ampicillin sodium SDS · CID 23663979 ↗

Kanamycin A sulfate

Critical concern
CAS 25389-94-0Formula C₁₈H₃₈N₄O₁₅SType Pure substanceSignal word Danger

A biologically active aminoglycoside antibiotic; PubChem aggregates reproductive-toxicity, repeated-exposure organ-damage, and aquatic-hazard classifications.

Cell cultureGeneral
Chronic hazard
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Representative GHS hazard statements

H360May damage fertility or the unborn child
H372Causes damage to organs through prolonged or repeated exposure
H400Very toxic to aquatic life
H410Very toxic to aquatic life with long lasting effects

Handling controls

  • Weigh under institutional designated-area and training controls for reproductive toxins while preventing dust, skin contact, and cross-contamination.
  • Clearly label stocks and cultures with antibiotic identity and concentration and use a dedicated waste stream.

Critical incompatibilities

Strong oxidizers; the specific product instructions govern mixture stability and potency.

Boundary commonly missed

The listed PubChem CAS corresponds to kanamycin A 1:1 sulfate; products with different sulfate stoichiometry or potency cannot be interchanged by mass.

Open PubChem LCSS record · CID 441374 ↗

Chloramphenicol

Critical concern
CAS 56-75-7Formula C₁₁H₁₂Cl₂N₂O₅Type Pure substanceSignal word Danger

An active bacterial-selection antibiotic; the cited supplier SDS classifies it as a Category 1B carcinogen, requiring carcinogen controls for solid handling and waste.

Cell cultureGeneral
Chronic hazard
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Representative GHS hazard statements

H350May cause cancer

Handling controls

  • Allow trained personnel only to weigh it in a designated area under assessed local exhaust while preventing dust and skin contact.
  • Use dedicated tools and waste containers and decontaminate and document work under the carcinogen procedure.

Critical incompatibilities

Strong oxidizers; ethanol stocks must also be kept from heat, sparks, and flames.

Boundary commonly missed

Small antibiotic volumes do not remove the carcinogenicity classification; cultures, ethanol, and other additives require complete-mixture and strictest-waste assessment.

Thermo Fisher · Chloramphenicol SDS · CID 5959 ↗

Tris base

Routine priority
CAS 77-86-1Formula C₄H₁₁NO₃Type Pure substanceSignal word Warning

A common biological buffer; eye, skin, and respiratory irritation are the primary concerns during powder weighing and concentrated-stock preparation.

Nucleic acidProteinGeneral
Irritant / sensitizer
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Representative GHS hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation

Handling controls

  • Minimize dust, weigh under local exhaust, and use suitable eye and hand protection.
  • Add acid or base slowly during pH adjustment and control the temperature rise.

Critical incompatibilities

Strong oxidizers; separately assess strong acids or bases used for pH adjustment.

Boundary commonly missed

Dilute Tris buffer does not have the same hazard profile as solid powder or concentrated stock.

Open PubChem LCSS record · CID 6503 ↗

Disodium EDTA dihydrate

High concern
CAS 6381-92-6Formula C₁₀H₁₈N₂Na₂O₁₀Type Commercial reagentSignal word Warning

A common chelator; the cited dihydrate SDS classifies dust or mist inhalation as harmful and warns that repeated inhalation may damage the respiratory system.

Nucleic acidProteinCell cultureGeneral
Acute toxicityChronic hazard
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Representative GHS hazard statements

H332Harmful if inhaled
H373May cause damage to organs through prolonged or repeated exposure

Handling controls

  • Use the container CAS and hydration state to retrieve the matching SDS and prevent dust during weighing.
  • Control pH adjustment during concentrated-stock preparation and label the salt form and concentration.

Critical incompatibilities

Strong oxidizers; it can chelate many metal ions and change the properties of metal-containing waste.

Boundary commonly missed

EDTA, anhydrous disodium EDTA, and the dihydrate are not one product identity; CAS 6381-92-6 here matches the dihydrate formula.

Thermo Fisher · Disodium EDTA dihydrate SDS · CID 44120005 ↗

Urea

Routine priority
CAS 57-13-6Formula CH₄N₂OType Pure substanceSignal word No uniform signal word

A common protein denaturant and nucleic-acid gel component; representative sources do not provide one GHS classification for every product, but dust and concentrated solutions still require good control.

Nucleic acidProteinGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Minimize dust during weighing, prevent eye contact, and keep the container dry and closed.
  • Do not strongly heat urea solutions for prolonged periods; control temperature and freshness under the protocol.

Critical incompatibilities

Strong oxidizers and nitrites; high-temperature decomposition can produce irritating products.

Boundary commonly missed

Not classified as hazardous under a given GHS source does not mean dust, thermal decomposition, or other sample components can be ignored.

Open PubChem LCSS record · CID 1176 ↗

Imidazole

Critical concern
CAS 288-32-4Formula C₃H₄N₂Type Pure substanceSignal word Danger

A common Ni-NTA protein-purification eluent; the solid and concentrated solutions require attention for corrosivity and reproductive toxicity and should not be treated as an ordinary buffer salt.

ProteinGeneral
Acute toxicityCorrosiveChronic hazard
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Representative GHS hazard statements

H302Harmful if swallowed
H314Causes severe skin burns and eye damage
H360DMay damage the unborn child

Handling controls

  • Use local exhaust for weighing and concentrated-stock preparation and prevent dust, splashes, and skin contact.
  • Follow institutional designated-area and personnel-protection requirements for reproductive toxins.

Critical incompatibilities

Strong acids and strong oxidizers.

Boundary commonly missed

A dilute elution buffer may be classified differently from pure imidazole powder, but the actual formulation SDS and risk assessment still govern.

Open PubChem LCSS record · CID 795 ↗

Glycine

Routine priority
CAS 56-40-6Formula C₂H₅NO₂Type Pure substanceSignal word No uniform signal word

A common electrophoresis and transfer-buffer component; it generally has no uniform hazard classification, but powder weighing still requires dust control.

ProteinGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust and eye contact and use clean tools to prevent cross-contamination.
  • Assess the complete buffer for other components such as methanol or SDS.

Critical incompatibilities

Strong oxidizers; incompatibilities of mixed buffers depend on their other components.

Boundary commonly missed

Glycine's low intrinsic hazard does not make methanol-containing transfer buffer or SDS-containing running buffer equally low hazard.

Open PubChem LCSS record · CID 750 ↗

Glycerol

Routine priority
CAS 56-81-5Formula C₃H₈O₃Type Pure substanceSignal word No uniform signal word

Common in cryopreservation, loading buffers, and enzyme formulations; it usually has no uniform GHS hazard classification, but viscous spills create slip hazards and overheating can produce irritating fumes.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Clean drips promptly and keep floors and benches residue-free.
  • Check formulation limits before heat sterilization or high-temperature work and avoid localized overheating.

Critical incompatibilities

Strong oxidizers; keep away from uncontrolled heat and ignition sources.

Boundary commonly missed

The main hazard in a glycerol-containing cryostock may come from DMSO, biological material, or other additives.

Open PubChem LCSS record · CID 753 ↗

Sucrose

Routine priority
CAS 57-50-1Formula C₁₂H₂₂O₁₁Type Pure substanceSignal word No uniform signal word

Used in density gradients, stabilizers, and media; it generally has no uniform hazard classification, but large quantities of fine dust require combustible-dust controls.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Avoid dust during routine small-scale weighing; assess dust accumulation and ignition sources for scale-up or bulk handling.
  • Concentrated solutions can foul surfaces and support microbial growth; clean promptly and label storage conditions.

Critical incompatibilities

Strong oxidizers; keep dust clouds away from ignition sources.

Boundary commonly missed

Gram-scale laboratory work and industrial bulk dust differ in scale, but neither should generate a visible dust cloud.

Open PubChem LCSS record · CID 5988 ↗

Tween 20 / polysorbate 20

Routine priority
CAS 9005-64-5Formula MixtureType Commercial reagentSignal word No signal word (cited product)

A common nonionic surfactant; the cited product SDS classifies the supplied product as not hazardous, but grades, additives, and contaminants require separate review.

ProteinCell cultureGeneralCleaning
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Prevent eye contact and promptly remove slippery drips to avoid falls and cross-contamination.
  • For wash buffers, assess preservatives, azides, and other additives in the complete formulation.

Critical incompatibilities

Strong oxidizers; consult Section 10 of the specific product SDS.

Boundary commonly missed

Tween 20 is a composition-distribution commercial material; do not invent one fixed molecular formula or apply one brand SDS to every product.

MilliporeSigma · Tween 20 SDS ↗

Triton X-100

High concern
CAS 9002-93-1Formula DistributionType MixtureSignal word Danger

A common nonionic detergent; skin and eye irritation and aquatic environmental hazards require control during pipetting, cleanup, and waste handling.

Nucleic acidProteinCell cultureGeneralCleaning
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H315Causes skin irritation
H318Causes serious eye damage
H410Very toxic to aquatic life with long lasting effects

Handling controls

  • Use eye and hand protection, clean drips immediately, and separately assess the disposal route for Triton-containing waste.
  • Do not equate nonionic detergent with harmless or drain-disposable.

Critical incompatibilities

Strong oxidizers; follow the stability and reactivity section of the current product SDS.

Boundary commonly missed

Triton X-100 is an ethoxylate distribution rather than one molecule; database structures are representative search aids only.

MilliporeSigma · Triton X-100 SDS · CID 5590 ↗

Ammonium sulfate

Routine priority
CAS 7783-20-2Formula (NH₄)₂SO₄Type Pure substanceSignal word Warning (classification varies)

A salt used in protein precipitation and media; aggregated classifications vary, while dust inhalation, eye irritation, and salt loading in bulk waste require attention.

ProteinGeneral
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H319Causes serious eye irritation

Handling controls

  • Control dust during weighing and confirm the grade-specific classification in the current supplier SDS.
  • Assess large-volume high-salt waste under local discharge and hazardous-waste rules rather than assuming drain disposal.

Critical incompatibilities

Strong oxidizers and strong bases; strong bases can release ammonia.

Boundary commonly missed

Aggregated database H-codes may not match the product in hand, so this record explicitly marks classification as product-dependent.

Open PubChem LCSS record · CID 6097028 ↗

Ammonium chloride

High concern
CAS 12125-02-9Formula NH₄ClType Pure substanceSignal word Warning

A salt used in red-cell lysis and buffer systems; it is harmful if swallowed and causes serious eye irritation, while dust and thermal decomposition products also require control.

ProteinCell cultureGeneral
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H319Causes serious eye irritation

Handling controls

  • Prevent dust and use eye protection during weighing and dissolution.
  • Before heating or combining with strong base, assess ammonia release and maintain effective ventilation.

Critical incompatibilities

Strong bases can release ammonia; strong oxidizers and some halogen compounds.

Boundary commonly missed

Dilute red-cell lysis buffer may carry a different label from solid ammonium chloride, and mixture risk depends on every component.

Open PubChem LCSS record · CID 25517 ↗

Dimethyl sulfoxide (DMSO)

High concern
CAS 67-68-5Formula C₂H₆OSType Pure substanceSignal word Warning (classification varies)

Common in cryopreservation and compound stocks; classifications of the neat solvent vary, while a key practical concern is its ability to facilitate dermal exposure to dissolved compounds.

Cell cultureGeneral
Irritant / sensitizer
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Representative GHS hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation

Handling controls

  • Manage DMSO stocks according to the most hazardous solute and select gloves for both solvent and solute.
  • Prevent skin contact during cryomedium preparation; clinical or cell-therapy use requires separate dedicated standards.

Critical incompatibilities

Strong oxidizers, strong acids, strong bases, and some acyl-halide or halogenating reagents; reactions and scale-up require dedicated assessment.

Boundary commonly missed

The label for neat DMSO does not represent drugs, dyes, or toxic compounds dissolved in it; manage the mixture for every component.

Open PubChem LCSS record · CID 679 ↗

Dichloromethane

Critical concern
CAS 75-09-2Formula CH₂Cl₂Type Pure substanceSignal word Warning

A highly volatile halogenated solvent whose vapor can affect the central nervous system and carries a carcinogenicity concern.

HistologyGeneral
Acute toxicityChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H336May cause drowsiness or dizziness
H351Suspected of causing cancer

Handling controls

  • Perform all open-container, transfer, and waste operations in a certified fume hood and close containers immediately.
  • Select gloves using dichloromethane permeation data; many common disposable gloves provide very short protection.

Critical incompatibilities

Strong oxidizers, strong bases, reactive metals, and high heat; avoid incompatible plastics.

Boundary commonly missed

Odor is not a safe exposure indicator, and limited flammability does not make open-bench use acceptable.

Open PubChem LCSS record · CID 6344 ↗

Xylene, mixed isomers

High concern
CAS 1330-20-7Formula C₈H₁₀Type MixtureSignal word Danger (typical product classification)

A flammable mixture used in tissue clearing and solvent work; vapor can irritate the eyes, nose, and throat and cause headache, dizziness, and impaired coordination.

HistologyGeneralCleaning
Acute toxicityFlammableChronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H226Flammable liquid and vapor
H304May be fatal if swallowed and enters airways
H315Causes skin irritation
H332Harmful if inhaled

Handling controls

  • Use a fume hood or validated tissue processor, control vapor, and keep away from ignition sources.
  • Check the supplier SDS for the actual isomer ratio and additives and use a compatible waste container.

Critical incompatibilities

Strong oxidizers, strong acids, and ignition sources.

Boundary commonly missed

CAS 1330-20-7 denotes an isomer mixture; data for pure o-, m-, or p-xylene do not fully replace the current mixture SDS.

CDC/NIOSH · Xylene ↗

Hydrogen peroxide

Critical concern
CAS 7722-84-1Formula H₂O₂Type Solution / concentration-specificSignal word Danger (concentrate)

Oxidizing and corrosive hazards are strongly concentration-dependent; dilute disinfectant, 30% laboratory solution, and higher concentrations do not share one hazard label.

Cell cultureHistologyGeneralCleaning
Acute toxicityCorrosiveOxidizer
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Representative GHS hazard statements

H271May cause fire or explosion; strong oxidizer
H302Harmful if swallowed
H314Causes severe skin burns and eye damage
H332Harmful if inhaled

Handling controls

  • First verify concentration and stabilizers; handle concentrates with ventilation, chemical-splash protection, and compatible secondary containment.
  • Maintain cleanliness and prevent organic or metal contamination and pressure buildup from decomposition gas in closed systems.

Critical incompatibilities

Combustible or organic materials, reducing agents, many metals and metal salts, strong bases, and contaminants.

Boundary commonly missed

The displayed H-codes provide conservative hazard recognition for concentrate; the SDS for the actual weight percentage governs.

Open PubChem LCSS record · CID 784 ↗

Sulfuric acid

Critical concern
CAS 7664-93-9Formula H₂SO₄Type Solution / concentration-specificSignal word Danger

A strongly corrosive acid whose dilution is highly exothermic; concentrated acid can dehydrate and char organics with splash hazards. Acid-mist exposure requires separate control.

General
CorrosiveAcute toxicityChronic hazard
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Representative GHS hazard statements

H290May be corrosive to metals
H314Causes severe skin burns and eye damage

Handling controls

  • Add acid slowly to water with stirring and cooling; never pour water into concentrated sulfuric acid.
  • Use a fume hood, acid-resistant eye/face protection, SDS-verified gloves/apron, and compatible secondary containment.

Critical incompatibilities

Water during dilution (violent heat release), bases, reactive metals, reducing agents, and combustible or organic materials.

Boundary commonly missed

Dilute sulfuric acid can still seriously injure eyes; lower concentration does not replace review of the actual solution SDS and mixing heat.

Open PubChem LCSS record · CID 1118 ↗

Nitric acid

Critical concern
CAS 7697-37-2Formula HNO₃Type Solution / concentration-specificSignal word Danger

A strongly corrosive oxidizing acid that can release toxic nitrogen oxides; contact with organics or reducing agents can be violent.

GeneralCleaning
Acute toxicityCorrosiveOxidizer
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Representative GHS hazard statements

H272May intensify fire; oxidizer
H314Causes severe skin burns and eye damage
H331Toxic if inhaled

Handling controls

  • Handle small quantities in a hood suitable for strong oxidizing acids with acid-resistant splash protection and compatible secondary containment.
  • Segregate from organic acids, solvents, and ordinary combustibles; add acid slowly to water with cooling during dilution.

Critical incompatibilities

Organics, combustibles, reducing agents, bases, reactive metals, and many solvents.

Boundary commonly missed

Fuming nitric acid, concentrated nitric acid, and dilute solutions have different classifications and storage requirements and must be managed by actual concentration.

Open PubChem LCSS record · CID 944 ↗

Coomassie Brilliant Blue R-250

Routine priority
CAS 6104-59-2Formula C₄₅H₄₄N₃NaO₇S₂Type Commercial reagentSignal word Warning (classification varies)

A protein-gel stain whose powder can create contamination and exposure; major added hazards in classic staining solutions often come from methanol and acetic acid.

Protein
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H319Causes serious eye irritation

Handling controls

  • Control dust and use dedicated tools for powder; ventilate staining and destaining according to the full formulation.
  • Classify staining waste by both dye and solvents rather than as ordinary aqueous waste.

Critical incompatibilities

Strong oxidizers; methanol-containing formulations must also be kept from ignition sources.

Boundary commonly missed

R-250, G-250, and ready-to-use methanol-free formulations are not the same material; verify the dye form and solvents.

Open PubChem LCSS record · CID 61365 ↗

Propidium iodide (PI)

High concern
CAS 25535-16-4Formula C₂₇H₃₄I₂N₄Type Commercial reagentSignal word Warning

A nucleic-acid intercalating fluorescent dye; control powder, concentrated stock, staining waste, and contaminated disposables as a potential genotoxic compound.

Cell cultureNucleic acid
Chronic hazardIrritant / sensitizer
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Representative GHS hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
H341Suspected of causing genetic defects

Handling controls

  • Prefer sealed small packs or ready-to-use solution and work in a designated area while preventing splashes and aerosols.
  • Maintain clearly labeled PI liquid and solid waste streams and decontaminate or dispose under institutional rules.

Critical incompatibilities

Strong oxidizers; consult the product SDS for solvent and stabilizers.

Boundary commonly missed

Micromolar working concentration does not eliminate cumulative contamination concerns, and PI should not be assumed equivalent to other nucleic-acid stains.

Open PubChem LCSS record · CID 104981 ↗

Cycloheximide (CHX)

Critical concern
CAS 66-81-9Formula C₁₅H₂₃NO₄Type Pure substanceSignal word Danger

A eukaryotic protein-synthesis inhibitor with extreme acute oral toxicity and mutagenic and reproductive-toxicity concerns.

Cell cultureProtein
Acute toxicityChronic hazard
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Representative GHS hazard statements

H300Fatal if swallowed
H341Suspected of causing genetic defects
H360DMay damage the unborn child
H411Toxic to aquatic life with long lasting effects

Handling controls

  • Prefer premade solution; weigh powder and prepare stocks in a designated area using assessed containment or local exhaust.
  • Use the smallest amount and establish separate liquid, solid, and contaminated-equipment waste streams.

Critical incompatibilities

Strong oxidizers; manage the actual solvent formulation under its current SDS.

Boundary commonly missed

Low final concentration in culture medium does not remove the high-hazard controls required for stock and contaminated waste.

Open PubChem LCSS record · CID 6197 ↗

Osmium tetroxide

Critical concern
CAS 20816-12-0Formula OsO₄Type Solution / concentration-specificSignal word Danger

A highly volatile, acutely toxic oxidizer used in electron-microscopy fixation; very small vapor exposures can severely injure the eyes and respiratory system.

Histology
Acute toxicityCorrosiveChronic hazard
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Representative GHS hazard statements

H300Fatal if swallowed
H310Fatal in contact with skin
H314Causes severe skin burns and eye damage
H330Fatal if inhaled

Handling controls

  • Use only in an institution-approved dedicated hood under a written SOP with closed secondary containment, minimum quantities, and a prepared spill-response plan.
  • Before work, confirm suitable detection or sorbent materials, exposure response, and waste recovery; untrained personnel must not attempt spill cleanup.

Critical incompatibilities

Organics, reducing agents, combustible materials, and many metals; contaminated materials can continue releasing vapor.

Boundary commonly missed

Aqueous solutions still release osmium tetroxide vapor; small quantity is not a reason for open-bench work.

Open PubChem LCSS record · CID 30318 ↗

Puromycin dihydrochloride

High concern
CAS 58-58-2Formula C₂₂H₃₁Cl₂N₇O₅Type Commercial reagentSignal word Warning

A protein-synthesis inhibitor used for cell selection; powder and concentrated stocks require control for biological activity and acute toxicity.

Cell culture
Acute toxicity
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Representative GHS hazard statements

H302Harmful if swallowed

Handling controls

  • Prefer small packs or premade solution and reconstitute powder in a designated area while preventing aerosol and skin contact.
  • Dispose of drug-containing medium and contaminated materials under institutional cytotoxic or pharmaceutical-waste procedures.

Critical incompatibilities

Strong oxidizers; use product instructions and SDS for stock solvent, pH, and stability.

Boundary commonly missed

Puromycin free base and the dihydrochloride have different CAS numbers and formulas; the salt form must match the search.

Open PubChem LCSS record · CID 443311 ↗

Sodium deoxycholate

High concern
CAS 302-95-4Formula C₂₄H₃₉NaO₄Type Pure substanceSignal word Warning

An anionic detergent used in RIPA, cell lysis, and bile-salt systems; dust can irritate the respiratory tract and it is harmful if swallowed.

ProteinNucleic acidGeneral
Acute toxicityIrritant / sensitizer
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Representative GHS hazard statements

H302Harmful if swallowed
H335May cause respiratory irritation

Handling controls

  • Use local exhaust during powder weighing, prevent aerosols, and clean slippery solution residues.
  • Mixed lysis buffers such as RIPA must also be managed for SDS, inhibitors, and other detergents in the formulation.

Critical incompatibilities

Strong oxidizers; acidification can form deoxycholic acid with different solubility and properties.

Boundary commonly missed

Bile salt does not mean physiologically harmless; assess neat powder, buffer concentration, and commercial RIPA formulation separately.

Open PubChem LCSS record · CID 23668196 ↗

Sodium cacodylate trihydrate

Critical concern
CAS 6131-99-3Formula C₂H₁₂AsNaO₅Type Pure substanceSignal word Danger

An arsenic-containing electron-microscopy buffer that is toxic by inhalation or ingestion and carries carcinogenicity and long-term aquatic hazards.

HistologyGeneral
Acute toxicityChronic hazard
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Representative GHS hazard statements

H301Toxic if swallowed
H331Toxic if inhaled
H351Suspected of causing cancer
H410Very toxic to aquatic life with long lasting effects

Handling controls

  • Weigh powder and prepare stocks in a designated area under local exhaust using dedicated tools and secondary containment.
  • Route every arsenic-containing buffer, specimen, and contaminated item to a dedicated hazardous-waste stream; never drain-dispose as ordinary buffer.

Critical incompatibilities

Strong oxidizers, strong acids, and strong bases; avoid conditions that generate or release other toxic arsenic compounds.

Boundary commonly missed

The trihydrate and anhydrous sodium cacodylate have different CAS numbers and formulas, but both require arsenic hazardous-waste controls.

Open PubChem LCSS record · CID 23679059 ↗

Sodium acetate trihydrate

Routine priority
CAS 6131-90-4Formula C₂H₉NaO₅Type Pure substanceSignal word No uniform signal word

A salt used for nucleic-acid precipitation and buffers; the cited trihydrate source does not provide a uniform GHS H-code set, but dust, acidification, and concentrated solutions still require good control.

Nucleic acidProteinGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Minimize dust during weighing, prevent eye contact, and verify whether the bottle contains anhydrous material or trihydrate.
  • When adjusting pH with glacial acetic acid, apply its corrosive, volatile, and incompatibility controls.

Critical incompatibilities

Strong oxidizers; acidified systems should also be kept from strong bases and hypochlorite.

Boundary commonly missed

The trihydrate (CAS 6131-90-4) and anhydrous sodium acetate (CAS 127-09-3) have different molecular weights and recipe masses are not interchangeable.

Open PubChem LCSS record · CID 23665404 ↗

Magnesium chloride hexahydrate

Routine priority
CAS 7791-18-6Formula Cl₂H₁₂MgO₆Type Pure substanceSignal word No uniform signal word

A magnesium salt used in enzyme reactions and media; the cited hexahydrate source does not provide a uniform GHS H-code set, but hygroscopic powder, eye contact, and concentrated salt solutions still require control.

Nucleic acidProteinCell cultureGeneral
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Representative GHS hazard statements

This source does not provide one set of H-codes that applies to every form or concentration. Open the source and check the current product SDS.

Handling controls

  • Weigh promptly and reseal to reduce moisture-driven concentration error and powder spread.
  • Use eye protection for concentrated stocks and clean crystallized or splashed residue.

Critical incompatibilities

Strong oxidizers; concentrated phosphate, carbonate, or alkaline systems can produce precipitates.

Boundary commonly missed

Hexahydrate (MW 203.30) and anhydrous magnesium chloride (MW 95.21) cannot use the same recipe mass; hydrate state and product can change the SDS classification.

Open PubChem LCSS record · CID 24644 ↗

IPTG

High concern
CAS 367-93-1Formula C₉H₁₈O₅SType Pure substanceSignal word Warning

A chemical inducer used for lac-system expression and blue-white screening; some sources aggregated by PubChem classify it as harmful by ingestion, skin contact, and inhalation.

ProteinCell cultureGeneral
Acute toxicity
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Representative GHS hazard statements

H302Harmful if swallowed
H312Harmful in contact with skin
H332Harmful if inhaled

Handling controls

  • Prevent dust and skin contact during weighing and use eye and hand protection.
  • Filter, aliquot, and freeze-thaw stocks according to the product instructions and label concentration clearly.

Critical incompatibilities

Strong oxidizers; use Section 10 of the supplier SDS for product-specific incompatibilities.

Boundary commonly missed

IPTG GHS classifications vary among suppliers; this page shows a representative aggregation and actual work must follow the current label and product SDS.

Open PubChem LCSS record · CID 656894 ↗
LCSS → SDS → local controls

How to use this information

  • H-codes and signal words are representative classifications from PubChem LCSS/GHS records; regulatory sources may not be fully identical.
  • Pure substances, solution concentrations, and commercial mixtures can carry different labels. For real work, the container label, current supplier SDS, and institutional risk assessment take precedence.
  • PPE is not a fixed checklist: glove material and change interval depend on chemical, concentration, contact time, and SDS permeation data.
  • This catalog does not provide individualized medical advice or replace occupational limits, waste rules, or laboratory safety training.
Content reviewed: 2026-08-31

For research and laboratory hazard recognition only; not medical advice. In an emergency, contact local emergency services, poison control, and institutional EHS.