{"schema_version":"5.0","benchmark_id":"qpcr-primerbank-mouse-transcriptome-v0.5","snapshot_date":"2026-09-02","scope":"Joint fixed-GRCm39 genomic and accessioned RefSeq RNA paired-amplicon screen","source_benchmark":{"benchmark_id":"qpcr-primerbank-mouse-genome-v0.4","file_sha256":"27b53fee18844613486ba09680113ad57c03cd14d7a021a87ff7428bbe0a2ba0","case_payload_sha256":"06f0e755601c77473fdfb19f612e0d0ec0857ba68a406b09596b5d30f3a00e06"},"reference":{"path":"/Volumes/本地磁盘/primercat-reference/GCF_000001635.27_GRCm39/primercat-reference-manifest.json","sha256":"d2f17589f3ef1d0df29460285d989e9c65e000060b2d72a6c9f9a94230d1d3f6","assembly_accession":"GCF_000001635.27","assembly_name":"GRCm39","annotation_release":"GCF_000001635.27-RS_2024_02","source":"NCBI RefSeq","base_url":"https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/635/GCF_000001635.27_GRCm39","bowtie2_build_version":"/opt/homebrew/Cellar/bowtie2/2.5.5/bin/bowtie2-build-s version 2.5.5","transcriptome_fasta_source_md5":"232bb68742e56492ce7b0f22b63800cd","transcriptome_fasta_sha256":"0d1191aa7ed46bdc0160e4770f326e47491fcf35b848294e62419045d9eb9323","transcript_locus_database_sha256":"2848bda4867421c3003930a6525b84bb592987c54de10970573cbc442fb5b57e"},"runtime":{"biopython":"1.88","max_transcript_alignments_per_primer":128,"transcript_amplicon_size_range_bp":[50,5000],"max_mismatches_per_primer":2,"source_code_sha256":{"backend/app/services/primer_transcriptome.py":"783d96d9ee5747b6debab669efec92e1eacde11333f3eee63c4735e8f05b49a7","backend/app/services/primer_bowtie2.py":"34b6db4e0aea0c3745aadb9bcb50abb94af874e2196a965874d6b403c885b909","backend/app/services/qpcr_target_locus.py":"3ae4d4e9335cf939c301496b155d86af5ff14f3f5acda5be7d60bb5126e9b2c3","backend/app/services/gene_primer_service.py":"a1210c9fb7bfb9227f166b95535f9f25e732b975488c91ee6a974e4a13e677eb","backend/app/services/primer_scoring.py":"2734777fb09c36f013d047ad0e9c867f3af3b06b72b07d6ac236ab4e4ef88e2f","backend/benchmarks/build_qpcr_transcriptome_v0_5.py":"399ad158db83a3b607816bfae3c421d540093bf74ed31cea0aa4a27fd6e4c11c"}},"decision_rule":"A gene-level joint pass requires exactly one product on the selected RefSeq transcript, no product assigned to another gene or an unclassified transcript, no transcript hit-cap truncation, and a compatible genomic result: either exactly one target-locus product with no additional genomic product, or no contiguous genomic product at all. Same-gene isoform products are reported separately and prevent only the isoform-specific flag.","limitations":["The fixed RNA FASTA contains accessioned products annotated on this assembly release; it is not a sample-specific expressed transcriptome.","Gene-level and isoform-level conclusions depend on the fixed RefSeq annotation and may change with another release.","The reference does not represent sample-specific variants, structural variation, or unannotated transcripts.","A computational pass does not establish qPCR efficiency, linearity, sensitivity, or a single wet-lab product."],"results":{"source_records":100,"candidate_pairs_screened":1000,"records_with_at_least_one_combined_pass":94,"combined_computational_pass_pairs":864,"transcript_gene_specific_pairs":914,"transcript_isoform_specific_pairs":339,"pairs_with_one_target_transcript_product":979,"pairs_amplifying_same_gene_isoform":592,"pairs_with_cross_gene_product":85,"pairs_with_unclassified_transcript_product":0,"pairs_with_no_transcript_product":0,"transcript_hit_cap_reached_pairs":6,"no_contiguous_genomic_product_rescued_by_transcript_evidence":203,"v0_4_genome_pass_rejected_by_transcript_evidence":4,"transcript_status_counts":{"target_not_found":20,"truncated":6,"validated":974}},"cases":[{"primerbank_id":"28316762a1","gene":"Vmn2r5","target_transcript":"NM_001385126.1","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":0,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGCGGTGAAGATGACTGGTC","right":"AAGACCACAAGTGCCCCAAA","transcript_product_size_bp":131,"primer3_penalty":0.0699,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":5,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":6,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399074,"end":64399204,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64024685,"end":64024815,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247818,"end":64247948,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64540826,"end":64540956,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64167214,"end":64167344,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":12,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1774,"end":1904,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NM_001385025.1","start":1765,"end":1895,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r2","gene_name":"Vmn2r2"},{"transcript_accession":"NR_003962.1","start":1888,"end":2018,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"},{"transcript_accession":"XM_017319412.3","start":1356,"end":1486,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r2","gene_name":"Vmn2r2"},{"transcript_accession":"XM_017319413.2","start":1578,"end":1708,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r2","gene_name":"Vmn2r2"},{"transcript_accession":"XM_030252330.2","start":1789,"end":1919,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r2","gene_name":"Vmn2r2"},{"transcript_accession":"NM_001384949.1","start":2441,"end":2571,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r3","gene_name":"Vmn2r3"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"GGACAACCCCTATGCTGCTT","right":"GACCAGTCATCTTCACCGCA","transcript_product_size_bp":104,"primer3_penalty":0.0714,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":6,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399185,"end":64399976,"product_size":792,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540937,"end":64541718,"product_size":782,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247929,"end":64248729,"product_size":801,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":3,"right_hit_count":12,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1690,"end":1793,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1804,"end":1907,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"},{"transcript_accession":"NM_001384949.1","start":2357,"end":2460,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r3","gene_name":"Vmn2r3"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"ACCCCTATGCTGCTTTGCTT","right":"GACCAGTCATCTTCACCGCA","transcript_product_size_bp":99,"primer3_penalty":0.0775,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399185,"end":64399971,"product_size":787,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540937,"end":64541713,"product_size":777,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247929,"end":64248724,"product_size":796,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":3,"right_hit_count":12,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1695,"end":1793,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1809,"end":1907,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":4,"left":"AACCCCTATGCTGCTTTGCT","right":"GACCAGTCATCTTCACCGCA","transcript_product_size_bp":100,"primer3_penalty":0.0775,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399185,"end":64399972,"product_size":788,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540937,"end":64541714,"product_size":778,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247929,"end":64248725,"product_size":797,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":2,"right_hit_count":12,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1694,"end":1793,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1808,"end":1907,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"GGACAACCCCTATGCTGCTT","right":"CCCCAGTGCCTCTTCGTAAG","transcript_product_size_bp":171,"primer3_penalty":0.1422,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399118,"end":64399976,"product_size":859,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540870,"end":64541718,"product_size":849,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247862,"end":64248729,"product_size":868,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1690,"end":1860,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1804,"end":1974,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"TGCGGTGAAGATGACTGGTC","right":"CCCCAGTGCCTCTTCGTAAG","transcript_product_size_bp":87,"primer3_penalty":0.1455,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":5,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":6,"right_hit_count":4,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399118,"end":64399204,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540870,"end":64540956,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64011917,"end":64012003,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000069.7","start":64247862,"end":64247948,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":12,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1774,"end":1860,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1888,"end":1974,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"},{"transcript_accession":"NM_001384924.1","start":1767,"end":1853,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"},{"transcript_accession":"NM_019918.2","start":1777,"end":1863,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"},{"transcript_accession":"XM_006501757.4","start":4021,"end":4107,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"},{"transcript_accession":"XM_006501758.1","start":2197,"end":2283,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"},{"transcript_accession":"XM_006501759.4","start":2073,"end":2159,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":7,"left":"ACCCCTATGCTGCTTTGCTT","right":"CCCCAGTGCCTCTTCGTAAG","transcript_product_size_bp":166,"primer3_penalty":0.1483,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399118,"end":64399971,"product_size":854,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540870,"end":64541713,"product_size":844,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247862,"end":64248724,"product_size":863,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1695,"end":1860,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1809,"end":1974,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"},{"transcript_accession":"XM_006501758.1","start":2118,"end":2283,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn2r1","gene_name":"Vmn2r1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"AACCCCTATGCTGCTTTGCT","right":"CCCCAGTGCCTCTTCGTAAG","transcript_product_size_bp":167,"primer3_penalty":0.1483,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399118,"end":64399972,"product_size":855,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540870,"end":64541714,"product_size":845,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247862,"end":64248725,"product_size":864,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1694,"end":1860,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1808,"end":1974,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"CAACCCCTATGCTGCTTTGC","right":"GACCAGTCATCTTCACCGCA","transcript_product_size_bp":101,"primer3_penalty":0.2115,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399185,"end":64399973,"product_size":789,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540937,"end":64541715,"product_size":779,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247929,"end":64248726,"product_size":798,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":2,"right_hit_count":12,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1693,"end":1793,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1807,"end":1907,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"GGACAACCCCTATGCTGCTT","right":"ACAAGAGTGAACCCCAGTGC","transcript_product_size_bp":182,"primer3_penalty":0.2131,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_locus_accession":"NC_000069.7","target_locus_start":64398242,"target_locus_end":64417156,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":64399107,"end":64399976,"product_size":870,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000069.7","start":64540859,"end":64541718,"product_size":860,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":64247851,"end":64248729,"product_size":879,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001385126.1","target_gene_id":"Vmn2r5","target_gene_name":"Vmn2r5","left_hit_count":2,"right_hit_count":11,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001385126.1","start":1690,"end":1871,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn2r5","gene_name":"Vmn2r5"},{"transcript_accession":"NR_003962.1","start":1804,"end":1985,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn2r-ps11","gene_name":"Vmn2r-ps11"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"26337769a1","gene":"Akap17b","target_transcript":"NM_001419768.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":123,"primer3_penalty":0.0753,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887488,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1091,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1045,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGCCCTGTAAGTGGTTTGCA","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":154,"primer3_penalty":0.1452,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887519,"product_size":154,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":938,"end":1091,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":892,"end":1045,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CTCGTGGCTTGAGGAGGAAG","right":"AAGCATGTGTTTCGGGGCTA","transcript_product_size_bp":88,"primer3_penalty":0.146,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35874992,"end":35875079,"product_size":88,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":2910,"end":2997,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":2864,"end":2951,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001419769.1","start":2095,"end":2182,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ATGACAGGTGGGAGCTTTGG","right":"GAGCTTTGCCATCGTCTCCT","transcript_product_size_bp":148,"primer3_penalty":0.1467,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887238,"end":35887385,"product_size":148,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":1072,"end":1219,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":1026,"end":1173,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ATGACAGGTGGGAGCTTTGG","right":"AGAGCTTTGCCATCGTCTCC","transcript_product_size_bp":149,"primer3_penalty":0.1467,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887237,"end":35887385,"product_size":149,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":1072,"end":1220,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":1026,"end":1174,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CTTCTCGGTAGGGGTCAAGC","transcript_product_size_bp":101,"primer3_penalty":0.2126,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887388,"end":35887488,"product_size":101,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1069,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1023,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CTCGGTAGGGGTCAAGCATG","transcript_product_size_bp":98,"primer3_penalty":0.2156,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887391,"end":35887488,"product_size":98,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":4,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1066,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1020,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGCACCTCTACCTCAAGCC","right":"GTGTTTCAGCCTCACCCTCA","transcript_product_size_bp":199,"primer3_penalty":0.2165,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887721,"end":35887919,"product_size":199,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":538,"end":736,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":492,"end":690,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ACGTGTTGCTCCCAGAATGT","right":"GTGTTTCAGCCTCACCCTCA","transcript_product_size_bp":159,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887721,"end":35887879,"product_size":159,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":578,"end":736,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":532,"end":690,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AGTGGAGAGAAGCCCTGTGA","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":122,"primer3_penalty":0.2177,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":30,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887487,"product_size":122,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":11,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":970,"end":1091,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":924,"end":1045,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"9790041a1","gene":"Ube2a","target_transcript":"NM_019668.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"ACCCTGGTATATGCCCGACT","right":"TGTTCCATCCTCAAACGGGG","transcript_product_size_bp":194,"primer3_penalty":0.1427,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":154,"end":347,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":154,"end":347,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACCCTGGTATATGCCCGACT","right":"GTTCCATCCTCAAACGGGGT","transcript_product_size_bp":193,"primer3_penalty":0.1429,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":154,"end":346,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":154,"end":346,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TCTACTCTGGGGTGGTGCTT","right":"AGTCGGGCATATACCAGGGT","transcript_product_size_bp":140,"primer3_penalty":0.284,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36137981,"end":36138120,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":34,"end":173,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":34,"end":173,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TATATGCCCGACTGCTGAGC","right":"TGTTCCATCCTCAAACGGGG","transcript_product_size_bp":187,"primer3_penalty":0.356,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":161,"end":347,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":161,"end":347,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TATATGCCCGACTGCTGAGC","right":"GTTCCATCCTCAAACGGGGT","transcript_product_size_bp":186,"primer3_penalty":0.3562,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":161,"end":346,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":161,"end":346,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GACCCTGGTATATGCCCGAC","right":"TGTTCCATCCTCAAACGGGG","transcript_product_size_bp":195,"primer3_penalty":0.3583,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":153,"end":347,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":153,"end":347,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTGGTATATGCCCGACTGC","right":"TGTTCCATCCTCAAACGGGG","transcript_product_size_bp":192,"primer3_penalty":0.3584,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":156,"end":347,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":156,"end":347,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GACCCTGGTATATGCCCGAC","right":"GTTCCATCCTCAAACGGGGT","transcript_product_size_bp":194,"primer3_penalty":0.3584,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36138100,"end":36138849,"product_size":750,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":2,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":153,"end":346,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":153,"end":346,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCTGGTATATGCCCGACTGC","right":"GTTCCATCCTCAAACGGGGT","transcript_product_size_bp":191,"primer3_penalty":0.3585,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":156,"end":346,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":156,"end":346,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCCTGGTATATGCCCGACTG","right":"TGTTCCATCCTCAAACGGGG","transcript_product_size_bp":193,"primer3_penalty":0.3593,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_locus_accession":"NC_000086.8","target_locus_start":36137948,"target_locus_end":36147875,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019668.4","target_gene_id":"Ube2a","target_gene_name":"Ube2a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019668.4","start":155,"end":347,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2a","gene_name":"Ube2a"},{"transcript_accession":"NM_001313696.1","start":155,"end":347,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2a","gene_name":"Ube2a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6678001a1","gene":"Slc1a1","target_transcript":"NM_009199.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"TTCCGCTTTGCCTTGGGTAT","right":"CGGAATGTAACTGGCAGGGT","transcript_product_size_bp":86,"primer3_penalty":0.0745,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882076,"end":28882759,"product_size":684,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1044,"end":1129,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TTCCGCTTTGCCTTGGGTAT","right":"ACAGCGGAATGTAACTGGCA","transcript_product_size_bp":90,"primer3_penalty":0.0752,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882076,"end":28882763,"product_size":688,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1044,"end":1133,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ACCCTGCCAGTTACATTCCG","right":"GATGGTGACGATCTGCCCAA","transcript_product_size_bp":186,"primer3_penalty":0.1431,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882740,"end":28882925,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1110,"end":1295,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGCCAGTTACATTCCGCTGT","right":"GATGGTGACGATCTGCCCAA","transcript_product_size_bp":182,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882744,"end":28882925,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1114,"end":1295,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACCCTGCCAGTTACATTCCG","right":"GTCCAAGCCATTCAGTTGCG","transcript_product_size_bp":159,"primer3_penalty":0.145,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882740,"end":28882898,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1110,"end":1268,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCAGTTACATTCCGCTGT","right":"GTCCAAGCCATTCAGTTGCG","transcript_product_size_bp":155,"primer3_penalty":0.1458,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882744,"end":28882898,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1114,"end":1268,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCAAGCTGGGCCTTTACATG","right":"CGGAATGTAACTGGCAGGGT","transcript_product_size_bp":187,"primer3_penalty":0.2096,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28880157,"end":28882759,"product_size":2603,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":943,"end":1129,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCAAGCTGGGCCTTTACATG","right":"ACAGCGGAATGTAACTGGCA","transcript_product_size_bp":191,"primer3_penalty":0.2103,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28880157,"end":28882763,"product_size":2607,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":943,"end":1133,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCAAGCTGGGCCTTTACATG","right":"ATACCCAAGGCAAAGCGGAA","transcript_product_size_bp":121,"primer3_penalty":0.2132,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28880157,"end":28882095,"product_size":1939,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":943,"end":1063,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTTCCGCTTTGCCTTGGGTA","right":"CGGAATGTAACTGGCAGGGT","transcript_product_size_bp":87,"primer3_penalty":0.2144,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_locus_accession":"NC_000085.7","target_locus_start":28812535,"target_locus_end":28891360,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":28882075,"end":28882759,"product_size":685,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009199.3","target_gene_id":"Slc1a1","target_gene_name":"Slc1a1","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009199.3","start":1043,"end":1129,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a1","gene_name":"Slc1a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31541872a1","gene":"Ube2q1","target_transcript":"NM_027315.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":3,"candidates":[{"rank":1,"left":"AATCATACCCTGCTGTGCCC","right":"CACCAGCCTCTCCAAGACAG","transcript_product_size_bp":80,"primer3_penalty":0.1431,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683385,"end":89683464,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":432,"end":511,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":554,"end":633,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TTGGCTGCTGTCTTGGAGAG","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":118,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683438,"end":89683885,"product_size":448,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":485,"end":602,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":607,"end":724,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CTTGGCTGCTGTCTTGGAGA","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":119,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683437,"end":89683885,"product_size":449,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":484,"end":602,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":606,"end":724,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCCACTGCAACATCACGGAA","right":"TCTCCAAGACAGCAGCCAAG","transcript_product_size_bp":90,"primer3_penalty":0.1435,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":414,"end":503,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TCCACTGCAACATCACGGAA","right":"CTCTCCAAGACAGCAGCCAA","transcript_product_size_bp":91,"primer3_penalty":0.1435,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":5,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":414,"end":504,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTGTCTTGGAGAGGCTGGTG","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":111,"primer3_penalty":0.1439,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683445,"end":89683885,"product_size":441,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":492,"end":602,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":614,"end":724,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGACCCTAACTTGGCTGCTG","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":128,"primer3_penalty":0.144,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683428,"end":89683885,"product_size":458,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":475,"end":602,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":597,"end":724,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ACCCTAACTTGGCTGCTGTC","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":126,"primer3_penalty":0.1442,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683430,"end":89683885,"product_size":456,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":477,"end":602,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":599,"end":724,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GACCCTAACTTGGCTGCTGT","right":"CAGGATGCTGAGGGAGGTTG","transcript_product_size_bp":127,"primer3_penalty":0.1442,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89683429,"end":89683885,"product_size":457,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":476,"end":602,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"},{"transcript_accession":"XM_036163299.1","start":598,"end":724,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCCACTGCAACATCACGGAA","right":"CACCAGCCTCTCCAAGACAG","transcript_product_size_bp":98,"primer3_penalty":0.1442,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_locus_accession":"NC_000069.7","target_locus_start":89680916,"target_locus_end":89691304,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027315.4","target_gene_id":"Ube2q1","target_gene_name":"Ube2q1","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027315.4","start":414,"end":511,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2q1","gene_name":"Ube2q1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"7305335a1","gene":"Nufip1","target_transcript":"NM_013745.6","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGCCAGAGATTCGAAGCCTG","right":"AGGCTGGTGTCACTTCCTTG","transcript_product_size_bp":137,"primer3_penalty":0.2173,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363695,"end":76368312,"product_size":4618,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":954,"end":1090,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":954,"end":1090,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1132,"end":1268,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1021,"end":1157,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":969,"end":1105,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":949,"end":1085,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGCCAGAGATTCGAAGCCTG","right":"CAAGGCTGGTGTCACTTCCT","transcript_product_size_bp":139,"primer3_penalty":0.2173,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363695,"end":76368314,"product_size":4620,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":954,"end":1092,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":954,"end":1092,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1132,"end":1270,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1021,"end":1159,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":969,"end":1107,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":949,"end":1087,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCCAGAGATTCGAAGCCTGA","right":"AGGCTGGTGTCACTTCCTTG","transcript_product_size_bp":136,"primer3_penalty":0.286,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363696,"end":76368312,"product_size":4617,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":955,"end":1090,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":955,"end":1090,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1133,"end":1268,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1022,"end":1157,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":970,"end":1105,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":950,"end":1085,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GCCAGAGATTCGAAGCCTGA","right":"CAAGGCTGGTGTCACTTCCT","transcript_product_size_bp":138,"primer3_penalty":0.286,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363696,"end":76368314,"product_size":4619,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":955,"end":1092,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":955,"end":1092,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1133,"end":1270,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1022,"end":1159,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":970,"end":1107,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":950,"end":1087,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGCCAGAGATTCGAAGCCTG","right":"TCCTTGGGCACTACAGTCCT","transcript_product_size_bp":123,"primer3_penalty":0.2869,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363695,"end":76368298,"product_size":4604,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":954,"end":1076,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":954,"end":1076,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1132,"end":1254,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1021,"end":1143,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":969,"end":1091,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":949,"end":1071,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCAGAGATTCGAAGCCTG","right":"GTGTCACTTCCTTGGGCACT","transcript_product_size_bp":131,"primer3_penalty":0.287,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363695,"end":76368306,"product_size":4612,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":954,"end":1084,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":954,"end":1084,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1132,"end":1262,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1021,"end":1151,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":969,"end":1099,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":949,"end":1079,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGCCAGAGATTCGAAGCCTG","right":"ACAAGGCTGGTGTCACTTCC","transcript_product_size_bp":140,"primer3_penalty":0.287,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363695,"end":76368315,"product_size":4621,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":954,"end":1093,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":954,"end":1093,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1132,"end":1271,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1021,"end":1160,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":969,"end":1108,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":949,"end":1088,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCACCGTTCGATGCTCAGAT","right":"CGTGGAGGCTTGCAAATTCC","transcript_product_size_bp":117,"primer3_penalty":0.2882,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76348586,"end":76348702,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":250,"end":366,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCCAGAGATTCGAAGCCTGA","right":"TCCTTGGGCACTACAGTCCT","transcript_product_size_bp":122,"primer3_penalty":0.3556,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363696,"end":76368298,"product_size":4603,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":955,"end":1076,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":955,"end":1076,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1133,"end":1254,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1022,"end":1143,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":970,"end":1091,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":950,"end":1071,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCCAGAGATTCGAAGCCTGA","right":"GTGTCACTTCCTTGGGCACT","transcript_product_size_bp":130,"primer3_penalty":0.3557,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_locus_accession":"NC_000080.7","target_locus_start":76348337,"target_locus_end":76372527,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":76363696,"end":76368306,"product_size":4611,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013745.6","target_gene_id":"Nufip1","target_gene_name":"Nufip1","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013745.6","start":955,"end":1084,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NM_001424636.1","start":955,"end":1084,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188811.1","start":1133,"end":1262,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188812.1","start":1022,"end":1151,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188813.1","start":970,"end":1099,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"},{"transcript_accession":"NR_188814.1","start":950,"end":1079,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Nufip1","gene_name":"Nufip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6755548a1","gene":"Slc27a2","target_transcript":"NM_011978.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CCAAAAGCGGCAACCATCAA","right":"AAGTAGCCCCAACCACGATG","transcript_product_size_bp":163,"primer3_penalty":0.0687,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":825,"end":987,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGTGAACTGCTTCGGTACC","right":"TGCCTTCAGTGGATGCGTAG","transcript_product_size_bp":167,"primer3_penalty":0.1425,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":1067,"end":1233,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CATCGTGGTTGGGGCTACTT","right":"CGGTCATTTGGTTTCTGCGG","transcript_product_size_bp":150,"primer3_penalty":0.1452,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":968,"end":1117,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CATCGTGGTTGGGGCTACTT","right":"CCGGTCATTTGGTTTCTGCG","transcript_product_size_bp":151,"primer3_penalty":0.1452,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":968,"end":1118,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCAAAAGCGGCAACCATCAA","right":"CTGGCTGGCTGAGAATTTGC","transcript_product_size_bp":195,"primer3_penalty":0.206,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":126406603,"end":126409707,"product_size":3105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":825,"end":1019,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTGAATGTGTATGGCGTGCC","right":"GGTACTCCGCGATGTGTTGA","transcript_product_size_bp":121,"primer3_penalty":0.2077,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":1659,"end":1779,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGGTGAACTGCTTCGGTACC","right":"GCCTTCAGTGGATGCGTAGA","transcript_product_size_bp":166,"primer3_penalty":0.2101,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":1067,"end":1232,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCCTTGCTATGTCGAGTGGA","right":"AAGTAGCCCCAACCACGATG","transcript_product_size_bp":117,"primer3_penalty":0.2112,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":871,"end":987,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AGGCCTTGCTATGTCGAGTG","right":"AAGTAGCCCCAACCACGATG","transcript_product_size_bp":119,"primer3_penalty":0.2121,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":869,"end":987,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGGCCTTGCTATGTCGAGT","right":"AAGTAGCCCCAACCACGATG","transcript_product_size_bp":120,"primer3_penalty":0.2121,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_locus_accession":"NC_000068.8","target_locus_start":126394944,"target_locus_end":126430163,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011978.2","target_gene_id":"Slc27a2","target_gene_name":"Slc27a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011978.2","start":868,"end":987,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc27a2","gene_name":"Slc27a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6754618a1","gene":"Ccndbp1","target_transcript":"NM_001355127.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GAAGACAACTCCGACAGCCA","right":"CAGCCACCAAGATCCGGATT","transcript_product_size_bp":163,"primer3_penalty":0.1405,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":7,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120843225,"end":120843387,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":682,"end":844,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":682,"end":844,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":710,"end":872,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":544,"end":706,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGAAGACAACTCCGACAGCC","right":"CAGCCACCAAGATCCGGATT","transcript_product_size_bp":164,"primer3_penalty":0.1405,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":7,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120843224,"end":120843387,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":681,"end":844,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":681,"end":844,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":709,"end":872,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":543,"end":706,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGCCATCTCCACAGGAAAC","right":"CCGTACCAGCTTTCTCAGGG","transcript_product_size_bp":123,"primer3_penalty":0.1463,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":7,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":292,"end":414,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":292,"end":414,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":292,"end":414,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":126,"end":248,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGGAAGCCACTGTTCTGACA","right":"CCGTACCAGCTTTCTCAGGG","transcript_product_size_bp":164,"primer3_penalty":0.2167,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120839548,"end":120841940,"product_size":2393,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":7,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":251,"end":414,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":251,"end":414,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":251,"end":414,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":85,"end":248,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTACGGTCACTTCTGCCTCG","right":"CGTTCACTTTCACTGCTGCC","transcript_product_size_bp":112,"primer3_penalty":0.2201,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120839063,"end":120839547,"product_size":485,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":139,"end":250,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":139,"end":250,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":139,"end":250,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTACGGTCACTTCTGCCTCG","right":"CCGTTCACTTTCACTGCTGC","transcript_product_size_bp":113,"primer3_penalty":0.2201,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120839063,"end":120839548,"product_size":486,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":139,"end":251,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":139,"end":251,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":139,"end":251,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GAAGACAACTCCGACAGCCA","right":"TTCTCAGCCACCAAGATCCG","transcript_product_size_bp":167,"primer3_penalty":0.2837,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":7,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120843225,"end":120843391,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":682,"end":848,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":682,"end":848,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":710,"end":876,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":544,"end":710,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TGAAGACAACTCCGACAGCC","right":"TTCTCAGCCACCAAGATCCG","transcript_product_size_bp":168,"primer3_penalty":0.2837,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":7,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120843224,"end":120843391,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":681,"end":848,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":681,"end":848,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":709,"end":876,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":543,"end":710,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AATCCGGATCTTGGTGGCTG","right":"AGAGCCTCTGAGTGTCCACA","transcript_product_size_bp":178,"primer3_penalty":0.286,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120843368,"end":120845020,"product_size":1653,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":825,"end":1002,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"XM_011239329.3","start":687,"end":864,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTACGGTCACTTCTGCCTCG","right":"TGTCAGAACAGTGGCTTCCC","transcript_product_size_bp":132,"primer3_penalty":0.2877,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_locus_accession":"NC_000068.8","target_locus_start":120838925,"target_locus_end":120847373,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":120839063,"end":120839567,"product_size":505,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001355127.2","target_gene_id":"Ccndbp1","target_gene_name":"Ccndbp1","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001355127.2","start":139,"end":270,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NM_010761.3","start":139,"end":270,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"},{"transcript_accession":"NR_185170.1","start":139,"end":270,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ccndbp1","gene_name":"Ccndbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"19527004a1","gene":"Ube2s","target_transcript":"NM_133777.2","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GGCCAAGAAACATGCAGGTG","right":"CCCCACCCCAGCCATAATTT","transcript_product_size_bp":163,"primer3_penalty":0.0692,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811076,"end":4811238,"product_size":163,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345485,"end":56345647,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":763,"end":925,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":2,"left":"GGCCAAGAAACATGCAGGTG","right":"GGAAGGAGAAAGAGCCCCAC","transcript_product_size_bp":104,"primer3_penalty":0.0729,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":6,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811135,"end":4811238,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345485,"end":56345588,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":763,"end":866,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":3,"left":"TTGGACAAGAAGCGAGCACT","right":"CCCCACCCCAGCCATAATTT","transcript_product_size_bp":111,"primer3_penalty":0.1379,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811076,"end":4811186,"product_size":111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345537,"end":56345647,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":815,"end":925,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"AGTTGGACAAGAAGCGAGCA","right":"CCCCACCCCAGCCATAATTT","transcript_product_size_bp":113,"primer3_penalty":0.1379,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811076,"end":4811188,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345535,"end":56345647,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":813,"end":925,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":5,"left":"ATGAAGAGTATGCTGCCCGG","right":"TCGCTCACCTGCATGTTTCT","transcript_product_size_bp":191,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811214,"end":4811404,"product_size":191,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345319,"end":56345509,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":597,"end":787,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":6,"left":"ATGAAGAGTATGCTGCCCGG","right":"TCTCGCTCACCTGCATGTTT","transcript_product_size_bp":193,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811212,"end":4811404,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345319,"end":56345511,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":597,"end":789,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"ACATGTGCTGCTGACCATCA","right":"CCGGGCAGCATACTCTTCAT","transcript_product_size_bp":112,"primer3_penalty":0.1446,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":56345227,"end":56345338,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":505,"end":616,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":8,"left":"ATGAAGAGTATGCTGCCCGG","right":"CACCTGCATGTTTCTTGGCC","transcript_product_size_bp":186,"primer3_penalty":0.1454,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811219,"end":4811404,"product_size":186,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345319,"end":56345504,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":597,"end":782,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":9,"left":"CCCAATGGCGAGATCTGTGT","right":"CCGGGCAGCATACTCTTCAT","transcript_product_size_bp":174,"primer3_penalty":0.215,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":4811385,"end":4813495,"product_size":2111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000075.7","start":56345165,"end":56345338,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":443,"end":616,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"TGTGCTGCTGACCATCAAGT","right":"CCGGGCAGCATACTCTTCAT","transcript_product_size_bp":109,"primer3_penalty":0.2166,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_locus_accession":"NC_000073.7","target_locus_start":4811013,"target_locus_end":4815339,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":56345230,"end":56345338,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133777.2","target_gene_id":"Ube2s","target_gene_name":"Ube2s","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133777.2","start":508,"end":616,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2s","gene_name":"Ube2s"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false}]},{"primerbank_id":"11967935a1","gene":"Slc13a2","target_transcript":"NM_022411.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"ACCGTGCTCATCCTCTCTCT","right":"GCATGACATAGAGCGGGTGA","transcript_product_size_bp":133,"primer3_penalty":0.1385,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289218,"end":78289953,"product_size":736,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1427,"end":1559,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":862,"end":994,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1577,"end":1709,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACCGTGCTCATCCTCTCTCT","right":"AGCATGACATAGAGCGGGTG","transcript_product_size_bp":134,"primer3_penalty":0.1392,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289217,"end":78289953,"product_size":737,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1427,"end":1560,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":862,"end":995,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1577,"end":1710,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CAAGGCCAGGTCAACTCGAT","right":"TCCTCTTCCCCTTCCCCAAA","transcript_product_size_bp":179,"primer3_penalty":0.1396,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":9,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":791,"end":969,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532645.4","start":837,"end":1015,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":838,"end":1119,"product_size":282,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CAAGGCCAGGTCAACTCGAT","right":"TTCCTCTTCCCCTTCCCCAA","transcript_product_size_bp":180,"primer3_penalty":0.1396,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":11,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":5,"right_hit_count":8,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":791,"end":970,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532645.4","start":837,"end":1016,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":838,"end":1120,"product_size":283,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACCGTGCTCATCCTCTCTCT","right":"GCATGAAAGCTAGGGAGGCA","transcript_product_size_bp":169,"primer3_penalty":0.1406,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289182,"end":78289953,"product_size":772,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1427,"end":1595,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":862,"end":1030,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1577,"end":1745,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAAGGCCAGGTCAACTCGAT","right":"CGGAAGTTGACACCCAGGAA","transcript_product_size_bp":146,"primer3_penalty":0.1438,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":791,"end":936,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532645.4","start":837,"end":982,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAAGGCCAGGTCAACTCGAT","right":"TTCCGGAAGTTGACACCCAG","transcript_product_size_bp":149,"primer3_penalty":0.1438,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":791,"end":939,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532645.4","start":837,"end":985,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CACCGTGCTCATCCTCTCTC","right":"GCATGACATAGAGCGGGTGA","transcript_product_size_bp":134,"primer3_penalty":0.207,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289218,"end":78289954,"product_size":737,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1426,"end":1559,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":861,"end":994,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1576,"end":1709,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CACCGTGCTCATCCTCTCTC","right":"AGCATGACATAGAGCGGGTG","transcript_product_size_bp":135,"primer3_penalty":0.2077,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289217,"end":78289954,"product_size":738,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1426,"end":1560,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":861,"end":995,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1576,"end":1710,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CACCGTGCTCATCCTCTCTC","right":"GCATGAAAGCTAGGGAGGCA","transcript_product_size_bp":170,"primer3_penalty":0.2091,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_locus_accession":"NC_000077.7","target_locus_start":78288102,"target_locus_end":78313060,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":78289182,"end":78289954,"product_size":773,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_022411.4","target_gene_id":"Slc13a2","target_gene_name":"Slc13a2","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_022411.4","start":1426,"end":1595,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XM_006532648.4","start":861,"end":1030,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"},{"transcript_accession":"XR_003949367.1","start":1576,"end":1745,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc13a2","gene_name":"Slc13a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"7595942a1","gene":"Tas2r116","target_transcript":"NM_053212.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CAGCACTAAGGCCCACATCA","right":"CCAGAAGCAGAGCACTCCAA","transcript_product_size_bp":110,"primer3_penalty":0.071,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833063,"end":132833172,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":663,"end":772,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCACATCAGAGCCTTGCAGA","right":"CCAGAAGCAGAGCACTCCAA","transcript_product_size_bp":98,"primer3_penalty":0.0716,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833075,"end":132833172,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":675,"end":772,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CAGCACTAAGGCCCACATCA","right":"GTGCACTGACGGAAAAGCTG","transcript_product_size_bp":169,"primer3_penalty":0.0761,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833063,"end":132833231,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":663,"end":831,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCACATCAGAGCCTTGCAGA","right":"GTGCACTGACGGAAAAGCTG","transcript_product_size_bp":157,"primer3_penalty":0.0767,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833075,"end":132833231,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":675,"end":831,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CAGCACTAAGGCCCACATCA","right":"GAATCAGGACCCAGGAGTGC","transcript_product_size_bp":185,"primer3_penalty":0.1426,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833063,"end":132833247,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":663,"end":847,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CCACATCAGAGCCTTGCAGA","right":"GAATCAGGACCCAGGAGTGC","transcript_product_size_bp":173,"primer3_penalty":0.1432,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833075,"end":132833247,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":675,"end":847,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAGCACTAAGGCCCACATCA","right":"CTCCAGAAGCAGAGCACTCC","transcript_product_size_bp":112,"primer3_penalty":0.1434,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833063,"end":132833174,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":663,"end":774,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCACATCAGAGCCTTGCAGA","right":"CTCCAGAAGCAGAGCACTCC","transcript_product_size_bp":100,"primer3_penalty":0.144,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833075,"end":132833174,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":675,"end":774,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGCACTAAGGCCCACATCA","right":"AGTGCACTGACGGAAAAGCT","transcript_product_size_bp":170,"primer3_penalty":0.2142,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833063,"end":132833232,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":663,"end":832,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCACATCAGAGCCTTGCAGA","right":"AGTGCACTGACGGAAAAGCT","transcript_product_size_bp":158,"primer3_penalty":0.2148,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_locus_accession":"NC_000072.7","target_locus_start":132832401,"target_locus_end":132833318,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":132833075,"end":132833232,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_053212.1","target_gene_id":"Tas2r116","target_gene_name":"Tas2r116","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_053212.1","start":675,"end":832,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tas2r116","gene_name":"Tas2r116"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26343989a1","gene":"Akap17b","target_transcript":"NM_001419768.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":123,"primer3_penalty":0.0753,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887488,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1091,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1045,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGCCCTGTAAGTGGTTTGCA","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":154,"primer3_penalty":0.1452,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887519,"product_size":154,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":938,"end":1091,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":892,"end":1045,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CTCGTGGCTTGAGGAGGAAG","right":"AAGCATGTGTTTCGGGGCTA","transcript_product_size_bp":88,"primer3_penalty":0.146,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35874992,"end":35875079,"product_size":88,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":2910,"end":2997,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":2864,"end":2951,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001419769.1","start":2095,"end":2182,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ATGACAGGTGGGAGCTTTGG","right":"GAGCTTTGCCATCGTCTCCT","transcript_product_size_bp":148,"primer3_penalty":0.1467,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887238,"end":35887385,"product_size":148,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":1072,"end":1219,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":1026,"end":1173,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ATGACAGGTGGGAGCTTTGG","right":"AGAGCTTTGCCATCGTCTCC","transcript_product_size_bp":149,"primer3_penalty":0.1467,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887237,"end":35887385,"product_size":149,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":1072,"end":1220,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":1026,"end":1174,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CTTCTCGGTAGGGGTCAAGC","transcript_product_size_bp":101,"primer3_penalty":0.2126,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887388,"end":35887488,"product_size":101,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1069,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1023,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAGTGGAGAGAAGCCCTGTG","right":"CTCGGTAGGGGTCAAGCATG","transcript_product_size_bp":98,"primer3_penalty":0.2156,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887391,"end":35887488,"product_size":98,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":4,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":969,"end":1066,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":923,"end":1020,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGCACCTCTACCTCAAGCC","right":"GTGTTTCAGCCTCACCCTCA","transcript_product_size_bp":199,"primer3_penalty":0.2165,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887721,"end":35887919,"product_size":199,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":538,"end":736,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":492,"end":690,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ACGTGTTGCTCCCAGAATGT","right":"GTGTTTCAGCCTCACCCTCA","transcript_product_size_bp":159,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887721,"end":35887879,"product_size":159,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":578,"end":736,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":532,"end":690,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AGTGGAGAGAAGCCCTGTGA","right":"CCAAAGCTCCCACCTGTCAT","transcript_product_size_bp":122,"primer3_penalty":0.2177,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_locus_accession":"NC_000086.8","target_locus_start":35871832,"target_locus_end":35909021,"target_locus_strand":"-","left_hit_count":30,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":35887366,"end":35887487,"product_size":122,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001419768.1","target_gene_id":"Akap17b","target_gene_name":"Akap17b","left_hit_count":11,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001419768.1","start":970,"end":1091,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Akap17b","gene_name":"Akap17b"},{"transcript_accession":"NM_001081956.2","start":924,"end":1045,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Akap17b","gene_name":"Akap17b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"27769357a1","gene":"Madd","target_transcript":"NM_001177720.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GTGCTGACTCCACTCCTGAC","right":"GGCTAAGCACACACAGGGAT","transcript_product_size_bp":108,"primer3_penalty":0.0728,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":91008360,"end":91008467,"product_size":108,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":51,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":518,"end":625,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":770,"end":877,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":770,"end":877,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":773,"end":880,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GTGCTGACTCCACTCCTGAC","right":"AGCACACACAGGGATGTCAG","transcript_product_size_bp":103,"primer3_penalty":0.0737,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":91008365,"end":91008467,"product_size":103,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":52,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":518,"end":620,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":770,"end":872,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":770,"end":872,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":773,"end":875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATGTCTGTGATGGCGTTCGT","right":"AGGAAGAAGCTGGCAGGAAC","transcript_product_size_bp":149,"primer3_penalty":0.0748,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":91006049,"end":91006596,"product_size":548,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":51,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":1081,"end":1229,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":1333,"end":1481,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":1333,"end":1481,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":1336,"end":1484,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GTTCCTGCCAGCTTCTTCCT","right":"GAATGGGCTGGGTGTTGAGA","transcript_product_size_bp":199,"primer3_penalty":0.0756,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":52,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":1210,"end":1408,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":1462,"end":1660,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":1462,"end":1660,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":1465,"end":1663,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AGTTCCTGCCAGCTTCTTCC","right":"GAATGGGCTGGGTGTTGAGA","transcript_product_size_bp":200,"primer3_penalty":0.0756,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":52,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":1209,"end":1408,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":1461,"end":1660,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":1461,"end":1660,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":1464,"end":1663,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTTCCTGCCAGCTTCTTCCT","right":"AGAATGGGCTGGGTGTTGAG","transcript_product_size_bp":200,"primer3_penalty":0.0762,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":53,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":1210,"end":1409,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":1462,"end":1661,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":1462,"end":1661,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":1465,"end":1664,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCTCTCCCAACTCCACTGTC","right":"CTTTCAGGCTGGGGAATGGT","transcript_product_size_bp":168,"primer3_penalty":0.0771,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":90995711,"end":90996956,"product_size":1246,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":51,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":2567,"end":2734,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":2819,"end":2986,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":2819,"end":2986,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":2822,"end":2989,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCCGAGGAAGATGAGGAGGA","right":"GTCTGAGTCACTTGCCAGCT","transcript_product_size_bp":123,"primer3_penalty":0.1406,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":90996979,"end":90997329,"product_size":351,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":64,"right_hit_count":51,"paired_amplicon_count":29,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":28,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":2422,"end":2544,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":2674,"end":2796,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":2674,"end":2796,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":2677,"end":2799,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCCGAGGAAGATGAGGAGGA","right":"GACAGTGGAGTTGGGAGAGC","transcript_product_size_bp":165,"primer3_penalty":0.1428,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":90996937,"end":90997329,"product_size":393,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":61,"right_hit_count":51,"paired_amplicon_count":29,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":28,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":2422,"end":2586,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":2674,"end":2838,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":2674,"end":2838,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":2677,"end":2841,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ATCCCTGTGTGTGCTTAGCC","right":"TAGCAGCCGTTCACTACAGC","transcript_product_size_bp":100,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_locus_accession":"NC_000068.8","target_locus_start":90967705,"target_locus_end":91009666,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":91008280,"end":91008379,"product_size":100,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001177720.1","target_gene_id":"Madd","target_gene_name":"Madd","left_hit_count":51,"right_hit_count":51,"paired_amplicon_count":51,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":50,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001177720.1","start":606,"end":705,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_001177719.1","start":858,"end":957,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"NM_145527.4","start":858,"end":957,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250202.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250207.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250208.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250209.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250217.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250220.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250225.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250227.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"},{"transcript_accession":"XM_030250236.2","start":861,"end":960,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Madd","gene_name":"Madd"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"30102937a1","gene":"Slco1b2","target_transcript":"NM_020495.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":2,"candidates":[{"rank":1,"left":"TCGGTGGAAGGCATAGGGTA","right":"CTGAATGAGAGGGCTGCCAA","transcript_product_size_bp":194,"primer3_penalty":0.0667,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":3,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":108,"end":301,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCGGTGGAAGGCATAGGGTA","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":166,"primer3_penalty":0.0671,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":108,"end":273,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGTTGTCGGTGGAAGGCATA","right":"CTGAATGAGAGGGCTGCCAA","transcript_product_size_bp":199,"primer3_penalty":0.0706,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":3,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":103,"end":301,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":151,"end":344,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGTTGTCGGTGGAAGGCATA","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":171,"primer3_penalty":0.071,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141578870,"end":141580259,"product_size":1390,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":103,"end":273,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":151,"end":316,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TTGGCAGCCCTCTCATTCAG","right":"GGTCGGTGTAGCTTGGATCC","transcript_product_size_bp":191,"primer3_penalty":0.2138,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141594221,"end":141598223,"product_size":4003,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":282,"end":472,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_006506963.2","start":264,"end":454,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_017321611.3","start":259,"end":449,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":325,"end":515,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AGAAAGCTTGGGTTGTCGGT","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":181,"primer3_penalty":0.2179,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141578860,"end":141580259,"product_size":1400,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":93,"end":273,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":141,"end":316,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TTGTCGGTGGAAGGCATAGG","right":"CTGAATGAGAGGGCTGCCAA","transcript_product_size_bp":197,"primer3_penalty":0.2867,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":3,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":105,"end":301,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":153,"end":344,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TTGTCGGTGGAAGGCATAGG","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":169,"primer3_penalty":0.2871,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141578872,"end":141580259,"product_size":1388,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":105,"end":273,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":153,"end":316,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GAAAGCTTGGGTTGTCGGTG","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":180,"primer3_penalty":0.346,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141578861,"end":141580259,"product_size":1399,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":94,"end":273,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":142,"end":316,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCCAGAAAGCTTGGGTTGTC","right":"TGAATCCATCGCAGTGCCTT","transcript_product_size_bp":184,"primer3_penalty":0.3504,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_locus_accession":"NC_000072.7","target_locus_start":141575244,"target_locus_end":141632372,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":141578857,"end":141580259,"product_size":1403,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020495.2","target_gene_id":"Slco1b2","target_gene_name":"Slco1b2","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020495.2","start":90,"end":273,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco1b2","gene_name":"Slco1b2"},{"transcript_accession":"XM_036166208.1","start":138,"end":316,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco1b2","gene_name":"Slco1b2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"3925719a1","gene":"Arih1","target_transcript":"NM_019927.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TCGGACGAGGGCTACAACTA","right":"GTTATCATCGTCCGGCTCGT","transcript_product_size_bp":111,"primer3_penalty":0.0655,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":59393514,"end":59393624,"product_size":111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":34,"end":144,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":1846,"end":1956,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":1846,"end":1956,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":1846,"end":1956,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGACGAGGGCTACAACTACG","right":"GTTATCATCGTCCGGCTCGT","transcript_product_size_bp":109,"primer3_penalty":0.1305,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":59393514,"end":59393622,"product_size":109,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":36,"end":144,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":1848,"end":1956,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":1848,"end":1956,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":1848,"end":1956,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CGGACGAGGGCTACAACTAC","right":"GTTATCATCGTCCGGCTCGT","transcript_product_size_bp":110,"primer3_penalty":0.1305,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":59393514,"end":59393623,"product_size":110,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":35,"end":144,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":1847,"end":1956,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":1847,"end":1956,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":1847,"end":1956,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CAAGGTCATCGGCACAGGAT","right":"TGCGCAGGACACGAAATAGT","transcript_product_size_bp":172,"primer3_penalty":0.1452,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":551,"end":722,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2295,"end":2466,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2431,"end":2602,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_011242739.3","start":8871,"end":9042,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2363,"end":2534,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACTATTTCGTGTCCTGCGCA","right":"GGGGCAGGACACCACTTTAA","transcript_product_size_bp":155,"primer3_penalty":0.1493,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":703,"end":857,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2447,"end":2601,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2583,"end":2737,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_011242739.3","start":9023,"end":9177,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2515,"end":2669,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCGTGTCAGATCTGCTAC","right":"TGCGCAGGACACGAAATAGT","transcript_product_size_bp":151,"primer3_penalty":0.2112,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":572,"end":722,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2316,"end":2466,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2452,"end":2602,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_011242739.3","start":8892,"end":9042,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2384,"end":2534,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGAGGTCAACGAGGTCATCC","right":"ATCCTGTGCCGATGACCTTG","transcript_product_size_bp":196,"primer3_penalty":0.2835,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":375,"end":570,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2187,"end":2314,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2187,"end":2382,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2187,"end":2450,"product_size":264,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGGAGGTCAACGAGGTCATC","right":"ATCCTGTGCCGATGACCTTG","transcript_product_size_bp":197,"primer3_penalty":0.2835,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":374,"end":570,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2186,"end":2314,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2186,"end":2382,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2186,"end":2450,"product_size":265,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TACAAGGTCATCGGCACAGG","right":"TGCGCAGGACACGAAATAGT","transcript_product_size_bp":174,"primer3_penalty":0.2868,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":6,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":549,"end":722,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":2293,"end":2466,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":2429,"end":2602,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_011242739.3","start":8869,"end":9042,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":2361,"end":2534,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCGGACGAGGGCTACAACTA","right":"TTATCATCGTCCGGCTCGTC","transcript_product_size_bp":110,"primer3_penalty":0.3451,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_locus_accession":"NC_000075.7","target_locus_start":59295837,"target_locus_end":59393657,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":59393515,"end":59393624,"product_size":110,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019927.2","target_gene_id":"Arih1","target_gene_name":"Arih1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019927.2","start":34,"end":143,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511140.4","start":1846,"end":1955,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XM_006511141.3","start":1846,"end":1955,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"},{"transcript_accession":"XR_004935434.1","start":1846,"end":1955,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arih1","gene_name":"Arih1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21717727a1","gene":"Vmn1r233","target_transcript":"NM_134202.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"TGTGCGTCATGATCCAACCA","right":"CTGGATGCAGGGGTGGATTT","transcript_product_size_bp":183,"primer3_penalty":0.0688,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214236,"end":21214418,"product_size":183,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":531,"end":713,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGTGCGTCATGATCCAACCA","right":"GATGCAGGGGTGGATTTGGA","transcript_product_size_bp":180,"primer3_penalty":0.0692,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214239,"end":21214418,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":531,"end":710,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGTGCGTCATGATCCAACCA","right":"GCAGGGGTGGATTTGGAAGA","transcript_product_size_bp":177,"primer3_penalty":0.0756,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214242,"end":21214418,"product_size":177,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":531,"end":707,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GCTGTGCGTCATGATCCAAC","right":"CTGGATGCAGGGGTGGATTT","transcript_product_size_bp":185,"primer3_penalty":0.1305,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214236,"end":21214420,"product_size":185,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":529,"end":713,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCTGTGCGTCATGATCCAAC","right":"GATGCAGGGGTGGATTTGGA","transcript_product_size_bp":182,"primer3_penalty":0.1309,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214239,"end":21214420,"product_size":182,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":529,"end":710,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCTGTGCGTCATGATCCAAC","right":"GCAGGGGTGGATTTGGAAGA","transcript_product_size_bp":179,"primer3_penalty":0.1373,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214242,"end":21214420,"product_size":179,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":529,"end":707,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ATGCTGTGCGTCATGATCCA","right":"CTGGATGCAGGGGTGGATTT","transcript_product_size_bp":187,"primer3_penalty":0.1404,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214236,"end":21214422,"product_size":187,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":527,"end":713,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ATGCTGTGCGTCATGATCCA","right":"GATGCAGGGGTGGATTTGGA","transcript_product_size_bp":184,"primer3_penalty":0.1408,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214239,"end":21214422,"product_size":184,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":527,"end":710,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TGCTATGCTGTGCGTCATGA","right":"CTGGATGCAGGGGTGGATTT","transcript_product_size_bp":191,"primer3_penalty":0.141,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214236,"end":21214426,"product_size":191,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":523,"end":713,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGCTATGCTGTGCGTCATGA","right":"GATGCAGGGGTGGATTTGGA","transcript_product_size_bp":188,"primer3_penalty":0.1414,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_locus_accession":"NC_000083.7","target_locus_start":21213989,"target_locus_end":21214948,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21214239,"end":21214426,"product_size":188,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134202.1","target_gene_id":"Vmn1r233","target_gene_name":"Vmn1r233","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134202.1","start":523,"end":710,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r233","gene_name":"Vmn1r233"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"9910602a1","gene":"Ube2l6","target_transcript":"NM_019949.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CAACGTCAGGGAGGATGGTC","right":"AGGTCAGCAAGTTCCAGACG","transcript_product_size_bp":153,"primer3_penalty":0.1422,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84636788,"end":84639421,"product_size":2634,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":309,"end":461,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AGTCTTTCGAAGGAGCTGCC","right":"CATAGGGAAGCTGGTCTGGC","transcript_product_size_bp":103,"primer3_penalty":0.2159,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":115,"end":217,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGTCTTTCGAAGGAGCTGCC","right":"CGCACTTGGAAGGCTTTGAG","transcript_product_size_bp":125,"primer3_penalty":0.2762,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84633132,"end":84636718,"product_size":3587,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":115,"end":239,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"AACGTCAGGGAGGATGGTCT","right":"AGGTCAGCAAGTTCCAGACG","transcript_product_size_bp":152,"primer3_penalty":0.2863,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84636789,"end":84639421,"product_size":2633,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":310,"end":461,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCCAGACCAGCTTCCCTATG","right":"GACCATCCTCCCTGACGTTG","transcript_product_size_bp":131,"primer3_penalty":0.2872,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":198,"end":328,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTCAAAGCCTTCCAAGTGCG","right":"GACCATCCTCCCTGACGTTG","transcript_product_size_bp":109,"primer3_penalty":0.3475,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84636699,"end":84636807,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":220,"end":328,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGTGACGATGCCAATGTGCT","right":"GACCATCCTCCCTGACGTTG","transcript_product_size_bp":172,"primer3_penalty":0.4301,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84633174,"end":84636807,"product_size":3634,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":157,"end":328,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCCAGACCAGCTTCCCTATG","right":"AGACCATCCTCCCTGACGTT","transcript_product_size_bp":132,"primer3_penalty":0.4313,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":198,"end":329,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTCAAAGCCTTCCAAGTGCG","right":"AGACCATCCTCCCTGACGTT","transcript_product_size_bp":110,"primer3_penalty":0.4916,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84636699,"end":84636808,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":220,"end":329,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAACGTCAGGGAGGATGGTC","right":"CAGGAGGTCAGCAAGTTCCA","transcript_product_size_bp":157,"primer3_penalty":0.5061,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_locus_accession":"NC_000068.8","target_locus_start":84629172,"target_locus_end":84640347,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":84636788,"end":84639425,"product_size":2638,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019949.2","target_gene_id":"Ube2l6","target_gene_name":"Ube2l6","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019949.2","start":309,"end":465,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2l6","gene_name":"Ube2l6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"33469019a1","gene":"Prkrir","target_transcript":"NM_028410.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AACAACCCGCATAGCAGACA","right":"CTGGGGTTCTGAGCATTGGT","transcript_product_size_bp":134,"primer3_penalty":0.0749,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98359399,"end":98364243,"product_size":4845,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":9,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":420,"end":553,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":515,"end":648,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":535,"end":668,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":619,"end":810,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":714,"end":905,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AACAACCCGCATAGCAGACA","right":"ACTGGGGTTCTGAGCATTGG","transcript_product_size_bp":135,"primer3_penalty":0.0749,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98359399,"end":98364244,"product_size":4846,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":420,"end":554,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":515,"end":649,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":535,"end":669,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":619,"end":811,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":714,"end":906,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CCCCAGGCTGTCTATACCCT","right":"TTCCTAAAGCCACGGACACC","transcript_product_size_bp":94,"primer3_penalty":0.1407,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98364875,"end":98364968,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":1185,"end":1278,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":1280,"end":1373,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":1442,"end":1535,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":1300,"end":1393,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":1537,"end":1630,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCCCAGGCTGTCTATACCCT","right":"GGTTCCTAAAGCCACGGACA","transcript_product_size_bp":96,"primer3_penalty":0.1407,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98364875,"end":98364970,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":1185,"end":1280,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":1280,"end":1375,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":1442,"end":1537,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":1300,"end":1395,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":1537,"end":1632,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TCCCCAGGCTGTCTATACCC","right":"TTCCTAAAGCCACGGACACC","transcript_product_size_bp":95,"primer3_penalty":0.1409,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98364874,"end":98364968,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":1184,"end":1278,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":1279,"end":1373,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":1441,"end":1535,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":1299,"end":1393,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":1536,"end":1630,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCCCCAGGCTGTCTATACCC","right":"GGTTCCTAAAGCCACGGACA","transcript_product_size_bp":97,"primer3_penalty":0.1409,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98364874,"end":98364970,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":1184,"end":1280,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":1279,"end":1375,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":1441,"end":1537,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":1299,"end":1395,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":1536,"end":1632,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGATATCGAGCTCCCGTCCA","right":"GTCTGTCAGAGTGTTCCGCA","transcript_product_size_bp":181,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98365786,"end":98365966,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":2096,"end":2276,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":2191,"end":2371,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":2353,"end":2533,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":2211,"end":2391,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":2448,"end":2628,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCCGCATAGCAGACACAGAA","right":"CTGGGGTTCTGAGCATTGGT","transcript_product_size_bp":129,"primer3_penalty":0.1469,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98359404,"end":98364243,"product_size":4840,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":11,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":425,"end":553,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":520,"end":648,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":540,"end":668,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":624,"end":810,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":719,"end":905,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCCGCATAGCAGACACAGAA","right":"ACTGGGGTTCTGAGCATTGG","transcript_product_size_bp":130,"primer3_penalty":0.1469,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98359404,"end":98364244,"product_size":4841,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":425,"end":554,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":520,"end":649,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":540,"end":669,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":624,"end":811,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":719,"end":906,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCAATGCTCAGAACCCCAGT","right":"AGTTGTCTGGGGCAAAGAGG","transcript_product_size_bp":198,"primer3_penalty":0.15,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_locus_accession":"NC_000073.7","target_locus_start":98352454,"target_locus_end":98367269,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":98364225,"end":98364422,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028410.2","target_gene_id":"Thap12","target_gene_name":"Thap12","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028410.2","start":535,"end":732,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"NM_001355114.1","start":630,"end":827,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_006508224.5","start":792,"end":989,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_011241915.4","start":650,"end":847,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"},{"transcript_accession":"XM_036153467.1","start":887,"end":1084,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Thap12","gene_name":"Thap12"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"22003902a1","gene":"Vmn1r192","target_transcript":"NM_145845.1","candidate_pairs_screened":10,"combined_pass_pairs":1,"transcript_gene_specific_pairs":1,"transcript_isoform_specific_pairs":1,"candidates":[{"rank":1,"left":"GCTGCAACATTCATTGGCCA","right":"TCCAGCCCATGAGACTCTGA","transcript_product_size_bp":115,"primer3_penalty":0.0788,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":14,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371624,"end":22371738,"product_size":115,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260442,"end":23260556,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23385660,"end":23385771,"product_size":112,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23321129,"end":23321243,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":481,"end":595,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":481,"end":595,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"},{"transcript_accession":"NM_134222.1","start":475,"end":589,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r218","gene_name":"Vmn1r218"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"GTGTGGTCCAGGCTATCACC","right":"TGGCCAATGAATGTTGCAGC","transcript_product_size_bp":193,"primer3_penalty":0.1448,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":8,"right_hit_count":5,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371719,"end":22371911,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260269,"end":23260461,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23385752,"end":23385944,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":308,"end":500,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":308,"end":500,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"AGCTGCAACATTCATTGGCC","right":"TCCAGCCCATGAGACTCTGA","transcript_product_size_bp":116,"primer3_penalty":0.29,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":14,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371624,"end":22371739,"product_size":116,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23385660,"end":23385772,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23260441,"end":23260556,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23321128,"end":23321243,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":480,"end":595,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":480,"end":595,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"},{"transcript_accession":"NM_134222.1","start":474,"end":589,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r218","gene_name":"Vmn1r218"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":4,"left":"GTGTGGTCCAGGCTATCACC","right":"GGCCAATGAATGTTGCAGCT","transcript_product_size_bp":192,"primer3_penalty":0.3559,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":9,"right_hit_count":5,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371720,"end":22371911,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260269,"end":23260460,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":23385753,"end":23385944,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":308,"end":499,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":308,"end":499,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"AGCATGCCAAGTTCTTCCCT","right":"TGGCCAATGAATGTTGCAGC","transcript_product_size_bp":129,"primer3_penalty":0.4443,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371719,"end":22371847,"product_size":129,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":372,"end":500,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGTCTCCTCAGTGTGGTCCA","right":"CTTGGCATGCTGTTTGTGGT","transcript_product_size_bp":85,"primer3_penalty":0.4958,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":18,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371837,"end":22371921,"product_size":85,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260259,"end":23260343,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":298,"end":382,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":298,"end":382,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":7,"left":"TGTCTCCTCAGTGTGGTCCA","right":"ACTTGGCATGCTGTTTGTGG","transcript_product_size_bp":86,"primer3_penalty":0.4958,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":17,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371836,"end":22371921,"product_size":86,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260259,"end":23260344,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":298,"end":383,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":298,"end":383,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"CTCTCCATCTGCACCACCTG","right":"CTTGGCATGCTGTTTGTGGT","transcript_product_size_bp":103,"primer3_penalty":0.4979,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":27,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371837,"end":22371939,"product_size":103,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260241,"end":23260343,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":17,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":280,"end":382,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":280,"end":382,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"CTCTCCATCTGCACCACCTG","right":"ACTTGGCATGCTGTTTGTGG","transcript_product_size_bp":104,"primer3_penalty":0.4979,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":27,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371836,"end":22371939,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260241,"end":23260344,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":15,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":280,"end":383,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":280,"end":383,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"GCTGCAACATTCATTGGCCA","right":"CTCCAGCCCATGAGACTCTG","transcript_product_size_bp":116,"primer3_penalty":0.502,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_locus_accession":"NC_000079.7","target_locus_start":22371316,"target_locus_end":22372218,"target_locus_strand":"-","left_hit_count":6,"right_hit_count":21,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22371623,"end":22371738,"product_size":116,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23260442,"end":23260557,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23385659,"end":23385771,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":23321129,"end":23321244,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22307944,"end":22308056,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145845.1","target_gene_id":"Vmn1r192","target_gene_name":"Vmn1r192","left_hit_count":4,"right_hit_count":11,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145845.1","start":481,"end":596,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r192","gene_name":"Vmn1r192"},{"transcript_accession":"NM_134219.1","start":481,"end":596,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r215","gene_name":"Vmn1r215"},{"transcript_accession":"NM_134222.1","start":475,"end":590,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r218","gene_name":"Vmn1r218"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"31324545a1","gene":"Rpf1","target_transcript":"NM_027371.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":3,"candidates":[{"rank":1,"left":"GACAAGTTGCCACGTTCCAC","right":"GGCTTGTGGACCCATTCGTA","transcript_product_size_bp":184,"primer3_penalty":0.0647,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":854,"end":1037,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AGACAAGTTGCCACGTTCCA","right":"GGCTTGTGGACCCATTCGTA","transcript_product_size_bp":185,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":853,"end":1037,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGAAGACAAGTTGCCACGT","right":"GGCTTGTGGACCCATTCGTA","transcript_product_size_bp":189,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":849,"end":1037,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CGGAAGGAAAAGTTGGCAGC","right":"TTCATCCGTCGCTTCATCGT","transcript_product_size_bp":171,"primer3_penalty":0.2117,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":250,"end":420,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":250,"end":420,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":255,"end":425,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":256,"end":426,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCGGAAGGAAAAGTTGGCAG","right":"TTCATCCGTCGCTTCATCGT","transcript_product_size_bp":172,"primer3_penalty":0.2117,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":249,"end":420,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":249,"end":420,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":254,"end":425,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":255,"end":426,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAGCGGAAGGAAAAGTTGGC","right":"TTCATCCGTCGCTTCATCGT","transcript_product_size_bp":174,"primer3_penalty":0.2117,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":247,"end":420,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":247,"end":420,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":252,"end":425,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":253,"end":426,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCAGCGGAAGGAAAAGTTGG","right":"TTCATCCGTCGCTTCATCGT","transcript_product_size_bp":175,"primer3_penalty":0.2117,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":246,"end":420,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":246,"end":420,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":251,"end":425,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":252,"end":426,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CGGAAGGAAAAGTTGGCAGC","right":"GTAAACGCGCTGGTTGTCAA","transcript_product_size_bp":111,"primer3_penalty":0.3445,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":250,"end":360,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":250,"end":360,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":255,"end":365,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":256,"end":366,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCGGAAGGAAAAGTTGGCAG","right":"GTAAACGCGCTGGTTGTCAA","transcript_product_size_bp":112,"primer3_penalty":0.3445,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":249,"end":360,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":249,"end":360,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":254,"end":365,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":255,"end":366,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGCGGAAGGAAAAGTTGGC","right":"GTAAACGCGCTGGTTGTCAA","transcript_product_size_bp":114,"primer3_penalty":0.3445,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_locus_accession":"NC_000069.7","target_locus_start":146212099,"target_locus_end":146227178,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027371.3","target_gene_id":"Rpf1","target_gene_name":"Rpf1","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027371.3","start":247,"end":360,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"NM_027332.3","start":247,"end":360,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163302.1","start":252,"end":365,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"},{"transcript_accession":"XM_036163303.1","start":253,"end":366,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Rpf1","gene_name":"Rpf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"30794460a1","gene":"Acbd4","target_transcript":"NM_025988.3","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":0,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGGGATCTGGGACTCCTCAG","right":"AGCCATGTGTCCAACTCCTG","transcript_product_size_bp":153,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996201,"end":102996969,"product_size":769,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1165,"end":1317,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":2,"left":"TGGGATCTGGGACTCCTCAG","right":"CAGCCATGTGTCCAACTCCT","transcript_product_size_bp":154,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996201,"end":102996970,"product_size":770,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1165,"end":1318,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":3,"left":"CTGGGATCTGGGACTCCTCA","right":"AGCCATGTGTCCAACTCCTG","transcript_product_size_bp":154,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996200,"end":102996969,"product_size":770,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1164,"end":1317,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":4,"left":"CTGGGATCTGGGACTCCTCA","right":"CAGCCATGTGTCCAACTCCT","transcript_product_size_bp":155,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996200,"end":102996970,"product_size":771,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1164,"end":1318,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":5,"left":"ACCAATACGAAACGGTGCCT","right":"TTTGACAGTCGGGCTCTTCC","transcript_product_size_bp":169,"primer3_penalty":0.0706,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102993825,"end":102994422,"product_size":598,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":409,"end":577,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":6,"left":"ACCAATACGAAACGGTGCCT","right":"TGGAGCCCTACTTTGAAGCG","transcript_product_size_bp":121,"primer3_penalty":0.0721,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102993825,"end":102994374,"product_size":550,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":409,"end":529,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":7,"left":"CAGTGCCTCCAAGTCCAGAG","right":"CAAACCTGCTGAGCTGAGGA","transcript_product_size_bp":181,"primer3_penalty":0.0725,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":22,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1017,"end":1197,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":8,"left":"GTGCCTCCAAGTCCAGAGTC","right":"CAAACCTGCTGAGCTGAGGA","transcript_product_size_bp":179,"primer3_penalty":0.0727,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1019,"end":1197,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":9,"left":"TCCTCAGCTCAGCAGGTTTG","right":"AGCCATGTGTCCAACTCCTG","transcript_product_size_bp":140,"primer3_penalty":0.0732,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996214,"end":102996969,"product_size":756,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1178,"end":1317,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":10,"left":"TCCTCAGCTCAGCAGGTTTG","right":"CAGCCATGTGTCCAACTCCT","transcript_product_size_bp":141,"primer3_penalty":0.0732,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":102996214,"end":102996970,"product_size":757,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025988.3","target_gene_id":null,"target_gene_name":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NR_169793.1","start":1178,"end":1318,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Acbd4","gene_name":"Acbd4"}],"message":"Transcript 'NM_025988.3' was not present in the transcript locus database."},"combined_computational_pass":false}]},{"primerbank_id":"3766203a1","gene":"Suclg2","target_transcript":"NM_011507.4","candidate_pairs_screened":10,"combined_pass_pairs":3,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGGTGGGATCGTCAACTGTG","right":"TTCTTGGCTGCATCCTCCAG","transcript_product_size_bp":183,"primer3_penalty":0.0695,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799253,"end":155799435,"product_size":183,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":1135,"end":1317,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1231,"end":1413,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1288,"end":1470,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":1619,"end":1801,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1772,"end":1954,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177069.1","start":1617,"end":1799,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":2,"left":"CCAGCGAACTTCTTGGACCT","right":"CACAGTTGACGATCCCACCA","transcript_product_size_bp":130,"primer3_penalty":0.0702,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799416,"end":155799545,"product_size":130,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":1025,"end":1154,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1121,"end":1250,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1178,"end":1307,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":1509,"end":1638,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1662,"end":1791,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177069.1","start":1507,"end":1636,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":3,"left":"AAGCCAGCGAACTTCTTGGA","right":"CACAGTTGACGATCCCACCA","transcript_product_size_bp":133,"primer3_penalty":0.1433,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799416,"end":155799548,"product_size":133,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":1022,"end":1154,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1118,"end":1250,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1175,"end":1307,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":1506,"end":1638,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1659,"end":1791,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177069.1","start":1504,"end":1636,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"GGAACATTGCCTGCTTCGTG","right":"AGGTCCAAGAAGTTCGCTGG","transcript_product_size_bp":97,"primer3_penalty":0.1451,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":5,"right_hit_count":6,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":948,"end":1044,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1044,"end":1140,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1101,"end":1197,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":948,"end":1528,"product_size":581,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1101,"end":1681,"product_size":581,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGCTTCGTGAATGGTGCTG","right":"CACAGTTGACGATCCCACCA","transcript_product_size_bp":197,"primer3_penalty":0.2022,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799416,"end":155799612,"product_size":197,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":4,"right_hit_count":6,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":958,"end":1154,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1054,"end":1250,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1111,"end":1307,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":6,"left":"CTGCTTCGTGAATGGTGCTG","right":"AGGTCCAAGAAGTTCGCTGG","transcript_product_size_bp":87,"primer3_penalty":0.2029,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799526,"end":155799612,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":958,"end":1044,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1054,"end":1140,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1111,"end":1197,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"GGAACATTGCCTGCTTCGTG","right":"TCCAAGAAGTTCGCTGGCTT","transcript_product_size_bp":94,"primer3_penalty":0.2182,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":5,"right_hit_count":6,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":948,"end":1041,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1044,"end":1137,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1101,"end":1194,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":948,"end":1525,"product_size":578,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1101,"end":1678,"product_size":578,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGAACATTGCCTGCTTCGTG","right":"TGGGCTTCCTTTACACCACC","transcript_product_size_bp":121,"primer3_penalty":0.2212,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":5,"right_hit_count":7,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":948,"end":1068,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1044,"end":1164,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1101,"end":1221,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177067.1","start":948,"end":1552,"product_size":605,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NR_177068.1","start":1101,"end":1705,"product_size":605,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGCTTCGTGAATGGTGCTG","right":"TCCAAGAAGTTCGCTGGCTT","transcript_product_size_bp":84,"primer3_penalty":0.2761,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799529,"end":155799612,"product_size":84,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":958,"end":1041,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1054,"end":1137,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1111,"end":1194,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"CTGCTTCGTGAATGGTGCTG","right":"TGGGCTTCCTTTACACCACC","transcript_product_size_bp":111,"primer3_penalty":0.2791,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_locus_accession":"NC_000072.7","target_locus_start":95449992,"target_locus_end":95695799,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":155799502,"end":155799612,"product_size":111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_011507.4","target_gene_id":"Suclg2","target_gene_name":"Suclg2","left_hit_count":3,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_011507.4","start":958,"end":1068,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001326558.2","start":1054,"end":1164,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"},{"transcript_accession":"NM_001410411.1","start":1111,"end":1221,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Suclg2","gene_name":"Suclg2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false}]},{"primerbank_id":"12847575a1","gene":"Ube2cbp","target_transcript":"NM_027394.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TTCCTTGGAAGCTGGCTCTG","right":"CAGAGGGCAGGGTTAAAGGG","transcript_product_size_bp":137,"primer3_penalty":0.0714,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":1000,"end":1136,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":712,"end":848,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GTTCCTTGGAAGCTGGCTCT","right":"CAGAGGGCAGGGTTAAAGGG","transcript_product_size_bp":138,"primer3_penalty":0.0715,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":999,"end":1136,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":711,"end":848,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGTTCCTTGGAAGCTGGCTC","right":"CAGAGGGCAGGGTTAAAGGG","transcript_product_size_bp":139,"primer3_penalty":0.0715,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":998,"end":1136,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":710,"end":848,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAAACTCCTCAGGGTGCTCC","right":"TGCTTTTGGTTCCTGCTCCA","transcript_product_size_bp":181,"primer3_penalty":0.1422,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":6,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":499,"end":679,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418871.1","start":499,"end":679,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":211,"end":391,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418873.1","start":211,"end":391,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGCCAGATGAAGGAGGGATG","right":"GCCACACGAGGAAGGTACAA","transcript_product_size_bp":128,"primer3_penalty":0.1444,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":86333934,"end":86334061,"product_size":128,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":24,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":213,"end":340,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418871.1","start":213,"end":340,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGCCAGATGAAGGAGGGATG","right":"GGTACAAGTCTGACCCCTGC","transcript_product_size_bp":115,"primer3_penalty":0.1464,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":86333947,"end":86334061,"product_size":115,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":24,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":213,"end":327,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418871.1","start":213,"end":327,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AAGTCCTCTACCAGCCCTGT","right":"CAGAGGGCAGGGTTAAAGGG","transcript_product_size_bp":96,"primer3_penalty":0.1514,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":1041,"end":1136,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":753,"end":848,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGAGTGCGCTGCTAATCCTG","right":"GCCACACGAGGAAGGTACAA","transcript_product_size_bp":152,"primer3_penalty":0.2134,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":189,"end":340,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418871.1","start":189,"end":340,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGAGTGCGCTGCTAATCCT","right":"GCCACACGAGGAAGGTACAA","transcript_product_size_bp":153,"primer3_penalty":0.2134,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":188,"end":340,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418871.1","start":188,"end":340,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCTCTGTCCCTTCGCCAAAT","right":"TTCTTGTTCACCCCAGCAGG","transcript_product_size_bp":163,"primer3_penalty":0.214,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_locus_accession":"NC_000075.7","target_locus_start":86189364,"target_locus_end":86347003,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027394.3","target_gene_id":"Ube3d","target_gene_name":"Ube3d","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027394.3","start":1187,"end":1349,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube3d","gene_name":"Ube3d"},{"transcript_accession":"NM_001418872.1","start":899,"end":1061,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube3d","gene_name":"Ube3d"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31980935a1","gene":"Slc1a4","target_transcript":"NM_018861.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"TTGCCCACACATGACCTCTC","right":"GGCATCCCCTTCCACATTCA","transcript_product_size_bp":87,"primer3_penalty":0.0699,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20254461,"end":20256512,"product_size":2052,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":18,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1559,"end":1645,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GAGCAGCCATCTTCCAGTGT","right":"GAGAGGTCATGTGTGGGCAA","transcript_product_size_bp":196,"primer3_penalty":0.0724,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20256493,"end":20257979,"product_size":1487,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":18,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1383,"end":1578,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCCCACACATGACCTCTCTC","right":"GGCATCCCCTTCCACATTCA","transcript_product_size_bp":85,"primer3_penalty":0.1412,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20254461,"end":20256510,"product_size":2050,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1561,"end":1645,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAGCAGCCATCTTCCAGTGT","right":"GAGAGAGGTCATGTGTGGGC","transcript_product_size_bp":198,"primer3_penalty":0.1436,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20256491,"end":20257979,"product_size":1489,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1383,"end":1580,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGTGCTGGTGTCATGGATCA","right":"GGATGACATGGCCCAGCATA","transcript_product_size_bp":146,"primer3_penalty":0.1462,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1018,"end":1163,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGGTGCTGGTGTCATGGATC","right":"GGATGACATGGCCCAGCATA","transcript_product_size_bp":147,"primer3_penalty":0.1462,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1017,"end":1163,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGTGTTCATTGCCCAGCTCA","right":"GAGAGGTCATGTGTGGGCAA","transcript_product_size_bp":168,"primer3_penalty":0.1478,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20256493,"end":20257951,"product_size":1459,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":18,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1411,"end":1578,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGTGTTCATTGCCCAGCTCA","right":"TCCACAATCCAGTCCACAGC","transcript_product_size_bp":198,"primer3_penalty":0.1478,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20256463,"end":20257951,"product_size":1489,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1411,"end":1608,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AGTGTTCATTGCCCAGCTCA","right":"ACAATCCAGTCCACAGCCAG","transcript_product_size_bp":195,"primer3_penalty":0.1484,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20256466,"end":20257951,"product_size":1486,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1411,"end":1605,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTGCCCACACATGACCTCTC","right":"CCCCTTCCACATTCACCACA","transcript_product_size_bp":82,"primer3_penalty":0.149,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_locus_accession":"NC_000077.7","target_locus_start":20252180,"target_locus_end":20282713,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":20254466,"end":20256512,"product_size":2047,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_018861.4","target_gene_id":"Slc1a4","target_gene_name":"Slc1a4","left_hit_count":18,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_018861.3","start":1559,"end":1640,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc1a4","gene_name":"Slc1a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"28893503a1","gene":"Zfp799","target_transcript":"NM_001360479.1","candidate_pairs_screened":10,"combined_pass_pairs":8,"transcript_gene_specific_pairs":8,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TCCCCTTTCCTAGCTCCCTC","right":"ACTGCTTACACACGTAGGGC","transcript_product_size_bp":156,"primer3_penalty":0.0686,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":36,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039415,"end":33039570,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":4,"right_hit_count":22,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":871,"end":1026,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":865,"end":1020,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":868,"end":1023,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":1017,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":933,"product_size":72,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":2,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AAGCAGTGTGGGAAAGCCTT","right":"ACTGCTTACACACGTAGGGC","transcript_product_size_bp":175,"primer3_penalty":0.1436,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":65,"right_hit_count":6,"paired_amplicon_count":9,"target_amplicon_count":2,"off_target_amplicon_count":7,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039415,"end":33039589,"product_size":175,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000083.7","start":33039415,"end":33039673,"product_size":259,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":true},{"accession":"NC_000083.7","start":33016775,"end":33017033,"product_size":259,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":33016775,"end":33017369,"product_size":595,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":32993658,"end":32994504,"product_size":847,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":33237958,"end":33238132,"product_size":175,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":33237958,"end":33238216,"product_size":259,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":33237958,"end":33238300,"product_size":343,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000073.7","start":29528415,"end":29528925,"product_size":511,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"is_target":false}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":129,"right_hit_count":21,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":14,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":852,"end":1026,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":846,"end":1020,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":849,"end":1023,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":843,"end":1017,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_001267583.1","start":1172,"end":1430,"product_size":259,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"NM_183177.3","start":1153,"end":1411,"product_size":259,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"XM_011246439.3","start":1313,"end":1571,"product_size":259,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"XM_017317470.3","start":1795,"end":2053,"product_size":259,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"XM_030249792.2","start":1346,"end":1604,"product_size":259,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"NM_001267583.1","start":836,"end":1430,"product_size":595,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"NM_183177.3","start":817,"end":1411,"product_size":595,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"},{"transcript_accession":"XM_011246439.3","start":977,"end":1571,"product_size":595,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Zfp811","gene_name":"Zfp811"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":3,"left":"TGTGTCCCCGCCATATTGTC","right":"GTTCACAGCCACATCCTCCA","transcript_product_size_bp":163,"primer3_penalty":0.1441,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":17,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":5,"right_hit_count":39,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":44,"end":206,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_001293229.1","start":44,"end":206,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":44,"end":206,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":44,"end":206,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":44,"end":206,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCCCCTTTCCTAGCTCCCTC","right":"AACTACGATCGGTGAAGGCC","transcript_product_size_bp":183,"primer3_penalty":0.2061,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":36,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039388,"end":33039570,"product_size":183,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":871,"end":1053,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":865,"end":1047,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":868,"end":1050,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":1044,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCGCCATATTGTCGTCCTGT","right":"GTTCACAGCCACATCCTCCA","transcript_product_size_bp":156,"primer3_penalty":0.2158,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":17,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":5,"right_hit_count":39,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":51,"end":206,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_001293229.1","start":51,"end":206,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":51,"end":206,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":51,"end":206,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":51,"end":206,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCCTTCCCCTTTCCTAGCTC","right":"ACTGCTTACACACGTAGGGC","transcript_product_size_bp":160,"primer3_penalty":0.2192,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039415,"end":33039574,"product_size":160,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":5,"right_hit_count":18,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":867,"end":1026,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":861,"end":1020,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":864,"end":1023,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":858,"end":1017,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCCTCGGTATCTCTGTCCCC","right":"GTTCACAGCCACATCCTCCA","transcript_product_size_bp":108,"primer3_penalty":0.2221,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":17,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":5,"right_hit_count":40,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":99,"end":206,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_001293229.1","start":99,"end":206,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":99,"end":206,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":99,"end":206,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":99,"end":206,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TCCCCTTTCCTAGCTCCCTC","right":"TCCCACACTGCTTACACACG","transcript_product_size_bp":162,"primer3_penalty":0.2807,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":36,"right_hit_count":12,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039409,"end":33039570,"product_size":162,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":6,"right_hit_count":38,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":871,"end":1032,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":865,"end":1026,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":868,"end":1029,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":1023,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TCCCCTTTCCTAGCTCCCTC","right":"GCCTTCCCACACTGCTTACA","transcript_product_size_bp":166,"primer3_penalty":0.2817,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":36,"right_hit_count":56,"paired_amplicon_count":2,"target_amplicon_count":2,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039405,"end":33039570,"product_size":166,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000083.7","start":33039489,"end":33039570,"product_size":82,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":4,"right_hit_count":129,"paired_amplicon_count":8,"target_transcript_amplicon_count":2,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":871,"end":1036,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_001360479.1","start":871,"end":952,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":865,"end":1030,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":868,"end":1033,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":1027,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":865,"end":946,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":868,"end":949,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":862,"end":943,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":10,"left":"AGCCTTCCCCTTTCCTAGCT","right":"ACTGCTTACACACGTAGGGC","transcript_product_size_bp":161,"primer3_penalty":0.291,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_locus_accession":"NC_000083.7","target_locus_start":33034423,"target_locus_end":33049235,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":33039415,"end":33039575,"product_size":161,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360479.1","target_gene_id":"Zfp799","target_gene_name":"Zfp799","left_hit_count":4,"right_hit_count":22,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360479.1","start":866,"end":1026,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"NM_177359.5","start":860,"end":1020,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246441.4","start":863,"end":1023,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"},{"transcript_accession":"XM_011246442.4","start":857,"end":1017,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Zfp799","gene_name":"Zfp799"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"29423606a1","gene":"Eif4ebp3","target_transcript":"NM_201256.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CACTATACGCCACTACCCCG","right":"CTTGGAGGGTGGTACTGCTG","transcript_product_size_bp":152,"primer3_penalty":0.3491,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797332,"end":36798462,"product_size":1131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":220,"end":371,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CAGCAGTACCACCCTCCAAG","right":"CCAGGAAGAGGTGATGGCAA","transcript_product_size_bp":167,"primer3_penalty":0.3653,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36798443,"end":36798744,"product_size":302,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":352,"end":518,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCCTTCCTGCTGCTATGTC","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":114,"primer3_penalty":0.4206,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797238,"end":36797351,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":126,"end":239,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTGCCATCACCTCTTCCTGG","right":"CCTGGTCTAGCTGCCTCAAC","transcript_product_size_bp":108,"primer3_penalty":0.4372,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36798725,"end":36798832,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":499,"end":606,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GACTGCCTACACCTCAGAGC","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":164,"primer3_penalty":0.4877,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797188,"end":36797351,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":76,"end":239,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTGCCTACACCTCAGAGCTG","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":162,"primer3_penalty":0.4883,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797190,"end":36797351,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":78,"end":239,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGAGCCCTCAAGTTGACTGG","right":"GCTCTGAGGTGTAGGCAGTC","transcript_product_size_bp":87,"primer3_penalty":0.4966,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797121,"end":36797207,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":9,"end":95,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTGCTGCTATGTCCTCGTCC","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":107,"primer3_penalty":0.5623,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797245,"end":36797351,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":133,"end":239,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCTGCTGCTATGTCCTCGTC","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":108,"primer3_penalty":0.5623,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797244,"end":36797351,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":132,"end":239,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTCTTGCCTTCCTGCTGCTA","right":"CGGGGTAGTGGCGTATAGTG","transcript_product_size_bp":118,"primer3_penalty":0.5644,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_locus_accession":"NC_000084.7","target_locus_start":36797113,"target_locus_end":36799377,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":36797234,"end":36797351,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_201256.4","target_gene_id":"Eif4ebp3","target_gene_name":"Eif4ebp3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_201256.4","start":122,"end":239,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif4ebp3","gene_name":"Eif4ebp3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21717721a1","gene":"Vmn1r73","target_transcript":"NM_134203.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"TGGAAATGTGGGAGCTGCAT","right":"GGATAGATTGGGTGGCTCTGT","transcript_product_size_bp":191,"primer3_penalty":1.8113,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490702,"end":11490892,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":519,"end":709,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCAGGTCCCAGGTACAACAA","right":"ATGCAGCTCCCACATTTCCA","transcript_product_size_bp":88,"primer3_penalty":1.8492,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490634,"end":11490721,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":451,"end":538,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGCCCCAACAACTACAGGTG","right":"TTGTTGTACCTGGGACCTGA","transcript_product_size_bp":140,"primer3_penalty":1.9196,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490514,"end":11490653,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":331,"end":470,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTGGAAATGTGGGAGCTGCA","right":"GGATAGATTGGGTGGCTCTGT","transcript_product_size_bp":192,"primer3_penalty":1.9502,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490701,"end":11490892,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":518,"end":709,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TCAGGTCCCAGGTACAACAA","right":"TGCAGCTCCCACATTTCCAA","transcript_product_size_bp":87,"primer3_penalty":1.9881,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490634,"end":11490720,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":451,"end":537,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ACCTGAAAACAGAGCCACCC","right":"GGGGCAAATTATGGGAAAGCA","transcript_product_size_bp":169,"primer3_penalty":2.0158,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490864,"end":11491032,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":681,"end":849,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTGAAAACAGAGCCACCCA","right":"GGGGCAAATTATGGGAAAGCA","transcript_product_size_bp":168,"primer3_penalty":2.0158,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":12,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490865,"end":11491032,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":10,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":682,"end":849,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TGGAAATGTGGGAGCTGCAT","right":"GAGGATAGATTGGGTGGCTCTG","transcript_product_size_bp":193,"primer3_penalty":2.0784,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490702,"end":11490894,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":519,"end":711,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TGGAAATGTGGGAGCTGCAT","right":"TGAGGATAGATTGGGTGGCTC","transcript_product_size_bp":194,"primer3_penalty":2.0902,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490702,"end":11490895,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":519,"end":712,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGGAAATGTGGGAGCTGCAT","right":"AGGATAGATTGGGTGGCTCTG","transcript_product_size_bp":192,"primer3_penalty":2.0933,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_locus_accession":"NC_000073.7","target_locus_start":11490184,"target_locus_end":11491095,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":8,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":11490702,"end":11490893,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134203.1","target_gene_id":"Vmn1r73","target_gene_name":"Vmn1r73","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134203.1","start":519,"end":710,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r73","gene_name":"Vmn1r73"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"18479656a1","gene":"Olfr51","target_transcript":"NM_146909.1","candidate_pairs_screened":10,"combined_pass_pairs":1,"transcript_gene_specific_pairs":1,"transcript_isoform_specific_pairs":1,"candidates":[{"rank":1,"left":"CCAACCTCTCCAGTGTCGAC","right":"CCACATAGCGGTCATAGGCC","transcript_product_size_bp":183,"primer3_penalty":0.3589,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":65,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50897991,"end":50898173,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50882809,"end":50882989,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50860037,"end":50860219,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875720,"end":50875902,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":129,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":191,"end":373,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":191,"end":373,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":191,"end":373,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":2,"left":"CCAACCTCTCCAGTGTCGAC","right":"GGCCACATAGCGGTCATAGG","transcript_product_size_bp":185,"primer3_penalty":0.3589,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":65,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":129,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":191,"end":375,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":191,"end":375,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":191,"end":375,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":3,"left":"CCAACCTCTCCAGTGTCGAC","right":"CACATAGCGGTCATAGGCCA","transcript_product_size_bp":182,"primer3_penalty":0.4309,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":65,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":129,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":191,"end":372,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":191,"end":372,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":191,"end":372,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":4,"left":"CCTCACTGTGGTCTCCCTCT","right":"TGTCACGATTCCTCAGGCTG","transcript_product_size_bp":155,"primer3_penalty":0.4995,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50898532,"end":50898686,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":732,"end":886,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCTCACTGTGGTCTCCCTCT","right":"GTCACGATTCCTCAGGCTGT","transcript_product_size_bp":154,"primer3_penalty":0.4997,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50898532,"end":50898685,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50860578,"end":50860731,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":732,"end":885,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":732,"end":885,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"TGGCCTATGACCGCTATGTG","right":"CATGCAGGTTTGTGATGGCC","transcript_product_size_bp":114,"primer3_penalty":0.5012,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":5,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50898153,"end":50898266,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50860199,"end":50860312,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875882,"end":50875995,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000077.7","start":50882969,"end":50883082,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"truncated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":129,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":353,"end":466,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":353,"end":466,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":353,"end":466,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The transcript alignment return limit was reached; no pass can be assigned."},"combined_computational_pass":false},{"rank":7,"left":"CCAACCTCTCCAGTGTCGAC","right":"CGGTCATAGGCCATCACACT","transcript_product_size_bp":175,"primer3_penalty":0.5039,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":10,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50897991,"end":50898165,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50882809,"end":50882981,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50860037,"end":50860211,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875720,"end":50875894,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":13,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":191,"end":365,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":191,"end":365,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":191,"end":365,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"CCAACCTCTCCAGTGTCGAC","right":"TCACACTCAGGAGGAAGCCA","transcript_product_size_bp":162,"primer3_penalty":0.5099,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":6,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50897991,"end":50898152,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50882809,"end":50882968,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50860037,"end":50860198,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875720,"end":50875881,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":191,"end":352,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":191,"end":352,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":191,"end":352,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"AGTGTGATGGCCTATGACCG","right":"CATGCAGGTTTGTGATGGCC","transcript_product_size_bp":121,"primer3_penalty":0.5742,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":10,"right_hit_count":5,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50898146,"end":50898266,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50860192,"end":50860312,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875875,"end":50875995,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000077.7","start":50882962,"end":50883082,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":11,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":346,"end":466,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":346,"end":466,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":346,"end":466,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"TGGCTTCCTCCTGAGTGTGA","right":"CATGCAGGTTTGTGATGGCC","transcript_product_size_bp":134,"primer3_penalty":0.5803,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_locus_accession":"NC_000077.7","target_locus_start":50897801,"target_locus_end":50898724,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":50898133,"end":50898266,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000077.7","start":50850165,"end":50850298,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50860179,"end":50860312,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000077.7","start":50875862,"end":50875995,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000077.7","start":50882949,"end":50883082,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146909.1","target_gene_id":"Or1ad8","target_gene_name":"Or1ad8","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146909.1","start":333,"end":466,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or1ad8","gene_name":"Or1ad8"},{"transcript_accession":"NM_146910.1","start":333,"end":466,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Or1ad6","gene_name":"Or1ad6"},{"transcript_accession":"NM_146911.1","start":333,"end":466,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or1ad1","gene_name":"Or1ad1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"6678479a1","gene":"Ube2e1","target_transcript":"NM_009455.3","candidate_pairs_screened":10,"combined_pass_pairs":8,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"AGACCGAGAAAGAAGGCAGC","right":"CTGGATCCTCTTAGCGCTGG","transcript_product_size_bp":98,"primer3_penalty":0.0701,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":253,"end":350,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AGCAGACCGAGAAAGAAGGC","right":"CTGGATCCTCTTAGCGCTGG","transcript_product_size_bp":101,"primer3_penalty":0.0701,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":250,"end":350,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGACCGAGAAAGAAGGCAGC","right":"GTGATGTCTGCCAGCTCCTT","transcript_product_size_bp":118,"primer3_penalty":0.0725,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138656,"end":4143292,"product_size":4637,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":253,"end":370,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"AGACCGAGAAAGAAGGCAGC","right":"AAGTGATGTCTGCCAGCTCC","transcript_product_size_bp":120,"primer3_penalty":0.0725,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138656,"end":4143294,"product_size":4639,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000071.7","start":117196938,"end":117197056,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":253,"end":372,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":5,"left":"AGCAGACCGAGAAAGAAGGC","right":"GTGATGTCTGCCAGCTCCTT","transcript_product_size_bp":121,"primer3_penalty":0.0725,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138653,"end":4143292,"product_size":4640,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":250,"end":370,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AGCAGACCGAGAAAGAAGGC","right":"AAGTGATGTCTGCCAGCTCC","transcript_product_size_bp":123,"primer3_penalty":0.0725,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138653,"end":4143294,"product_size":4642,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000071.7","start":117196935,"end":117197056,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":250,"end":372,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"AGACCGAGAAAGAAGGCAGC","right":"CTCTTAGCGCTGGTGGAGAG","transcript_product_size_bp":91,"primer3_penalty":0.139,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138656,"end":4138746,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":253,"end":343,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGCAGACCGAGAAAGAAGGC","right":"CTCTTAGCGCTGGTGGAGAG","transcript_product_size_bp":94,"primer3_penalty":0.139,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":4138653,"end":4138746,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":250,"end":343,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCTCCTCGTCATCTTCGTCC","right":"CTGGATCCTCTTAGCGCTGG","transcript_product_size_bp":128,"primer3_penalty":0.2819,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":223,"end":350,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCAGCTCCTCGTCATCTTCG","right":"CTGGATCCTCTTAGCGCTGG","transcript_product_size_bp":131,"primer3_penalty":0.2821,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_locus_accession":"NC_000080.7","target_locus_start":4137852,"target_locus_end":4186967,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009455.3","target_gene_id":"Ube2e1","target_gene_name":"Ube2e1","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009455.3","start":220,"end":350,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2e1","gene_name":"Ube2e1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26331424a1","gene":"Pilrb2","target_transcript":"NM_001024932.2","candidate_pairs_screened":10,"combined_pass_pairs":5,"transcript_gene_specific_pairs":5,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"ACAAGCACTCAACACCACCA","right":"TCTTGTCCCTTGTGTGCTCC","transcript_product_size_bp":86,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":9,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":573,"end":658,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":573,"end":658,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":573,"end":658,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":636,"end":721,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_377143.4","start":949,"end":1034,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"ACAAGCACTCAACACCACCA","right":"TCCTCTTGTCCCTTGTGTGC","transcript_product_size_bp":89,"primer3_penalty":0.1432,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":9,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":573,"end":661,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":573,"end":661,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":573,"end":661,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":636,"end":724,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_868610.3","start":949,"end":1037,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"ACAAGCACTCAACACCACCA","right":"CCTCTTGTCCCTTGTGTGCT","transcript_product_size_bp":88,"primer3_penalty":0.1439,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":573,"end":660,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":573,"end":660,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":573,"end":660,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":636,"end":723,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_133209.2","start":573,"end":660,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XM_011240933.3","start":949,"end":1036,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XM_011240934.3","start":833,"end":920,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XR_377143.4","start":949,"end":1036,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XR_868610.3","start":949,"end":1036,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":4,"left":"ACAAGCACTCAACACCACCA","right":"TTGTCCCTTGTGTGCTCCAG","transcript_product_size_bp":84,"primer3_penalty":0.2136,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":9,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":573,"end":656,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":573,"end":656,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":573,"end":656,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":636,"end":719,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XM_011240933.3","start":949,"end":1032,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XM_011240934.3","start":833,"end":916,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XR_868610.3","start":949,"end":1032,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"ACAAGCACTCAACACCACCA","right":"CTTGTCCCTTGTGTGCTCCA","transcript_product_size_bp":85,"primer3_penalty":0.2136,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":573,"end":657,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":573,"end":657,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":573,"end":657,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":636,"end":720,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_133209.2","start":573,"end":657,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XM_011240933.3","start":949,"end":1033,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XM_011240934.3","start":833,"end":917,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XR_377143.4","start":949,"end":1033,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"},{"transcript_accession":"XR_868610.3","start":949,"end":1033,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":2,"classification":"other_gene","gene_id":"Pilrb1","gene_name":"Pilrb1"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"TCACCCTGACACAAGCACTC","right":"TCTTGTCCCTTGTGTGCTCC","transcript_product_size_bp":96,"primer3_penalty":0.2153,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":6,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":563,"end":658,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":563,"end":658,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":563,"end":658,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":626,"end":721,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCACCCTGACACAAGCACTC","right":"TCCTCTTGTCCCTTGTGTGC","transcript_product_size_bp":99,"primer3_penalty":0.2153,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":563,"end":661,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":563,"end":661,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":563,"end":661,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":626,"end":724,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTCACCCTGACACAAGCACT","right":"TCTTGTCCCTTGTGTGCTCC","transcript_product_size_bp":97,"primer3_penalty":0.216,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":6,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":4,"right_hit_count":8,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":562,"end":658,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":562,"end":658,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":562,"end":658,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":625,"end":721,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTCACCCTGACACAAGCACT","right":"TCCTCTTGTCCCTTGTGTGC","transcript_product_size_bp":100,"primer3_penalty":0.216,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":562,"end":661,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":562,"end":661,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":562,"end":661,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":625,"end":724,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCACCCTGACACAAGCACTC","right":"CCTCTTGTCCCTTGTGTGCT","transcript_product_size_bp":98,"primer3_penalty":0.2161,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_locus_accession":"NC_000071.7","target_locus_start":137864091,"target_locus_end":137870020,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":5,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001024932.2","target_gene_id":"Pilrb2","target_gene_name":"Pilrb2","left_hit_count":4,"right_hit_count":10,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001024932.2","start":563,"end":660,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NM_001384202.1","start":563,"end":660,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"NR_169051.1","start":563,"end":660,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"},{"transcript_accession":"XR_004942542.1","start":626,"end":723,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pilrb2","gene_name":"Pilrb2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"22203777a1","gene":"Olfr47","target_transcript":"NM_146370.1","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":0,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CCTGTCTGTCCTCAAGCTGG","right":"GAGGAACAGGTGGAGAAGGC","transcript_product_size_bp":186,"primer3_penalty":0.0725,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":40,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213083,"end":43213268,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":42932973,"end":42933158,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43130780,"end":43130965,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43179119,"end":43179304,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194388,"end":43194573,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":6,"right_hit_count":44,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":540,"end":725,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":540,"end":725,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":540,"end":725,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"},{"transcript_accession":"NM_146654.1","start":540,"end":725,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a14","gene_name":"Or2a14"},{"transcript_accession":"NM_146656.1","start":540,"end":725,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a56","gene_name":"Or2a56"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"CCTGTCTGTCCTCAAGCTGG","right":"AAGGCCTTTCTGCGTCTCTC","transcript_product_size_bp":171,"primer3_penalty":0.0734,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":3,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213083,"end":43213253,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":43179119,"end":43179289,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194388,"end":43194558,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":540,"end":710,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":540,"end":710,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":540,"end":710,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"CCTGTCTGTCCTCAAGCTGG","right":"TGGGAGGAACAGGTGGAGAA","transcript_product_size_bp":189,"primer3_penalty":0.1412,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":45,"paired_amplicon_count":6,"target_amplicon_count":1,"off_target_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213083,"end":43213271,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":42932973,"end":42933161,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43093217,"end":43093405,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43130780,"end":43130968,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43179119,"end":43179307,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194388,"end":43194576,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":6,"right_hit_count":46,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"},{"transcript_accession":"NM_146654.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a14","gene_name":"Or2a14"},{"transcript_accession":"NM_146655.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a54","gene_name":"Or2a54"},{"transcript_accession":"NM_146656.1","start":540,"end":728,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a56","gene_name":"Or2a56"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":4,"left":"CCTGTCTGTCCTCAAGCTGG","right":"CTTTCTGCGTCTCTCCCCTG","transcript_product_size_bp":166,"primer3_penalty":0.145,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":4,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213083,"end":43213248,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":43179119,"end":43179284,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194388,"end":43194553,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":540,"end":705,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":540,"end":705,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":540,"end":705,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"CCACCCGCTACACTACACTG","right":"CCAGCTTGAGGACAGACAGG","transcript_product_size_bp":182,"primer3_penalty":0.1454,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":8,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43212921,"end":43213102,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":43194226,"end":43194407,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000072.7","start":43130618,"end":43130799,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000072.7","start":43178957,"end":43179138,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":5,"right_hit_count":6,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":378,"end":559,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":378,"end":559,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":378,"end":559,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"},{"transcript_accession":"NM_146654.1","start":378,"end":559,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Or2a14","gene_name":"Or2a14"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"CTCCTACACACGCATCCTGG","right":"GAGGAACAGGTGGAGAAGGC","transcript_product_size_bp":81,"primer3_penalty":0.215,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":40,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213188,"end":43213268,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":42933078,"end":42933158,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43130885,"end":43130965,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43179224,"end":43179304,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194493,"end":43194573,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":7,"right_hit_count":43,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":645,"end":725,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":645,"end":725,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":645,"end":725,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"},{"transcript_accession":"NM_146654.1","start":645,"end":725,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a14","gene_name":"Or2a14"},{"transcript_accession":"NM_146656.1","start":645,"end":725,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a56","gene_name":"Or2a56"},{"transcript_accession":"NM_146295.1","start":639,"end":719,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a12","gene_name":"Or2a12"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":7,"left":"CCACCCGCTACACTACACTG","right":"ATGAGGTCCACAGAAGGGGA","transcript_product_size_bp":136,"primer3_penalty":0.228,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43212921,"end":43213056,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":43194226,"end":43194361,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":378,"end":513,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":378,"end":513,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"CTCCTACACACGCATCCTGG","right":"TGGGAGGAACAGGTGGAGAA","transcript_product_size_bp":84,"primer3_penalty":0.2838,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":45,"paired_amplicon_count":6,"target_amplicon_count":1,"off_target_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213188,"end":43213271,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":42933078,"end":42933161,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43093322,"end":43093405,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43130885,"end":43130968,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43179224,"end":43179307,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000072.7","start":43194493,"end":43194576,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":6,"right_hit_count":44,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"},{"transcript_accession":"NM_146653.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a51","gene_name":"Or2a51"},{"transcript_accession":"NM_146654.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a14","gene_name":"Or2a14"},{"transcript_accession":"NM_146655.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a54","gene_name":"Or2a54"},{"transcript_accession":"NM_146656.1","start":645,"end":728,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a56","gene_name":"Or2a56"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"GGCCCCTGTGCTTTGTACTA","right":"GAGGAACAGGTGGAGAAGGC","transcript_product_size_bp":103,"primer3_penalty":0.3628,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":41,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43213166,"end":43213268,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":2,"right_hit_count":44,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":623,"end":725,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146656.1","start":623,"end":725,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a56","gene_name":"Or2a56"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"CCACCCGCTACACTACACTG","right":"CATGAGGTCCACAGAAGGGG","transcript_product_size_bp":137,"primer3_penalty":0.363,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_locus_accession":"NC_000072.7","target_locus_start":43212544,"target_locus_end":43213509,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43212921,"end":43213057,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000072.7","start":43194226,"end":43194362,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_146370.1","target_gene_id":"Or2a57","target_gene_name":"Or2a57","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_146370.1","start":378,"end":514,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2a57","gene_name":"Or2a57"},{"transcript_accession":"NM_146369.1","start":378,"end":514,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Or2a20","gene_name":"Or2a20"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"30841021a1","gene":"Arfgap3","target_transcript":"NM_001357756.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AAAGACAGCAGCAGAGACCC","right":"TCTGGGCTTCATCTGTGCTG","transcript_product_size_bp":115,"primer3_penalty":0.0717,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83192319,"end":83194506,"product_size":2188,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":1266,"end":1380,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":1260,"end":1374,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":1263,"end":1377,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":1391,"end":1505,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ATGCATGCAAGTTGGAGGGA","right":"GCTGTTGTACTTGGCGTTGG","transcript_product_size_bp":91,"primer3_penalty":0.0805,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83218751,"end":83220885,"product_size":2135,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":329,"end":419,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":329,"end":419,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":329,"end":419,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":457,"end":547,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATGCATGCAAGTTGGAGGGA","right":"GGCTGTTGTACTTGGCGTTG","transcript_product_size_bp":92,"primer3_penalty":0.0805,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83218750,"end":83220885,"product_size":2136,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":329,"end":420,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":329,"end":420,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":329,"end":420,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":457,"end":548,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CGATGCATGCAAGTTGGAGG","right":"GCTGTTGTACTTGGCGTTGG","transcript_product_size_bp":93,"primer3_penalty":0.1404,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83218751,"end":83220887,"product_size":2137,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":327,"end":419,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":327,"end":419,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":327,"end":419,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":455,"end":547,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CGATGCATGCAAGTTGGAGG","right":"GGCTGTTGTACTTGGCGTTG","transcript_product_size_bp":94,"primer3_penalty":0.1404,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83218750,"end":83220887,"product_size":2138,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":327,"end":420,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":327,"end":420,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":327,"end":420,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":455,"end":548,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GATCAAGACTCTGGCCACCC","right":"CGGTGTTGAAGAGGCTGACT","transcript_product_size_bp":184,"primer3_penalty":0.1415,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":5,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83214754,"end":83218724,"product_size":3971,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":446,"end":629,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":446,"end":629,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":446,"end":629,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":574,"end":757,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCAGACCATTGAGCAGGAGT","right":"AGGAATCGGCACCATCATCC","transcript_product_size_bp":185,"primer3_penalty":0.145,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":11,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83194515,"end":83197783,"product_size":3269,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":1073,"end":1257,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":1070,"end":1251,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":1201,"end":1382,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":1070,"end":1254,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGCACAGATGAAGCCCAGA","right":"CGCCCGGGTTTCAAAATCTG","transcript_product_size_bp":100,"primer3_penalty":0.145,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":1361,"end":1460,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":1355,"end":1454,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":1358,"end":1457,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":1486,"end":1585,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGATGATGGTGCCGATTCCT","right":"TCTGGGCTTCATCTGTGCTG","transcript_product_size_bp":143,"primer3_penalty":0.1451,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":83192319,"end":83194534,"product_size":2216,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":1238,"end":1380,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":1232,"end":1374,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":1235,"end":1377,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":1363,"end":1505,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AAAGACAGCAGCAGAGACCC","right":"CGCCCGGGTTTCAAAATCTG","transcript_product_size_bp":195,"primer3_penalty":0.1453,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":13,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_locus_accession":"NC_000081.7","target_locus_start":83183941,"target_locus_end":83234448,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357756.1","target_gene_id":"Arfgap3","target_gene_name":"Arfgap3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357756.1","start":1266,"end":1460,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_001357757.1","start":1260,"end":1454,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"NM_025445.4","start":1263,"end":1457,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"},{"transcript_accession":"XM_006521254.4","start":1391,"end":1585,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arfgap3","gene_name":"Arfgap3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"30794382a1","gene":"Slc16a10","target_transcript":"NM_001114332.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AGCGCCTTGGACTAGTCAAC","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":121,"primer3_penalty":0.074,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952912,"product_size":121,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":799,"end":919,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":799,"end":919,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":278,"end":398,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":811,"end":931,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACGACGTGGCCTTTTACCTT","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":129,"primer3_penalty":0.2132,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913373,"product_size":129,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1552,"end":1680,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1031,"end":1159,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1596,"end":1724,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCAGCAGCGTCTTCACAATC","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":85,"primer3_penalty":0.216,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952876,"product_size":85,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":835,"end":919,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":835,"end":919,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":314,"end":398,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":847,"end":931,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACTTCATGACAAGCTGGGCA","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":152,"primer3_penalty":0.217,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913396,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1529,"end":1680,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1008,"end":1159,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1573,"end":1724,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCCGTCACTGGTCATTTTGG","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":156,"primer3_penalty":0.2777,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952947,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":764,"end":919,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":764,"end":919,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":243,"end":398,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":776,"end":931,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GAAGCGCCTTGGACTAGTCA","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":123,"primer3_penalty":0.2834,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952914,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":797,"end":919,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":797,"end":919,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":276,"end":398,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":809,"end":931,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACGTGGCCTTTTACCTTGCT","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":126,"primer3_penalty":0.285,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913370,"product_size":126,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":15,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1555,"end":1680,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1034,"end":1159,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1599,"end":1724,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TTCAGGAGTTGGACGGCTTC","right":"TCGAAGAGACCCATGGCAAG","transcript_product_size_bp":174,"primer3_penalty":0.2851,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1223,"end":1396,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":702,"end":875,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1267,"end":1440,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTTCAGGAGTTGGACGGCTT","right":"TCGAAGAGACCCATGGCAAG","transcript_product_size_bp":175,"primer3_penalty":0.2857,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1222,"end":1396,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":701,"end":875,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1266,"end":1440,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGCCATGGGTCTCTTCGATG","right":"AAGGTAAAAGGCCACGTCGT","transcript_product_size_bp":193,"primer3_penalty":0.286,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913354,"end":39916740,"product_size":3387,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1379,"end":1571,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":858,"end":1050,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1423,"end":1615,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"28278296a1","gene":"Gtpbp1","target_transcript":"NM_013818.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AGTGAAGCTGACATGGAGGC","right":"TTTTGCCAGCATCCACGTTG","transcript_product_size_bp":196,"primer3_penalty":0.0675,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":79591213,"end":79591930,"product_size":718,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":336,"end":531,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":1119,"end":1314,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":367,"end":562,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ATGTGGGAGAGGATGGACGA","right":"GCCTCCATGTCAGCTTCACT","transcript_product_size_bp":101,"primer3_penalty":0.0677,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":2,"right_hit_count":8,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":255,"end":355,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":286,"end":386,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATGTGGGAGAGGATGGACGA","right":"CCTCTATCTGCTCCGCCATG","transcript_product_size_bp":142,"primer3_penalty":0.0696,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":255,"end":396,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":286,"end":427,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCTGGGAGTTTGAGGCTGAG","right":"GGGGTCTTGATGAAGCGGAA","transcript_product_size_bp":178,"primer3_penalty":0.0711,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":79601248,"end":79601901,"product_size":654,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":1477,"end":1654,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":2260,"end":2437,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":1508,"end":1685,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCTGGGAGTTTGAGGCTGAG","right":"CCCACAGTGTACCATGGCTT","transcript_product_size_bp":83,"primer3_penalty":0.0738,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":1477,"end":1559,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":2260,"end":2342,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":1508,"end":1590,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TTCCGCTTCATCAAGACCCC","right":"CTGGAGGAGCTTGGTGATGG","transcript_product_size_bp":102,"primer3_penalty":0.1428,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":3,"right_hit_count":5,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":1635,"end":1736,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":2418,"end":2519,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":1666,"end":1767,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTGGGAGTTTGAGGCTGAG","right":"GGATGCTCCCACAGTGTACC","transcript_product_size_bp":90,"primer3_penalty":0.1433,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":79601248,"end":79601813,"product_size":566,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":1477,"end":1566,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":2260,"end":2349,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":1508,"end":1597,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCAGATGTGGGAGAGGATGG","right":"GCCTCCATGTCAGCTTCACT","transcript_product_size_bp":105,"primer3_penalty":0.1438,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":13,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":251,"end":355,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":282,"end":386,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGCAGATGTGGGAGAGGATG","right":"GCCTCCATGTCAGCTTCACT","transcript_product_size_bp":106,"primer3_penalty":0.1438,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":4,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":7,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":250,"end":355,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":281,"end":386,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTCCGCTTCATCAAGACCCC","right":"TGTTGGTTGTCTGGAGGAGC","transcript_product_size_bp":112,"primer3_penalty":0.144,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_locus_accession":"NC_000081.7","target_locus_start":79575097,"target_locus_end":79605680,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":79601882,"end":79603247,"product_size":1366,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_013818.2","target_gene_id":"Gtpbp1","target_gene_name":"Gtpbp1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_013818.2","start":1635,"end":1746,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_006520450.2","start":2418,"end":2529,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"},{"transcript_accession":"XM_036159161.1","start":1666,"end":1777,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gtpbp1","gene_name":"Gtpbp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"28972698a1","gene":"Slc39a10","target_transcript":"NM_172653.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CCCCTAGCCACAAAGACCAG","right":"TCCGGTGAGATGGGAGAGTT","transcript_product_size_bp":109,"primer3_penalty":0.0763,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46871859,"end":46874330,"product_size":2472,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":7,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1130,"end":1238,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1264,"end":1372,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1158,"end":1266,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1060,"end":1168,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11616,"end":11724,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1308,"end":1416,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AACTCTCCCATCTCACCGGA","right":"CACGCCGATGCCCCTATATT","transcript_product_size_bp":167,"primer3_penalty":0.0776,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1219,"end":1385,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1353,"end":1519,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1247,"end":1413,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1149,"end":1315,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11705,"end":11871,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1397,"end":1563,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGTCCGAACTCTCCCATCT","right":"CACGCCGATGCCCCTATATT","transcript_product_size_bp":174,"primer3_penalty":0.0776,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1212,"end":1385,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1346,"end":1519,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1240,"end":1413,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1142,"end":1315,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11698,"end":11871,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1390,"end":1563,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCCCTAGCCACAAAGACCAG","right":"CGGTGAGATGGGAGAGTTCG","transcript_product_size_bp":107,"primer3_penalty":0.1365,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46871861,"end":46874330,"product_size":2470,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1130,"end":1236,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1264,"end":1370,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1158,"end":1264,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1060,"end":1166,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11616,"end":11722,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1308,"end":1414,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CGAACTCTCCCATCTCACCG","right":"CACGCCGATGCCCCTATATT","transcript_product_size_bp":169,"primer3_penalty":0.1378,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1217,"end":1385,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1351,"end":1519,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1245,"end":1413,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1147,"end":1315,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11703,"end":11871,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1395,"end":1563,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GACCGTGTACATAGCCCTGG","right":"CTGGTCTTTGTGGCTAGGGG","transcript_product_size_bp":183,"primer3_penalty":0.1394,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46874311,"end":46874493,"product_size":183,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":967,"end":1149,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1101,"end":1283,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":995,"end":1177,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":897,"end":1079,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11453,"end":11635,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1145,"end":1327,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCGCATACCCACCAGCATTT","right":"GCTTTGATGGGCAGTTCAGC","transcript_product_size_bp":125,"primer3_penalty":0.1435,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46874804,"end":46874928,"product_size":125,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":532,"end":656,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":666,"end":790,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":560,"end":684,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":462,"end":586,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11018,"end":11142,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":710,"end":834,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TTCGCATACCCACCAGCATT","right":"GCTTTGATGGGCAGTTCAGC","transcript_product_size_bp":126,"primer3_penalty":0.1435,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46874804,"end":46874929,"product_size":126,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":531,"end":656,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":665,"end":790,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":559,"end":684,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":461,"end":586,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11017,"end":11142,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":709,"end":834,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GACCAGAGTGAAGACGACCG","right":"TCCGGTGAGATGGGAGAGTT","transcript_product_size_bp":95,"primer3_penalty":0.1509,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46871859,"end":46874316,"product_size":2458,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":1144,"end":1238,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1278,"end":1372,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":1172,"end":1266,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":1074,"end":1168,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11630,"end":11724,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1322,"end":1416,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TACACCAGGTTTCCTCCCCA","right":"CCAGGGCTATGTACACGGTC","transcript_product_size_bp":87,"primer3_penalty":0.2087,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_locus_accession":"NC_000067.7","target_locus_start":46846704,"target_locus_end":46892778,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":46874474,"end":46874560,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172653.3","target_gene_id":"Slc39a10","target_gene_name":"Slc39a10","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172653.3","start":900,"end":986,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356416.1","start":1034,"end":1120,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"NM_001356417.1","start":928,"end":1014,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495932.3","start":830,"end":916,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_006495933.5","start":11386,"end":11472,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"},{"transcript_accession":"XM_036164377.1","start":1078,"end":1164,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc39a10","gene_name":"Slc39a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6679895a1","gene":"Slc37a4","target_transcript":"NM_001357730.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TCCTACATCAGTGCCCTCGA","right":"AGAGGAACATGGATGCTGCC","transcript_product_size_bp":163,"primer3_penalty":0.1403,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44312149,"end":44312799,"product_size":651,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1344,"end":1506,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1344,"end":1506,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1042,"end":1204,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1371,"end":1533,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1042,"end":1204,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1371,"end":1533,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1073,"end":1235,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1375,"end":1537,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1402,"end":1564,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1073,"end":1235,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1402,"end":1564,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":910,"end":1072,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGACAAGGACGATTTGGGG","right":"GGCGCTCATCTGATCTGACA","transcript_product_size_bp":98,"primer3_penalty":0.1413,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44309901,"end":44310625,"product_size":725,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":685,"end":782,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":685,"end":782,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":383,"end":480,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":712,"end":809,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":383,"end":480,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":712,"end":809,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":251,"end":348,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"XM_030244053.2","start":736,"end":833,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":383,"end":511,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":685,"end":813,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":712,"end":840,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":383,"end":511,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCAGCTGGTTACCTGTCAG","right":"AGAGGAACATGGATGCTGCC","transcript_product_size_bp":119,"primer3_penalty":0.1421,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44312193,"end":44312799,"product_size":607,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1388,"end":1506,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1388,"end":1506,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1086,"end":1204,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1415,"end":1533,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1086,"end":1204,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1415,"end":1533,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1117,"end":1235,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1419,"end":1537,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1446,"end":1564,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1117,"end":1235,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1446,"end":1564,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":954,"end":1072,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGTGGAGGAGAGAGAAAGCG","right":"CTTGGGCTGCCATGGTAGAA","transcript_product_size_bp":152,"primer3_penalty":0.2088,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44309632,"end":44309783,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":12,"right_hit_count":13,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":416,"end":567,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":416,"end":567,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":114,"end":265,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":443,"end":594,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":114,"end":265,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":443,"end":594,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":114,"end":265,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":416,"end":567,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":443,"end":594,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":114,"end":265,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":443,"end":594,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"XM_030244053.2","start":467,"end":618,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGACGATTTGGGGCTCATCA","right":"GGCGCTCATCTGATCTGACA","transcript_product_size_bp":91,"primer3_penalty":0.211,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":8,"right_hit_count":13,"paired_amplicon_count":8,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":7,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":692,"end":782,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":692,"end":782,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":390,"end":480,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":719,"end":809,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":390,"end":480,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":719,"end":809,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":258,"end":348,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"XM_030244053.2","start":743,"end":833,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CGCTCTGGACAAGGACGATT","right":"GGCGCTCATCTGATCTGACA","transcript_product_size_bp":103,"primer3_penalty":0.2124,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44309896,"end":44310625,"product_size":730,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":680,"end":782,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":680,"end":782,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":378,"end":480,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":707,"end":809,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":378,"end":480,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":707,"end":809,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":246,"end":348,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"XM_030244053.2","start":731,"end":833,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":378,"end":511,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":680,"end":813,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":707,"end":840,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":378,"end":511,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCTCCTACATCAGTGCCCTC","right":"AGAGGAACATGGATGCTGCC","transcript_product_size_bp":165,"primer3_penalty":0.2126,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44312147,"end":44312799,"product_size":653,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1342,"end":1506,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1342,"end":1506,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1040,"end":1204,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1369,"end":1533,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1040,"end":1204,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1369,"end":1533,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1071,"end":1235,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1373,"end":1537,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1400,"end":1564,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1071,"end":1235,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1400,"end":1564,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":908,"end":1072,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TCCTACATCAGTGCCCTCGA","right":"CGTGACGAGGGTTCCCATAC","transcript_product_size_bp":115,"primer3_penalty":0.2126,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44312149,"end":44312751,"product_size":603,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1344,"end":1458,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1344,"end":1458,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1042,"end":1156,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1371,"end":1485,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1042,"end":1156,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1371,"end":1485,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1073,"end":1187,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1375,"end":1489,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1402,"end":1516,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1073,"end":1187,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1402,"end":1516,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":910,"end":1024,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ATCCCCAACTGCAGCCTTAC","right":"CGGGACCTCATTAGCCACTG","transcript_product_size_bp":142,"primer3_penalty":0.2132,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44313787,"end":44313928,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1929,"end":2070,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1866,"end":2007,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1564,"end":1705,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1956,"end":2097,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1627,"end":1768,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1893,"end":2034,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1658,"end":1799,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1960,"end":2101,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1987,"end":2128,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1595,"end":1736,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1924,"end":2065,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":1432,"end":1573,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ATCCCCAACTGCAGCCTTAC","right":"GTCGGGACCTCATTAGCCAC","transcript_product_size_bp":144,"primer3_penalty":0.2132,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_locus_accession":"NC_000075.7","target_locus_start":44308396,"target_locus_end":44314263,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":44313787,"end":44313930,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001357730.2","target_gene_id":"Slc37a4","target_gene_name":"Slc37a4","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001357730.2","start":1929,"end":2072,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293630.2","start":1866,"end":2009,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001293631.2","start":1564,"end":1707,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001357729.2","start":1956,"end":2099,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378829.1","start":1627,"end":1770,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378830.1","start":1893,"end":2036,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378831.1","start":1658,"end":1801,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378832.1","start":1960,"end":2103,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378833.1","start":1987,"end":2130,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001378834.1","start":1595,"end":1738,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_001411170.1","start":1924,"end":2067,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"},{"transcript_accession":"NM_008063.3","start":1432,"end":1575,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc37a4","gene_name":"Slc37a4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"12838433a1","gene":"Hspb9","target_transcript":"NM_029307.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GAGCAGAGTGTTCACCGACA","right":"ACTGGCCTGTTTGAGCTTCA","transcript_product_size_bp":142,"primer3_penalty":0.2156,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100605069,"end":100605210,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":315,"end":456,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACTTGGTGGTGCGGATAGAC","right":"TGTCGGTGAACACTCTGCTC","transcript_product_size_bp":112,"primer3_penalty":0.2815,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100604977,"end":100605088,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":223,"end":334,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ACTTGGTGGTGCGGATAGAC","right":"CGGTGAACACTCTGCTCCAT","transcript_product_size_bp":109,"primer3_penalty":0.2864,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100604977,"end":100605085,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":223,"end":331,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCTTAGCGTTCTTCCTGCGG","right":"TGCTGCACACCTCGGTTTAA","transcript_product_size_bp":120,"primer3_penalty":0.2878,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100605280,"end":100605399,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":526,"end":645,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GAGCAGAGTGTTCACCGACA","right":"TTGTTTTGTCCCCTGAGCCA","transcript_product_size_bp":107,"primer3_penalty":0.2924,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100605069,"end":100605175,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":315,"end":421,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTAGCTTCCCGATGTCCCAG","right":"GTCTATCCGCACCACCAAGT","transcript_product_size_bp":171,"primer3_penalty":0.3544,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100604826,"end":100604996,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":72,"end":242,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGAACGGAATCAAGTGGCCA","right":"GTCTATCCGCACCACCAAGT","transcript_product_size_bp":139,"primer3_penalty":0.3589,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100604858,"end":100604996,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":104,"end":242,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCTGAACGGAATCAAGTGGC","right":"GTCTATCCGCACCACCAAGT","transcript_product_size_bp":141,"primer3_penalty":0.4184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100604856,"end":100604996,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":102,"end":242,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GAGCAGAGTGTTCACCGACA","right":"GGCACTTGTTTTGTCCCCTG","transcript_product_size_bp":112,"primer3_penalty":0.4212,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100605069,"end":100605180,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":315,"end":426,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGAAGCCCAAACAGACTCGG","right":"TGCTGCACACCTCGGTTTAA","transcript_product_size_bp":91,"primer3_penalty":0.4293,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_locus_accession":"NC_000077.7","target_locus_start":100604755,"target_locus_end":100605401,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":100605309,"end":100605399,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029307.2","target_gene_id":"Hspb9","target_gene_name":"Hspb9","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029307.2","start":555,"end":645,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hspb9","gene_name":"Hspb9"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"22003910a1","gene":"Cables2","target_transcript":"NM_001411357.1","candidate_pairs_screened":10,"combined_pass_pairs":5,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":2,"candidates":[{"rank":1,"left":"TCCCTCCTCCAGTAACTGCA","right":"AGAACAAACCAAGCCCCACA","transcript_product_size_bp":158,"primer3_penalty":0.149,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901602,"end":179901759,"product_size":158,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":17,"right_hit_count":18,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1529,"end":1686,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1446,"end":1603,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":1173,"end":1330,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":894,"end":1051,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1518,"end":1675,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1409,"end":1566,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1337,"end":1494,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":1243,"end":1400,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1544,"end":1701,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1411,"end":1568,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1352,"end":1509,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":1171,"end":1328,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGAGCCTGTGTTCTGTTGG","right":"CCAGCTTCTCACACTCACGT","transcript_product_size_bp":91,"primer3_penalty":0.2117,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":8,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":22,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1228,"end":1318,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GAGAAGCTCGTACTGCAGGG","right":"CCAGCTTCTCACACTCACGT","transcript_product_size_bp":144,"primer3_penalty":0.2118,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":17,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1175,"end":1318,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCCCTCCTCCAGTAACTGCA","right":"GAACAAACCAAGCCCCACAC","transcript_product_size_bp":157,"primer3_penalty":0.2205,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901603,"end":179901759,"product_size":157,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1529,"end":1685,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1446,"end":1602,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":1173,"end":1329,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":894,"end":1050,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1518,"end":1674,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1409,"end":1565,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1337,"end":1493,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":1243,"end":1399,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1544,"end":1700,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1411,"end":1567,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1352,"end":1508,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":1171,"end":1327,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCTTTGAGTTCACGGTGCTC","right":"TTTGGGCTAGAACTGCTGGG","transcript_product_size_bp":104,"primer3_penalty":0.2741,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901830,"end":179901933,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":88320074,"end":88320177,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":17,"right_hit_count":18,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1355,"end":1458,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1272,"end":1375,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":999,"end":1102,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":720,"end":823,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1344,"end":1447,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1235,"end":1338,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1163,"end":1266,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":1069,"end":1172,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1370,"end":1473,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1237,"end":1340,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1178,"end":1281,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":997,"end":1100,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":6,"left":"TGGAGAAGGCCTACGGATCA","right":"ACGACCTTTCCATCTGCTCC","transcript_product_size_bp":135,"primer3_penalty":0.283,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":5,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":88320802,"end":88320936,"product_size":135,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":16,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":589,"end":723,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":517,"end":651,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":589,"end":723,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":589,"end":723,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":589,"end":723,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177232.1","start":589,"end":752,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"CCCTCCTCCAGTAACTGCAC","right":"AGAACAAACCAAGCCCCACA","transcript_product_size_bp":157,"primer3_penalty":0.2831,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901602,"end":179901758,"product_size":157,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":17,"right_hit_count":18,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1530,"end":1686,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1447,"end":1603,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":1174,"end":1330,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":895,"end":1051,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1519,"end":1675,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1410,"end":1566,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1338,"end":1494,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":1244,"end":1400,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1545,"end":1701,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1412,"end":1568,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1353,"end":1509,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":1172,"end":1328,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGAGCAGATGGAAAGGTCGT","right":"CGCTTCCTAGTTCTGTGCCA","transcript_product_size_bp":187,"primer3_penalty":0.2878,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":88320631,"end":88320821,"product_size":191,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":6,"right_hit_count":12,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":704,"end":890,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":632,"end":818,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":704,"end":890,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":704,"end":890,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":704,"end":890,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177232.1","start":733,"end":919,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":9,"left":"GCTTTGGAACTGGCCCTGTA","right":"TTTGGGCTAGAACTGCTGGG","transcript_product_size_bp":81,"primer3_penalty":0.2893,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901830,"end":179901910,"product_size":81,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":88320074,"end":88320154,"product_size":81,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":22,"right_hit_count":18,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1378,"end":1458,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1295,"end":1375,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":1022,"end":1102,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":743,"end":823,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1367,"end":1447,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1258,"end":1338,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1186,"end":1266,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":1092,"end":1172,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1393,"end":1473,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1260,"end":1340,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1201,"end":1281,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":1020,"end":1100,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"TGGAGCCTGTGTTCTGTTGG","right":"GGTACAGGGCCAGTTCCAAA","transcript_product_size_bp":172,"primer3_penalty":0.2907,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_locus_accession":"NC_000068.8","target_locus_start":179900332,"target_locus_end":179915258,"target_locus_strand":"-","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":179901889,"end":179902140,"product_size":252,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":88320133,"end":88320293,"product_size":161,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001411357.1","target_gene_id":"Cables2","target_gene_name":"Cables2","left_hit_count":22,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001411357.1","start":1228,"end":1399,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411354.1","start":1156,"end":1316,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411361.1","start":883,"end":1043,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_001411365.1","start":604,"end":764,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NM_145851.2","start":1228,"end":1388,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177224.1","start":1119,"end":1279,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177225.1","start":1047,"end":1207,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177226.1","start":953,"end":1113,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177227.1","start":1254,"end":1414,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177228.1","start":1121,"end":1281,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177229.1","start":1062,"end":1222,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"},{"transcript_accession":"NR_177230.1","start":881,"end":1041,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cables2","gene_name":"Cables2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false}]},{"primerbank_id":"26326479a1","gene":"Mob3a","target_transcript":"NM_172457.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":1,"candidates":[{"rank":1,"left":"TTCCCCACTAATGTCGGCAC","right":"ATGCGGTCGAAATGGTGGAT","transcript_product_size_bp":116,"primer3_penalty":0.143,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":508,"end":623,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGACAAGACATTCAGGCCCA","right":"GATCCAGGCCCGCATTAAGA","transcript_product_size_bp":98,"primer3_penalty":0.1468,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527191,"end":80527288,"product_size":98,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":138,"end":235,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":329,"end":426,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGACAAGACATTCAGGCCCA","right":"CCAGGCCCGCATTAAGAGAT","transcript_product_size_bp":95,"primer3_penalty":0.1475,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527194,"end":80527288,"product_size":95,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":138,"end":232,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":329,"end":423,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGACAAGACATTCAGGCCCA","right":"ATCCAGGCCCGCATTAAGAG","transcript_product_size_bp":97,"primer3_penalty":0.1475,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527192,"end":80527288,"product_size":97,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":138,"end":234,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":329,"end":425,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGACAAGACATTCAGGCCCA","right":"AGATCCAGGCCCGCATTAAG","transcript_product_size_bp":99,"primer3_penalty":0.1475,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527190,"end":80527288,"product_size":99,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":138,"end":236,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":329,"end":427,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCCACCATCTTGCCACATTC","right":"TGGGCCTGAATGTCTTGTCC","transcript_product_size_bp":110,"primer3_penalty":0.2123,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527269,"end":80527378,"product_size":110,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":2,"right_hit_count":8,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":48,"end":157,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":239,"end":348,"product_size":110,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGACAAGACATTCAGGCCCA","right":"CAGATCCAGGCCCGCATTAA","transcript_product_size_bp":100,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527189,"end":80527288,"product_size":100,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":138,"end":237,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":329,"end":428,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCCACCATCTTGCCACATTC","right":"GATCCAGGCCCGCATTAAGA","transcript_product_size_bp":188,"primer3_penalty":0.2827,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527191,"end":80527378,"product_size":188,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":48,"end":235,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":239,"end":426,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCCACCATCTTGCCACATTC","right":"CCAGGCCCGCATTAAGAGAT","transcript_product_size_bp":185,"primer3_penalty":0.2835,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527194,"end":80527378,"product_size":185,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":48,"end":232,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":239,"end":423,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCCACCATCTTGCCACATTC","right":"ATCCAGGCCCGCATTAAGAG","transcript_product_size_bp":187,"primer3_penalty":0.2835,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_locus_accession":"NC_000076.7","target_locus_start":80521087,"target_locus_end":80537654,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":80527192,"end":80527378,"product_size":187,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172457.2","target_gene_id":"Mob3a","target_gene_name":"Mob3a","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172457.2","start":48,"end":234,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob3a","gene_name":"Mob3a"},{"transcript_accession":"XM_036155716.1","start":239,"end":425,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob3a","gene_name":"Mob3a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21717703a1","gene":"Vmn1r229","target_transcript":"NM_134190.1","candidate_pairs_screened":10,"combined_pass_pairs":9,"transcript_gene_specific_pairs":9,"transcript_isoform_specific_pairs":9,"candidates":[{"rank":1,"left":"CACTCTGTAGGCCGTGACAA","right":"TTCAGCAGAGGTCCTAGGGG","transcript_product_size_bp":183,"primer3_penalty":0.6428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035443,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":687,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CACTCTGTAGGCCGTGACAA","right":"GGGAAGCATGAGTGTGGAGG","transcript_product_size_bp":166,"primer3_penalty":0.7107,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035426,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":670,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CACTCTGTAGGCCGTGACAA","right":"GGGGAAGCATGAGTGTGGAG","transcript_product_size_bp":167,"primer3_penalty":0.7107,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035427,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":671,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CACTCTGTAGGCCGTGACAA","right":"GGAAGCATGAGTGTGGAGGA","transcript_product_size_bp":165,"primer3_penalty":0.9325,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035425,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":669,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CACTCTGTAGGCCGTGACAA","right":"AGGGGAAGCATGAGTGTGGA","transcript_product_size_bp":168,"primer3_penalty":1.1592,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035428,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":672,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AGCAAAGTTCATGGGCCTGT","right":"TTTGTCACGGCCTACAGAGT","transcript_product_size_bp":154,"primer3_penalty":1.2244,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035128,"end":21035281,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":372,"end":525,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACAGCAAAGTTCATGGGCCT","right":"TTTGTCACGGCCTACAGAGT","transcript_product_size_bp":156,"primer3_penalty":1.2244,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035126,"end":21035281,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":370,"end":525,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CACTCTGTAGGCCGTGACAA","right":"TAGGGGAAGCATGAGTGTGG","transcript_product_size_bp":169,"primer3_penalty":1.2289,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035261,"end":21035429,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":505,"end":673,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AAACAGAGCCACACAGACCA","right":"CGACTCATCACAAATGGGCC","transcript_product_size_bp":177,"primer3_penalty":1.281,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":7,"right_hit_count":3,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035443,"end":21035619,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000083.7","start":21133601,"end":21133777,"product_size":177,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000083.7","start":20996564,"end":20996740,"product_size":177,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":687,"end":863,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"},{"transcript_accession":"NM_134193.2","start":849,"end":1025,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r232","gene_name":"Vmn1r232"},{"transcript_accession":"NM_134192.3","start":1024,"end":1200,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r228","gene_name":"Vmn1r228"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"CAGCAAAGTTCATGGGCCTG","right":"TTTGTCACGGCCTACAGAGT","transcript_product_size_bp":155,"primer3_penalty":1.2909,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_locus_accession":"NC_000083.7","target_locus_start":21034757,"target_locus_end":21035677,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":21035127,"end":21035281,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134190.1","target_gene_id":"Vmn1r229","target_gene_name":"Vmn1r229","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134190.1","start":371,"end":525,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r229","gene_name":"Vmn1r229"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26338824a1","gene":"Rtp1","target_transcript":"NM_001004151.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GAGTCAGTTGGTGGGAAGGG","right":"GAAGGACTTCGGAACCCTGG","transcript_product_size_bp":185,"primer3_penalty":0.0732,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250441,"end":23250625,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":851,"end":1035,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGAGTCAGTTGGTGGGAAGG","right":"GAAGGACTTCGGAACCCTGG","transcript_product_size_bp":186,"primer3_penalty":0.0732,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250440,"end":23250625,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":850,"end":1035,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGGAGTCAGTTGGTGGGAAG","right":"GAAGGACTTCGGAACCCTGG","transcript_product_size_bp":187,"primer3_penalty":0.0732,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250439,"end":23250625,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":849,"end":1035,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GCGACCACCTACACCTTCTC","right":"CCCACCCCAAGCTTAGACAG","transcript_product_size_bp":171,"primer3_penalty":0.1438,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250270,"end":23250440,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":680,"end":850,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GTGCTTATTTTGGGCCACGG","right":"CCCACCCCAAGCTTAGACAG","transcript_product_size_bp":82,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250359,"end":23250440,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":769,"end":850,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGTGCTTATTTTGGGCCACG","right":"CCCACCCCAAGCTTAGACAG","transcript_product_size_bp":83,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250358,"end":23250440,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":768,"end":850,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCGACCACCTACACCTTCTC","right":"CTTCCCACCAACTGACTCCC","transcript_product_size_bp":189,"primer3_penalty":0.1456,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250270,"end":23250458,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":680,"end":868,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCGACCACCTACACCTTCTC","right":"CCTTCCCACCAACTGACTCC","transcript_product_size_bp":190,"primer3_penalty":0.1456,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250270,"end":23250459,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":680,"end":869,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCGACCACCTACACCTTCTC","right":"CCCTTCCCACCAACTGACTC","transcript_product_size_bp":191,"primer3_penalty":0.1456,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250270,"end":23250460,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":680,"end":870,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GTGCTTATTTTGGGCCACGG","right":"CTTCCCACCAACTGACTCCC","transcript_product_size_bp":100,"primer3_penalty":0.1457,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_locus_accession":"NC_000082.7","target_locus_start":23247883,"target_locus_end":23252710,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":23250359,"end":23250458,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001004151.2","target_gene_id":"Rtp1","target_gene_name":"Rtp1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001004151.2","start":769,"end":868,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Rtp1","gene_name":"Rtp1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31560689a1","gene":"Hmgcs2","target_transcript":"NM_008256.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"AGAAATCCCTGGCTCGGTTG","right":"AGACCCCTGAAGGCCTCTAG","transcript_product_size_bp":97,"primer3_penalty":0.0667,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":979,"end":1075,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CAGAAATCCCTGGCTCGGTT","right":"AGACCCCTGAAGGCCTCTAG","transcript_product_size_bp":98,"primer3_penalty":0.0667,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":978,"end":1075,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TCTTCCCTCTATGGCTGCCT","right":"AAGCATCCTTGGACACTCGG","transcript_product_size_bp":139,"primer3_penalty":0.0667,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98206569,"end":98208989,"product_size":2421,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":1203,"end":1341,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAAATCCCTGGCTCGGTTGA","right":"AGACCCCTGAAGGCCTCTAG","transcript_product_size_bp":96,"primer3_penalty":0.0671,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":980,"end":1075,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TCTTCCCTCTATGGCTGCCT","right":"GCATCCTTGGACACTCGGAA","transcript_product_size_bp":137,"primer3_penalty":0.0671,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98206569,"end":98208987,"product_size":2419,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":1203,"end":1339,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCTTCCCTCTATGGCTGCCT","right":"GACACCAGTTTCTCCAGGGG","transcript_product_size_bp":170,"primer3_penalty":0.0676,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":7,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98206569,"end":98209962,"product_size":3394,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":1203,"end":1372,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGAAATCCCTGGCTCGGTTG","right":"GGTAAAGGGAGGCCTTGGTC","transcript_product_size_bp":195,"primer3_penalty":0.0694,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98204769,"end":98206539,"product_size":1771,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":979,"end":1173,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGAAATCCCTGGCTCGGTT","right":"GGTAAAGGGAGGCCTTGGTC","transcript_product_size_bp":196,"primer3_penalty":0.0694,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98204768,"end":98206539,"product_size":1772,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":978,"end":1173,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GAAATCCCTGGCTCGGTTGA","right":"GGTAAAGGGAGGCCTTGGTC","transcript_product_size_bp":194,"primer3_penalty":0.0698,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":98204770,"end":98206539,"product_size":1770,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":980,"end":1173,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTCTCACCACTCTGCCCAAG","right":"AAGCATCCTTGGACACTCGG","transcript_product_size_bp":107,"primer3_penalty":0.0726,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_locus_accession":"NC_000069.7","target_locus_start":98187747,"target_locus_end":98218054,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_008256.4","target_gene_id":"Hmgcs2","target_gene_name":"Hmgcs2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_008256.4","start":1235,"end":1341,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcs2","gene_name":"Hmgcs2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13621162a1","gene":"Ide","target_transcript":"NM_031156.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GCTGATGACTGAAGTGGCCT","right":"CAATATGCAGCCGTGACAGC","transcript_product_size_bp":113,"primer3_penalty":0.0641,"exon_span":{"spans_junction":true,"left_exon":16,"right_exon":17,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37262637,"end":37264564,"product_size":1928,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":8,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2110,"end":2222,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2246,"end":2358,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":1974,"end":2086,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2149,"end":2261,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2210,"end":2322,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2008,"end":2120,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":3718,"end":3830,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GTGGACAGAAGGAAGGAGCC","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":141,"primer3_penalty":0.07,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37291450,"product_size":519,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1128,"end":1268,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1264,"end":1404,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":992,"end":1132,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1167,"end":1307,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1228,"end":1368,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":1026,"end":1166,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2736,"end":2876,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGTGGACAGAAGGAAGGAGC","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":142,"primer3_penalty":0.07,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37291451,"product_size":520,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1127,"end":1268,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1263,"end":1404,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":991,"end":1132,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1166,"end":1307,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1227,"end":1368,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":1025,"end":1166,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2735,"end":2876,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGTGCCCCTTGTTTGATGC","right":"GTTCCCCGTAGCCTTTTCCA","transcript_product_size_bp":123,"primer3_penalty":0.0751,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302578,"end":37302700,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":676,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":812,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":540,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":715,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":776,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":574,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2284,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACCAGACAGACATGCAGAGC","right":"GCGCAGGGTATTGAAGCAAG","transcript_product_size_bp":89,"primer3_penalty":0.136,"exon_span":{"spans_junction":false,"left_exon":19,"right_exon":19,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37258000,"end":37258088,"product_size":89,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2430,"end":2518,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2566,"end":2654,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":2294,"end":2382,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2469,"end":2557,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2530,"end":2618,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2328,"end":2416,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":4038,"end":4126,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTCCGCTTGCTGATGACTGA","right":"CAATATGCAGCCGTGACAGC","transcript_product_size_bp":121,"primer3_penalty":0.137,"exon_span":{"spans_junction":true,"left_exon":16,"right_exon":17,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37262637,"end":37264572,"product_size":1936,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2102,"end":2222,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2238,"end":2358,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":1966,"end":2086,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2141,"end":2261,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2202,"end":2322,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2000,"end":2120,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":3710,"end":3830,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGACAGGTTTGCGCAGTTTT","right":"GTTCCCCGTAGCCTTTTCCA","transcript_product_size_bp":145,"primer3_penalty":0.1395,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":532,"end":676,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":668,"end":812,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":396,"end":540,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":571,"end":715,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":632,"end":776,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":430,"end":574,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2140,"end":2284,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GTCCTGGAAGCCTGTTGTCA","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":198,"primer3_penalty":0.1426,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37292268,"product_size":1337,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1071,"end":1268,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1207,"end":1404,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":935,"end":1132,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1110,"end":1307,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1171,"end":1368,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":969,"end":1166,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2679,"end":2876,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGTGCCCCTTGTTTGATGC","right":"CTGAAGGGGTGTTTGGGGTT","transcript_product_size_bp":140,"primer3_penalty":0.1437,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302561,"end":37302700,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":693,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":829,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":557,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":732,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":793,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":591,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2301,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTGTGCCCCTTGTTTGATGC","right":"TGAAGGGGTGTTTGGGGTTC","transcript_product_size_bp":139,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302562,"end":37302700,"product_size":139,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":692,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":828,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":556,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":731,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":792,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":590,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2300,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6678003a1","gene":"Slc1a6","target_transcript":"NM_001428830.1","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":0,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"ACAATGGTTCAGAGCTGGGG","right":"GGCACAGTCTCCTCAAAGCT","transcript_product_size_bp":130,"primer3_penalty":0.0748,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":4,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78631810,"end":78631939,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":828,"end":957,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4013,"end":4142,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1235,"end":1364,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4013,"end":4142,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":760,"end":889,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":2,"left":"ACAATGGTTCAGAGCTGGGG","right":"AGGCACAGTCTCCTCAAAGC","transcript_product_size_bp":131,"primer3_penalty":0.0748,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":4,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78631810,"end":78631940,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":6,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":828,"end":958,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4013,"end":4143,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1235,"end":1365,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4013,"end":4143,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":760,"end":890,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":3,"left":"GCCGAATTGAGACTGTCCCA","right":"CCCCAGCTCTGAACCATTGT","transcript_product_size_bp":164,"primer3_penalty":0.0751,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":4,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78627155,"end":78631829,"product_size":4675,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":684,"end":847,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":3869,"end":4032,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1091,"end":1254,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":3869,"end":4032,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":616,"end":779,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":4,"left":"GCCGAATTGAGACTGTCCCA","right":"TTTGAAGCAGGCCTCCACAA","transcript_product_size_bp":89,"primer3_penalty":0.1427,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78627155,"end":78629163,"product_size":2009,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":9,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":684,"end":772,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":3869,"end":3957,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1091,"end":1179,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":3869,"end":3957,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":616,"end":704,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":5,"left":"TTGTGGAGGCCTGCTTCAAA","right":"CCCCAGCTCTGAACCATTGT","transcript_product_size_bp":95,"primer3_penalty":0.145,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78629144,"end":78631829,"product_size":2686,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":8,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":753,"end":847,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":3938,"end":4032,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1160,"end":1254,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":3938,"end":4032,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":685,"end":779,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":6,"left":"ACCTCTTCCAGCTCTGCAAC","right":"GTGCAGTGCCATCCATGTTG","transcript_product_size_bp":127,"primer3_penalty":0.145,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":7,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":1343,"end":1469,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4528,"end":4654,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1750,"end":1876,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4528,"end":4654,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":1275,"end":1401,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":7,"left":"CAACATGGATGGCACTGCAC","right":"ATGCTGCAAACGGGAAGAGA","transcript_product_size_bp":141,"primer3_penalty":0.1457,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_006513422.4","start":4635,"end":4775,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1857,"end":1997,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":1382,"end":1522,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":8,"left":"TCCTGGAAAATGTCACCCGG","right":"GGCCACAGAGAAGACCACAA","transcript_product_size_bp":125,"primer3_penalty":0.1462,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":4,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78631873,"end":78631997,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":891,"end":1015,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4076,"end":4200,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1298,"end":1422,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4076,"end":4200,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":823,"end":947,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":9,"left":"ACAATGGTTCAGAGCTGGGG","right":"GGCCACAGAGAAGACCACAA","transcript_product_size_bp":188,"primer3_penalty":0.1473,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":4,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":1,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":78631810,"end":78631997,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":828,"end":1015,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4013,"end":4200,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1235,"end":1422,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4013,"end":4200,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":760,"end":947,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false},{"rank":10,"left":"ACCTCTTCCAGCTCTGCAAC","right":"GCAGTGCCATCCATGTTGAC","transcript_product_size_bp":125,"primer3_penalty":0.2079,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_locus_accession":null,"target_locus_start":null,"target_locus_end":null,"target_locus_strand":null,"left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"target_not_found","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001428830.1","target_gene_id":null,"target_gene_name":null,"left_hit_count":6,"right_hit_count":8,"paired_amplicon_count":5,"target_transcript_amplicon_count":0,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009200.3","start":1343,"end":1467,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513422.4","start":4528,"end":4652,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_006513424.4","start":1750,"end":1874,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_030244972.1","start":4528,"end":4652,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"},{"transcript_accession":"XM_036155715.1","start":1275,"end":1399,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc1a6","gene_name":"Slc1a6"}],"message":"Transcript 'NM_001428830.1' was not present in the transcript locus database."},"combined_computational_pass":false}]},{"primerbank_id":"20126796a1","gene":"Dedd2","target_transcript":"NM_207677.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGATGGAGGAGGAAGGAGGG","right":"GAGGGTCGTCGTCTTTCTGG","transcript_product_size_bp":111,"primer3_penalty":0.1399,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":16,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902898,"end":24903008,"product_size":111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":12,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":1085,"end":1195,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1172,"end":1282,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1153,"end":1263,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1254,"end":1364,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1793,"end":1903,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1247,"end":1357,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCAGAAAGACGACGACCCTC","right":"TGCCTCAAAGCCCACATCAT","transcript_product_size_bp":113,"primer3_penalty":0.1493,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902805,"end":24902917,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":1176,"end":1288,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1263,"end":1375,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1244,"end":1356,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1345,"end":1457,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1884,"end":1996,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1338,"end":1450,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CCAGAAAGACGACGACCCTC","right":"ATGCCTCAAAGCCCACATCA","transcript_product_size_bp":114,"primer3_penalty":0.1493,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902804,"end":24902917,"product_size":114,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":1176,"end":1289,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1263,"end":1376,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1244,"end":1357,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1345,"end":1458,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1884,"end":1997,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1338,"end":1451,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGCTGATGGAGGAGGAAGGA","right":"GAGGGTCGTCGTCTTTCTGG","transcript_product_size_bp":114,"primer3_penalty":0.1524,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902898,"end":24903011,"product_size":114,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":12,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":1082,"end":1195,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1169,"end":1282,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1150,"end":1263,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1251,"end":1364,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1790,"end":1903,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1244,"end":1357,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GACTGAGGCTTTACGGGAGG","right":"CCCTCCTTCCTCCTCCATCA","transcript_product_size_bp":118,"primer3_penalty":0.2067,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":15,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902989,"end":24903106,"product_size":118,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":10,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":987,"end":1104,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1074,"end":1191,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1055,"end":1172,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1156,"end":1273,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1695,"end":1812,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1149,"end":1266,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GACTGAGGCTTTACGGGAGG","right":"CAGCCTCATAGTCAGCCTCG","transcript_product_size_bp":80,"primer3_penalty":0.2078,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24903027,"end":24903106,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":987,"end":1066,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1074,"end":1153,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1055,"end":1134,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1156,"end":1235,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1695,"end":1774,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1149,"end":1228,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTGTTGCTGATGGAGGAGG","right":"GAGGGTCGTCGTCTTTCTGG","transcript_product_size_bp":119,"primer3_penalty":0.2165,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902898,"end":24903016,"product_size":119,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":1077,"end":1195,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1164,"end":1282,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1145,"end":1263,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1246,"end":1364,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1785,"end":1903,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1239,"end":1357,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GACTGAGGCTTTACGGGAGG","right":"TCCTTCCTCCTCCATCAGCA","transcript_product_size_bp":115,"primer3_penalty":0.2192,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24902992,"end":24903106,"product_size":115,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":12,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":987,"end":1101,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":1074,"end":1188,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":1055,"end":1169,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1156,"end":1270,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1695,"end":1809,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1149,"end":1263,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGCTCTGTGGTGTGTGACAT","right":"GCCTCATAGTCAGCCTCGTC","transcript_product_size_bp":185,"primer3_penalty":0.2819,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24903029,"end":24903213,"product_size":185,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":880,"end":1064,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":967,"end":1151,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":948,"end":1132,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1049,"end":1233,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1588,"end":1772,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1042,"end":1226,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GGCTCTGTGGTGTGTGACAT","right":"CAGCCTCATAGTCAGCCTCG","transcript_product_size_bp":187,"primer3_penalty":0.2823,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_locus_accession":"NC_000073.7","target_locus_start":24902265,"target_locus_end":24919284,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":24903027,"end":24903213,"product_size":187,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207677.3","target_gene_id":"Dedd2","target_gene_name":"Dedd2","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207677.3","start":880,"end":1066,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_030242925.2","start":967,"end":1153,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XM_036153381.1","start":948,"end":1134,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_003946545.2","start":1049,"end":1235,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_004934172.1","start":1588,"end":1774,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"},{"transcript_accession":"XR_391094.3","start":1042,"end":1228,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Dedd2","gene_name":"Dedd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"6677733a1","gene":"Slc50a1","target_transcript":"NM_001410551.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GCTAGGCCTCTTCTGTAGCG","right":"AAGCTGGTGAGGATTCCGTG","transcript_product_size_bp":197,"primer3_penalty":0.0675,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":9,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":459,"end":655,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":329,"end":525,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":374,"end":570,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":705,"end":901,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GCTAGGCCTCTTCTGTAGCG","right":"GTGGCAATGGTCAGGGAGAA","transcript_product_size_bp":120,"primer3_penalty":0.0696,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89176041,"end":89176417,"product_size":377,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":9,"right_hit_count":12,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":459,"end":578,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410552.1","start":329,"end":448,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410553.1","start":374,"end":493,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410554.1","start":705,"end":824,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":329,"end":448,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":374,"end":493,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":705,"end":824,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_009057.4","start":459,"end":578,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"XR_004941298.1","start":577,"end":696,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCTAGGCCTCTTCTGTAGCG","right":"TGGTCAGGGAGAAGGAGAGG","transcript_product_size_bp":113,"primer3_penalty":0.0733,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89176048,"end":89176417,"product_size":370,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":9,"right_hit_count":12,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":459,"end":571,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410552.1","start":329,"end":441,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410553.1","start":374,"end":486,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410554.1","start":705,"end":817,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":329,"end":441,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":374,"end":486,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":705,"end":817,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_009057.4","start":459,"end":571,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"XR_004941298.1","start":577,"end":689,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTCTCCCTGACCATTGCCAC","right":"AAGCTGGTGAGGATTCCGTG","transcript_product_size_bp":97,"primer3_penalty":0.0742,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":559,"end":655,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":429,"end":525,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":474,"end":570,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":805,"end":901,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":397,"end":493,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CATTGCCACGCTCTTTTGCT","right":"AAGCTGGTGAGGATTCCGTG","transcript_product_size_bp":86,"primer3_penalty":0.0749,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":570,"end":655,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":440,"end":525,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":485,"end":570,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":816,"end":901,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":408,"end":493,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TTCTCCCTGACCATTGCCAC","right":"GCGGTACTTCCTGTCTTGCT","transcript_product_size_bp":152,"primer3_penalty":0.0754,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89175819,"end":89176060,"product_size":242,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":12,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":559,"end":710,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":429,"end":580,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":474,"end":625,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":805,"end":956,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":397,"end":548,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410552.1","start":429,"end":596,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410553.1","start":474,"end":641,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410554.1","start":805,"end":972,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410555.1","start":397,"end":564,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410560.1","start":643,"end":810,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_009057.4","start":559,"end":726,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"XR_004941298.1","start":677,"end":844,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CATTGCCACGCTCTTTTGCT","right":"GCGGTACTTCCTGTCTTGCT","transcript_product_size_bp":141,"primer3_penalty":0.0761,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89175819,"end":89176049,"product_size":231,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":12,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":570,"end":710,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":440,"end":580,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":485,"end":625,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":816,"end":956,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":408,"end":548,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410552.1","start":440,"end":596,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410553.1","start":485,"end":641,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410554.1","start":816,"end":972,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410555.1","start":408,"end":564,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410560.1","start":654,"end":810,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_009057.4","start":570,"end":726,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"XR_004941298.1","start":688,"end":844,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCTCTCCTTCTCCCTGACCA","right":"AAGCTGGTGAGGATTCCGTG","transcript_product_size_bp":104,"primer3_penalty":0.0778,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":552,"end":655,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":422,"end":525,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":467,"end":570,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":798,"end":901,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":390,"end":493,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCTCTCCTTCTCCCTGACCA","right":"GCGGTACTTCCTGTCTTGCT","transcript_product_size_bp":159,"primer3_penalty":0.0791,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":89175819,"end":89176067,"product_size":249,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":12,"right_hit_count":12,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":552,"end":710,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":422,"end":580,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":467,"end":625,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":798,"end":956,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410559.1","start":390,"end":548,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410552.1","start":422,"end":596,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410553.1","start":467,"end":641,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410554.1","start":798,"end":972,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410555.1","start":390,"end":564,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410560.1","start":636,"end":810,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_009057.4","start":552,"end":726,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"XR_004941298.1","start":670,"end":844,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AGCATGTACCTCTCCCCACT","right":"AAGCTGGTGAGGATTCCGTG","transcript_product_size_bp":166,"primer3_penalty":0.0793,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_locus_accession":"NC_000069.7","target_locus_start":89175553,"target_locus_end":89177847,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001410551.1","target_gene_id":"Slc50a1","target_gene_name":"Slc50a1","left_hit_count":9,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001410551.1","start":490,"end":655,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410556.1","start":360,"end":525,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410557.1","start":405,"end":570,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"},{"transcript_accession":"NM_001410558.1","start":736,"end":901,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc50a1","gene_name":"Slc50a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31127154a1","gene":"Slc16a10","target_transcript":"NM_001114332.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AGCGCCTTGGACTAGTCAAC","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":121,"primer3_penalty":0.074,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952912,"product_size":121,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":799,"end":919,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":799,"end":919,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":278,"end":398,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":811,"end":931,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACGACGTGGCCTTTTACCTT","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":129,"primer3_penalty":0.2132,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913373,"product_size":129,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1552,"end":1680,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1031,"end":1159,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1596,"end":1724,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCAGCAGCGTCTTCACAATC","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":85,"primer3_penalty":0.216,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952876,"product_size":85,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":835,"end":919,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":835,"end":919,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":314,"end":398,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":847,"end":931,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACTTCATGACAAGCTGGGCA","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":152,"primer3_penalty":0.217,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913396,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1529,"end":1680,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1008,"end":1159,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1573,"end":1724,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCCGTCACTGGTCATTTTGG","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":156,"primer3_penalty":0.2777,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952947,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":764,"end":919,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":764,"end":919,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":243,"end":398,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":776,"end":931,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GAAGCGCCTTGGACTAGTCA","right":"AGGATCCGCAGCGTGTTAAA","transcript_product_size_bp":123,"primer3_penalty":0.2834,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39952792,"end":39952914,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":797,"end":919,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"NM_028247.4","start":797,"end":919,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":276,"end":398,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":809,"end":931,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACGTGGCCTTTTACCTTGCT","right":"TCTCCATCTTCTCCCCTCCG","transcript_product_size_bp":126,"primer3_penalty":0.285,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913245,"end":39913370,"product_size":126,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":15,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1555,"end":1680,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":1034,"end":1159,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1599,"end":1724,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TTCAGGAGTTGGACGGCTTC","right":"TCGAAGAGACCCATGGCAAG","transcript_product_size_bp":174,"primer3_penalty":0.2851,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1223,"end":1396,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":702,"end":875,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1267,"end":1440,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTTCAGGAGTTGGACGGCTT","right":"TCGAAGAGACCCATGGCAAG","transcript_product_size_bp":175,"primer3_penalty":0.2857,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1222,"end":1396,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":701,"end":875,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1266,"end":1440,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGCCATGGGTCTCTTCGATG","right":"AAGGTAAAAGGCCACGTCGT","transcript_product_size_bp":193,"primer3_penalty":0.286,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_locus_accession":"NC_000076.7","target_locus_start":39909531,"target_locus_end":40018250,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":39913354,"end":39916740,"product_size":3387,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001114332.1","target_gene_id":"Slc16a10","target_gene_name":"Slc16a10","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001114332.1","start":1379,"end":1571,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XM_006512863.4","start":858,"end":1050,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"},{"transcript_accession":"XR_380076.5","start":1423,"end":1615,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc16a10","gene_name":"Slc16a10"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"12857247a1","gene":"Dcp2","target_transcript":"NM_027490.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"AGAAGCTGCATCCACGGAAA","right":"GACGGCTCTTCACCAGAACA","transcript_product_size_bp":82,"primer3_penalty":0.0702,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548454,"end":44550672,"product_size":2219,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1198,"end":1279,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AAGAAGCTGCATCCACGGAA","right":"GACGGCTCTTCACCAGAACA","transcript_product_size_bp":83,"primer3_penalty":0.0702,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548453,"end":44550672,"product_size":2220,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1197,"end":1279,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AAAGAAGCTGCATCCACGGA","right":"GACGGCTCTTCACCAGAACA","transcript_product_size_bp":84,"primer3_penalty":0.0702,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548452,"end":44550672,"product_size":2221,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1196,"end":1279,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAAGCTGCATCCACGGAAAC","right":"GACGGCTCTTCACCAGAACA","transcript_product_size_bp":81,"primer3_penalty":0.2025,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548455,"end":44550672,"product_size":2218,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1199,"end":1279,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGCAGCAGAAGTCACACAGT","right":"TCCGTGGATGCAGCTTCTTT","transcript_product_size_bp":195,"primer3_penalty":0.2155,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1021,"end":1215,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCAGCAGAAGTCACACAGT","right":"TTCCGTGGATGCAGCTTCTT","transcript_product_size_bp":196,"primer3_penalty":0.2155,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1021,"end":1216,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGCAGCAGAAGTCACACAGT","right":"TTTCCGTGGATGCAGCTTCT","transcript_product_size_bp":197,"primer3_penalty":0.2155,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1021,"end":1217,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGAAGCTGCATCCACGGAAA","right":"TGGGAACTTGCAATGTCCGT","transcript_product_size_bp":137,"primer3_penalty":0.2157,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548454,"end":44550727,"product_size":2274,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1198,"end":1334,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AAGAAGCTGCATCCACGGAA","right":"TGGGAACTTGCAATGTCCGT","transcript_product_size_bp":138,"primer3_penalty":0.2157,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548453,"end":44550727,"product_size":2275,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1197,"end":1334,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AAAGAAGCTGCATCCACGGA","right":"TGGGAACTTGCAATGTCCGT","transcript_product_size_bp":139,"primer3_penalty":0.2157,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_locus_accession":"NC_000084.7","target_locus_start":44513567,"target_locus_end":44558036,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000084.7","start":44548452,"end":44550727,"product_size":2276,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027490.1","target_gene_id":"Dcp2","target_gene_name":"Dcp2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027490.1","start":1196,"end":1334,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Dcp2","gene_name":"Dcp2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26339622a1","gene":"Slco5a1","target_transcript":"XM_011238381.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TCGCTGCTTCTTAGTGTGCA","right":"TAGCTGACGAACACCACCAC","transcript_product_size_bp":180,"primer3_penalty":0.0663,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060151,"end":13060330,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1098,"end":1277,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1030,"end":1209,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1103,"end":1282,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1150,"end":1329,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1150,"end":1329,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2360,"end":2539,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1103,"end":1282,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1082,"end":1261,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1075,"end":1254,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCGCTGCTTCTTAGTGTGCA","right":"GTAGCTGACGAACACCACCA","transcript_product_size_bp":181,"primer3_penalty":0.0663,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060150,"end":13060330,"product_size":181,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1098,"end":1278,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1030,"end":1210,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1103,"end":1283,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1150,"end":1330,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1150,"end":1330,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2360,"end":2540,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1103,"end":1283,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1082,"end":1262,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1075,"end":1255,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGTGGGCTACTCATTGGCTT","right":"TCTTGTGTCGAGGTGGAAGC","transcript_product_size_bp":175,"primer3_penalty":0.0676,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13009333,"end":13014286,"product_size":4954,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":11,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1675,"end":1849,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1680,"end":1781,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1652,"end":1753,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1607,"end":1781,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1727,"end":1901,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1727,"end":1901,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2937,"end":3111,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCTTACGGATTCTCGCTGCT","right":"TAGCTGACGAACACCACCAC","transcript_product_size_bp":192,"primer3_penalty":0.1362,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060151,"end":13060342,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1086,"end":1277,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1018,"end":1209,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1091,"end":1282,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1138,"end":1329,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1138,"end":1329,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2348,"end":2539,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1091,"end":1282,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1070,"end":1261,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1063,"end":1254,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCTTACGGATTCTCGCTGCT","right":"GTAGCTGACGAACACCACCA","transcript_product_size_bp":193,"primer3_penalty":0.1362,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060150,"end":13060342,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1086,"end":1278,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1018,"end":1210,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1091,"end":1283,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1138,"end":1330,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1138,"end":1330,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2348,"end":2540,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1091,"end":1283,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1070,"end":1262,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1063,"end":1255,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ACCTTGACCAGAGTGACCCT","right":"TCTTGTGTCGAGGTGGAAGC","transcript_product_size_bp":129,"primer3_penalty":0.1384,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13009333,"end":13014240,"product_size":4908,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":11,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1721,"end":1849,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1726,"end":1781,"product_size":56,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1698,"end":1753,"product_size":56,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1653,"end":1781,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1773,"end":1901,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1773,"end":1901,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2983,"end":3111,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGCTGGGAGTACAACGTGAC","right":"CCCACTTGCTCACTCACAGT","transcript_product_size_bp":179,"primer3_penalty":0.1404,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12942288,"end":12942466,"product_size":179,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2887,"end":3065,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2819,"end":2997,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2819,"end":2997,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2939,"end":3117,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2774,"end":2952,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3984,"end":4162,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":2680,"end":2858,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":2659,"end":2837,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2791,"end":2969,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCGATTTACACCCTGGGACC","right":"AAGCCAATGAGTAGCCCACC","transcript_product_size_bp":140,"primer3_penalty":0.1415,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1555,"end":1694,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1487,"end":1626,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1560,"end":1699,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1607,"end":1746,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1607,"end":1746,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2817,"end":2956,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1560,"end":1699,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1539,"end":1678,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1532,"end":1671,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TCATCATTGTCCCCAGTGCC","right":"GAACAGGGTGGAGAAGCACA","transcript_product_size_bp":140,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12964707,"end":12964846,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":14,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2138,"end":2277,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2070,"end":2209,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2070,"end":2209,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2190,"end":2329,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2025,"end":2164,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3235,"end":3374,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1931,"end":2070,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1910,"end":2049,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2042,"end":2181,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCATCATTGTCCCCAGTGCC","right":"TGAACAGGGTGGAGAAGCAC","transcript_product_size_bp":141,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12964706,"end":12964846,"product_size":141,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2138,"end":2278,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2070,"end":2210,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2070,"end":2210,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2190,"end":2330,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2025,"end":2165,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3235,"end":3375,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1931,"end":2071,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1910,"end":2050,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2042,"end":2182,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"34328334a1","gene":"Acox3","target_transcript":"NM_030721.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TACCGCTGGCTTGTTTGCTA","right":"GTTCGTGGAATCGCTGCATC","transcript_product_size_bp":169,"primer3_penalty":0.0624,"exon_span":{"spans_junction":true,"left_exon":14,"right_exon":15,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35764440,"end":35765668,"product_size":1229,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1733,"end":1901,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":1918,"end":2086,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1729,"end":1897,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2195,"end":2363,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":1914,"end":2082,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1763,"end":1931,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2010,"end":2178,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":1849,"end":2017,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":1849,"end":2017,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2274,"end":2442,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":1849,"end":2017,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1655,"end":1823,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GCTCACTTCACAAGCCCTCT","right":"TCTCCGATGGCTCTCTTGGA","transcript_product_size_bp":168,"primer3_penalty":0.0691,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35762556,"end":35764484,"product_size":1929,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1610,"end":1777,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":1795,"end":1962,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1606,"end":1773,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2072,"end":2239,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":1791,"end":1958,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1640,"end":1807,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":1887,"end":2054,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":1726,"end":1893,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":1726,"end":1893,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2151,"end":2318,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":1726,"end":1893,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1532,"end":1699,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCCTATGCCTTGGACCACTT","right":"CTCACGGCACTCCTGGATAC","transcript_product_size_bp":186,"primer3_penalty":0.1373,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":11,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35758877,"end":35760357,"product_size":1481,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":18,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1268,"end":1453,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":1453,"end":1638,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1264,"end":1449,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":1730,"end":1915,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":1449,"end":1634,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1298,"end":1483,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":1545,"end":1730,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":1384,"end":1569,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":1384,"end":1569,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":1809,"end":1994,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":1384,"end":1569,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1190,"end":1375,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCTGTCTCCATCTCTGCGGA","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":136,"primer3_penalty":0.138,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765685,"end":35766407,"product_size":723,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1918,"end":2053,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2103,"end":2238,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1914,"end":2049,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2380,"end":2515,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2099,"end":2234,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1948,"end":2083,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2195,"end":2330,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2034,"end":2169,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2034,"end":2169,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2459,"end":2594,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2034,"end":2169,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1840,"end":1975,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCGACAGGCTAAAGTCCCAG","right":"CTTGGAGCTCTGTGTCTGGG","transcript_product_size_bp":97,"primer3_penalty":0.1447,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35769453,"end":35769549,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":2259,"end":2355,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2444,"end":2540,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":2255,"end":2351,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2440,"end":2536,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165627.1","start":4369,"end":4465,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165628.1","start":2181,"end":2277,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTCTCCATCTCTGCGGACTG","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":133,"primer3_penalty":0.2061,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765688,"end":35766407,"product_size":720,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1921,"end":2053,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2106,"end":2238,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1917,"end":2049,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2383,"end":2515,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2102,"end":2234,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1951,"end":2083,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2198,"end":2330,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2037,"end":2169,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2037,"end":2169,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2462,"end":2594,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2037,"end":2169,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1843,"end":1975,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CTGTCTCCATCTCTGCGGAC","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":135,"primer3_penalty":0.2061,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765686,"end":35766407,"product_size":722,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":16,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1919,"end":2053,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2104,"end":2238,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1915,"end":2049,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2381,"end":2515,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2100,"end":2234,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1949,"end":2083,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2196,"end":2330,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2035,"end":2169,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2035,"end":2169,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2460,"end":2594,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2035,"end":2169,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1841,"end":1975,"product_size":135,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GTCTGTCTCCATCTCTGCGG","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":137,"primer3_penalty":0.2061,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765684,"end":35766407,"product_size":724,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1917,"end":2053,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2102,"end":2238,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1913,"end":2049,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2379,"end":2515,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2098,"end":2234,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1947,"end":2083,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2194,"end":2330,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2033,"end":2169,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2033,"end":2169,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2458,"end":2594,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2033,"end":2169,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1839,"end":1975,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGTCTGTCTCCATCTCTGCG","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":138,"primer3_penalty":0.2061,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765683,"end":35766407,"product_size":725,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1916,"end":2053,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2101,"end":2238,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1912,"end":2049,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2378,"end":2515,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2097,"end":2234,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1946,"end":2083,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2193,"end":2330,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2032,"end":2169,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2032,"end":2169,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2457,"end":2594,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2032,"end":2169,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1838,"end":1975,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CGGTCTGTCTCCATCTCTGC","right":"GTCCTCCATCGCTCTTCCTG","transcript_product_size_bp":139,"primer3_penalty":0.2061,"exon_span":{"spans_junction":true,"left_exon":15,"right_exon":16,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_locus_accession":"NC_000071.7","target_locus_start":35740409,"target_locus_end":35771145,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":35765682,"end":35766407,"product_size":726,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_030721.4","target_gene_id":"Acox3","target_gene_name":"Acox3","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_030721.4","start":1915,"end":2053,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357018.2","start":2100,"end":2238,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357019.2","start":1911,"end":2049,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001357020.2","start":2377,"end":2515,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"NM_001425797.1","start":2096,"end":2234,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504196.5","start":1945,"end":2083,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504197.5","start":2192,"end":2330,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504199.5","start":2031,"end":2169,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_006504200.5","start":2031,"end":2169,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_011240801.4","start":2456,"end":2594,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_017321179.3","start":2031,"end":2169,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"},{"transcript_accession":"XM_036165626.1","start":1837,"end":1975,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acox3","gene_name":"Acox3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"12848777a1","gene":"Eif2s3y","target_transcript":"NM_012011.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TTGACCCAACGTTATGCCGA","right":"TACGTACACCTAGGAGCCGT","transcript_product_size_bp":129,"primer3_penalty":0.2881,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020001,"end":1020129,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1050,"end":1178,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":955,"end":1083,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1369,"end":1497,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1077,"end":1205,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":988,"end":1116,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1077,"end":1205,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1580,"end":1708,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1450,"end":1578,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1189,"end":1317,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1182,"end":1310,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1587,"end":1715,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1450,"end":1578,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACTTCTGAACACCTGGCTGC","right":"GCTCCTTCGGCTACTGTACC","transcript_product_size_bp":149,"primer3_penalty":0.6387,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1014663,"end":1016075,"product_size":1413,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":18,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":530,"end":678,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":435,"end":583,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":849,"end":997,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":468,"end":616,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782887.3","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":557,"end":705,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGACCCAACGTTATGCCGAG","right":"TACGTACACCTAGGAGCCGT","transcript_product_size_bp":128,"primer3_penalty":0.6436,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020002,"end":1020129,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1051,"end":1178,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":956,"end":1083,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1370,"end":1497,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1078,"end":1205,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":989,"end":1116,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1078,"end":1205,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1581,"end":1708,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1451,"end":1578,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1190,"end":1317,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1183,"end":1310,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1588,"end":1715,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1451,"end":1578,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTGACCCAACGTTATGCCGA","right":"GTACGTACACCTAGGAGCCG","transcript_product_size_bp":130,"primer3_penalty":0.695,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020001,"end":1020130,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1050,"end":1179,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":955,"end":1084,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1369,"end":1498,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1077,"end":1206,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":988,"end":1117,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1077,"end":1206,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1580,"end":1709,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1450,"end":1579,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1189,"end":1318,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1182,"end":1311,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1587,"end":1716,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1450,"end":1579,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TTGACCCAACGTTATGCCGA","right":"CGTACACCTAGGAGCCGTCT","transcript_product_size_bp":127,"primer3_penalty":0.7823,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020001,"end":1020127,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":17,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1050,"end":1176,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":955,"end":1081,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1369,"end":1495,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1077,"end":1203,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":988,"end":1114,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1077,"end":1203,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1580,"end":1706,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1450,"end":1576,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1189,"end":1315,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1182,"end":1308,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1587,"end":1713,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1450,"end":1576,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ACGGCTCCTAGGTGTACGTA","right":"CCCAAATCTGCCTTGACTGC","transcript_product_size_bp":138,"primer3_penalty":0.7824,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020110,"end":1023393,"product_size":3284,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":18,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1159,"end":1296,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":1064,"end":1201,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1478,"end":1615,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1186,"end":1323,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":1097,"end":1234,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1186,"end":1323,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1689,"end":1826,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1559,"end":1696,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1298,"end":1435,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1291,"end":1428,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1696,"end":1833,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1559,"end":1696,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACGGCTCCTAGGTGTACGTA","right":"GCCTCCTGTAGACAAGGACC","transcript_product_size_bp":109,"primer3_penalty":0.7907,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020110,"end":1023364,"product_size":3255,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":17,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1159,"end":1267,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":1064,"end":1172,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1478,"end":1586,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1186,"end":1294,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":1097,"end":1205,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1186,"end":1294,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1689,"end":1797,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1559,"end":1667,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1298,"end":1406,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1291,"end":1399,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1696,"end":1804,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1559,"end":1667,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ACGGCTCCTAGGTGTACGTA","right":"GCACTAACTCTGCCTCCTGT","transcript_product_size_bp":120,"primer3_penalty":0.8637,"exon_span":{"spans_junction":true,"left_exon":9,"right_exon":10,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020110,"end":1023375,"product_size":3266,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":18,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1159,"end":1278,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":1064,"end":1183,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1478,"end":1597,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1186,"end":1305,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":1097,"end":1216,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1186,"end":1305,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1689,"end":1808,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1559,"end":1678,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1298,"end":1417,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1291,"end":1410,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1696,"end":1815,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1559,"end":1678,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ACTTCTGAACACCTGGCTGC","right":"TCCTTCGGCTACTGTACCCT","transcript_product_size_bp":147,"primer3_penalty":0.87,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":18,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":16,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":530,"end":676,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":435,"end":581,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":849,"end":995,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":468,"end":614,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782887.3","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":557,"end":703,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TAGAATGGTTGGGCAGGTCC","right":"TACGTACACCTAGGAGCCGT","transcript_product_size_bp":104,"primer3_penalty":0.8768,"exon_span":{"spans_junction":false,"left_exon":9,"right_exon":9,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_locus_accession":"NC_000087.8","target_locus_start":1010595,"target_locus_end":1028594,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000087.8","start":1020026,"end":1020129,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_012011.2","target_gene_id":"Eif2s3y","target_gene_name":"Eif2s3y","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_012011.2","start":1075,"end":1178,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"NM_001357094.1","start":980,"end":1083,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_006531610.4","start":1394,"end":1497,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_011248593.3","start":1102,"end":1205,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_030251569.2","start":1013,"end":1116,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XM_036162192.1","start":1102,"end":1205,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782883.2","start":1605,"end":1708,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782884.2","start":1475,"end":1578,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782885.2","start":1214,"end":1317,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_001782886.2","start":1207,"end":1310,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_003953252.1","start":1612,"end":1715,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"},{"transcript_accession":"XR_004940598.1","start":1475,"end":1578,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Eif2s3y","gene_name":"Eif2s3y"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"27370472a1","gene":"Adck5","target_transcript":"NM_172960.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AATGAGGGCCGAAATGCTGA","right":"TGCCCTCCATGTCATTCACC","transcript_product_size_bp":148,"primer3_penalty":0.0684,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":76478392,"end":76478618,"product_size":227,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":962,"end":1109,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":957,"end":1104,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGTGAATGACATGGAGGGCA","right":"ACCAGCTCTGCCTTTCCATC","transcript_product_size_bp":171,"primer3_penalty":0.0689,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":10,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":76478599,"end":76478913,"product_size":315,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":4,"right_hit_count":7,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":1090,"end":1260,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":1085,"end":1255,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AAACCGGGAAGAGAGCACTG","right":"CCTGGCTCTTGAGGTCCTTG","transcript_product_size_bp":160,"primer3_penalty":0.0711,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":97,"end":256,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":97,"end":256,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GCATTGCCTACTCTTTCGCG","right":"GCAGCAGGGTAGAGTGGAAG","transcript_product_size_bp":126,"primer3_penalty":0.1347,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":45,"end":170,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":45,"end":170,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CGCATTGCCTACTCTTTCGC","right":"GCAGCAGGGTAGAGTGGAAG","transcript_product_size_bp":127,"primer3_penalty":0.1347,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":44,"end":170,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":44,"end":170,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CATGGCCCGTGAACACTTTG","right":"GAGGAAGTAGCGGTCGACAG","transcript_product_size_bp":139,"primer3_penalty":0.1383,"exon_span":{"spans_junction":false,"left_exon":12,"right_exon":12,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":76479317,"end":76479455,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":1495,"end":1633,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":1490,"end":1628,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CTTCCACTCTACCCTGCTGC","right":"CCTGGCTCTTGAGGTCCTTG","transcript_product_size_bp":106,"primer3_penalty":0.1436,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":151,"end":256,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":151,"end":256,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGTGAATGACATGGAGGGCA","right":"ACCTTTCCGCACCAGAACAT","transcript_product_size_bp":148,"primer3_penalty":0.1447,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":10,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":1090,"end":1237,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":1085,"end":1232,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGTGCTCCTTCAACCACCT","right":"GGGAGTGCCTGGAAGTCTTC","transcript_product_size_bp":128,"primer3_penalty":0.1453,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000081.7","start":76477742,"end":76477956,"product_size":215,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":578,"end":705,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":573,"end":700,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CATGGCCCGTGAACACTTTG","right":"AACCCCAGACAGCACTCTTG","transcript_product_size_bp":164,"primer3_penalty":0.1486,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_locus_accession":"NC_000081.7","target_locus_start":76460559,"target_locus_end":76480012,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172960.3","target_gene_id":"Adck5","target_gene_name":"Adck5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172960.3","start":1495,"end":1658,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck5","gene_name":"Adck5"},{"transcript_accession":"NR_073123.1","start":1490,"end":1653,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck5","gene_name":"Adck5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13384718a1","gene":"Ube2d3","target_transcript":"NM_001356597.1","candidate_pairs_screened":10,"combined_pass_pairs":0,"transcript_gene_specific_pairs":0,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CAGAGCCGGTGTGAGAAGAA","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":166,"primer3_penalty":0.3543,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":19,"paired_amplicon_count":6,"target_amplicon_count":0,"off_target_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398755,"end":23398920,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322342,"product_size":164,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538380,"end":73538545,"product_size":166,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000071.7","start":31514083,"end":31515011,"product_size":929,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000074.7","start":27313173,"end":27313338,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000071.7","start":22928950,"end":22932074,"product_size":3125,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":533,"end":698,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":136,"end":301,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":132,"end":297,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":234,"end":399,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":179,"end":344,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":179,"end":344,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":500,"end":665,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":268,"end":433,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":131,"end":296,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":180,"end":345,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":181,"end":346,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3967,"product_size":166,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"CAGAGCCGGTGTGAGAAGAA","right":"ATCCGTTTCAGCGCCATAGT","transcript_product_size_bp":134,"primer3_penalty":0.4965,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":9,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":135145419,"end":135145552,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000068.8","start":23398755,"end":23398888,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322211,"end":116322342,"product_size":132,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538412,"end":73538545,"product_size":134,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000074.7","start":27313173,"end":27313306,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":533,"end":666,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":136,"end":269,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":132,"end":265,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":234,"end":367,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":179,"end":312,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":179,"end":312,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":500,"end":633,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":268,"end":401,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":131,"end":264,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":180,"end":313,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":181,"end":314,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3834,"end":3967,"product_size":134,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"CAGAGCCGGTGTGAGAAGAA","right":"ACATTGTGCTGGAGGGTCAC","transcript_product_size_bp":180,"primer3_penalty":0.5693,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":27,"paired_amplicon_count":3,"target_amplicon_count":0,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398755,"end":23398934,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000074.7","start":27313173,"end":27313352,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538366,"end":73538545,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":533,"end":712,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":136,"end":315,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":132,"end":311,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":234,"end":413,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":179,"end":358,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":179,"end":358,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":500,"end":679,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":268,"end":447,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":131,"end":310,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":180,"end":359,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":181,"end":360,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3788,"end":3967,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":4,"left":"GCCGGTGTGAGAAGAAGAGA","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":162,"primer3_penalty":0.6394,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":19,"paired_amplicon_count":4,"target_amplicon_count":0,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398759,"end":23398920,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322338,"product_size":160,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000071.7","start":31514083,"end":31515007,"product_size":925,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000074.7","start":27313177,"end":27313338,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":537,"end":698,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":140,"end":301,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":136,"end":297,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":238,"end":399,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":183,"end":344,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":183,"end":344,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":504,"end":665,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":272,"end":433,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":135,"end":296,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":184,"end":345,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":185,"end":346,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3963,"product_size":162,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"GAGCCGGTGTGAGAAGAAGA","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":164,"primer3_penalty":0.6394,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":7,"right_hit_count":19,"paired_amplicon_count":5,"target_amplicon_count":0,"off_target_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398757,"end":23398920,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322340,"product_size":162,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538380,"end":73538543,"product_size":164,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000071.7","start":31514083,"end":31515009,"product_size":927,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000074.7","start":27313175,"end":27313338,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":535,"end":698,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":138,"end":301,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":134,"end":297,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":236,"end":399,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":181,"end":344,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":181,"end":344,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":502,"end":665,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":270,"end":433,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":133,"end":296,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":182,"end":345,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":183,"end":346,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3965,"product_size":164,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"AGCCGGTGTGAGAAGAAGAG","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":163,"primer3_penalty":0.6414,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":19,"paired_amplicon_count":5,"target_amplicon_count":0,"off_target_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398758,"end":23398920,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322339,"product_size":161,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538380,"end":73538542,"product_size":163,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000071.7","start":31514083,"end":31515008,"product_size":926,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000074.7","start":27313176,"end":27313338,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":536,"end":698,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":139,"end":301,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":135,"end":297,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":237,"end":399,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":182,"end":344,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":182,"end":344,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":503,"end":665,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":271,"end":433,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":134,"end":296,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":183,"end":345,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":184,"end":346,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3964,"product_size":163,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":7,"left":"AGAGCCGGTGTGAGAAGAAG","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":165,"primer3_penalty":0.6414,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":19,"paired_amplicon_count":5,"target_amplicon_count":0,"off_target_amplicon_count":5,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398756,"end":23398920,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322341,"product_size":163,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538380,"end":73538544,"product_size":165,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000071.7","start":31514083,"end":31515010,"product_size":928,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000074.7","start":27313174,"end":27313338,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":534,"end":698,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":137,"end":301,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":133,"end":297,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":235,"end":399,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":180,"end":344,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":180,"end":344,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":501,"end":665,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":269,"end":433,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":132,"end":296,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":181,"end":345,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":182,"end":346,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3966,"product_size":165,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"GCCGGACCTTTGAGTACACC","right":"GGTCACGGGCCAAATCACTA","transcript_product_size_bp":105,"primer3_penalty":0.7085,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":19,"paired_amplicon_count":4,"target_amplicon_count":0,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":23398816,"end":23398920,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000086.8","start":73538380,"end":73538484,"product_size":105,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000074.7","start":27313234,"end":27313338,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000069.7","start":116322179,"end":116322281,"product_size":103,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":594,"end":698,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":197,"end":301,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":193,"end":297,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":295,"end":399,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":240,"end":344,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":240,"end":344,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":561,"end":665,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":329,"end":433,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":192,"end":296,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":241,"end":345,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":242,"end":346,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3802,"end":3906,"product_size":105,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"CAGAGCCGGTGTGAGAAGAA","right":"TCCGTTTCAGCGCCATAGTC","transcript_product_size_bp":133,"primer3_penalty":0.778,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":135145419,"end":135145551,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000068.8","start":23398755,"end":23398887,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322212,"end":116322342,"product_size":131,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538413,"end":73538545,"product_size":133,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000074.7","start":27313173,"end":27313305,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":18,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":533,"end":665,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":136,"end":268,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":132,"end":264,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":234,"end":366,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":179,"end":311,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":179,"end":311,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":500,"end":632,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":268,"end":400,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":131,"end":263,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":180,"end":312,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":181,"end":313,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3835,"end":3967,"product_size":133,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":10,"left":"CAGAGCCGGTGTGAGAAGAA","right":"CCGTTTCAGCGCCATAGTCT","transcript_product_size_bp":132,"primer3_penalty":0.7787,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_locus_accession":"NC_000069.7","target_locus_start":135144887,"target_locus_end":135173975,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":135145419,"end":135145550,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000068.8","start":23398755,"end":23398886,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000069.7","start":116322213,"end":116322342,"product_size":130,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":73538414,"end":73538545,"product_size":132,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000074.7","start":27313173,"end":27313304,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001356597.1","target_gene_id":"Ube2d3","target_gene_name":"Ube2d3","left_hit_count":17,"right_hit_count":17,"paired_amplicon_count":17,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":6,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001356597.1","start":533,"end":664,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356594.1","start":136,"end":267,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356595.1","start":132,"end":263,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356596.1","start":234,"end":365,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_001356598.1","start":179,"end":310,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"NM_025356.5","start":179,"end":310,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163216.1","start":500,"end":631,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163217.1","start":268,"end":399,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163218.1","start":131,"end":262,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163219.1","start":180,"end":311,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_036163220.1","start":181,"end":312,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ube2d3","gene_name":"Ube2d3"},{"transcript_accession":"XM_006498412.5","start":3836,"end":3967,"product_size":132,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Spopl","gene_name":"Spopl"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"12841603a1","gene":"Cinp","target_transcript":"NM_027223.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TACAGGGAAGAAAGCAGCCG","right":"CGCCAATGGTGTGCTTCAAA","transcript_product_size_bp":124,"primer3_penalty":0.0682,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110840498,"end":110843332,"product_size":2835,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":6,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":602,"end":725,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":452,"end":575,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTTCACCACAGCGAGCAGTA","right":"CACAGTGCTTCGAGTCCCTT","transcript_product_size_bp":144,"primer3_penalty":0.0723,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110846150,"end":110850368,"product_size":4219,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":205,"end":348,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":205,"end":348,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGGATCGCAACACCAAGAA","right":"TACTGCTCGCTGTGGTGAAG","transcript_product_size_bp":134,"primer3_penalty":0.1478,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110850349,"end":110850482,"product_size":134,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":2,"right_hit_count":13,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":91,"end":224,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":91,"end":224,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TACAGGGAAGAAAGCAGCCG","right":"AGGTAGGTCAGGCTGAGGTT","transcript_product_size_bp":170,"primer3_penalty":0.1537,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110840452,"end":110843332,"product_size":2881,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":602,"end":771,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":452,"end":621,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TACAGGGAAGAAAGCAGCCG","right":"GGTAGGTCAGGCTGAGGTTG","transcript_product_size_bp":169,"primer3_penalty":0.2869,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110840453,"end":110843332,"product_size":2880,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":6,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":602,"end":770,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":452,"end":620,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGGACAGCCTCAGTGACAAG","right":"CACAGTGCTTCGAGTCCCTT","transcript_product_size_bp":166,"primer3_penalty":0.3552,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110846150,"end":110850390,"product_size":4241,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":183,"end":348,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":183,"end":348,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CTCTTGGGATCGCAACACCA","right":"TACTGCTCGCTGTGGTGAAG","transcript_product_size_bp":138,"primer3_penalty":0.3598,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110850349,"end":110850486,"product_size":138,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":2,"right_hit_count":13,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":87,"end":224,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":87,"end":224,"product_size":138,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ACTCTTGGGATCGCAACACC","right":"TACTGCTCGCTGTGGTGAAG","transcript_product_size_bp":139,"primer3_penalty":0.3599,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110850349,"end":110850487,"product_size":139,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":2,"right_hit_count":13,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":86,"end":224,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":86,"end":224,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AGGACAATGCTGCTGACTGG","right":"TACTGCTCGCTGTGGTGAAG","transcript_product_size_bp":81,"primer3_penalty":0.3605,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110850349,"end":110850429,"product_size":81,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":2,"right_hit_count":13,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":144,"end":224,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":144,"end":224,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TACAGGGAAGAAAGCAGCCG","right":"GTAGGTCAGGCTGAGGTTGC","transcript_product_size_bp":168,"primer3_penalty":0.4282,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_locus_accession":"NC_000078.7","target_locus_start":110839044,"target_locus_end":110855579,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":110840454,"end":110843332,"product_size":2879,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027223.1","target_gene_id":"Cinp","target_gene_name":"Cinp","left_hit_count":6,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027223.1","start":602,"end":769,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cinp","gene_name":"Cinp"},{"transcript_accession":"NM_026048.4","start":452,"end":619,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Cinp","gene_name":"Cinp"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"19263800a1","gene":"Arhgef5","target_transcript":"NM_133674.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TTGTGGGCCATGTGAGACTC","right":"CCTGGGAACGAGTTGCTTCT","transcript_product_size_bp":141,"primer3_penalty":0.0722,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250897,"end":43251037,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":1759,"end":1899,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":1704,"end":1844,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTTCACCACCCACTGTCTCC","right":"GAGGAGGATGACTATGCCGC","transcript_product_size_bp":153,"primer3_penalty":0.0732,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43251334,"end":43251486,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":2196,"end":2348,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":2141,"end":2293,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGAAGAGGGGTTTAACGGCA","right":"GGTCCTGTCCCTCCTCTTCT","transcript_product_size_bp":194,"primer3_penalty":0.0791,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250033,"end":43250226,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":895,"end":1088,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":840,"end":1033,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGGAAGAGGGGTTTAACGGC","right":"GGTCCTGTCCCTCCTCTTCT","transcript_product_size_bp":195,"primer3_penalty":0.0791,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250032,"end":43250226,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":894,"end":1088,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":839,"end":1033,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TTAGGCAGGGGAAACAGCTG","right":"AAACCCCTCTTCCACCTCCT","transcript_product_size_bp":197,"primer3_penalty":0.142,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43249849,"end":43250045,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":711,"end":907,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":656,"end":852,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CCAGTGGCTTCCGTAGATCC","right":"TCAGGAGCAAAAGAGGCAGG","transcript_product_size_bp":191,"primer3_penalty":0.1423,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250472,"end":43250662,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":1334,"end":1524,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":1279,"end":1469,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGAAACCTCCCCAGACACCT","right":"CAGCTGTTTCCCCTGCCTAA","transcript_product_size_bp":121,"primer3_penalty":0.1426,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43249748,"end":43249868,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":610,"end":730,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":555,"end":675,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGACAGCAGTGGAAGGGAG","right":"TCAGTTCCTCACACACAGGC","transcript_product_size_bp":166,"primer3_penalty":0.1433,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250421,"end":43250586,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":1283,"end":1448,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":1228,"end":1393,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGACAGCAGTGGAAGGGAG","right":"GGCTCAGTTCCTCACACACA","transcript_product_size_bp":169,"primer3_penalty":0.1433,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250421,"end":43250589,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":1283,"end":1451,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":1228,"end":1396,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGTTTTCTCCAGCACTGCCA","right":"GAGTCTCACATGGCCCACAA","transcript_product_size_bp":119,"primer3_penalty":0.1435,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_locus_accession":"NC_000072.7","target_locus_start":43242578,"target_locus_end":43266250,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":43250798,"end":43250916,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133674.2","target_gene_id":"Arhgef5","target_gene_name":"Arhgef5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133674.2","start":1660,"end":1778,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Arhgef5","gene_name":"Arhgef5"},{"transcript_accession":"NM_001417622.1","start":1605,"end":1723,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Arhgef5","gene_name":"Arhgef5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"33239401a1","gene":"Mmgt2","target_transcript":"NM_175002.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"AGTTCCCCGATGCTTTCCTG","right":"CCAGAGCTAGCCTTCCACAC","transcript_product_size_bp":170,"primer3_penalty":0.143,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":161,"end":330,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":193,"end":362,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":193,"end":464,"product_size":272,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":193,"end":568,"product_size":376,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":193,"end":647,"product_size":455,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":193,"end":670,"product_size":478,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TTCCCCGATGCTTTCCTGAC","right":"CCAGAGCTAGCCTTCCACAC","transcript_product_size_bp":168,"primer3_penalty":0.1434,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":163,"end":330,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":195,"end":362,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":195,"end":464,"product_size":270,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":195,"end":568,"product_size":374,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":195,"end":647,"product_size":453,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":195,"end":670,"product_size":476,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GTTCCCCGATGCTTTCCTGA","right":"CCAGAGCTAGCCTTCCACAC","transcript_product_size_bp":169,"primer3_penalty":0.1434,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":7,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":162,"end":330,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":194,"end":362,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":194,"end":464,"product_size":271,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":194,"end":568,"product_size":375,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":194,"end":647,"product_size":454,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":194,"end":670,"product_size":477,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGAGTTCCCCGATGCTTTCC","right":"CCAGAGCTAGCCTTCCACAC","transcript_product_size_bp":172,"primer3_penalty":0.1434,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":159,"end":330,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":191,"end":362,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":191,"end":464,"product_size":274,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":191,"end":568,"product_size":378,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":191,"end":647,"product_size":457,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":191,"end":670,"product_size":480,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CAGTGAGTTCCCCGATGCTT","right":"CCAGAGCTAGCCTTCCACAC","transcript_product_size_bp":175,"primer3_penalty":0.144,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":7,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":156,"end":330,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":188,"end":362,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":188,"end":464,"product_size":277,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":188,"end":568,"product_size":381,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":188,"end":647,"product_size":460,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":188,"end":670,"product_size":483,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCCGGCGGATATAGTTCTCC","right":"AACAGTCGGTAGCCAGAACG","transcript_product_size_bp":180,"primer3_penalty":0.1473,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":62555776,"end":62555955,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":474,"end":653,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":506,"end":685,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_011248909.3","start":925,"end":1104,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156555.1","start":466,"end":645,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":814,"end":993,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":712,"end":891,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":608,"end":787,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156559.1","start":672,"end":851,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":791,"end":970,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGTTCCCCGATGCTTTCCTG","right":"CCTGTAGCAGCGCAGTACAT","transcript_product_size_bp":81,"primer3_penalty":0.2142,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":161,"end":241,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":193,"end":273,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":193,"end":375,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":193,"end":479,"product_size":287,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":193,"end":558,"product_size":366,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":193,"end":581,"product_size":389,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GTTCCCCGATGCTTTCCTGA","right":"CCTGTAGCAGCGCAGTACAT","transcript_product_size_bp":80,"primer3_penalty":0.2145,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":162,"end":241,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":194,"end":273,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":194,"end":375,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":194,"end":479,"product_size":286,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":194,"end":558,"product_size":365,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":194,"end":581,"product_size":388,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TGAGTTCCCCGATGCTTTCC","right":"CCTGTAGCAGCGCAGTACAT","transcript_product_size_bp":83,"primer3_penalty":0.2145,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":159,"end":241,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":191,"end":273,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":191,"end":375,"product_size":185,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":191,"end":479,"product_size":289,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":191,"end":558,"product_size":368,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":191,"end":581,"product_size":391,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGTGAGTTCCCCGATGCTT","right":"CCTGTAGCAGCGCAGTACAT","transcript_product_size_bp":86,"primer3_penalty":0.2151,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_locus_accession":"NC_000077.7","target_locus_start":62539490,"target_locus_end":62557185,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_175002.2","target_gene_id":"Mmgt2","target_gene_name":"Mmgt2","left_hit_count":6,"right_hit_count":9,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_175002.2","start":156,"end":241,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_006532870.2","start":188,"end":273,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156558.1","start":188,"end":375,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156557.1","start":188,"end":479,"product_size":292,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156560.1","start":188,"end":558,"product_size":371,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"},{"transcript_accession":"XM_036156556.1","start":188,"end":581,"product_size":394,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mmgt2","gene_name":"Mmgt2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"30794198a1","gene":"Nkrf","target_transcript":"NM_029891.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GAAATGAAATCCAGGGCCGC","right":"CCAGCCCATCTTTCTCAGCA","transcript_product_size_bp":95,"primer3_penalty":0.1378,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152589,"end":36152683,"product_size":95,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2344,"end":2438,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACAGGTCTCCGCTGGAAATG","right":"GCGGCCCTGGATTTCATTTC","transcript_product_size_bp":198,"primer3_penalty":0.139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152664,"end":36152861,"product_size":198,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2166,"end":2363,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCTGAGAAAGATGGGCTGG","right":"CTAGACCAAGGCCTTCTCGC","transcript_product_size_bp":108,"primer3_penalty":0.2139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152501,"end":36152608,"product_size":108,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2419,"end":2526,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAAATGAAATCCAGGGCCGC","right":"TTTCCCTAAACCACCACCCG","transcript_product_size_bp":116,"primer3_penalty":0.2139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152568,"end":36152683,"product_size":116,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2344,"end":2459,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCACGGAGCTGACCAATGAT","right":"GCCCACCCCATGAGACTTAC","transcript_product_size_bp":86,"primer3_penalty":0.2147,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152322,"end":36152407,"product_size":86,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2620,"end":2705,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTAAGTCTCATGGGGTGGGC","right":"GGCCCTCCTGTTTGAGTTGA","transcript_product_size_bp":93,"primer3_penalty":0.2177,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152249,"end":36152341,"product_size":93,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2778,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCACGGAGCTGACCAATGAT","right":"GGCCCTCCTGTTTGAGTTGA","transcript_product_size_bp":159,"primer3_penalty":0.218,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152249,"end":36152407,"product_size":159,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2620,"end":2778,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CGGGTGGTGGTTTAGGGAAA","right":"ATCATTGGTCAGCTCCGTGG","transcript_product_size_bp":200,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152388,"end":36152587,"product_size":200,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2440,"end":2639,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GTAAGTCTCATGGGGTGGGC","right":"CTTGGCCCTCCTGTTTGAGT","transcript_product_size_bp":96,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152246,"end":36152341,"product_size":96,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2781,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GTAAGTCTCATGGGGTGGGC","right":"ACTTGGCCCTCCTGTTTGAG","transcript_product_size_bp":97,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152245,"end":36152341,"product_size":97,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2782,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26347907a1","gene":"Hmgcr","target_transcript":"NM_001360166.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGTGGCCAGCACTAACAGAG","right":"CCTCGAGTCATCCCATCTGC","transcript_product_size_bp":87,"primer3_penalty":0.0685,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96792166,"end":96792398,"product_size":233,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":2242,"end":2328,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":2195,"end":2281,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":1832,"end":1918,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":2324,"end":2410,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGTGGCCAGCACTAACAGAG","right":"GCCTCGAGTCATCCCATCTG","transcript_product_size_bp":88,"primer3_penalty":0.0685,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96792165,"end":96792398,"product_size":234,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":2242,"end":2329,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":2195,"end":2282,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":1832,"end":1919,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":2324,"end":2411,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CTGTCTTGTGGCCAGCACTA","right":"CCTCGAGTCATCCCATCTGC","transcript_product_size_bp":93,"primer3_penalty":0.0685,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96792166,"end":96792404,"product_size":239,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":2236,"end":2328,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":2189,"end":2281,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":1826,"end":1918,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":2318,"end":2410,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGTCTTGTGGCCAGCACTA","right":"GCCTCGAGTCATCCCATCTG","transcript_product_size_bp":94,"primer3_penalty":0.0685,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96792165,"end":96792404,"product_size":240,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":2236,"end":2329,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":2189,"end":2282,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":1826,"end":1919,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":2318,"end":2411,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCCGTCGTGACCTCAAAGAA","right":"CTCTCTTGGTTCGCTCCTGG","transcript_product_size_bp":116,"primer3_penalty":0.1414,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":8,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96795384,"end":96795499,"product_size":116,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":1628,"end":1743,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":1581,"end":1696,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":1218,"end":1333,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":1710,"end":1825,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGCCCCACATTCACTCTTGA","right":"AGGCCGGGAAGAATGTCATG","transcript_product_size_bp":151,"primer3_penalty":0.1452,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96796646,"end":96799600,"product_size":2955,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":1052,"end":1202,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":1005,"end":1155,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":642,"end":792,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":1134,"end":1284,"product_size":151,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGCCCCACATTCACTCTTGA","right":"CAGGCCGGGAAGAATGTCAT","transcript_product_size_bp":152,"primer3_penalty":0.1452,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96796645,"end":96799600,"product_size":2956,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":1052,"end":1203,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":1005,"end":1156,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":642,"end":793,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":1134,"end":1285,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGCCCCACATTCACTCTTGA","right":"GGCCGGGAAGAATGTCATGA","transcript_product_size_bp":150,"primer3_penalty":0.1454,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96796647,"end":96799600,"product_size":2954,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":1052,"end":1201,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":1005,"end":1154,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":642,"end":791,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":1134,"end":1283,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGCCCCACATTCACTCTTGA","right":"CTAGGACCAGCGACACACAG","transcript_product_size_bp":169,"primer3_penalty":0.1473,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96796628,"end":96799600,"product_size":2973,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":1052,"end":1220,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":1005,"end":1173,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_008255.2","start":642,"end":810,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"XM_036157854.1","start":1134,"end":1302,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCTGCAAATTGACCCGATCC","right":"GCCATGCATCCGGAAAAGTC","transcript_product_size_bp":126,"primer3_penalty":0.2024,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_locus_accession":"NC_000079.7","target_locus_start":96785470,"target_locus_end":96807444,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":96803164,"end":96806981,"product_size":3818,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001360166.1","target_gene_id":"Hmgcr","target_gene_name":"Hmgcr","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001360166.1","start":464,"end":589,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hmgcr","gene_name":"Hmgcr"},{"transcript_accession":"NM_001360165.1","start":464,"end":542,"product_size":79,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Hmgcr","gene_name":"Hmgcr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"11464985a1","gene":"Olfr71","target_transcript":"NM_019486.1","candidate_pairs_screened":10,"combined_pass_pairs":9,"transcript_gene_specific_pairs":9,"transcript_isoform_specific_pairs":9,"candidates":[{"rank":1,"left":"AAAGCCTTCTCCACCTGCTC","right":"CGCTTCCTTCACCTCCTTGT","transcript_product_size_bp":195,"primer3_penalty":0.0729,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":46,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43705667,"end":43705861,"product_size":195,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":44,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":706,"end":900,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AAAGCCTTCTCCACCTGCTC","right":"CCTCCTTGTTCCTCAGGCTG","transcript_product_size_bp":184,"primer3_penalty":0.0731,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":46,"right_hit_count":40,"paired_amplicon_count":4,"target_amplicon_count":1,"off_target_amplicon_count":3,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43705678,"end":43705861,"product_size":184,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000070.7","start":43770100,"end":43770301,"product_size":202,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000070.7","start":43781416,"end":43781617,"product_size":202,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000070.7","start":43835582,"end":43835783,"product_size":202,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":43,"right_hit_count":46,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":706,"end":889,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"},{"transcript_accession":"NM_019475.3","start":735,"end":936,"product_size":202,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Or13c7c","gene_name":"Or13c7c"},{"transcript_accession":"NM_019476.1","start":709,"end":910,"product_size":202,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Or13c7d","gene_name":"Or13c7d"},{"transcript_accession":"NR_033638.1","start":711,"end":912,"product_size":202,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Or13c7e-ps1","gene_name":"Or13c7e-ps1"},{"transcript_accession":"XM_006537504.1","start":914,"end":1115,"product_size":202,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Or13c7c","gene_name":"Or13c7c"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":3,"left":"TGACCGCTACTTGGCCATTT","right":"ACTTGAAGAGGCAGAGCACC","transcript_product_size_bp":107,"primer3_penalty":0.0736,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":13,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706101,"end":43706207,"product_size":107,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":360,"end":466,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGTGCTCTGCCTCTTCAAGT","right":"GAGGGTCAGGGGTATAGGCA","transcript_product_size_bp":193,"primer3_penalty":0.1412,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43705928,"end":43706120,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":447,"end":639,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGTGCTCTGCCTCTTCAAGT","right":"TGAGGGTCAGGGGTATAGGC","transcript_product_size_bp":194,"primer3_penalty":0.1412,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43705927,"end":43706120,"product_size":194,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":447,"end":640,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ATCTCCTTTACGGGCTGTGC","right":"ACTTGAAGAGGCAGAGCACC","transcript_product_size_bp":193,"primer3_penalty":0.1437,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706101,"end":43706293,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":274,"end":466,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGTGCTCTGCCTCTTCAAGT","right":"CAGCCAGGATCTCACAGGTG","transcript_product_size_bp":104,"primer3_penalty":0.1438,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706017,"end":43706120,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":447,"end":550,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGTGCTCTGCCTCTTCAAGT","right":"CACAGCCAGGATCTCACAGG","transcript_product_size_bp":106,"primer3_penalty":0.1438,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706015,"end":43706120,"product_size":106,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":447,"end":552,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TGACCGCTACTTGGCCATTT","right":"CAGCCAGGATCTCACAGGTG","transcript_product_size_bp":191,"primer3_penalty":0.1454,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":7,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706017,"end":43706207,"product_size":191,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":5,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":360,"end":550,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGACCGCTACTTGGCCATTT","right":"CACAGCCAGGATCTCACAGG","transcript_product_size_bp":193,"primer3_penalty":0.1454,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_locus_accession":"NC_000070.7","target_locus_start":43705628,"target_locus_end":43706566,"target_locus_strand":"-","left_hit_count":6,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":43706015,"end":43706207,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019486.1","target_gene_id":"Or13j1","target_gene_name":"Or13j1","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019486.1","start":360,"end":552,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or13j1","gene_name":"Or13j1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31982604a1","gene":"Ogfr","target_transcript":"NM_031373.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CTGTCTTGTGGCTAGGGTGG","right":"CTGGCCCATTCCCATCCTTT","transcript_product_size_bp":161,"primer3_penalty":0.0678,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236661,"end":180236821,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1319,"end":1479,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1304,"end":1464,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1415,"end":1575,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCTGTCTTGTGGCTAGGGTG","right":"CTGGCCCATTCCCATCCTTT","transcript_product_size_bp":162,"primer3_penalty":0.0678,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236660,"end":180236821,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1318,"end":1479,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1303,"end":1464,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1414,"end":1575,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATGAGGCTAAGTCCCCGAGT","right":"CACCCTAGCCACAAGACAGG","transcript_product_size_bp":200,"primer3_penalty":0.068,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236480,"end":180236679,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1138,"end":1337,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1123,"end":1322,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1234,"end":1433,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GATAAAGATGAGGGCCCCGG","right":"ACTCGGGGACTTAGCCTCAT","transcript_product_size_bp":183,"primer3_penalty":0.0689,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236317,"end":180236499,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":7,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":975,"end":1157,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":960,"end":1142,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1071,"end":1253,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GATAAAGATGAGGGCCCCGG","right":"CTGTTCCACTGTCCCCACTC","transcript_product_size_bp":97,"primer3_penalty":0.0723,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236317,"end":180236413,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":975,"end":1071,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":960,"end":1056,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1071,"end":1167,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGAAGTTGGAGGGGAACAGG","right":"CACCCTAGCCACAAGACAGG","transcript_product_size_bp":161,"primer3_penalty":0.0743,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236519,"end":180236679,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1177,"end":1337,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1162,"end":1322,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1273,"end":1433,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGAAGTTGGAGGGGAACAGG","right":"CCACCCTAGCCACAAGACAG","transcript_product_size_bp":162,"primer3_penalty":0.0743,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236519,"end":180236680,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1177,"end":1338,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1162,"end":1323,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1273,"end":1434,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGGAAGGAGCTGAGGGTGAT","right":"CTGGCCCATTCCCATCCTTT","transcript_product_size_bp":111,"primer3_penalty":0.0758,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236711,"end":180236821,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1369,"end":1479,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1354,"end":1464,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1465,"end":1575,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCTGGGCTTCTATGGGATCC","right":"GCAGATTGTTGTGGCTGTGG","transcript_product_size_bp":113,"primer3_penalty":0.0763,"exon_span":{"spans_junction":false,"left_exon":5,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180235419,"end":180236020,"product_size":602,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":566,"end":678,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":551,"end":663,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":662,"end":774,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTGTCTTGTGGCTAGGGTGG","right":"TGAAGGGGTAGGAGACAGGG","transcript_product_size_bp":124,"primer3_penalty":0.0787,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_locus_accession":"NC_000068.8","target_locus_start":180231200,"target_locus_end":180237630,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":9,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":180236661,"end":180236784,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031373.3","target_gene_id":"Ogfr","target_gene_name":"Ogfr","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031373.3","start":1319,"end":1442,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_017319269.3","start":1304,"end":1427,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"},{"transcript_accession":"XM_036162586.1","start":1415,"end":1538,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ogfr","gene_name":"Ogfr"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13654260a1","gene":"Slc19a1","target_transcript":"NM_031196.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TATCACCTGCTTTGCCTGGG","right":"TCCTCCATGCCATCCTCTGA","transcript_product_size_bp":195,"primer3_penalty":0.065,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885409,"end":76885603,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1491,"end":1685,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1397,"end":1591,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1620,"end":1814,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1600,"end":1794,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1377,"end":1571,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1479,"end":1673,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1342,"end":1536,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1567,"end":1761,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":1968,"end":2162,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1321,"end":1515,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1438,"end":1632,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1457,"end":1651,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGACGCAGAGCTAGGAATCC","right":"ATGTTGCCCAGGTCCTCATG","transcript_product_size_bp":107,"primer3_penalty":0.0666,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":10,"right_hit_count":18,"paired_amplicon_count":10,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":9,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":79,"end":185,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":93,"end":199,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":91,"end":197,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":89,"end":195,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":89,"end":195,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936131.1","start":90,"end":196,"product_size":107,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":90,"end":294,"product_size":205,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936133.1","start":90,"end":294,"product_size":205,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":105,"end":314,"product_size":210,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948634.2","start":90,"end":299,"product_size":210,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGACGCAGAGCTAGGAATCC","right":"CATGTTGCCCAGGTCCTCAT","transcript_product_size_bp":108,"primer3_penalty":0.0666,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":10,"right_hit_count":18,"paired_amplicon_count":10,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":9,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":79,"end":186,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":93,"end":200,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":91,"end":198,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":89,"end":196,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":89,"end":196,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936131.1","start":90,"end":197,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":90,"end":295,"product_size":206,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936133.1","start":90,"end":295,"product_size":206,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":105,"end":315,"product_size":211,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948634.2","start":90,"end":300,"product_size":211,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGGACGCAGAGCTAGGAATC","right":"ATGTTGCCCAGGTCCTCATG","transcript_product_size_bp":108,"primer3_penalty":0.0666,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":10,"right_hit_count":18,"paired_amplicon_count":10,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":9,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":78,"end":185,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":92,"end":199,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":90,"end":197,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":88,"end":195,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":88,"end":195,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936131.1","start":89,"end":196,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":89,"end":294,"product_size":206,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936133.1","start":89,"end":294,"product_size":206,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":104,"end":314,"product_size":211,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948634.2","start":89,"end":299,"product_size":211,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGGACGCAGAGCTAGGAATC","right":"CATGTTGCCCAGGTCCTCAT","transcript_product_size_bp":109,"primer3_penalty":0.0666,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":10,"right_hit_count":18,"paired_amplicon_count":10,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":9,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":78,"end":186,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":92,"end":200,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":90,"end":198,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":88,"end":196,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":88,"end":196,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936131.1","start":89,"end":197,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":89,"end":295,"product_size":207,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_004936133.1","start":89,"end":295,"product_size":207,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":104,"end":315,"product_size":212,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948634.2","start":89,"end":300,"product_size":212,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCAGAGGATGGCATGGAGGA","right":"AACATCGACTGGGAGCACTG","transcript_product_size_bp":178,"primer3_penalty":0.0678,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885584,"end":76885761,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1666,"end":1843,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1572,"end":1749,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1795,"end":1972,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1775,"end":1952,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1552,"end":1729,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1654,"end":1831,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1517,"end":1694,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1742,"end":1919,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":2143,"end":2320,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1496,"end":1673,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1613,"end":1790,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1632,"end":1809,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCAGAGGATGGCATGGAGGA","right":"CAACATCGACTGGGAGCACT","transcript_product_size_bp":179,"primer3_penalty":0.0678,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885584,"end":76885762,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1666,"end":1844,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1572,"end":1750,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1795,"end":1973,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1775,"end":1953,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1552,"end":1730,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1654,"end":1832,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1517,"end":1695,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1742,"end":1920,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":2143,"end":2321,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1496,"end":1674,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1613,"end":1791,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1632,"end":1810,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTCCCAGTCGATGTTGCCTT","right":"GGAGGGTTCTGTGGCCTAAC","transcript_product_size_bp":91,"primer3_penalty":0.0714,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885747,"end":76885837,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1829,"end":1919,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1735,"end":1825,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1958,"end":2048,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1938,"end":2028,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1715,"end":1805,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1817,"end":1907,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1680,"end":1770,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1905,"end":1995,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":2306,"end":2396,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1659,"end":1749,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1776,"end":1866,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1795,"end":1885,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AGTGCTCCCAGTCGATGTTG","right":"GGAGGGTTCTGTGGCCTAAC","transcript_product_size_bp":95,"primer3_penalty":0.0723,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885743,"end":76885837,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1825,"end":1919,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1731,"end":1825,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1954,"end":2048,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1934,"end":2028,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1711,"end":1805,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1813,"end":1907,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1676,"end":1770,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1901,"end":1995,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":2302,"end":2396,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1655,"end":1749,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1772,"end":1866,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1791,"end":1885,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGTGCTCCCAGTCGATGTT","right":"GGAGGGTTCTGTGGCCTAAC","transcript_product_size_bp":96,"primer3_penalty":0.0723,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_locus_accession":"NC_000076.7","target_locus_start":76869046,"target_locus_end":76886266,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000076.7","start":76885742,"end":76885837,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031196.4","target_gene_id":"Slc19a1","target_gene_name":"Slc19a1","left_hit_count":18,"right_hit_count":18,"paired_amplicon_count":18,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":17,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031196.3","start":1824,"end":1919,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"NM_001199271.1","start":1730,"end":1825,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513414.5","start":1953,"end":2048,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513415.5","start":1933,"end":2028,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513416.4","start":1710,"end":1805,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513417.4","start":1812,"end":1907,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_006513419.5","start":1675,"end":1770,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_030244971.2","start":1900,"end":1995,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155713.1","start":2301,"end":2396,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XM_036155714.1","start":1654,"end":1749,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948631.2","start":1771,"end":1866,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"},{"transcript_accession":"XR_003948632.2","start":1790,"end":1885,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc19a1","gene_name":"Slc19a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26337679a1","gene":"Lrguk","target_transcript":"NM_001401521.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CACCTTAGTCTGGCTGGCAA","right":"AGCTCCTCCAGACCTGTGAT","transcript_product_size_bp":116,"primer3_penalty":0.0792,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":749,"end":864,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":566,"end":681,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_028886.2","start":749,"end":864,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":930,"end":1045,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACCTTAGTCTGGCTGGCAAC","right":"AGCTCCTCCAGACCTGTGAT","transcript_product_size_bp":115,"primer3_penalty":0.0793,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":750,"end":864,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":567,"end":681,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_028886.2","start":750,"end":864,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":931,"end":1045,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGATGGAAAGGCACCCGTTT","right":"TGGAGGAGGTAAGAGGAGGC","transcript_product_size_bp":164,"primer3_penalty":0.1444,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34006419,"end":34006582,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":56,"end":219,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":56,"end":219,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_028886.2","start":56,"end":219,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":237,"end":400,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCCAGAAATTCCACCTCCCC","right":"TTGTCCCCAAAGCCTCTTCC","transcript_product_size_bp":182,"primer3_penalty":0.1455,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34079421,"end":34079602,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":2683,"end":2864,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":2503,"end":2684,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":2867,"end":3048,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_011241496.3","start":997,"end":1178,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CAGACCAAGCCCGAGTACTG","right":"TGTGATCTTGGACAGCGACC","transcript_product_size_bp":148,"primer3_penalty":0.1459,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34049302,"end":34050708,"product_size":1407,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":1046,"end":1193,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":863,"end":1010,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_028886.2","start":1046,"end":1193,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":1227,"end":1374,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CCTCCCCAAGACTCAACCAC","right":"TTGTCCCCAAAGCCTCTTCC","transcript_product_size_bp":169,"primer3_penalty":0.1493,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34079434,"end":34079602,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":2696,"end":2864,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":2516,"end":2684,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":2880,"end":3048,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_011241496.3","start":1010,"end":1178,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CACCTCCCCAAGACTCAACC","right":"TTGTCCCCAAAGCCTCTTCC","transcript_product_size_bp":171,"primer3_penalty":0.1493,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34079432,"end":34079602,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":2694,"end":2864,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":2514,"end":2684,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":2878,"end":3048,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_011241496.3","start":1008,"end":1178,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCACCTCCCCAAGACTCAAC","right":"TTGTCCCCAAAGCCTCTTCC","transcript_product_size_bp":172,"primer3_penalty":0.1493,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34079431,"end":34079602,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":5,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":2693,"end":2864,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":2513,"end":2684,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":2877,"end":3048,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_011241496.3","start":1007,"end":1178,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCCAGAAATTCCACCTCCCC","right":"TGGGAGAGAGAAGCTTGGGT","transcript_product_size_bp":158,"primer3_penalty":0.212,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":34079421,"end":34079578,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":2683,"end":2840,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":2503,"end":2660,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":2867,"end":3024,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_011241496.3","start":997,"end":1154,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CACCTTAGTCTGGCTGGCAA","right":"GCTCCTCCAGACCTGTGATG","transcript_product_size_bp":115,"primer3_penalty":0.2143,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_locus_accession":"NC_000072.7","target_locus_start":34006364,"target_locus_end":34088145,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001401521.1","target_gene_id":"Lrguk","target_gene_name":"Lrguk","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001401521.1","start":749,"end":863,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_001401523.1","start":566,"end":680,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"NM_028886.2","start":749,"end":863,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"},{"transcript_accession":"XM_006506713.5","start":930,"end":1044,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Lrguk","gene_name":"Lrguk"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13385202a1","gene":"Trpd52l3","target_transcript":"NM_025741.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GTGTGGCGAACTCAAGAGGA","right":"AGGGCAGTTGACGTCTTCTG","transcript_product_size_bp":126,"primer3_penalty":0.0676,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981576,"end":29981701,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":387,"end":512,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GTGTGGCGAACTCAAGAGGA","right":"CAGGGCAGTTGACGTCTTCT","transcript_product_size_bp":127,"primer3_penalty":0.0676,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981576,"end":29981702,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":387,"end":513,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGGCTGAGCAACTGGAACTC","right":"TCCTCTTGAGTTCGCCACAC","transcript_product_size_bp":111,"primer3_penalty":0.0695,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981485,"end":29981595,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":296,"end":406,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAGGCTGAGCAACTGGAACT","right":"TCCTCTTGAGTTCGCCACAC","transcript_product_size_bp":112,"primer3_penalty":0.0695,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981484,"end":29981595,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":295,"end":406,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CGTCCACAAATGAATCCCGC","right":"TCCTCTTGAGTTCGCCACAC","transcript_product_size_bp":156,"primer3_penalty":0.1322,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981440,"end":29981595,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":251,"end":406,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCGTCCACAAATGAATCCCG","right":"TCCTCTTGAGTTCGCCACAC","transcript_product_size_bp":157,"primer3_penalty":0.1322,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981439,"end":29981595,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":250,"end":406,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACTTACGTGACCTGCTTGCA","right":"AGGGCAGTTGACGTCTTCTG","transcript_product_size_bp":160,"primer3_penalty":0.14,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981542,"end":29981701,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":353,"end":512,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ACTTACGTGACCTGCTTGCA","right":"CAGGGCAGTTGACGTCTTCT","transcript_product_size_bp":161,"primer3_penalty":0.14,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981542,"end":29981702,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":353,"end":513,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TCCTATCCCACTGGCCAGAA","right":"CGGGATTCATTTGTGGACGC","transcript_product_size_bp":191,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981268,"end":29981458,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":79,"end":269,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCCTATCCCACTGGCCAGAA","right":"GCGGGATTCATTTGTGGACG","transcript_product_size_bp":192,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_locus_accession":"NC_000085.7","target_locus_start":29981190,"target_locus_end":29983420,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":29981268,"end":29981459,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025741.2","target_gene_id":"Trpd52l3","target_gene_name":"Trpd52l3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025741.2","start":79,"end":270,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Trpd52l3","gene_name":"Trpd52l3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"22324938a1","gene":"Prkcdbp","target_transcript":"NM_028444.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CAGTCGTAAAGCAGCACAGC","right":"GGTTGAGGAGGTTCTGGCTC","transcript_product_size_bp":150,"primer3_penalty":0.2006,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130080,"end":105130229,"product_size":150,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":620,"end":769,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCTGAAGAGGAGCCAGTGGA","right":"GCTGTGCTGCTTTACGACTG","transcript_product_size_bp":106,"primer3_penalty":0.2798,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130210,"end":105130315,"product_size":106,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":534,"end":639,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGAAGAGGAGCCAGTGGAGT","right":"GCTGTGCTGCTTTACGACTG","transcript_product_size_bp":104,"primer3_penalty":0.3436,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130210,"end":105130313,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":536,"end":639,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCTGAAGAGGAGCCAGTGGA","right":"CGGACTCCATTTCAGGCTGA","transcript_product_size_bp":199,"primer3_penalty":0.3654,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130117,"end":105130315,"product_size":199,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":534,"end":732,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TCTGAAGAGGAGCCAGTGGA","right":"TCGGACTCCATTTCAGGCTG","transcript_product_size_bp":200,"primer3_penalty":0.3654,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130116,"end":105130315,"product_size":200,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":534,"end":733,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAGTCGTAAAGCAGCACAGC","right":"CGGACTCCATTTCAGGCTGA","transcript_product_size_bp":113,"primer3_penalty":0.4149,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130117,"end":105130229,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":620,"end":732,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAGTCGTAAAGCAGCACAGC","right":"TCGGACTCCATTTCAGGCTG","transcript_product_size_bp":114,"primer3_penalty":0.4149,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130116,"end":105130229,"product_size":114,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":620,"end":733,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CAGTCGTAAAGCAGCACAGC","right":"GCACGGGTTTCTCAGGATCT","transcript_product_size_bp":179,"primer3_penalty":0.4152,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130051,"end":105130229,"product_size":179,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":620,"end":798,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGTCGTAAAGCAGCACAGC","right":"AGCACGGGTTTCTCAGGATC","transcript_product_size_bp":180,"primer3_penalty":0.4152,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130050,"end":105130229,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":620,"end":799,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCCAGTCGTAAAGCAGCACA","right":"GGTTGAGGAGGTTCTGGCTC","transcript_product_size_bp":152,"primer3_penalty":0.4271,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_locus_accession":"NC_000073.7","target_locus_start":105129823,"target_locus_end":105131404,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":105130080,"end":105130231,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028444.1","target_gene_id":"Cavin3","target_gene_name":"Cavin3","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028444.1","start":618,"end":769,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Cavin3","gene_name":"Cavin3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21717697a1","gene":"Vmn1r226","target_transcript":"NM_134191.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"ATGTCCATCAGCACCACCTG","right":"GCAGAGAGAAAAGAACAGGCC","transcript_product_size_bp":126,"primer3_penalty":1.8443,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20908049,"end":20908174,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":280,"end":405,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCAGTGATGTTGGGTGCAAAC","right":"GGAGTTCCTGGGGCTAATGG","transcript_product_size_bp":119,"primer3_penalty":1.8645,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907996,"end":20908114,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":227,"end":345,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CAGTGATGTTGGGTGCAAACT","right":"GGAGTTCCTGGGGCTAATGG","transcript_product_size_bp":118,"primer3_penalty":1.8666,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907997,"end":20908114,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":228,"end":345,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ATGTCCATCAGCACCACCTG","right":"AGAGAGAAAAGAACAGGCCCA","transcript_product_size_bp":124,"primer3_penalty":2.0927,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20908049,"end":20908172,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":3,"right_hit_count":12,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":280,"end":403,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ATGTCCATCAGCACCACCTG","right":"CAGAGAGAAAAGAACAGGCCCA","transcript_product_size_bp":125,"primer3_penalty":2.2594,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20908049,"end":20908173,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":280,"end":404,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TTCAGTGATGTTGGGTGCAAAC","right":"GGAGTTCCTGGGGCTAATGG","transcript_product_size_bp":120,"primer3_penalty":2.2843,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907995,"end":20908114,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":226,"end":345,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAGTGATGTTGGGTGCAAACTT","right":"GGAGTTCCTGGGGCTAATGG","transcript_product_size_bp":118,"primer3_penalty":2.285,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907997,"end":20908114,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":228,"end":345,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TCAGTGATGTTGGGTGCAAAC","right":"GTTCCTGGGGCTAATGGTGA","transcript_product_size_bp":116,"primer3_penalty":2.3045,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907996,"end":20908111,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":227,"end":342,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGTGATGTTGGGTGCAAACT","right":"GTTCCTGGGGCTAATGGTGA","transcript_product_size_bp":115,"primer3_penalty":2.3066,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907997,"end":20908111,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":228,"end":342,"product_size":115,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCAGTGATGTTGGGTGCAAAC","right":"AGTTCCTGGGGCTAATGGTG","transcript_product_size_bp":117,"primer3_penalty":2.3066,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_locus_accession":"NC_000083.7","target_locus_start":20907770,"target_locus_end":20908666,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":20907996,"end":20908112,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134191.1","target_gene_id":"Vmn1r226","target_gene_name":"Vmn1r226","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134191.1","start":227,"end":343,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r226","gene_name":"Vmn1r226"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"22095003a1","gene":"Sf3a3","target_transcript":"NM_029157.4","candidate_pairs_screened":10,"combined_pass_pairs":9,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TTCCGGCAGTGTTAGTGGTC","right":"CTCCTCATGATAGCGCCGTT","transcript_product_size_bp":90,"primer3_penalty":0.067,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124608640,"end":124608729,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":32,"end":121,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":32,"end":121,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":367,"end":456,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCCCAAACACTGCCCACTTT","right":"CCACCGTTCTGAAGCCTTCT","transcript_product_size_bp":95,"primer3_penalty":0.0681,"exon_span":{"spans_junction":true,"left_exon":14,"right_exon":15,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124623289,"end":124625204,"product_size":1916,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1398,"end":1492,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1491,"end":1585,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1733,"end":1827,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCTCCACGGCCTGAATATCA","right":"AAAGTGGGCAGTGTTTGGGA","transcript_product_size_bp":145,"primer3_penalty":0.1393,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124622135,"end":124623308,"product_size":1174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1273,"end":1417,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1366,"end":1510,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1608,"end":1752,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCCAAACACTGCCCACTTTG","right":"CCACCGTTCTGAAGCCTTCT","transcript_product_size_bp":94,"primer3_penalty":0.1401,"exon_span":{"spans_junction":true,"left_exon":14,"right_exon":15,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124623290,"end":124625204,"product_size":1915,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1399,"end":1492,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1492,"end":1585,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1734,"end":1827,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AACGGCGCTATCATGAGGAG","right":"CTATCTTGCATGGCCCGAGT","transcript_product_size_bp":124,"primer3_penalty":0.1401,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":102,"end":225,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":102,"end":225,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":437,"end":560,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CAAGCTCCACGGCCTGAATA","right":"AAAGTGGGCAGTGTTTGGGA","transcript_product_size_bp":148,"primer3_penalty":0.1403,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124622132,"end":124623308,"product_size":1177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1270,"end":1417,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1363,"end":1510,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1605,"end":1752,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TTCCGGCAGTGTTAGTGGTC","right":"CTATCTTGCATGGCCCGAGT","transcript_product_size_bp":194,"primer3_penalty":0.1418,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":32,"end":225,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":32,"end":225,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":367,"end":560,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGAGATGCTGCTGGAGTACC","right":"TGCTTGTCTCTTTCGGCCAT","transcript_product_size_bp":146,"primer3_penalty":0.1425,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"target_not_anchored","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":0,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":93142766,"end":93142910,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"Paired genomic products were found, but none fell within the annotated target locus."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":637,"end":782,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":730,"end":875,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":972,"end":1117,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":9,"left":"GCATGAGGTGTTTGGGCATC","right":"CCACCGTTCTGAAGCCTTCT","transcript_product_size_bp":113,"primer3_penalty":0.2096,"exon_span":{"spans_junction":true,"left_exon":14,"right_exon":15,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124623271,"end":124625204,"product_size":1934,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1380,"end":1492,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1473,"end":1585,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1715,"end":1827,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCTCCACGGCCTGAATATCA","right":"CAAAGTGGGCAGTGTTTGGG","transcript_product_size_bp":146,"primer3_penalty":0.2114,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_locus_accession":"NC_000070.7","target_locus_start":124608609,"target_locus_end":124626234,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":124622135,"end":124623309,"product_size":1175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029157.4","target_gene_id":"Sf3a3","target_gene_name":"Sf3a3","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029157.4","start":1273,"end":1418,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"NR_185053.1","start":1366,"end":1511,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"},{"transcript_accession":"XM_036164510.1","start":1608,"end":1753,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Sf3a3","gene_name":"Sf3a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"10181156a1","gene":"Taf1b","target_transcript":"NM_020614.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":1,"candidates":[{"rank":1,"left":"CCTGGAGACCCTCGGAGTAA","right":"CAGCTCAAGATCACTGCCCA","transcript_product_size_bp":181,"primer3_penalty":0.0691,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":5,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":4,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":321,"end":501,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGGCAGTGATCTTGAGCTG","right":"CGCTGACTTTGGGGATCCTT","transcript_product_size_bp":122,"primer3_penalty":0.0708,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":482,"end":603,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":384,"end":505,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGGCAGTGATCTTGAGCTG","right":"CGTCATCTTCACAAGCCCCT","transcript_product_size_bp":194,"primer3_penalty":0.0717,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24564815,"end":24566931,"product_size":2117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":482,"end":675,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":384,"end":577,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ATCCCCAAAGTCAGCGTCTG","right":"CGTCATCTTCACAAGCCCCT","transcript_product_size_bp":88,"primer3_penalty":0.072,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":588,"end":675,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":490,"end":577,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ATCCCCAAAGTCAGCGTCTG","right":"CAGAGAGCAAGTGTCCTGGG","transcript_product_size_bp":111,"primer3_penalty":0.0726,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":588,"end":698,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":490,"end":600,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCCACTCTACCGTTCACACA","right":"ACTTGAAGGGGCCTGCTTTT","transcript_product_size_bp":130,"primer3_penalty":0.1385,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24606010,"end":24606597,"product_size":588,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":1338,"end":1467,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":1240,"end":1369,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"XM_006515051.2","start":1094,"end":1223,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCCACTCTACCGTTCACACA","right":"AACTTGAAGGGGCCTGCTTT","transcript_product_size_bp":131,"primer3_penalty":0.1385,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24606010,"end":24606598,"product_size":589,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":1338,"end":1468,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":1240,"end":1370,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"XM_006515051.2","start":1094,"end":1224,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GCCACTCTACCGTTCACACA","right":"AAACTTGAAGGGGCCTGCTT","transcript_product_size_bp":132,"primer3_penalty":0.1385,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24606010,"end":24606599,"product_size":590,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":1338,"end":1469,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":1240,"end":1371,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"XM_006515051.2","start":1094,"end":1225,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCCACTCTACCGTTCACACA","right":"AAAACTTGAAGGGGCCTGCT","transcript_product_size_bp":133,"primer3_penalty":0.1385,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24606010,"end":24606600,"product_size":591,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":1338,"end":1470,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":1240,"end":1372,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"XM_006515051.2","start":1094,"end":1226,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GCCACTCTACCGTTCACACA","right":"TGAAGGGGCCTGCTTTTCAA","transcript_product_size_bp":127,"primer3_penalty":0.1387,"exon_span":{"spans_junction":true,"left_exon":12,"right_exon":13,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_locus_accession":"NC_000078.7","target_locus_start":24548580,"target_locus_end":24608570,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":24606010,"end":24606594,"product_size":585,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_020614.2","target_gene_id":"Taf1b","target_gene_name":"Taf1b","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_020614.2","start":1338,"end":1464,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"NR_028341.1","start":1240,"end":1366,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"},{"transcript_accession":"XM_006515051.2","start":1094,"end":1220,"product_size":127,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Taf1b","gene_name":"Taf1b"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"33990640a1","gene":"Acaca","target_transcript":"NM_133360.3","candidate_pairs_screened":10,"combined_pass_pairs":7,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GGTCTTCGAGTGGATTGGCA","right":"ATCCCTTTCCCTCCTCCTCC","transcript_product_size_bp":173,"primer3_penalty":0.066,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":33,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119745,"end":84122541,"product_size":2797,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":956,"end":1128,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1326,"end":1498,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1093,"end":1265,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1060,"end":1232,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1044,"end":1216,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1374,"end":1546,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1309,"end":1481,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1374,"end":1546,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1072,"end":1244,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1144,"end":1316,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1334,"end":1506,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1396,"end":1568,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGTCTTCGAGTGGATTGGC","right":"ATCCCTTTCCCTCCTCCTCC","transcript_product_size_bp":174,"primer3_penalty":0.066,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":32,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119744,"end":84122541,"product_size":2798,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":15,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":955,"end":1128,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1325,"end":1498,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1092,"end":1265,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1059,"end":1232,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1043,"end":1216,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1373,"end":1546,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1308,"end":1481,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1373,"end":1546,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1071,"end":1244,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1143,"end":1316,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1333,"end":1506,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1395,"end":1568,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCTGCTGGAGGACTTCATG","right":"ACCCCGAATAGACAGCTCCT","transcript_product_size_bp":124,"primer3_penalty":0.0677,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84136600,"end":84140279,"product_size":3680,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10202850,"end":10202973,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":1798,"end":1921,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2168,"end":2291,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1935,"end":2058,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1902,"end":2025,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1886,"end":2009,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2216,"end":2339,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2151,"end":2274,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2216,"end":2339,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1914,"end":2037,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1986,"end":2109,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2176,"end":2299,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2238,"end":2361,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"AGGAGCTGTCTATTCGGGGT","right":"CCAGCCAGTGTCGATTCTGT","transcript_product_size_bp":101,"primer3_penalty":0.069,"exon_span":{"spans_junction":false,"left_exon":14,"right_exon":14,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84140260,"end":84140360,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10202954,"end":10203054,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":1902,"end":2002,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2272,"end":2372,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":2039,"end":2139,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":2006,"end":2106,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1990,"end":2090,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2320,"end":2420,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2255,"end":2355,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2320,"end":2420,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":2018,"end":2118,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":2090,"end":2190,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2280,"end":2380,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2342,"end":2442,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":5,"left":"TGCCTCTGAGAACCCGAAAC","right":"GCCAATCCACTCGAAGACCA","transcript_product_size_bp":176,"primer3_penalty":0.0713,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115293,"end":84119763,"product_size":4471,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":799,"end":974,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1169,"end":1344,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":936,"end":1111,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":903,"end":1078,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":887,"end":1062,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1217,"end":1392,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1152,"end":1327,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1217,"end":1392,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":915,"end":1090,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":987,"end":1162,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1177,"end":1352,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1239,"end":1414,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCTCTGAGAACCCGAAAC","right":"TGCCAATCCACTCGAAGACC","transcript_product_size_bp":177,"primer3_penalty":0.0713,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115293,"end":84119764,"product_size":4472,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":799,"end":975,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1169,"end":1345,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":936,"end":1112,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":903,"end":1079,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":887,"end":1063,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1217,"end":1393,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1152,"end":1328,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1217,"end":1393,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":915,"end":1091,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":987,"end":1163,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1177,"end":1353,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1239,"end":1415,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCGAAACTCCCAGAACTGCT","right":"GCCAATCCACTCGAAGACCA","transcript_product_size_bp":163,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115306,"end":84119763,"product_size":4458,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":812,"end":974,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1182,"end":1344,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":949,"end":1111,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":916,"end":1078,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":900,"end":1062,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1230,"end":1392,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1165,"end":1327,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1230,"end":1392,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":928,"end":1090,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1000,"end":1162,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1190,"end":1352,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1252,"end":1414,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCGAAACTCCCAGAACTGCT","right":"TGCCAATCCACTCGAAGACC","transcript_product_size_bp":164,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115306,"end":84119764,"product_size":4459,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":812,"end":975,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1182,"end":1345,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":949,"end":1112,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":916,"end":1079,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":900,"end":1063,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1230,"end":1393,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1165,"end":1328,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1230,"end":1393,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":928,"end":1091,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1000,"end":1163,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1190,"end":1353,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1252,"end":1415,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GACATGTGTTTGCTGGCCAG","right":"ATCACACAGCCAGGGTCAAG","transcript_product_size_bp":133,"primer3_penalty":0.0762,"exon_span":{"spans_junction":true,"left_exon":18,"right_exon":19,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84150262,"end":84151544,"product_size":1283,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10203530,"end":10203662,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":16,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":2478,"end":2610,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2848,"end":2980,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":2615,"end":2747,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":2582,"end":2714,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":2566,"end":2698,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2896,"end":3028,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2831,"end":2963,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2896,"end":3028,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":2594,"end":2726,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":2666,"end":2798,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2856,"end":2988,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2918,"end":3050,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"GGTCTTCGAGTGGATTGGCA","right":"TTTCCCTCCTCCTCCCTCTG","transcript_product_size_bp":168,"primer3_penalty":0.0797,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":19,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119745,"end":84122536,"product_size":2792,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":956,"end":1123,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1326,"end":1493,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1093,"end":1260,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1060,"end":1227,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1044,"end":1211,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1374,"end":1541,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1309,"end":1476,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1374,"end":1541,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1072,"end":1239,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1144,"end":1311,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1334,"end":1501,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1396,"end":1563,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"33413406a1","gene":"Gpr18","target_transcript":"NM_182806.2","candidate_pairs_screened":10,"combined_pass_pairs":7,"transcript_gene_specific_pairs":7,"transcript_isoform_specific_pairs":7,"candidates":[{"rank":1,"left":"GCACAGAGACACCACCTACC","right":"TCTGAGAGGTGACCGTTTGC","transcript_product_size_bp":87,"primer3_penalty":0.0707,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122153109,"end":122153195,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":8,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":106,"end":192,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"},{"transcript_accession":"XR_001781471.2","start":1724,"end":1810,"product_size":87,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Gm46485","gene_name":"Gm46485"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":2,"left":"GAAGACCCAGACAAGGCCTC","right":"CACGTAGCACCCGATCATGA","transcript_product_size_bp":144,"primer3_penalty":0.1382,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149400,"end":122149543,"product_size":144,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":727,"end":870,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TCATGATCGGGTGCTACGTG","right":"AGATGTGGAAGGGCACGAAG","transcript_product_size_bp":147,"primer3_penalty":0.1383,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149273,"end":122149419,"product_size":147,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":851,"end":997,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GACACAGCACAGAGACACCA","right":"TCTGAGAGGTGACCGTTTGC","transcript_product_size_bp":93,"primer3_penalty":0.1384,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122153109,"end":122153201,"product_size":93,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":100,"end":192,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"},{"transcript_accession":"XR_001781471.2","start":1724,"end":1816,"product_size":93,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Gm46485","gene_name":"Gm46485"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"GAGCCTTCACCACCTTCCTC","right":"TGACCGTAAACTGCCCGATC","transcript_product_size_bp":164,"primer3_penalty":0.1444,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149058,"end":122149221,"product_size":164,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":8,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":1049,"end":1212,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCCTTCCACATCTGCTTT","right":"GAGGAAGGTGGTGAAGGCTC","transcript_product_size_bp":86,"primer3_penalty":0.1489,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149202,"end":122149287,"product_size":86,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":983,"end":1068,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CTTCCACATCTGCTTTGCCG","right":"GAGGAAGGTGGTGAAGGCTC","transcript_product_size_bp":82,"primer3_penalty":0.2056,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149202,"end":122149283,"product_size":82,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":987,"end":1068,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCTGCCTGAAGATCTCCGAC","right":"CACGTAGCACCCGATCATGA","transcript_product_size_bp":113,"primer3_penalty":0.2062,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149400,"end":122149512,"product_size":113,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":758,"end":870,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TCATGATCGGGTGCTACGTG","right":"CTTGTAGCATCAGGACGGCA","transcript_product_size_bp":171,"primer3_penalty":0.2105,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122149249,"end":122149419,"product_size":171,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":851,"end":1021,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGACCAACCCCACTATCGG","right":"TCTGAGAGGTGACCGTTTGC","transcript_product_size_bp":140,"primer3_penalty":0.2116,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_locus_accession":"NC_000080.7","target_locus_start":122148846,"target_locus_end":122153300,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":122153109,"end":122153248,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_182806.2","target_gene_id":"Gpr18","target_gene_name":"Gpr18","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_182806.2","start":53,"end":192,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr18","gene_name":"Gpr18"},{"transcript_accession":"XR_001781471.2","start":1724,"end":1863,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Gm46485","gene_name":"Gm46485"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"18558654a1","gene":"Vmn1r179","target_transcript":"NM_207545.1","candidate_pairs_screened":10,"combined_pass_pairs":1,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CCTGATGCTGGTGGTCACAT","right":"TGGGAAAGCCTGTGACCAAA","transcript_product_size_bp":131,"primer3_penalty":0.295,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628705,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":84,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":895,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":2,"left":"CCTGATGCTGGTGGTCACAT","right":"GGAAAGCCTGTGACCAAAGC","transcript_product_size_bp":129,"primer3_penalty":0.35,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628703,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":82,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":893,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":3,"left":"CCTGATGCTGGTGGTCACAT","right":"GTGGGAAAGCCTGTGACCAA","transcript_product_size_bp":132,"primer3_penalty":0.5021,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628706,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":83,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":896,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"ATCCTGATGCTGGTGGTCAC","right":"TGGGAAAGCCTGTGACCAAA","transcript_product_size_bp":133,"primer3_penalty":0.5144,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628573,"end":23628705,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":83,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":763,"end":895,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":5,"left":"ATCCTGATGCTGGTGGTCAC","right":"GGAAAGCCTGTGACCAAAGC","transcript_product_size_bp":131,"primer3_penalty":0.5694,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628573,"end":23628703,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":81,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":763,"end":893,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":6,"left":"ATCCTGATGCTGGTGGTCAC","right":"GTGGGAAAGCCTGTGACCAA","transcript_product_size_bp":134,"primer3_penalty":0.7215,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628573,"end":23628706,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":81,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":763,"end":896,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"CCTGATGCTGGTGGTCACAT","right":"TAGTGGGAAAGCCTGTGACC","transcript_product_size_bp":134,"primer3_penalty":0.7255,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628708,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":83,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":898,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":8,"left":"CCTGATGCTGGTGGTCACAT","right":"AGCTTCACTCCAATGCCTCA","transcript_product_size_bp":112,"primer3_penalty":0.7295,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628686,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":82,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":876,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":9,"left":"CCTGATGCTGGTGGTCACAT","right":"GCTTCACTCCAATGCCTCAC","transcript_product_size_bp":111,"primer3_penalty":0.8423,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"truncated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":65,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":true,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628575,"end":23628685,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"The alignment return limit was reached; no specificity pass can be assigned."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":82,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":765,"end":875,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"GACACTACAGAGGCCAAGCT","right":"TGGGAAAGCCTGTGACCAAA","transcript_product_size_bp":174,"primer3_penalty":0.8709,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_locus_accession":"NC_000073.7","target_locus_start":23627811,"target_locus_end":23628782,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":23628532,"end":23628705,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_207545.1","target_gene_id":"Vmn1r179","target_gene_name":"Vmn1r179","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_207545.1","start":722,"end":895,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r179","gene_name":"Vmn1r179"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"12832477a1","gene":"Acbd6","target_transcript":"NM_028250.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GAAAGCCCTCGGTGACTCAA","right":"CACCGAAACCACTACTGCCT","transcript_product_size_bp":131,"primer3_penalty":0.0698,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":815,"end":945,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":836,"end":966,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"AAAGCCCTCGGTGACTCAAG","right":"CACCGAAACCACTACTGCCT","transcript_product_size_bp":130,"primer3_penalty":0.0704,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":816,"end":945,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":837,"end":966,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GTTTGGCCGAGCTGTTTGAG","right":"TTGAGTCACCGAGGGCTTTC","transcript_product_size_bp":195,"primer3_penalty":0.075,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":640,"end":834,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":640,"end":834,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":640,"end":834,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":661,"end":855,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAGTTTGGCCGAGCTGTTTG","right":"TTGAGTCACCGAGGGCTTTC","transcript_product_size_bp":197,"primer3_penalty":0.075,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":638,"end":834,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":638,"end":834,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":638,"end":834,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":659,"end":855,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GTTTGGCCGAGCTGTTTGAG","right":"CTTGAGTCACCGAGGGCTTT","transcript_product_size_bp":196,"primer3_penalty":0.0755,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":640,"end":835,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":640,"end":835,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":640,"end":835,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":661,"end":856,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GAGTTTGGCCGAGCTGTTTG","right":"CTTGAGTCACCGAGGGCTTT","transcript_product_size_bp":198,"primer3_penalty":0.0755,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":638,"end":835,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":638,"end":835,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":638,"end":835,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":659,"end":856,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGGCAGTAGTGGTTTCGGTG","right":"TCACATCCGCAGCTTTCGAT","transcript_product_size_bp":149,"primer3_penalty":0.1431,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":926,"end":1074,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XM_006529883.5","start":144,"end":292,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CGAGCTAGTGAACATGGCCA","right":"CAAACAGCTCGGCCAAACTC","transcript_product_size_bp":152,"primer3_penalty":0.1483,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":155434371,"end":155434522,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":506,"end":657,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":506,"end":657,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":506,"end":657,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":527,"end":678,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CGAGCTAGTGAACATGGCCA","right":"CTCAAACAGCTCGGCCAAAC","transcript_product_size_bp":154,"primer3_penalty":0.1483,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":155434371,"end":155434524,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":506,"end":659,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":506,"end":659,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":506,"end":659,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":527,"end":680,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTGGCCGAGCTGTTTGAGAA","right":"TTGAGTCACCGAGGGCTTTC","transcript_product_size_bp":193,"primer3_penalty":0.2139,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_locus_accession":"NC_000067.7","target_locus_start":155433866,"target_locus_end":155562979,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028250.3","target_gene_id":"Acbd6","target_gene_name":"Acbd6","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028250.3","start":642,"end":834,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_001145781.1","start":642,"end":834,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"NM_026683.1","start":642,"end":834,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"},{"transcript_accession":"XR_387197.5","start":663,"end":855,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acbd6","gene_name":"Acbd6"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26327067a1","gene":"Fastkd3","target_transcript":"NM_001330423.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CCTCACACCTGACCAATGCT","right":"CACAACTCGCACCTTGAAGC","transcript_product_size_bp":195,"primer3_penalty":0.0783,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732189,"end":68732383,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":7,"right_hit_count":12,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":921,"end":1115,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":673,"end":867,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":673,"end":867,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":629,"end":823,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":928,"end":1122,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":629,"end":823,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTCAGCGCGAATTACCATGC","right":"GTGATGGGGAAAGGGAACGT","transcript_product_size_bp":109,"primer3_penalty":0.0796,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68730609,"end":68730717,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":281,"end":389,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":134,"end":242,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":288,"end":396,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":287,"end":395,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ACCTCCGTCCTAGAGTGTCC","right":"AGGGCGTCAACACCTTTGAA","transcript_product_size_bp":190,"primer3_penalty":0.1412,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733353,"end":68735938,"product_size":2586,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1910,"end":2099,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1662,"end":1851,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1662,"end":1851,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":419,"end":608,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1618,"end":1807,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":1071,"end":1260,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1166,"end":1355,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_017315595.2","start":869,"end":1058,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1917,"end":2106,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":1071,"end":1260,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":1027,"end":1216,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1319,"end":1508,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCCGTCCTAGAGTGTCCGTT","right":"AGGGCGTCAACACCTTTGAA","transcript_product_size_bp":187,"primer3_penalty":0.1442,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733356,"end":68735938,"product_size":2583,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1913,"end":2099,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1665,"end":1851,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1665,"end":1851,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":422,"end":608,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1621,"end":1807,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":1074,"end":1260,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1169,"end":1355,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_017315595.2","start":872,"end":1058,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1920,"end":2106,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":1074,"end":1260,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":1030,"end":1216,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1322,"end":1508,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGCTCCGCTTGCTTTTCTG","right":"GTGATGGGGAAAGGGAACGT","transcript_product_size_bp":157,"primer3_penalty":0.1476,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68730561,"end":68730717,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":233,"end":389,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":86,"end":242,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":240,"end":396,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":239,"end":395,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTGATTGAGCGACATCCCGT","right":"TGGGGCCCTTATAGAACGGA","transcript_product_size_bp":163,"primer3_penalty":0.21,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1784,"end":1946,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1536,"end":1698,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1536,"end":1698,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1492,"end":1654,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":945,"end":1107,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1040,"end":1202,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1791,"end":1953,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":945,"end":1107,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":901,"end":1063,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1193,"end":1355,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1492,"end":1654,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGCTCAATTACGTGCCACCA","right":"ACGGGATGTCGCTCAATCAG","transcript_product_size_bp":139,"primer3_penalty":0.2153,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732933,"end":68733071,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":11,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1665,"end":1803,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1417,"end":1555,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1417,"end":1555,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1373,"end":1511,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":826,"end":964,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":921,"end":1059,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1672,"end":1810,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":826,"end":964,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":782,"end":920,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1074,"end":1212,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1373,"end":1511,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTGATTGAGCGACATCCCGT","right":"AACGGACACTCTAGGACGGA","transcript_product_size_bp":149,"primer3_penalty":0.2164,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733052,"end":68733375,"product_size":324,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1784,"end":1932,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1536,"end":1684,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1536,"end":1684,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1492,"end":1640,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":945,"end":1093,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1040,"end":1188,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1791,"end":1939,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":945,"end":1093,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":901,"end":1049,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1193,"end":1341,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1492,"end":1640,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCTCCTTCATTCATGTGCGC","right":"TGGGGCCCTTATAGAACGGA","transcript_product_size_bp":182,"primer3_penalty":0.2793,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1765,"end":1946,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1517,"end":1698,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1517,"end":1698,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1473,"end":1654,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":926,"end":1107,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1021,"end":1202,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1772,"end":1953,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":926,"end":1107,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":882,"end":1063,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1174,"end":1355,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1473,"end":1654,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ATGCCAACGTCGTCTCCAAA","right":"TTCAGGGTCTCACAACTCGC","transcript_product_size_bp":102,"primer3_penalty":0.2836,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732292,"end":68732393,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":12,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1024,"end":1125,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":776,"end":877,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":776,"end":877,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":179,"end":280,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":732,"end":833,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":185,"end":286,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":280,"end":381,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1031,"end":1132,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":185,"end":286,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":141,"end":242,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":433,"end":534,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":732,"end":833,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13937369a1","gene":"Actr8","target_transcript":"NM_027493.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"ATTCCCCTGCCCTGCTTTAC","right":"GTGCTGCAGAGTGGTCATCT","transcript_product_size_bp":119,"primer3_penalty":0.0697,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29710956,"end":29711685,"product_size":730,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1241,"end":1359,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1236,"end":1354,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1236,"end":1354,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":952,"end":1070,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":961,"end":1079,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1236,"end":1354,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ATTCCCCTGCCCTGCTTTAC","right":"TCCCACGATCCCAAAGGTTG","transcript_product_size_bp":95,"primer3_penalty":0.0707,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29710956,"end":29711661,"product_size":706,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1241,"end":1335,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1236,"end":1330,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1236,"end":1330,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":952,"end":1046,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":961,"end":1055,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1236,"end":1330,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATTCCCCTGCCCTGCTTTAC","right":"GTCCCACGATCCCAAAGGTT","transcript_product_size_bp":96,"primer3_penalty":0.0708,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29710956,"end":29711662,"product_size":707,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1241,"end":1336,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1236,"end":1331,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1236,"end":1331,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":952,"end":1047,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":961,"end":1056,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1236,"end":1331,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"AAAGGAGGTGCAGTACTGGC","right":"CACACAAAGGCAGCTCGTTC","transcript_product_size_bp":113,"primer3_penalty":0.0778,"exon_span":{"spans_junction":false,"left_exon":12,"right_exon":12,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29714945,"end":29715057,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1888,"end":2000,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":3088,"end":3200,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1883,"end":1995,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":2804,"end":2916,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":1608,"end":1720,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGATCACAAGGCCCAAGGAC","right":"CACACAAAGGCAGCTCGTTC","transcript_product_size_bp":157,"primer3_penalty":0.0791,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":3,"right_hit_count":5,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1844,"end":2000,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1839,"end":1995,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":1564,"end":1720,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTGATCACAAGGCCCAAGGA","right":"CACACAAAGGCAGCTCGTTC","transcript_product_size_bp":158,"primer3_penalty":0.0791,"exon_span":{"spans_junction":true,"left_exon":11,"right_exon":12,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29713695,"end":29715057,"product_size":1363,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1843,"end":2000,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1838,"end":1995,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":1563,"end":1720,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1838,"end":3200,"product_size":1363,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":1554,"end":2916,"product_size":1363,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGTCTCATCGGAACACACGG","right":"TGGTCCTGAAGTCCGGAGAT","transcript_product_size_bp":196,"primer3_penalty":0.0799,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29709290,"end":29710932,"product_size":1643,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1022,"end":1217,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1017,"end":1212,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1017,"end":1212,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":733,"end":928,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":742,"end":937,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1017,"end":1212,"product_size":196,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TGTCTCATCGGAACACACGG","right":"ATGGTCCTGAAGTCCGGAGA","transcript_product_size_bp":197,"primer3_penalty":0.0799,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29709290,"end":29710933,"product_size":1644,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":6,"right_hit_count":8,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1022,"end":1218,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1017,"end":1213,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1017,"end":1213,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":733,"end":929,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":742,"end":938,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1017,"end":1213,"product_size":197,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GTGCAGTACTGGCGTGTTTG","right":"CACACAAAGGCAGCTCGTTC","transcript_product_size_bp":106,"primer3_penalty":0.0823,"exon_span":{"spans_junction":false,"left_exon":12,"right_exon":12,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29714952,"end":29715057,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":5,"right_hit_count":5,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":4,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1895,"end":2000,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":3095,"end":3200,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1890,"end":1995,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":2811,"end":2916,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":1615,"end":1720,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ATTCCCCTGCCCTGCTTTAC","right":"ATGGGGATCCTCAGGGTCTC","transcript_product_size_bp":149,"primer3_penalty":0.1378,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":9,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_locus_accession":"NC_000080.7","target_locus_start":29700294,"target_locus_end":29715178,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":29710956,"end":29711715,"product_size":760,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027493.3","target_gene_id":"Actr8","target_gene_name":"Actr8","left_hit_count":7,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027493.3","start":1241,"end":1389,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_006519309.4","start":1236,"end":1384,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247897.1","start":1236,"end":1384,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247898.2","start":952,"end":1100,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_030247899.2","start":961,"end":1109,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"},{"transcript_accession":"XM_036158765.1","start":1236,"end":1384,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Actr8","gene_name":"Actr8"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"29243196a1","gene":"Slc6a19","target_transcript":"NM_028878.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":6,"candidates":[{"rank":1,"left":"CCCCTGCTTTGCCATCTACA","right":"TCCACACACCATCTTCTGCC","transcript_product_size_bp":154,"primer3_penalty":0.0709,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":73829709,"end":73829862,"product_size":154,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":1938,"end":2091,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"},{"transcript_accession":"NM_001359603.1","start":1596,"end":1749,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCCCTGCTTTGCCATCTACA","right":"TGCTTCCTCCACACACCATC","transcript_product_size_bp":161,"primer3_penalty":0.0709,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":73829702,"end":73829862,"product_size":161,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":1938,"end":2098,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"},{"transcript_accession":"NM_001359603.1","start":1596,"end":1756,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AAGGCCCAGTACATGCTCAC","right":"CCTCTGACCGATGGCAAACT","transcript_product_size_bp":174,"primer3_penalty":0.0711,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":259,"end":432,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACAAGGCCCAGTACATGCTC","right":"CCTCTGACCGATGGCAAACT","transcript_product_size_bp":176,"primer3_penalty":0.0711,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":257,"end":432,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CAAGGCCCAGTACATGCTCA","right":"CCTCTGACCGATGGCAAACT","transcript_product_size_bp":175,"primer3_penalty":0.0712,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":258,"end":432,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AAGGCCCAGTACATGCTCAC","right":"TCTGACCGATGGCAAACTCC","transcript_product_size_bp":172,"primer3_penalty":0.0714,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":259,"end":430,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACAAGGCCCAGTACATGCTC","right":"TCTGACCGATGGCAAACTCC","transcript_product_size_bp":174,"primer3_penalty":0.0714,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":257,"end":430,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCCCTGCTTTGCCATCTACA","right":"CCACACACCATCTTCTGCCT","transcript_product_size_bp":153,"primer3_penalty":0.0715,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":73829710,"end":73829862,"product_size":153,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":1938,"end":2090,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"},{"transcript_accession":"NM_001359603.1","start":1596,"end":1748,"product_size":153,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGACATGTGCCTGGAGTGT","right":"GGGTTGCTCAGCTCTGTGAT","transcript_product_size_bp":194,"primer3_penalty":0.0715,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":5,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":73837257,"end":73837897,"product_size":641,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":748,"end":941,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"},{"transcript_accession":"NM_001359603.1","start":406,"end":599,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAAGGCCCAGTACATGCTCA","right":"TCTGACCGATGGCAAACTCC","transcript_product_size_bp":173,"primer3_penalty":0.0715,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_locus_accession":"NC_000079.7","target_locus_start":73827864,"target_locus_end":73848899,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_028878.4","target_gene_id":"Slc6a19","target_gene_name":"Slc6a19","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_028878.4","start":258,"end":430,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc6a19","gene_name":"Slc6a19"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26325068a1","gene":"Ide","target_transcript":"NM_031156.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GCTGATGACTGAAGTGGCCT","right":"CAATATGCAGCCGTGACAGC","transcript_product_size_bp":113,"primer3_penalty":0.0641,"exon_span":{"spans_junction":true,"left_exon":16,"right_exon":17,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37262637,"end":37264564,"product_size":1928,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":8,"right_hit_count":8,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2110,"end":2222,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2246,"end":2358,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":1974,"end":2086,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2149,"end":2261,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2210,"end":2322,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2008,"end":2120,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":3718,"end":3830,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GTGGACAGAAGGAAGGAGCC","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":141,"primer3_penalty":0.07,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37291450,"product_size":519,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1128,"end":1268,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1264,"end":1404,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":992,"end":1132,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1167,"end":1307,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1228,"end":1368,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":1026,"end":1166,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2736,"end":2876,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGTGGACAGAAGGAAGGAGC","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":142,"primer3_penalty":0.07,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37291451,"product_size":520,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1127,"end":1268,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1263,"end":1404,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":991,"end":1132,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1166,"end":1307,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1227,"end":1368,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":1025,"end":1166,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2735,"end":2876,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGTGCCCCTTGTTTGATGC","right":"GTTCCCCGTAGCCTTTTCCA","transcript_product_size_bp":123,"primer3_penalty":0.0751,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302578,"end":37302700,"product_size":123,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":676,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":812,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":540,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":715,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":776,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":574,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2284,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACCAGACAGACATGCAGAGC","right":"GCGCAGGGTATTGAAGCAAG","transcript_product_size_bp":89,"primer3_penalty":0.136,"exon_span":{"spans_junction":false,"left_exon":19,"right_exon":19,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37258000,"end":37258088,"product_size":89,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2430,"end":2518,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2566,"end":2654,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":2294,"end":2382,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2469,"end":2557,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2530,"end":2618,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2328,"end":2416,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":4038,"end":4126,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTCCGCTTGCTGATGACTGA","right":"CAATATGCAGCCGTGACAGC","transcript_product_size_bp":121,"primer3_penalty":0.137,"exon_span":{"spans_junction":true,"left_exon":16,"right_exon":17,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37262637,"end":37264572,"product_size":1936,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":8,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":2102,"end":2222,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":2238,"end":2358,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":1966,"end":2086,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":2141,"end":2261,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":2202,"end":2322,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":2000,"end":2120,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":3710,"end":3830,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGACAGGTTTGCGCAGTTTT","right":"GTTCCCCGTAGCCTTTTCCA","transcript_product_size_bp":145,"primer3_penalty":0.1395,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":532,"end":676,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":668,"end":812,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":396,"end":540,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":571,"end":715,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":632,"end":776,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":430,"end":574,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2140,"end":2284,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GTCCTGGAAGCCTGTTGTCA","right":"CTTGAGGTCCTTCTGCACGT","transcript_product_size_bp":198,"primer3_penalty":0.1426,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37290932,"end":37292268,"product_size":1337,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":1071,"end":1268,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":1207,"end":1404,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":935,"end":1132,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":1110,"end":1307,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":1171,"end":1368,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":969,"end":1166,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2679,"end":2876,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGTGCCCCTTGTTTGATGC","right":"CTGAAGGGGTGTTTGGGGTT","transcript_product_size_bp":140,"primer3_penalty":0.1437,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302561,"end":37302700,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":693,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":829,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":557,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":732,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":793,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":591,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2301,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTGTGCCCCTTGTTTGATGC","right":"TGAAGGGGTGTTTGGGGTTC","transcript_product_size_bp":139,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_locus_accession":"NC_000085.7","target_locus_start":37246142,"target_locus_end":37340056,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":37302562,"end":37302700,"product_size":139,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_031156.4","target_gene_id":"Ide","target_gene_name":"Ide","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_031156.4","start":554,"end":692,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161441.1","start":690,"end":828,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161442.1","start":418,"end":556,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161443.1","start":593,"end":731,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161444.1","start":654,"end":792,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161445.1","start":452,"end":590,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"},{"transcript_accession":"XM_036161446.1","start":2162,"end":2300,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Ide","gene_name":"Ide"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31543728a1","gene":"Slc2a5","target_transcript":"NM_019741.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CCCTCAGAGTTCATGCAGCA","right":"GCGTCAAGGTGAAGGACTCA","transcript_product_size_bp":82,"primer3_penalty":0.0692,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":774,"end":855,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":244,"end":325,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GAGGATGAGGCTGAGAAGGC","right":"CCTGCGCTGAGGTAGATCTG","transcript_product_size_bp":167,"primer3_penalty":0.1384,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":6,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150224147,"end":150224424,"product_size":278,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":30,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1407,"end":1573,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":877,"end":1043,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATGGGTACAACGTAGCTGCC","right":"GCGTCAAGGTGAAGGACTCA","transcript_product_size_bp":111,"primer3_penalty":0.1414,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150210108,"end":150210655,"product_size":548,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":745,"end":855,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":215,"end":325,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGTTTGGCCTTCGGAGTCTC","right":"AAAGAACGGGAGGAGGAGGA","transcript_product_size_bp":104,"primer3_penalty":0.1514,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":40,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150220118,"end":150223945,"product_size":3828,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1186,"end":1289,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":656,"end":759,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGTTTGGCCTTCGGAGTCT","right":"AAAGAACGGGAGGAGGAGGA","transcript_product_size_bp":105,"primer3_penalty":0.152,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":40,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150220117,"end":150223945,"product_size":3829,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1185,"end":1289,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":655,"end":759,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GGTCCCGCAACTCTTCATCA","right":"AAAGAACGGGAGGAGGAGGA","transcript_product_size_bp":148,"primer3_penalty":0.1529,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":40,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150220074,"end":150223945,"product_size":3872,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1142,"end":1289,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":612,"end":759,"product_size":148,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGGTCCCGCAACTCTTCATC","right":"AAAGAACGGGAGGAGGAGGA","transcript_product_size_bp":149,"primer3_penalty":0.1529,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":40,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150220073,"end":150223945,"product_size":3873,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":6,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1141,"end":1289,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":611,"end":759,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TATGCAGTCTCTCCGTTGGC","right":"CCTGCGCTGAGGTAGATCTG","transcript_product_size_bp":114,"primer3_penalty":0.2076,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150224200,"end":150224424,"product_size":225,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1460,"end":1573,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":930,"end":1043,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCTGCAGAACACCATCTCCT","right":"GACACTGCCTCCGATCATGT","transcript_product_size_bp":163,"primer3_penalty":0.2123,"exon_span":{"spans_junction":true,"left_exon":8,"right_exon":10,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1742,"end":1904,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":1212,"end":1374,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ACATGATCGGAGGCAGTGTC","right":"TGAATGTCCTGCCCTTGGTC","transcript_product_size_bp":171,"primer3_penalty":0.2148,"exon_span":{"spans_junction":false,"left_exon":10,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_locus_accession":"NC_000070.7","target_locus_start":150203801,"target_locus_end":150228625,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":150227317,"end":150227584,"product_size":268,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019741.3","target_gene_id":"Slc2a5","target_gene_name":"Slc2a5","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019741.3","start":1885,"end":2055,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc2a5","gene_name":"Slc2a5"},{"transcript_accession":"XM_006539076.5","start":1355,"end":1525,"product_size":171,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc2a5","gene_name":"Slc2a5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"29611455a1","gene":"Gpr137c","target_transcript":"NM_027518.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GGGAACTCACGGGAAGGAAG","right":"GGGGTGTGACTGTGTAGGTG","transcript_product_size_bp":97,"primer3_penalty":0.0712,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45516488,"end":45517436,"product_size":949,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1351,"end":1447,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1110,"end":1206,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":2032,"end":2128,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1504,"end":1600,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCTTCGCCTTTGCCTACCTG","right":"CGAGACACAGGAAGAGGCAG","transcript_product_size_bp":93,"primer3_penalty":0.1453,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45457610,"end":45457702,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":437,"end":529,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316190.2","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316191.3","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316192.2","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":469,"end":561,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ACGGTATGACAGTGACGACG","right":"GGGGTGTGACTGTGTAGGTG","transcript_product_size_bp":131,"primer3_penalty":0.2024,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45516454,"end":45517436,"product_size":983,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1317,"end":1447,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1076,"end":1206,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":1998,"end":2128,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1470,"end":1600,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACGGTATGACAGTGACGACG","right":"GTACCAGCCCAAAGTTTGCG","transcript_product_size_bp":85,"primer3_penalty":0.207,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45516454,"end":45517390,"product_size":937,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1317,"end":1401,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1076,"end":1160,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":1998,"end":2082,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1470,"end":1554,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"ACGGTATGACAGTGACGACG","right":"CGTACCAGCCCAAAGTTTGC","transcript_product_size_bp":86,"primer3_penalty":0.207,"exon_span":{"spans_junction":false,"left_exon":4,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45516454,"end":45517391,"product_size":938,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1317,"end":1402,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1076,"end":1161,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":1998,"end":2083,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1470,"end":1555,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCTTCGCCTTTGCCTACCTG","right":"GAGAAGAGCGTGGTCCTGAG","transcript_product_size_bp":124,"primer3_penalty":0.2087,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45457610,"end":45457733,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":8,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":437,"end":560,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316190.2","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316191.3","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316192.2","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":469,"end":592,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGCTTTCCTACGGCTGAAGT","right":"TGCAGAGGTCAAAACACGGA","transcript_product_size_bp":160,"primer3_penalty":0.2155,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45517539,"end":45517698,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1550,"end":1709,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1309,"end":1468,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":2231,"end":2390,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1703,"end":1862,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TGGCTTTCCTACGGCTGAAG","right":"TGCAGAGGTCAAAACACGGA","transcript_product_size_bp":161,"primer3_penalty":0.2156,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45517538,"end":45517698,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":1549,"end":1709,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":1308,"end":1468,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":2230,"end":2390,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":1702,"end":1862,"product_size":161,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GTTCTCCACGCTCTGTCTCC","right":"CTCCGTGGACTAGCATTGCA","transcript_product_size_bp":167,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":7,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":663,"end":829,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":695,"end":861,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":695,"end":861,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTGCCTCTTCCTGTGTCTCG","right":"GGAGACAGAGCGTGGAGAAC","transcript_product_size_bp":173,"primer3_penalty":0.2179,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_locus_accession":"NC_000080.7","target_locus_start":45457174,"target_locus_end":45518433,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":45457683,"end":45457855,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_027518.2","target_gene_id":"Gpr137c","target_gene_name":"Gpr137c","left_hit_count":7,"right_hit_count":8,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_027518.2","start":510,"end":682,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_006519544.4","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316190.2","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316191.3","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XM_017316192.2","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_004938445.1","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"},{"transcript_accession":"XR_874367.3","start":542,"end":714,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Gpr137c","gene_name":"Gpr137c"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31982010a1","gene":"Slc22a1","target_transcript":"NM_009202.5","candidate_pairs_screened":10,"combined_pass_pairs":9,"transcript_gene_specific_pairs":9,"transcript_isoform_specific_pairs":9,"candidates":[{"rank":1,"left":"CCTTCACAGTGGGGCTAGTG","right":"CAGCAGGAAGAGGAAGGTGG","transcript_product_size_bp":104,"primer3_penalty":0.0716,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":13,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12883285,"end":12883388,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":1,"right_hit_count":8,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":910,"end":1013,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCACCTTCCTCTTCCTGCTG","right":"CATCTTCAGGTCAGCAGGGG","transcript_product_size_bp":146,"primer3_penalty":0.1429,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":5,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":13,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":9,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":994,"end":1139,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGTGAACGCTGAGCTGTACC","right":"TGCCAAACTTCCATCAGCCT","transcript_product_size_bp":123,"primer3_penalty":0.1512,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12871282,"end":12875857,"product_size":4576,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":1535,"end":1657,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGGAGCACGTTGGAGAGTT","right":"GAAGCCCAGGAAAACGATGC","transcript_product_size_bp":111,"primer3_penalty":0.2053,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12894415,"end":12894525,"product_size":111,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":201,"end":311,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AACCAGAGCACCCTTGACTG","right":"CTCAGTGACGATGGAGGAGC","transcript_product_size_bp":126,"primer3_penalty":0.2115,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12894133,"end":12894258,"product_size":126,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000083.7","start":12803456,"end":12803581,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":468,"end":593,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"},{"transcript_accession":"NM_001355767.1","start":381,"end":506,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc22a2","gene_name":"Slc22a2"},{"transcript_accession":"NM_013667.3","start":381,"end":506,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Slc22a2","gene_name":"Slc22a2"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"GCTCCTCCATCGTCACTGAG","right":"CCCAGGAAGAAGCCCAAGTT","transcript_product_size_bp":94,"primer3_penalty":0.2153,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":574,"end":667,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGTGAACGCTGAGCTGTACC","right":"AGGGCTTGCCAAACTTCCAT","transcript_product_size_bp":129,"primer3_penalty":0.2171,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12871276,"end":12875857,"product_size":4582,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":1535,"end":1663,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ATCTCGGGATGATGGTGTGC","right":"TGCCAAACTTCCATCAGCCT","transcript_product_size_bp":88,"primer3_penalty":0.2205,"exon_span":{"spans_junction":false,"left_exon":8,"right_exon":8,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12871282,"end":12871369,"product_size":88,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":1570,"end":1657,"product_size":88,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AACCAGAGCACCCTTGACTG","right":"CCCAGGAAGAAGCCCAAGTT","transcript_product_size_bp":200,"primer3_penalty":0.2225,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":468,"end":667,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCCCTGTCTGGCGTGTTAAC","right":"CCTTTGCTGACCATGCCTTG","transcript_product_size_bp":86,"primer3_penalty":0.2799,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_locus_accession":"NC_000083.7","target_locus_start":12867761,"target_locus_end":12894725,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":12885538,"end":12885623,"product_size":86,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_009202.5","target_gene_id":"Slc22a1","target_gene_name":"Slc22a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_009202.5","start":741,"end":826,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a1","gene_name":"Slc22a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"13562102a1","gene":"Prrt1","target_transcript":"NM_001368729.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GACCACCATCTGCTGCTTCT","right":"TCGGCAGACACCAAATCTCC","transcript_product_size_bp":99,"primer3_penalty":0.072,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34850872,"end":34851098,"product_size":227,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":486,"end":584,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":947,"end":1045,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":270,"end":368,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":809,"end":907,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1438,"end":1536,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":918,"end":1016,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1241,"end":1339,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ATCCTCCAGTCGCCCTAGTT","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":94,"primer3_penalty":0.0733,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851466,"end":34851559,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":952,"end":1045,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1413,"end":1506,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":736,"end":829,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1275,"end":1368,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1904,"end":1997,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1384,"end":1477,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1707,"end":1800,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AATCCTCCAGTCGCCCTAGT","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":95,"primer3_penalty":0.0733,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851465,"end":34851559,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":951,"end":1045,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1412,"end":1506,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":735,"end":829,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1274,"end":1368,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1903,"end":1997,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1383,"end":1477,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1706,"end":1800,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GGAGATTTGGTGTCTGCCGA","right":"GGCGGCGATGATGATGACTA","transcript_product_size_bp":126,"primer3_penalty":0.0746,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851079,"end":34851204,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":565,"end":690,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1026,"end":1151,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":349,"end":474,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":888,"end":1013,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1517,"end":1642,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":997,"end":1122,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1320,"end":1445,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCACACGACTACATGCCCAT","right":"TCGGCAGACACCAAATCTCC","transcript_product_size_bp":128,"primer3_penalty":0.1441,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34850843,"end":34851098,"product_size":256,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":457,"end":584,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":918,"end":1045,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":241,"end":368,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":780,"end":907,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1409,"end":1536,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":889,"end":1016,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1212,"end":1339,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTCCAGTCGCCCTAGTTTCC","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":91,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851469,"end":34851559,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":955,"end":1045,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1416,"end":1506,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":739,"end":829,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1278,"end":1368,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1907,"end":1997,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1387,"end":1477,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1710,"end":1800,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTCCAGTCGCCCTAGTTTC","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":92,"primer3_penalty":0.2172,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851468,"end":34851559,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":10,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":954,"end":1045,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1415,"end":1506,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":738,"end":829,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1277,"end":1368,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1906,"end":1997,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1386,"end":1477,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1709,"end":1800,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCCAGCTCACATACAGACGG","right":"CCAAAAGCAGCAAAACGGGA","transcript_product_size_bp":178,"primer3_penalty":0.283,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851685,"end":34851862,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":1171,"end":1348,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1632,"end":1809,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":955,"end":1132,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1494,"end":1671,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":2123,"end":2300,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1603,"end":1780,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1926,"end":2103,"product_size":178,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TAACACCCACATCCTCAGCG","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":139,"primer3_penalty":0.2904,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851421,"end":34851559,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":907,"end":1045,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1368,"end":1506,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":691,"end":829,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1230,"end":1368,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1859,"end":1997,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1339,"end":1477,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1662,"end":1800,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCCTCCAGTCGCCCTAGTTT","right":"TGGGACCTGACTCCTACTGG","transcript_product_size_bp":93,"primer3_penalty":0.2933,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_locus_accession":"NC_000083.7","target_locus_start":34848538,"target_locus_end":34852100,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000083.7","start":34851467,"end":34851559,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001368729.1","target_gene_id":"Prrt1","target_gene_name":"Prrt1","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001368729.1","start":953,"end":1045,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368728.1","start":1414,"end":1506,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_001368730.1","start":737,"end":829,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"NM_030890.2","start":1276,"end":1368,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_011246470.4","start":1905,"end":1997,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_030249815.1","start":1385,"end":1477,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"},{"transcript_accession":"XM_036160615.1","start":1708,"end":1800,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Prrt1","gene_name":"Prrt1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"2852697a1","gene":"Akap2","target_transcript":"NM_001384157.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GCAGGAAATACAAGCGCTCG","right":"TAGACCACCCAGATGCTCCA","transcript_product_size_bp":146,"primer3_penalty":0.0694,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":5,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":347,"end":492,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":387,"end":532,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":379,"end":524,"product_size":146,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGAATATCAGGCTGGGCTCC","right":"CTGAGGTGGTGCAAACATGC","transcript_product_size_bp":187,"primer3_penalty":0.0763,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57856430,"end":57856616,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":2429,"end":2615,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1928,"end":2114,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1869,"end":2055,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":2469,"end":2655,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":2461,"end":2647,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1928,"end":2114,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCAGGAAATACAAGCGCTCG","right":"GCAACACAGTAGACCACCCA","transcript_product_size_bp":155,"primer3_penalty":0.1346,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":347,"end":501,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":387,"end":541,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":379,"end":533,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCAGCTCTCTCTCCCCAGAT","right":"GAGTGGTCCTTGGAATGGGG","transcript_product_size_bp":106,"primer3_penalty":0.1396,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57854644,"end":57854749,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":643,"end":748,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":142,"end":247,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":83,"end":188,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":683,"end":788,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":675,"end":780,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":142,"end":247,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AAGCCACCGACCATCTTGAG","right":"TAGATAAGGGCCCGCGTTTC","transcript_product_size_bp":190,"primer3_penalty":0.1414,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57855831,"end":57856020,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":1830,"end":2019,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1329,"end":1518,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1270,"end":1459,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":1870,"end":2059,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":1862,"end":2051,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1329,"end":1518,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AAGCCACCGACCATCTTGAG","right":"AGTTTAGATAAGGGCCCGCG","transcript_product_size_bp":194,"primer3_penalty":0.1422,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57855831,"end":57856024,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":1830,"end":2023,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1329,"end":1522,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1270,"end":1463,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":1870,"end":2063,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":1862,"end":2055,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1329,"end":1522,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACTGAGGTCAAGGAAGCAGC","right":"TCACTTGTCTCTGCCGCTTT","transcript_product_size_bp":98,"primer3_penalty":0.1427,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57856862,"end":57856959,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":2861,"end":2958,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":2360,"end":2457,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":2301,"end":2398,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":2901,"end":2998,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":2893,"end":2990,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":2360,"end":2457,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGAGGATGTGGTCACTGAGC","right":"CTCAAGATGGTCGGTGGCTT","transcript_product_size_bp":177,"primer3_penalty":0.144,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57855674,"end":57855850,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":11,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":1673,"end":1849,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1172,"end":1348,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1113,"end":1289,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":1713,"end":1889,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":1705,"end":1881,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1172,"end":1348,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAAACAGACAACCCCTCCGA","right":"GGAGCCCAGCCTGATATTCC","transcript_product_size_bp":133,"primer3_penalty":0.1453,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57856317,"end":57856449,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":2316,"end":2448,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1815,"end":1947,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1756,"end":1888,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":2356,"end":2488,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":2348,"end":2480,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1815,"end":1947,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTCAGGAAAACTCGCTGGCT","right":"GGAGCCCAGCCTGATATTCC","transcript_product_size_bp":177,"primer3_penalty":0.146,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_locus_accession":"NC_000070.7","target_locus_start":57434475,"target_locus_end":57896977,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000070.7","start":57856273,"end":57856449,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001384157.1","target_gene_id":"Pakap","target_gene_name":"Pakap","left_hit_count":6,"right_hit_count":6,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001384157.1","start":2272,"end":2448,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035532.2","start":1771,"end":1947,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001035533.2","start":1712,"end":1888,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001304544.1","start":2312,"end":2488,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_001384150.1","start":2304,"end":2480,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"},{"transcript_accession":"NM_009649.3","start":1771,"end":1947,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Pakap","gene_name":"Pakap"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"27370266a1","gene":"Slco5a1","target_transcript":"XM_011238381.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TCGCTGCTTCTTAGTGTGCA","right":"TAGCTGACGAACACCACCAC","transcript_product_size_bp":180,"primer3_penalty":0.0663,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060151,"end":13060330,"product_size":180,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1098,"end":1277,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1030,"end":1209,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1103,"end":1282,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1150,"end":1329,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1150,"end":1329,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2360,"end":2539,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1103,"end":1282,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1082,"end":1261,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1075,"end":1254,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TCGCTGCTTCTTAGTGTGCA","right":"GTAGCTGACGAACACCACCA","transcript_product_size_bp":181,"primer3_penalty":0.0663,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060150,"end":13060330,"product_size":181,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1098,"end":1278,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1030,"end":1210,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1103,"end":1283,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1150,"end":1330,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1150,"end":1330,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2360,"end":2540,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1103,"end":1283,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1082,"end":1262,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1075,"end":1255,"product_size":181,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGTGGGCTACTCATTGGCTT","right":"TCTTGTGTCGAGGTGGAAGC","transcript_product_size_bp":175,"primer3_penalty":0.0676,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13009333,"end":13014286,"product_size":4954,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":11,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1675,"end":1849,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1680,"end":1781,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1652,"end":1753,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1607,"end":1781,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1727,"end":1901,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1727,"end":1901,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2937,"end":3111,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCTTACGGATTCTCGCTGCT","right":"TAGCTGACGAACACCACCAC","transcript_product_size_bp":192,"primer3_penalty":0.1362,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060151,"end":13060342,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1086,"end":1277,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1018,"end":1209,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1091,"end":1282,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1138,"end":1329,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1138,"end":1329,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2348,"end":2539,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1091,"end":1282,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1070,"end":1261,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1063,"end":1254,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCTTACGGATTCTCGCTGCT","right":"GTAGCTGACGAACACCACCA","transcript_product_size_bp":193,"primer3_penalty":0.1362,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13060150,"end":13060342,"product_size":193,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1086,"end":1278,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1018,"end":1210,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1091,"end":1283,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1138,"end":1330,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1138,"end":1330,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2348,"end":2540,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1091,"end":1283,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1070,"end":1262,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1063,"end":1255,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ACCTTGACCAGAGTGACCCT","right":"TCTTGTGTCGAGGTGGAAGC","transcript_product_size_bp":129,"primer3_penalty":0.1384,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":13009333,"end":13014240,"product_size":4908,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":11,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1721,"end":1849,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1726,"end":1781,"product_size":56,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1698,"end":1753,"product_size":56,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1653,"end":1781,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1773,"end":1901,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1773,"end":1901,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2983,"end":3111,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGCTGGGAGTACAACGTGAC","right":"CCCACTTGCTCACTCACAGT","transcript_product_size_bp":179,"primer3_penalty":0.1404,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12942288,"end":12942466,"product_size":179,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2887,"end":3065,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2819,"end":2997,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2819,"end":2997,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2939,"end":3117,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2774,"end":2952,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3984,"end":4162,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":2680,"end":2858,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":2659,"end":2837,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2791,"end":2969,"product_size":179,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCGATTTACACCCTGGGACC","right":"AAGCCAATGAGTAGCCCACC","transcript_product_size_bp":140,"primer3_penalty":0.1415,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":9,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":1555,"end":1694,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":1487,"end":1626,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":1560,"end":1699,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":1607,"end":1746,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":1607,"end":1746,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":2817,"end":2956,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1560,"end":1699,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1539,"end":1678,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":1532,"end":1671,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TCATCATTGTCCCCAGTGCC","right":"GAACAGGGTGGAGAAGCACA","transcript_product_size_bp":140,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12964707,"end":12964846,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":12,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2138,"end":2277,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2070,"end":2209,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2070,"end":2209,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2190,"end":2329,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2025,"end":2164,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3235,"end":3374,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1931,"end":2070,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1910,"end":2049,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2042,"end":2181,"product_size":140,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCATCATTGTCCCCAGTGCC","right":"TGAACAGGGTGGAGAAGCAC","transcript_product_size_bp":141,"primer3_penalty":0.1428,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_locus_accession":"NC_000067.7","target_locus_start":12936773,"target_locus_end":13062689,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":12964706,"end":12964846,"product_size":141,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"XM_011238381.4","target_gene_id":"Slco5a1","target_gene_name":"Slco5a1","left_hit_count":11,"right_hit_count":9,"paired_amplicon_count":9,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":8,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"XM_011238381.4","start":2138,"end":2278,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"NM_172841.2","start":2070,"end":2210,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_006495518.4","start":2070,"end":2210,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238380.4","start":2190,"end":2330,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_011238382.4","start":2025,"end":2165,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253981.2","start":3235,"end":3375,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253982.1","start":1931,"end":2071,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253983.2","start":1910,"end":2050,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"},{"transcript_accession":"XM_030253984.2","start":2042,"end":2182,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slco5a1","gene_name":"Slco5a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26324632a1","gene":"Mob1a","target_transcript":"NM_145571.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TTTGACTCCGTGATGCAGCT","right":"TCCTGAAGTGGTGCCAACTC","transcript_product_size_bp":119,"primer3_penalty":0.1439,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315352,"end":83317201,"product_size":1850,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":707,"end":825,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":745,"end":863,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1142,"end":1260,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":612,"end":730,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1137,"end":1255,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":479,"end":597,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":716,"end":834,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TTTGACTCCGTGATGCAGCT","right":"CCTGAAGTGGTGCCAACTCA","transcript_product_size_bp":118,"primer3_penalty":0.2143,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315352,"end":83317200,"product_size":1849,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":707,"end":824,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":745,"end":862,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1142,"end":1259,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":612,"end":729,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1137,"end":1254,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":479,"end":596,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":716,"end":833,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GCACTTTGACTCCGTGATGC","right":"TCCTGAAGTGGTGCCAACTC","transcript_product_size_bp":123,"primer3_penalty":0.2754,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315348,"end":83317201,"product_size":1854,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":9,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":703,"end":825,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":741,"end":863,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1138,"end":1260,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":608,"end":730,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1133,"end":1255,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":475,"end":597,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":712,"end":834,"product_size":123,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACCACCAGCACTTTGACTCC","right":"TCCTGAAGTGGTGCCAACTC","transcript_product_size_bp":130,"primer3_penalty":0.2876,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315341,"end":83317201,"product_size":1861,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":696,"end":825,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":734,"end":863,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1131,"end":1260,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":601,"end":730,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1126,"end":1255,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":468,"end":597,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":705,"end":834,"product_size":130,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GCACTTTGACTCCGTGATGC","right":"CCTGAAGTGGTGCCAACTCA","transcript_product_size_bp":122,"primer3_penalty":0.3458,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315348,"end":83317200,"product_size":1853,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":703,"end":824,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":741,"end":862,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1138,"end":1259,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":608,"end":729,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1133,"end":1254,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":475,"end":596,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":712,"end":833,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"ACCACCAGCACTTTGACTCC","right":"CCTGAAGTGGTGCCAACTCA","transcript_product_size_bp":129,"primer3_penalty":0.358,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315341,"end":83317200,"product_size":1860,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":696,"end":824,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":734,"end":862,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1131,"end":1259,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":601,"end":729,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1126,"end":1254,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":468,"end":596,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":705,"end":833,"product_size":129,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCAGCACTTTGACTCCGTGA","right":"TCCTGAAGTGGTGCCAACTC","transcript_product_size_bp":126,"primer3_penalty":0.3585,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315345,"end":83317201,"product_size":1857,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":700,"end":825,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":738,"end":863,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1135,"end":1260,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":605,"end":730,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1130,"end":1255,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":472,"end":597,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":709,"end":834,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TTTGACTCCGTGATGCAGCT","right":"TGCCAACTCACGCCTATCAA","transcript_product_size_bp":108,"primer3_penalty":0.3586,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315352,"end":83317190,"product_size":1839,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":707,"end":814,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":745,"end":852,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1142,"end":1249,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":612,"end":719,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1137,"end":1244,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":479,"end":586,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":716,"end":823,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TTTGACTCCGTGATGCAGCT","right":"GTGCCAACTCACGCCTATCA","transcript_product_size_bp":109,"primer3_penalty":0.4264,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315352,"end":83317191,"product_size":1840,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":707,"end":815,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":745,"end":853,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1142,"end":1250,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":612,"end":720,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1137,"end":1245,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":479,"end":587,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":716,"end":824,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CCAGCACTTTGACTCCGTGA","right":"CCTGAAGTGGTGCCAACTCA","transcript_product_size_bp":125,"primer3_penalty":0.4289,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_locus_accession":"NC_000072.7","target_locus_start":83303028,"target_locus_end":83320758,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000072.7","start":83315345,"end":83317200,"product_size":1856,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145571.3","target_gene_id":"Mob1a","target_gene_name":"Mob1a","left_hit_count":7,"right_hit_count":7,"paired_amplicon_count":7,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":6,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145571.3","start":700,"end":824,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418174.1","start":738,"end":862,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418175.1","start":1135,"end":1259,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418176.1","start":605,"end":729,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418177.1","start":1130,"end":1254,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"NM_001418178.1","start":472,"end":596,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"},{"transcript_accession":"XM_006505969.4","start":709,"end":833,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Mob1a","gene_name":"Mob1a"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21717789a1","gene":"Vmn1r216","target_transcript":"NM_134245.1","candidate_pairs_screened":10,"combined_pass_pairs":1,"transcript_gene_specific_pairs":9,"transcript_isoform_specific_pairs":9,"candidates":[{"rank":1,"left":"CTACCATCCAAGCCCACAGT","right":"CCATTGACCCACTGCTCCAT","transcript_product_size_bp":100,"primer3_penalty":0.6544,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283823,"end":23283922,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241396,"end":23241494,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":505,"end":604,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":2,"left":"TGTCACCATCAGTCCCAGGA","right":"ACTGTGGGCTTGGATGGTAG","transcript_product_size_bp":198,"primer3_penalty":0.7995,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":36,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283645,"end":23283842,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241206,"end":23241415,"product_size":210,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":12,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":327,"end":524,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":3,"left":"CTACCATCCAAGCCCACAGT","right":"CCACTGCTCCATCCCATGAG","transcript_product_size_bp":92,"primer3_penalty":0.7996,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":18,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283823,"end":23283914,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241396,"end":23241486,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":13,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":505,"end":596,"product_size":92,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"CTACCATCCAAGCCCACAGT","right":"AACCATTGACCCACTGCTCC","transcript_product_size_bp":102,"primer3_penalty":0.8719,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283823,"end":23283924,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":505,"end":606,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGTCACCATCAGTCCCAGG","right":"ACTGTGGGCTTGGATGGTAG","transcript_product_size_bp":199,"primer3_penalty":0.8719,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":26,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283644,"end":23283842,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241205,"end":23241415,"product_size":211,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":10,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":326,"end":524,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":6,"left":"CTACCATCCAAGCCCACAGT","right":"ATTGACCCACTGCTCCATCC","transcript_product_size_bp":98,"primer3_penalty":0.8776,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283823,"end":23283920,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241396,"end":23241492,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":505,"end":602,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":7,"left":"CTACCATCCAAGCCCACAGTT","right":"CCATTGACCCACTGCTCCAT","transcript_product_size_bp":100,"primer3_penalty":1.0385,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283823,"end":23283922,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241396,"end":23241494,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":505,"end":604,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":8,"left":"ACCATCCAAGCCCACAGTTA","right":"CCATTGACCCACTGCTCCAT","transcript_product_size_bp":98,"primer3_penalty":1.1061,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283825,"end":23283922,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241398,"end":23241494,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":507,"end":604,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":9,"left":"TACCATCCAAGCCCACAGTT","right":"CCATTGACCCACTGCTCCAT","transcript_product_size_bp":99,"primer3_penalty":1.1061,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283824,"end":23283922,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241397,"end":23241494,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":506,"end":604,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"CTCATGGGATGGAGCAGTGG","right":"CAGACCTGGAGCTGTGAAGA","transcript_product_size_bp":85,"primer3_penalty":1.1512,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_locus_accession":"NC_000079.7","target_locus_start":23283319,"target_locus_end":23284215,"target_locus_strand":"+","left_hit_count":18,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":23283895,"end":23283979,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":23241467,"end":23241551,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22328846,"end":22328930,"product_size":85,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22659370,"end":22659454,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":23434173,"end":23434257,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_134245.1","target_gene_id":"Vmn1r216","target_gene_name":"Vmn1r216","left_hit_count":13,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_134245.1","start":577,"end":661,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r216","gene_name":"Vmn1r216"},{"transcript_accession":"NM_001083311.1","start":766,"end":850,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r223","gene_name":"Vmn1r223"},{"transcript_accession":"NM_134221.1","start":583,"end":667,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r201","gene_name":"Vmn1r201"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false}]},{"primerbank_id":"33468919a1","gene":"Olfr56","target_transcript":"NM_010999.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CCTTTTCGACACCCTGCTCT","right":"ATGAACATTGCTGCCCCGTA","transcript_product_size_bp":195,"primer3_penalty":0.07,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025602,"end":49025796,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":603,"end":797,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCTTTTCGACACCCTGCTCT","right":"AACATTGCTGCCCCGTAGAA","transcript_product_size_bp":192,"primer3_penalty":0.073,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025602,"end":49025793,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":603,"end":794,"product_size":192,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TTCCTGGGCTTTTGGGATCC","right":"TCGAAAAGGGAGGTGTCTGC","transcript_product_size_bp":147,"primer3_penalty":0.076,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025464,"end":49025610,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":465,"end":611,"product_size":147,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTCCTGGGCTTTTGGGATCC","right":"AGAGCAGGGTGTCGAAAAGG","transcript_product_size_bp":158,"primer3_penalty":0.0765,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025464,"end":49025621,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":465,"end":622,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCTGGGCTTTTGGGATCCTT","right":"TCGAAAAGGGAGGTGTCTGC","transcript_product_size_bp":145,"primer3_penalty":0.0766,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025466,"end":49025610,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":467,"end":611,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CCTGGGCTTTTGGGATCCTT","right":"AGAGCAGGGTGTCGAAAAGG","transcript_product_size_bp":156,"primer3_penalty":0.0771,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025466,"end":49025621,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":467,"end":622,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCTTTTCGACACCCTGCTCT","right":"CATTGCTGCCCCGTAGAAGA","transcript_product_size_bp":190,"primer3_penalty":0.1432,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025602,"end":49025791,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":603,"end":792,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"ACACCTCCCTTTTCGACACC","right":"AACATTGCTGCCCCGTAGAA","transcript_product_size_bp":199,"primer3_penalty":0.1456,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025595,"end":49025793,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":596,"end":794,"product_size":199,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCCACCCACTTCACTATCCC","right":"AAGGATCCCAAAAGCCCAGG","transcript_product_size_bp":83,"primer3_penalty":0.1488,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025403,"end":49025485,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":404,"end":486,"product_size":83,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TTCCTGGGCTTTTGGGATCC","right":"AGTAAAGCCGGCACTTCACA","transcript_product_size_bp":114,"primer3_penalty":0.1489,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_locus_accession":"NC_000077.7","target_locus_start":49025000,"target_locus_end":49026214,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":49025464,"end":49025577,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_010999.3","target_gene_id":"Or2v1","target_gene_name":"Or2v1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_010999.3","start":465,"end":578,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Or2v1","gene_name":"Or2v1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"29244530a1","gene":"Hapln4","target_transcript":"NM_177900.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CCATTGTCTTACCCTGCCGT","right":"CAGGTCCGTCATTCTGCAGT","transcript_product_size_bp":183,"primer3_penalty":0.0724,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537498,"end":70537680,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":223,"end":405,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACCATTGTCTTACCCTGCCG","right":"CAGGTCCGTCATTCTGCAGT","transcript_product_size_bp":184,"primer3_penalty":0.0724,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537497,"end":70537680,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":222,"end":405,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CCATTGTCTTACCCTGCCGT","right":"CATCCCCAGGTCCGTCATTC","transcript_product_size_bp":189,"primer3_penalty":0.2145,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537498,"end":70537686,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":223,"end":411,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"ACCATTGTCTTACCCTGCCG","right":"CATCCCCAGGTCCGTCATTC","transcript_product_size_bp":190,"primer3_penalty":0.2145,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":2,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537497,"end":70537686,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":222,"end":411,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"GGGGTGTTAGTGGCTGATCC","right":"CGCAGGGGTGTGTCATATGT","transcript_product_size_bp":99,"primer3_penalty":0.2149,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70541193,"end":70541291,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":1257,"end":1355,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GAATGACGGACCTGGGGATG","right":"CCCCTTCCAGATCCAGCTTG","transcript_product_size_bp":131,"primer3_penalty":0.2859,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537667,"end":70537797,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":392,"end":522,"product_size":131,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CAAGCTGGATCTGGAAGGGG","right":"GACGCCAAAATGCCATCCTG","transcript_product_size_bp":114,"primer3_penalty":0.2859,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537778,"end":70539554,"product_size":1777,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":503,"end":616,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGCATGGTCAAGCTGGATCT","right":"GACGCCAAAATGCCATCCTG","transcript_product_size_bp":122,"primer3_penalty":0.2859,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537770,"end":70539554,"product_size":1785,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":495,"end":616,"product_size":122,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAAGCTGGATCTGGAAGGGG","right":"CGCCAAAATGCCATCCTGTT","transcript_product_size_bp":112,"primer3_penalty":0.354,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537778,"end":70539552,"product_size":1775,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":503,"end":614,"product_size":112,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GGCATGGTCAAGCTGGATCT","right":"CGCCAAAATGCCATCCTGTT","transcript_product_size_bp":120,"primer3_penalty":0.354,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_locus_accession":"NC_000074.7","target_locus_start":70536179,"target_locus_end":70543512,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000074.7","start":70537770,"end":70539552,"product_size":1783,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177900.4","target_gene_id":"Hapln4","target_gene_name":"Hapln4","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177900.4","start":495,"end":614,"product_size":120,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Hapln4","gene_name":"Hapln4"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21312430a1","gene":"Adck1","target_transcript":"NM_001277297.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GGTCGGACAGTGGCAGTTAA","right":"GAGGCAGGTTCTTCTTCGCT","transcript_product_size_bp":157,"primer3_penalty":0.0712,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":601,"end":757,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":789,"end":945,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1396,"end":1552,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":597,"end":753,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":710,"end":866,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1386,"end":1542,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":417,"end":573,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1662,"end":1818,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":412,"end":568,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":557,"end":713,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1600,"end":1756,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1456,"end":1612,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGTCGGACAGTGGCAGTTAA","right":"AGAGGCAGGTTCTTCTTCGC","transcript_product_size_bp":158,"primer3_penalty":0.0712,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":601,"end":758,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":789,"end":946,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1396,"end":1553,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":597,"end":754,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":710,"end":867,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1386,"end":1543,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":417,"end":574,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1662,"end":1819,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":412,"end":569,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":557,"end":714,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1600,"end":1757,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1456,"end":1613,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CGGACAGTGGCAGTTAAGGT","right":"GAGGCAGGTTCTTCTTCGCT","transcript_product_size_bp":154,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":604,"end":757,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":792,"end":945,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1399,"end":1552,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":600,"end":753,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":713,"end":866,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1389,"end":1542,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":420,"end":573,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1665,"end":1818,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":415,"end":568,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":560,"end":713,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1603,"end":1756,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1459,"end":1612,"product_size":154,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CGGACAGTGGCAGTTAAGGT","right":"AGAGGCAGGTTCTTCTTCGC","transcript_product_size_bp":155,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":604,"end":758,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":792,"end":946,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1399,"end":1553,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":600,"end":754,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":713,"end":867,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1389,"end":1543,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":420,"end":574,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1665,"end":1819,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":415,"end":569,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":560,"end":714,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1603,"end":1757,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1459,"end":1613,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGTTGCATGATGGTCGGACA","right":"GAGGCAGGTTCTTCTTCGCT","transcript_product_size_bp":168,"primer3_penalty":0.0729,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":590,"end":757,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":778,"end":945,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1385,"end":1552,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":586,"end":753,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":699,"end":866,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1375,"end":1542,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":406,"end":573,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1651,"end":1818,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":401,"end":568,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":546,"end":713,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1589,"end":1756,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1445,"end":1612,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGTTGCATGATGGTCGGACA","right":"AGAGGCAGGTTCTTCTTCGC","transcript_product_size_bp":169,"primer3_penalty":0.0729,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":590,"end":758,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":778,"end":946,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1385,"end":1553,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":586,"end":754,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":699,"end":867,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1375,"end":1543,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":406,"end":574,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1651,"end":1819,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":401,"end":569,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":546,"end":714,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1589,"end":1757,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1445,"end":1613,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGTGGCAGTTAAGGTCCAGC","right":"GAGGCAGGTTCTTCTTCGCT","transcript_product_size_bp":149,"primer3_penalty":0.0737,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":609,"end":757,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":797,"end":945,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1404,"end":1552,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":605,"end":753,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":718,"end":866,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1394,"end":1542,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":425,"end":573,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1670,"end":1818,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":420,"end":568,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":565,"end":713,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1608,"end":1756,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1464,"end":1612,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AGTGGCAGTTAAGGTCCAGC","right":"AGAGGCAGGTTCTTCTTCGC","transcript_product_size_bp":150,"primer3_penalty":0.0737,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":20,"right_hit_count":25,"paired_amplicon_count":20,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":19,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":609,"end":758,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":797,"end":946,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":1404,"end":1553,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":605,"end":754,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":718,"end":867,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":1394,"end":1543,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":425,"end":574,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":1670,"end":1819,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":420,"end":569,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":565,"end":714,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246933.2","start":1608,"end":1757,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_030246934.2","start":1464,"end":1613,"product_size":150,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ATTCCTTCCTTCACGCTGGG","right":"TCCAATGGCAGAGCATGAGG","transcript_product_size_bp":103,"primer3_penalty":0.1419,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":88428003,"end":88428105,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":22,"right_hit_count":22,"paired_amplicon_count":22,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":21,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":1712,"end":1814,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":1900,"end":2002,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":2507,"end":2609,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361599.1","start":1349,"end":1451,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":1708,"end":1810,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361601.1","start":1407,"end":1509,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":1821,"end":1923,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":2497,"end":2599,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":1528,"end":1630,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":2773,"end":2875,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":1523,"end":1625,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":1668,"end":1770,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTTCGAGTGAGGGCCTTGAG","right":"CCCAGCGTGAAGGAAGGAAT","transcript_product_size_bp":141,"primer3_penalty":0.1435,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_locus_accession":"NC_000078.7","target_locus_start":88327284,"target_locus_end":88428496,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000078.7","start":88427882,"end":88428022,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001277297.1","target_gene_id":"Adck1","target_gene_name":"Adck1","left_hit_count":24,"right_hit_count":22,"paired_amplicon_count":22,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":21,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001277297.1","start":1591,"end":1731,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001277296.1","start":1779,"end":1919,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361598.1","start":2386,"end":2526,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361599.1","start":1228,"end":1368,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361600.1","start":1587,"end":1727,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_001361601.1","start":1286,"end":1426,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"NM_028105.4","start":1700,"end":1840,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516245.5","start":2376,"end":2516,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516246.4","start":1407,"end":1547,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516248.5","start":2652,"end":2792,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516251.4","start":1402,"end":1542,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"},{"transcript_accession":"XM_006516252.5","start":1547,"end":1687,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Adck1","gene_name":"Adck1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26335267a1","gene":"Nkrf","target_transcript":"NM_029891.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GAAATGAAATCCAGGGCCGC","right":"CCAGCCCATCTTTCTCAGCA","transcript_product_size_bp":95,"primer3_penalty":0.1378,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152589,"end":36152683,"product_size":95,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2344,"end":2438,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACAGGTCTCCGCTGGAAATG","right":"GCGGCCCTGGATTTCATTTC","transcript_product_size_bp":198,"primer3_penalty":0.139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152664,"end":36152861,"product_size":198,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2166,"end":2363,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCTGAGAAAGATGGGCTGG","right":"CTAGACCAAGGCCTTCTCGC","transcript_product_size_bp":108,"primer3_penalty":0.2139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152501,"end":36152608,"product_size":108,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2419,"end":2526,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAAATGAAATCCAGGGCCGC","right":"TTTCCCTAAACCACCACCCG","transcript_product_size_bp":116,"primer3_penalty":0.2139,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152568,"end":36152683,"product_size":116,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2344,"end":2459,"product_size":116,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCACGGAGCTGACCAATGAT","right":"GCCCACCCCATGAGACTTAC","transcript_product_size_bp":86,"primer3_penalty":0.2147,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152322,"end":36152407,"product_size":86,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2620,"end":2705,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTAAGTCTCATGGGGTGGGC","right":"GGCCCTCCTGTTTGAGTTGA","transcript_product_size_bp":93,"primer3_penalty":0.2177,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152249,"end":36152341,"product_size":93,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2778,"product_size":93,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCACGGAGCTGACCAATGAT","right":"GGCCCTCCTGTTTGAGTTGA","transcript_product_size_bp":159,"primer3_penalty":0.218,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152249,"end":36152407,"product_size":159,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2620,"end":2778,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CGGGTGGTGGTTTAGGGAAA","right":"ATCATTGGTCAGCTCCGTGG","transcript_product_size_bp":200,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152388,"end":36152587,"product_size":200,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2440,"end":2639,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GTAAGTCTCATGGGGTGGGC","right":"CTTGGCCCTCCTGTTTGAGT","transcript_product_size_bp":96,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152246,"end":36152341,"product_size":96,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2781,"product_size":96,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GTAAGTCTCATGGGGTGGGC","right":"ACTTGGCCCTCCTGTTTGAG","transcript_product_size_bp":97,"primer3_penalty":0.2184,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_locus_accession":"NC_000086.8","target_locus_start":36149644,"target_locus_end":36167118,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000086.8","start":36152245,"end":36152341,"product_size":97,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_029891.3","target_gene_id":"Nkrf","target_gene_name":"Nkrf","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_029891.3","start":2686,"end":2782,"product_size":97,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Nkrf","gene_name":"Nkrf"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31980912a1","gene":"Paf1","target_transcript":"NM_019458.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGGAGTAGTCTGCCGAGTCA","right":"TGGTCGAAGGGGTAGGTGAT","transcript_product_size_bp":84,"primer3_penalty":0.0799,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28094471,"end":28094554,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":293,"end":376,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2234,"end":2317,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCACTTCATCCCCTTTCCCC","right":"CTGTGGCCATCTTCCCTCTG","transcript_product_size_bp":188,"primer3_penalty":0.1404,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":8,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28092492,"end":28092679,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":72,"end":259,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2013,"end":2200,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CCCACTTCATCCCCTTTCCC","right":"CTGTGGCCATCTTCCCTCTG","transcript_product_size_bp":189,"primer3_penalty":0.1404,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28092491,"end":28092679,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":71,"end":259,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2012,"end":2200,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCAATGCCTTGCTTGTCGTC","right":"CCTCGCTGCCTTCTTTCTCA","transcript_product_size_bp":198,"primer3_penalty":0.1465,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":12,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28097920,"end":28098298,"product_size":379,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":1246,"end":1443,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":3187,"end":3384,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CAGAGGGAAGATGGCCACAG","right":"TGGTCGAAGGGGTAGGTGAT","transcript_product_size_bp":137,"primer3_penalty":0.1494,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28092660,"end":28094554,"product_size":1895,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":240,"end":376,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2181,"end":2317,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGGAGTAGTCTGCCGAGTCA","right":"TTCTGGTCGAAGGGGTAGGT","transcript_product_size_bp":87,"primer3_penalty":0.1535,"exon_span":{"spans_junction":false,"left_exon":1,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28094471,"end":28094557,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":293,"end":379,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2234,"end":2320,"product_size":87,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ATCCGGCGTCTCTCAAAGTC","right":"TGACTCGGCAGACTACTCCA","transcript_product_size_bp":187,"primer3_penalty":0.2114,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28092546,"end":28094490,"product_size":1945,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":126,"end":312,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2067,"end":2253,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GATCCGGCGTCTCTCAAAGT","right":"TGACTCGGCAGACTACTCCA","transcript_product_size_bp":188,"primer3_penalty":0.2114,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":1,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28092545,"end":28094490,"product_size":1946,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":125,"end":312,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":2066,"end":2253,"product_size":188,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCAATGCCTTGCTTGTCGTC","right":"CACTGCTGCTACCCTTCTCC","transcript_product_size_bp":180,"primer3_penalty":0.2175,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":12,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28097920,"end":28098280,"product_size":361,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":1246,"end":1425,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":3187,"end":3366,"product_size":180,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GGAGAAGGGTAGCAGCAGTG","right":"TCTGTCACCCTCTTCTCGGT","transcript_product_size_bp":82,"primer3_penalty":0.2206,"exon_span":{"spans_junction":false,"left_exon":12,"right_exon":12,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_locus_accession":"NC_000073.7","target_locus_start":28092421,"target_locus_end":28098808,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000073.7","start":28098261,"end":28098342,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_019458.3","target_gene_id":"Paf1","target_gene_name":"Paf1","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_019458.3","start":1406,"end":1487,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Paf1","gene_name":"Paf1"},{"transcript_accession":"XM_036153258.1","start":3347,"end":3428,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Paf1","gene_name":"Paf1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21450281a1","gene":"Slc35a3","target_transcript":"NM_001362374.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GATGCAGCCACTTACCAGGT","right":"GGAGAGCCACTGGTACACAC","transcript_product_size_bp":105,"primer3_penalty":0.0723,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480747,"end":116480937,"product_size":191,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":678,"end":782,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":538,"end":642,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":444,"end":548,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":507,"end":611,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":507,"end":611,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":444,"end":548,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":448,"end":552,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":305,"end":409,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":514,"end":618,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":510,"end":614,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":510,"end":614,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":538,"end":642,"product_size":105,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CCCATGGAAACCCTGAAGCT","right":"ACCTGGTAAGTGGCTGCATC","transcript_product_size_bp":104,"primer3_penalty":0.0753,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480918,"end":116481021,"product_size":104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":594,"end":697,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":454,"end":557,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":360,"end":463,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":423,"end":526,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":423,"end":526,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":360,"end":463,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":364,"end":467,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":221,"end":324,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":430,"end":533,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":426,"end":529,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":426,"end":529,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":454,"end":557,"product_size":104,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"AGCCCATGGAAACCCTGAAG","right":"ACCTGGTAAGTGGCTGCATC","transcript_product_size_bp":106,"primer3_penalty":0.0753,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480918,"end":116481023,"product_size":106,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":14,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":592,"end":697,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":452,"end":557,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":358,"end":463,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":421,"end":526,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":421,"end":526,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":358,"end":463,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":362,"end":467,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":219,"end":324,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":428,"end":533,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":424,"end":529,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":424,"end":529,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":452,"end":557,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CCCATGGAAACCCTGAAGCT","right":"GGAGAGCCACTGGTACACAC","transcript_product_size_bp":189,"primer3_penalty":0.0774,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480747,"end":116481021,"product_size":275,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":594,"end":782,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":454,"end":642,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":360,"end":548,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":423,"end":611,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":423,"end":611,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":360,"end":548,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":364,"end":552,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":221,"end":409,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":430,"end":618,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":426,"end":614,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":426,"end":614,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":454,"end":642,"product_size":189,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AGCCCATGGAAACCCTGAAG","right":"GGAGAGCCACTGGTACACAC","transcript_product_size_bp":191,"primer3_penalty":0.0774,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480747,"end":116481023,"product_size":277,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":14,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":592,"end":782,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":452,"end":642,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":358,"end":548,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":421,"end":611,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":421,"end":611,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":358,"end":548,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":362,"end":552,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":219,"end":409,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":428,"end":618,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":424,"end":614,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":424,"end":614,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":452,"end":642,"product_size":191,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GTGTGTACCAGTGGCTCTCC","right":"ACTCCAGCAAAGCCACTTGA","transcript_product_size_bp":166,"primer3_penalty":0.2207,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":14,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":763,"end":928,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":623,"end":788,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":529,"end":694,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":592,"end":757,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":592,"end":757,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":529,"end":694,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":533,"end":698,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":390,"end":555,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":599,"end":764,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":595,"end":760,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":595,"end":760,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":623,"end":788,"product_size":166,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGGGGCCTCGTTATCTGTCT","right":"TAGCGAGCTTCAGGGTTTCC","transcript_product_size_bp":168,"primer3_penalty":0.2814,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":14,"right_hit_count":13,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":451,"end":618,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":283,"end":447,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":283,"end":447,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":311,"end":478,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":217,"end":384,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":217,"end":384,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":283,"end":450,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":283,"end":450,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":311,"end":478,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":217,"end":388,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":283,"end":454,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"XM_036163085.1","start":190,"end":361,"product_size":172,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GGAAACCCTGAAGCTCGCTA","right":"ACCTGGTAAGTGGCTGCATC","transcript_product_size_bp":99,"primer3_penalty":0.2845,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480918,"end":116481016,"product_size":99,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":599,"end":697,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":459,"end":557,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":365,"end":463,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":428,"end":526,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":428,"end":526,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":365,"end":463,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":369,"end":467,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":226,"end":324,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":435,"end":533,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":431,"end":529,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":431,"end":529,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":459,"end":557,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GGAAACCCTGAAGCTCGCTA","right":"GGAGAGCCACTGGTACACAC","transcript_product_size_bp":184,"primer3_penalty":0.2866,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480747,"end":116481016,"product_size":270,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":599,"end":782,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":459,"end":642,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":365,"end":548,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":428,"end":611,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":428,"end":611,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":365,"end":548,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":369,"end":552,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":226,"end":409,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":435,"end":618,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":431,"end":614,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":431,"end":614,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":459,"end":642,"product_size":184,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"AGATGCAGCCACTTACCAGG","right":"GGAGAGCCACTGGTACACAC","transcript_product_size_bp":106,"primer3_penalty":0.2914,"exon_span":{"spans_junction":true,"left_exon":2,"right_exon":3,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_locus_accession":"NC_000069.7","target_locus_start":116463118,"target_locus_end":116505898,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000069.7","start":116480747,"end":116480938,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001362374.2","target_gene_id":"Slc35a3","target_gene_name":"Slc35a3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001362374.2","start":677,"end":782,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404697.1","start":537,"end":642,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404698.1","start":443,"end":548,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404699.1","start":506,"end":611,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404700.1","start":506,"end":611,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404701.1","start":443,"end":548,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404702.1","start":447,"end":552,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404703.1","start":304,"end":409,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_001404704.1","start":513,"end":618,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NM_144902.5","start":509,"end":614,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175853.1","start":509,"end":614,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"},{"transcript_accession":"NR_175854.1","start":537,"end":642,"product_size":106,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Slc35a3","gene_name":"Slc35a3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21313374a1","gene":"Uba5","target_transcript":"NM_025692.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"TGGCATTGAAACGGATGGGA","right":"ATTCATGTTGGCCAGCTCCA","transcript_product_size_bp":173,"primer3_penalty":0.0789,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":3,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103934138,"end":103937630,"product_size":3493,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":339,"end":511,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":786,"end":958,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":354,"end":585,"product_size":232,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":353,"end":584,"product_size":232,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CAGTGGCTGGCTTCTGTCTT","right":"ACTAAGGCGGCGCTAAGTTT","transcript_product_size_bp":90,"primer3_penalty":0.2873,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924711,"end":103924800,"product_size":90,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1389,"end":1478,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1463,"end":1552,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1914,"end":2003,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1540,"end":1629,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"CAGTGGCTGGCTTCTGTCTT","right":"AACTAAGGCGGCGCTAAGTT","transcript_product_size_bp":91,"primer3_penalty":0.2873,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924710,"end":103924800,"product_size":91,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1389,"end":1479,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1463,"end":1553,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1914,"end":2004,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1540,"end":1630,"product_size":91,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"AACTTAGCGCCGCCTTAGTT","right":"ACCCCTTCTCTTTCTGTCGC","transcript_product_size_bp":125,"primer3_penalty":0.3587,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924605,"end":103924729,"product_size":125,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1460,"end":1584,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1534,"end":1658,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1985,"end":2109,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1611,"end":1735,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AAACTTAGCGCCGCCTTAGT","right":"ACCCCTTCTCTTTCTGTCGC","transcript_product_size_bp":126,"primer3_penalty":0.3587,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924605,"end":103924730,"product_size":126,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1459,"end":1584,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1533,"end":1658,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1984,"end":2109,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1610,"end":1735,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCCAATCCCTACAGCCGTCT","right":"ATTCATGTTGGCCAGCTCCA","transcript_product_size_bp":195,"primer3_penalty":0.3654,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":3,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":317,"end":511,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":764,"end":958,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":332,"end":585,"product_size":254,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":331,"end":584,"product_size":254,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TGGCATTGAAACGGATGGGA","right":"CGATTCATGTTGGCCAGCTC","transcript_product_size_bp":175,"primer3_penalty":0.4186,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":3,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103934136,"end":103937630,"product_size":3495,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":339,"end":513,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":786,"end":960,"product_size":175,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":354,"end":587,"product_size":234,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":353,"end":586,"product_size":234,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AACTTAGCGCCGCCTTAGTT","right":"GGAAGCAGGAGGAAACCACT","transcript_product_size_bp":81,"primer3_penalty":0.4388,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":5,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924649,"end":103924729,"product_size":81,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1460,"end":1540,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1534,"end":1614,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1985,"end":2065,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1611,"end":1691,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AAACTTAGCGCCGCCTTAGT","right":"GGAAGCAGGAGGAAACCACT","transcript_product_size_bp":82,"primer3_penalty":0.4388,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000075.7","start":103924649,"end":103924730,"product_size":82,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":5,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":1459,"end":1540,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":1533,"end":1614,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":1984,"end":2065,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":1610,"end":1691,"product_size":82,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GAGCTGGCCAACATGAATCG","right":"TTCCTCAGGGTGTGTTCTGC","transcript_product_size_bp":86,"primer3_penalty":0.4883,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_locus_accession":"NC_000075.7","target_locus_start":103923787,"target_locus_end":103940320,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025692.3","target_gene_id":"Uba5","target_gene_name":"Uba5","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025692.3","start":494,"end":579,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XM_006511791.4","start":568,"end":653,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_001778969.3","start":941,"end":1026,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"},{"transcript_accession":"XR_004935516.1","start":567,"end":652,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Uba5","gene_name":"Uba5"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21313592a1","gene":"Tomm7","target_transcript":"NM_025394.3","candidate_pairs_screened":10,"combined_pass_pairs":8,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"TGGGATTTACAAGGGGTGCA","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":100,"primer3_penalty":1.0298,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":173,"end":272,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGGATTTACAAGGGGTGCAGA","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":99,"primer3_penalty":1.2546,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24044779,"end":24046758,"product_size":1980,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":174,"end":272,"product_size":99,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGGGCTTTATTCCTCTCGTG","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":128,"primer3_penalty":1.4586,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24044779,"end":24048999,"product_size":4221,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":145,"end":272,"product_size":128,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGGGATTTACAAGGGGTGCAG","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":100,"primer3_penalty":1.5215,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":173,"end":272,"product_size":100,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGGGATTTACAAGGGGTGCA","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":101,"primer3_penalty":1.5215,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":172,"end":272,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TTGTTGGGCTCCTCGTTCC","right":"CATTCCAGGATCTGCACCCC","transcript_product_size_bp":168,"primer3_penalty":1.5394,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24046728,"end":24049107,"product_size":2380,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":37,"end":204,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCGCCATGGTGAAGCTGAG","right":"CATTCCAGGATCTGCACCCC","transcript_product_size_bp":137,"primer3_penalty":1.5458,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":7,"right_hit_count":4,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24046728,"end":24049076,"product_size":2349,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000068.8","start":34854786,"end":34854921,"product_size":136,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":165554254,"end":165554393,"product_size":140,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":68,"end":204,"product_size":137,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":8,"left":"ACCTGGGATTTACAAGGGGTG","right":"TCGCGTCCAAATCCAGATGT","transcript_product_size_bp":103,"primer3_penalty":1.6069,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":170,"end":272,"product_size":103,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CCATGGTGAAGCTGAGCAAAG","right":"CATTCCAGGATCTGCACCCC","transcript_product_size_bp":134,"primer3_penalty":1.6686,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":3,"target_amplicon_count":1,"off_target_amplicon_count":2,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24046728,"end":24049073,"product_size":2346,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000068.8","start":34854786,"end":34854918,"product_size":133,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000086.8","start":165554254,"end":165554390,"product_size":137,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":71,"end":204,"product_size":134,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"GGTTGTTGGGCTCCTCGTT","right":"CATTCCAGGATCTGCACCCC","transcript_product_size_bp":170,"primer3_penalty":1.6951,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_locus_accession":"NC_000071.7","target_locus_start":24043942,"target_locus_end":24049143,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000071.7","start":24046728,"end":24049109,"product_size":2382,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_025394.3","target_gene_id":"Tomm7","target_gene_name":"Tomm7","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_025394.3","start":35,"end":204,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Tomm7","gene_name":"Tomm7"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"14211280a1","gene":"Acaca","target_transcript":"NM_133360.3","candidate_pairs_screened":10,"combined_pass_pairs":7,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GGTCTTCGAGTGGATTGGCA","right":"ATCCCTTTCCCTCCTCCTCC","transcript_product_size_bp":173,"primer3_penalty":0.066,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":33,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119745,"end":84122541,"product_size":2797,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":956,"end":1128,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1326,"end":1498,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1093,"end":1265,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1060,"end":1232,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1044,"end":1216,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1374,"end":1546,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1309,"end":1481,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1374,"end":1546,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1072,"end":1244,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1144,"end":1316,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1334,"end":1506,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1396,"end":1568,"product_size":173,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"TGGTCTTCGAGTGGATTGGC","right":"ATCCCTTTCCCTCCTCCTCC","transcript_product_size_bp":174,"primer3_penalty":0.066,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":32,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119744,"end":84122541,"product_size":2798,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":955,"end":1128,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1325,"end":1498,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1092,"end":1265,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1059,"end":1232,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1043,"end":1216,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1373,"end":1546,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1308,"end":1481,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1373,"end":1546,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1071,"end":1244,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1143,"end":1316,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1333,"end":1506,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1395,"end":1568,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGCTGCTGGAGGACTTCATG","right":"ACCCCGAATAGACAGCTCCT","transcript_product_size_bp":124,"primer3_penalty":0.0677,"exon_span":{"spans_junction":true,"left_exon":13,"right_exon":14,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84136600,"end":84140279,"product_size":3680,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10202850,"end":10202973,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":1798,"end":1921,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2168,"end":2291,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1935,"end":2058,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1902,"end":2025,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1886,"end":2009,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2216,"end":2339,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2151,"end":2274,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2216,"end":2339,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1914,"end":2037,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1986,"end":2109,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2176,"end":2299,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2238,"end":2361,"product_size":124,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":4,"left":"AGGAGCTGTCTATTCGGGGT","right":"CCAGCCAGTGTCGATTCTGT","transcript_product_size_bp":101,"primer3_penalty":0.069,"exon_span":{"spans_junction":false,"left_exon":14,"right_exon":14,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84140260,"end":84140360,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10202954,"end":10203054,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":15,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":1902,"end":2002,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2272,"end":2372,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":2039,"end":2139,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":2006,"end":2106,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1990,"end":2090,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2320,"end":2420,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2255,"end":2355,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2320,"end":2420,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":2018,"end":2118,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":2090,"end":2190,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2280,"end":2380,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2342,"end":2442,"product_size":101,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":5,"left":"TGCCTCTGAGAACCCGAAAC","right":"GCCAATCCACTCGAAGACCA","transcript_product_size_bp":176,"primer3_penalty":0.0713,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115293,"end":84119763,"product_size":4471,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":799,"end":974,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1169,"end":1344,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":936,"end":1111,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":903,"end":1078,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":887,"end":1062,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1217,"end":1392,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1152,"end":1327,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1217,"end":1392,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":915,"end":1090,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":987,"end":1162,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1177,"end":1352,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1239,"end":1414,"product_size":176,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TGCCTCTGAGAACCCGAAAC","right":"TGCCAATCCACTCGAAGACC","transcript_product_size_bp":177,"primer3_penalty":0.0713,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115293,"end":84119764,"product_size":4472,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":799,"end":975,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1169,"end":1345,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":936,"end":1112,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":903,"end":1079,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":887,"end":1063,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1217,"end":1393,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1152,"end":1328,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1217,"end":1393,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":915,"end":1091,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":987,"end":1163,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1177,"end":1353,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1239,"end":1415,"product_size":177,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CCGAAACTCCCAGAACTGCT","right":"GCCAATCCACTCGAAGACCA","transcript_product_size_bp":163,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115306,"end":84119763,"product_size":4458,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":812,"end":974,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1182,"end":1344,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":949,"end":1111,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":916,"end":1078,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":900,"end":1062,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1230,"end":1392,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1165,"end":1327,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1230,"end":1392,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":928,"end":1090,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1000,"end":1162,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1190,"end":1352,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1252,"end":1414,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CCGAAACTCCCAGAACTGCT","right":"TGCCAATCCACTCGAAGACC","transcript_product_size_bp":164,"primer3_penalty":0.0718,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":8,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84115306,"end":84119764,"product_size":4459,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":14,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":812,"end":975,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1182,"end":1345,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":949,"end":1112,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":916,"end":1079,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":900,"end":1063,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1230,"end":1393,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1165,"end":1328,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1230,"end":1393,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":928,"end":1091,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1000,"end":1163,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1190,"end":1353,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1252,"end":1415,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GACATGTGTTTGCTGGCCAG","right":"ATCACACAGCCAGGGTCAAG","transcript_product_size_bp":133,"primer3_penalty":0.0762,"exon_span":{"spans_junction":true,"left_exon":18,"right_exon":19,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":4,"paired_amplicon_count":2,"target_amplicon_count":1,"off_target_amplicon_count":1,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84150262,"end":84151544,"product_size":1283,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000078.7","start":10203530,"end":10203662,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":15,"right_hit_count":16,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":2478,"end":2610,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":2848,"end":2980,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":2615,"end":2747,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":2582,"end":2714,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":2566,"end":2698,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":2896,"end":3028,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":2831,"end":2963,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":2896,"end":3028,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":2594,"end":2726,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":2666,"end":2798,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":2856,"end":2988,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":2918,"end":3050,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":false},{"rank":10,"left":"GGTCTTCGAGTGGATTGGCA","right":"TTTCCCTCCTCCTCCCTCTG","transcript_product_size_bp":168,"primer3_penalty":0.0797,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":9,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_locus_accession":"NC_000077.7","target_locus_start":84070657,"target_locus_end":84292477,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":20,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":84119745,"end":84122536,"product_size":2792,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_133360.3","target_gene_id":"Acaca","target_gene_name":"Acaca","left_hit_count":14,"right_hit_count":16,"paired_amplicon_count":14,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":13,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_133360.3","start":956,"end":1123,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531953.3","start":1326,"end":1493,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531954.3","start":1093,"end":1260,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531955.3","start":1060,"end":1227,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_006531956.3","start":1044,"end":1211,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248666.3","start":1374,"end":1541,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_011248667.2","start":1309,"end":1476,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245461.2","start":1374,"end":1541,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245462.2","start":1072,"end":1239,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_030245463.1","start":1144,"end":1311,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156215.1","start":1334,"end":1501,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"},{"transcript_accession":"XM_036156216.1","start":1396,"end":1563,"product_size":168,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Acaca","gene_name":"Acaca"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"23943838a1","gene":"Slc25a1","target_transcript":"NM_153150.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GGAGGCACACAAATACCGGA","right":"GGTGCCCTTGTAGAATGCCT","transcript_product_size_bp":85,"primer3_penalty":0.0699,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":893,"end":977,"product_size":85,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ACGCAACTGGTACCAAGGAG","right":"TCCGGTATTTGTGTGCCTCC","transcript_product_size_bp":158,"primer3_penalty":0.0707,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":7,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":755,"end":912,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGAGGCACACAAATACCGGA","right":"GGAACGGTGCCCTTGTAGAA","transcript_product_size_bp":90,"primer3_penalty":0.0716,"exon_span":{"spans_junction":false,"left_exon":7,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17743771,"end":17743962,"product_size":192,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":15,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":893,"end":982,"product_size":90,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCGAGTTCCTCAGCAACCAC","right":"CCATAGGGCACACGACTACC","transcript_product_size_bp":117,"primer3_penalty":0.1378,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17745029,"end":17745145,"product_size":117,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":454,"end":570,"product_size":117,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTCCCTACGCAACTGGTACC","right":"TCCGGTATTTGTGTGCCTCC","transcript_product_size_bp":164,"primer3_penalty":0.2109,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":7,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17743943,"end":17744321,"product_size":379,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":749,"end":912,"product_size":164,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CCTCCCTACGCAACTGGTAC","right":"TCCGGTATTTGTGTGCCTCC","transcript_product_size_bp":165,"primer3_penalty":0.2109,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":7,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17743943,"end":17744322,"product_size":380,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":748,"end":912,"product_size":165,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"TCGAGTTCCTCAGCAACCAC","right":"GTCTCCATAGGGCACACGAC","transcript_product_size_bp":121,"primer3_penalty":0.2126,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":3,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17745025,"end":17745145,"product_size":121,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":454,"end":574,"product_size":121,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TCGAGTTCCTCAGCAACCAC","right":"CTCGGTACTTGGGGTTGGAG","transcript_product_size_bp":174,"primer3_penalty":0.2829,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000082.7","start":17744559,"end":17745145,"product_size":587,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":454,"end":627,"product_size":174,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"TACCAAGGCCTTACTGCGAC","right":"CTCCTTGGTACCAGTTGCGT","transcript_product_size_bp":94,"primer3_penalty":0.2832,"exon_span":{"spans_junction":true,"left_exon":5,"right_exon":6,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":681,"end":774,"product_size":94,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CTCCAACCCCAAGTACCGAG","right":"CTCCTTGGTACCAGTTGCGT","transcript_product_size_bp":167,"primer3_penalty":0.2843,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":6,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_locus_accession":"NC_000082.7","target_locus_start":17743075,"target_locus_end":17746083,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_153150.2","target_gene_id":"Slc25a1","target_gene_name":"Slc25a1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_153150.2","start":608,"end":774,"product_size":167,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc25a1","gene_name":"Slc25a1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"7656912a1","gene":"Atp8a2","target_transcript":"NM_015803.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GTGTCCCCTCTGCAGAAGTC","right":"GTTGGTAGCCTGCATTCCCT","transcript_product_size_bp":159,"primer3_penalty":0.0709,"exon_span":{"spans_junction":false,"left_exon":23,"right_exon":23,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60097603,"end":60097761,"product_size":159,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2498,"end":2656,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTGGTGCCCTTGGTCATCAT","right":"TTCACGATGTCTCCCACTGC","transcript_product_size_bp":170,"primer3_penalty":0.0711,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":4,"junction_count":3},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":473,"end":642,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GTGTCCCCTCTGCAGAAGTC","right":"CTACGTGGGCTGTCTGGATC","transcript_product_size_bp":118,"primer3_penalty":0.1401,"exon_span":{"spans_junction":false,"left_exon":23,"right_exon":23,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60097644,"end":60097761,"product_size":118,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2498,"end":2615,"product_size":118,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"AGGGAATGCAGGCTACCAAC","right":"CACTTGGTCACCCGGTTGTA","transcript_product_size_bp":109,"primer3_penalty":0.1412,"exon_span":{"spans_junction":true,"left_exon":23,"right_exon":24,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60094524,"end":60097622,"product_size":3099,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2637,"end":2745,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"AGGGAATGCAGGCTACCAAC","right":"TACACTTGGTCACCCGGTTG","transcript_product_size_bp":111,"primer3_penalty":0.1412,"exon_span":{"spans_junction":true,"left_exon":23,"right_exon":24,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60094522,"end":60097622,"product_size":3101,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2637,"end":2747,"product_size":111,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"AGGGAATGCAGGCTACCAAC","right":"GGATACACTTGGTCACCCGG","transcript_product_size_bp":114,"primer3_penalty":0.1419,"exon_span":{"spans_junction":true,"left_exon":23,"right_exon":24,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60094519,"end":60097622,"product_size":3104,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2637,"end":2750,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GATCCAGACAGCCCACGTAG","right":"CGTGGACCAAAAGCAGCTTC","transcript_product_size_bp":119,"primer3_penalty":0.1433,"exon_span":{"spans_junction":true,"left_exon":23,"right_exon":24,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60094555,"end":60097663,"product_size":3109,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2596,"end":2714,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"GTGTCCCCTCTGCAGAAGTC","right":"GTAGCCTGCATTCCCTCGTT","transcript_product_size_bp":155,"primer3_penalty":0.1445,"exon_span":{"spans_junction":false,"left_exon":23,"right_exon":23,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60097607,"end":60097761,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":2498,"end":2652,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"ACCTGCACTTGGATGGGAAG","right":"ACTGTGTGTTCCTGAGCTGG","transcript_product_size_bp":84,"primer3_penalty":0.1462,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60271209,"end":60272655,"product_size":1447,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":870,"end":953,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ACCTGCACTTGGATGGGAAG","right":"CCACTGTGTGTTCCTGAGCT","transcript_product_size_bp":86,"primer3_penalty":0.1462,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_locus_accession":"NC_000080.7","target_locus_start":59884980,"target_locus_end":60324363,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":6,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000080.7","start":60271207,"end":60272655,"product_size":1449,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_015803.3","target_gene_id":"Atp8a2","target_gene_name":"Atp8a2","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_015803.3","start":870,"end":955,"product_size":86,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Atp8a2","gene_name":"Atp8a2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"31541870a1","gene":"Fastkd3","target_transcript":"NM_001330423.1","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CCTCACACCTGACCAATGCT","right":"CACAACTCGCACCTTGAAGC","transcript_product_size_bp":195,"primer3_penalty":0.0783,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732189,"end":68732383,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":7,"right_hit_count":12,"paired_amplicon_count":6,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":5,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":921,"end":1115,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":673,"end":867,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":673,"end":867,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":629,"end":823,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":928,"end":1122,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":629,"end":823,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTCAGCGCGAATTACCATGC","right":"GTGATGGGGAAAGGGAACGT","transcript_product_size_bp":109,"primer3_penalty":0.0796,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68730609,"end":68730717,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":281,"end":389,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":134,"end":242,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":288,"end":396,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":287,"end":395,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ACCTCCGTCCTAGAGTGTCC","right":"AGGGCGTCAACACCTTTGAA","transcript_product_size_bp":190,"primer3_penalty":0.1412,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733353,"end":68735938,"product_size":2586,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1910,"end":2099,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1662,"end":1851,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1662,"end":1851,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":419,"end":608,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1618,"end":1807,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":1071,"end":1260,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1166,"end":1355,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_017315595.2","start":869,"end":1058,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1917,"end":2106,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":1071,"end":1260,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":1027,"end":1216,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1319,"end":1508,"product_size":190,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TCCGTCCTAGAGTGTCCGTT","right":"AGGGCGTCAACACCTTTGAA","transcript_product_size_bp":187,"primer3_penalty":0.1442,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733356,"end":68735938,"product_size":2583,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":13,"paired_amplicon_count":13,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":12,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1913,"end":2099,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1665,"end":1851,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1665,"end":1851,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":422,"end":608,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1621,"end":1807,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":1074,"end":1260,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1169,"end":1355,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_017315595.2","start":872,"end":1058,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1920,"end":2106,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":1074,"end":1260,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":1030,"end":1216,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1322,"end":1508,"product_size":187,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CTGCTCCGCTTGCTTTTCTG","right":"GTGATGGGGAAAGGGAACGT","transcript_product_size_bp":157,"primer3_penalty":0.1476,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68730561,"end":68730717,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":4,"right_hit_count":4,"paired_amplicon_count":4,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":3,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":233,"end":389,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":86,"end":242,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":240,"end":396,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":239,"end":395,"product_size":157,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"CTGATTGAGCGACATCCCGT","right":"TGGGGCCCTTATAGAACGGA","transcript_product_size_bp":163,"primer3_penalty":0.21,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1784,"end":1946,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1536,"end":1698,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1536,"end":1698,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1492,"end":1654,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":945,"end":1107,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1040,"end":1202,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1791,"end":1953,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":945,"end":1107,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":901,"end":1063,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1193,"end":1355,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1492,"end":1654,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AGCTCAATTACGTGCCACCA","right":"ACGGGATGTCGCTCAATCAG","transcript_product_size_bp":139,"primer3_penalty":0.2153,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732933,"end":68733071,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":11,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1665,"end":1803,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1417,"end":1555,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1417,"end":1555,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1373,"end":1511,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":826,"end":964,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":921,"end":1059,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1672,"end":1810,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":826,"end":964,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":782,"end":920,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1074,"end":1212,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1373,"end":1511,"product_size":139,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTGATTGAGCGACATCCCGT","right":"AACGGACACTCTAGGACGGA","transcript_product_size_bp":149,"primer3_penalty":0.2164,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68733052,"end":68733375,"product_size":324,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1784,"end":1932,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1536,"end":1684,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1536,"end":1684,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1492,"end":1640,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":945,"end":1093,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1040,"end":1188,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1791,"end":1939,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":945,"end":1093,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":901,"end":1049,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1193,"end":1341,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1492,"end":1640,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"GCTCCTTCATTCATGTGCGC","right":"TGGGGCCCTTATAGAACGGA","transcript_product_size_bp":182,"primer3_penalty":0.2793,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":2,"junction_count":2},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":11,"right_hit_count":13,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":10,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1765,"end":1946,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":1517,"end":1698,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":1517,"end":1698,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":1473,"end":1654,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":926,"end":1107,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":1021,"end":1202,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1772,"end":1953,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":926,"end":1107,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":882,"end":1063,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":1174,"end":1355,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":1473,"end":1654,"product_size":182,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"ATGCCAACGTCGTCTCCAAA","right":"TTCAGGGTCTCACAACTCGC","transcript_product_size_bp":102,"primer3_penalty":0.2836,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_locus_accession":"NC_000079.7","target_locus_start":68730329,"target_locus_end":68740457,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":68732292,"end":68732393,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_001330423.1","target_gene_id":"Fastkd3","target_gene_name":"Fastkd3","left_hit_count":13,"right_hit_count":12,"paired_amplicon_count":12,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":11,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_001330423.1","start":1024,"end":1125,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NM_027123.5","start":776,"end":877,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138477.1","start":776,"end":877,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"NR_138478.1","start":179,"end":280,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517364.4","start":732,"end":833,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517366.4","start":185,"end":286,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XM_006517367.1","start":280,"end":381,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950475.1","start":1031,"end":1132,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_003950476.1","start":185,"end":286,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938066.1","start":141,"end":242,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_004938067.1","start":433,"end":534,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"},{"transcript_accession":"XR_382449.4","start":732,"end":833,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd3","gene_name":"Fastkd3"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"27369557a1","gene":"Fastkd2","target_transcript":"NM_172422.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"GAACTGCTGACTTCGGGTGA","right":"TCTCGTTTGGCTGTGATGCT","transcript_product_size_bp":145,"primer3_penalty":0.0688,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1832,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1753,"product_size":145,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GAACTGCTGACTTCGGGTGA","right":"GACCTCTGCAAGCTTCTCGT","transcript_product_size_bp":159,"primer3_penalty":0.0713,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000067.7","start":63785149,"end":63787259,"product_size":2111,"orientation":"left_plus_right_minus","left_mismatches":2,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1846,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1767,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GAACTGCTGACTTCGGGTGA","right":"CGTTTGGCTGTGATGCTGAC","transcript_product_size_bp":142,"primer3_penalty":0.1436,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":14,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1829,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1750,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"GAACTGCTGACTTCGGGTGA","right":"CTCGTTTGGCTGTGATGCTG","transcript_product_size_bp":144,"primer3_penalty":0.201,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":14,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1831,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1752,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGACCACCTCTTGTTCAGCC","right":"TTGATGCGCTCCTGAATCGT","transcript_product_size_bp":102,"primer3_penalty":0.2168,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":799,"end":900,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":825,"end":926,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GAACTGCTGACTTCGGGTGA","right":"TCGTTTGGCTGTGATGCTGA","transcript_product_size_bp":143,"primer3_penalty":0.2838,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":3,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":14,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1830,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1751,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"AACTGCTGACTTCGGGTGAC","right":"TCTCGTTTGGCTGTGATGCT","transcript_product_size_bp":144,"primer3_penalty":0.2852,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1689,"end":1832,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1610,"end":1753,"product_size":144,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"AACTGCTGACTTCGGGTGAC","right":"GACCTCTGCAAGCTTCTCGT","transcript_product_size_bp":158,"primer3_penalty":0.2877,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1689,"end":1846,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1610,"end":1767,"product_size":158,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"AGAGGCGCTTTGAGAGACAG","right":"TTGATGCGCTCCTGAATCGT","transcript_product_size_bp":193,"primer3_penalty":0.3543,"exon_span":{"spans_junction":true,"left_exon":1,"right_exon":2,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":0,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":708,"end":900,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":734,"end":926,"product_size":193,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"GAACTGCTGACTTCGGGTGA","right":"TGAGGACCTCTGCAAGCTTC","transcript_product_size_bp":163,"primer3_penalty":0.3565,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_locus_accession":"NC_000067.7","target_locus_start":63769784,"target_locus_end":63792544,"target_locus_strand":"+","left_hit_count":0,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_172422.3","target_gene_id":"Fastkd2","target_gene_name":"Fastkd2","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":2,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":1,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_172422.3","start":1688,"end":1850,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd2","gene_name":"Fastkd2"},{"transcript_accession":"XM_006496313.2","start":1609,"end":1771,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd2","gene_name":"Fastkd2"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"28436912a1","gene":"Slc22a20","target_transcript":"NM_198650.2","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"GGAAGGGGAGAGGCTGACTA","right":"AAGATGGAGTTGGAGGTGCG","transcript_product_size_bp":84,"primer3_penalty":0.068,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6030445,"end":6031682,"product_size":1238,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":906,"end":989,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GGAAGGGGAGAGGCTGACTA","right":"AGCACGTGACCCTTCTGATG","transcript_product_size_bp":125,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6030404,"end":6031682,"product_size":1279,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":906,"end":1030,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"GGAAGGGGAGAGGCTGACTA","right":"CAGCACGTGACCCTTCTGAT","transcript_product_size_bp":126,"primer3_penalty":0.069,"exon_span":{"spans_junction":true,"left_exon":4,"right_exon":5,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6030403,"end":6031682,"product_size":1280,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":906,"end":1031,"product_size":126,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGCAAGGATGGTTGGGTCT","right":"CAAGACCACCAAACAAGGCG","transcript_product_size_bp":155,"primer3_penalty":0.0694,"exon_span":{"spans_junction":true,"left_exon":0,"right_exon":1,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6035615,"end":6035851,"product_size":237,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":11,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":321,"end":475,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"CCACCAATGTCTCAGGGGTC","right":"AGACCCAACCATCCTTGCAG","transcript_product_size_bp":162,"primer3_penalty":0.0738,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6035832,"end":6035993,"product_size":162,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":179,"end":340,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TCCCACAGAGATCAGGCAGA","right":"ACATTGCGGGTCTCAGTCAG","transcript_product_size_bp":186,"primer3_penalty":0.0786,"exon_span":{"spans_junction":true,"left_exon":7,"right_exon":8,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":1356,"end":1541,"product_size":186,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"CTCACCACTGCCAACATCCT","right":"CTGGTTCAGGGGTATGGCAG","transcript_product_size_bp":89,"primer3_penalty":0.1443,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6035944,"end":6036032,"product_size":89,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":5,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":140,"end":228,"product_size":89,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTGCCATACCCCTGAACCAG","right":"AGACCCAACCATCCTTGCAG","transcript_product_size_bp":132,"primer3_penalty":0.1458,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6035832,"end":6035963,"product_size":132,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":1,"right_hit_count":4,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":209,"end":340,"product_size":132,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CAGAGGCCGTACAGTAGCAG","right":"GGATGACTCTGGAAGCCACC","transcript_product_size_bp":169,"primer3_penalty":0.2089,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6031763,"end":6033010,"product_size":1248,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":10,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":657,"end":825,"product_size":169,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CAGAGGCCGTACAGTAGCAG","right":"GGGATGACTCTGGAAGCCAC","transcript_product_size_bp":170,"primer3_penalty":0.2089,"exon_span":{"spans_junction":true,"left_exon":3,"right_exon":4,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_locus_accession":"NC_000085.7","target_locus_start":6020262,"target_locus_end":6036171,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000085.7","start":6031762,"end":6033010,"product_size":1249,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_198650.2","target_gene_id":"Slc22a20","target_gene_name":"Slc22a20","left_hit_count":11,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_198650.2","start":657,"end":826,"product_size":170,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Slc22a20","gene_name":"Slc22a20"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"28195107a1","gene":"Pik3ip1","target_transcript":"NM_178149.4","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":10,"candidates":[{"rank":1,"left":"CTGCAGTTGGGAGGAGCTAC","right":"CCTCCTCACCCTCACCCATA","transcript_product_size_bp":125,"primer3_penalty":0.1391,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":4,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":3291783,"end":3291907,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":7,"right_hit_count":2,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":1055,"end":1179,"product_size":125,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"CTGCAGTTGGGAGGAGCTAC","right":"TGATAGGACCCTACCCAGCC","transcript_product_size_bp":143,"primer3_penalty":0.2131,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":3291783,"end":3291925,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":1055,"end":1197,"product_size":143,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"TGGAGCTGGCATTATCGTGG","right":"CAGACAGGGGCAAGGTGATT","transcript_product_size_bp":113,"primer3_penalty":0.2178,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":759,"end":871,"product_size":113,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TGGAGCTGGCATTATCGTGG","right":"TGATTCGCTGCATCTCCCTC","transcript_product_size_bp":98,"primer3_penalty":0.2846,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":759,"end":856,"product_size":98,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TGGAGCTGGCATTATCGTGG","right":"ACAGGTGGGGTTTGTGAAGG","transcript_product_size_bp":133,"primer3_penalty":0.2853,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":2,"right_hit_count":2,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":759,"end":891,"product_size":133,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"GCTGGCATTATCGTGGGCTA","right":"CAGACAGGGGCAAGGTGATT","transcript_product_size_bp":109,"primer3_penalty":0.289,"exon_span":{"spans_junction":false,"left_exon":3,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":763,"end":871,"product_size":109,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"ACAAACCCCACCTGTGAGAC","right":"GTAGCTCCTCCCAACTGCAG","transcript_product_size_bp":198,"primer3_penalty":0.2907,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":3291605,"end":3291802,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":1,"right_hit_count":7,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":877,"end":1074,"product_size":198,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"CTGCAGTTGGGAGGAGCTAC","right":"ATAGGACCCTACCCAGCCTC","transcript_product_size_bp":141,"primer3_penalty":0.2993,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":3291783,"end":3291923,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":1055,"end":1195,"product_size":141,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CTGCAGTTGGGAGGAGCTAC","right":"GATAGGACCCTACCCAGCCT","transcript_product_size_bp":142,"primer3_penalty":0.2993,"exon_span":{"spans_junction":false,"left_exon":null,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":3,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000077.7","start":3291783,"end":3291924,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":7,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":1055,"end":1196,"product_size":142,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"CGGGATCAGTCAGCTTGTGA","right":"TGATTCGCTGCATCTCCCTC","transcript_product_size_bp":200,"primer3_penalty":0.3531,"exon_span":{"spans_junction":false,"left_exon":2,"right_exon":null,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"no_paired_amplicons","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_locus_accession":"NC_000077.7","target_locus_start":3280731,"target_locus_end":3292971,"target_locus_strand":"+","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":0,"target_amplicon_count":0,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[],"message":"No opposing primer hits formed a 50–5000 bp genomic product."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_178149.4","target_gene_id":"Pik3ip1","target_gene_name":"Pik3ip1","left_hit_count":4,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_178149.4","start":657,"end":856,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Pik3ip1","gene_name":"Pik3ip1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"21552958a1","gene":"Vmn1r191","target_transcript":"NM_145849.1","candidate_pairs_screened":10,"combined_pass_pairs":6,"transcript_gene_specific_pairs":6,"transcript_isoform_specific_pairs":6,"candidates":[{"rank":1,"left":"TGTCTGACCCTTGCTTACGC","right":"AGCAGAACAACATCCAGCCA","transcript_product_size_bp":81,"primer3_penalty":0.4315,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22362862,"end":22362942,"product_size":81,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":811,"end":891,"product_size":81,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"ATTCTGGTTGGCACTCTGGG","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":162,"primer3_penalty":0.4367,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":8,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363710,"product_size":162,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":1,"right_hit_count":8,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":43,"end":204,"product_size":162,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATTCTGGTTGGCACTCTGGG","right":"GGTTTCTTCCCAGTGCTCCT","transcript_product_size_bp":80,"primer3_penalty":0.4409,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363631,"end":22363710,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":43,"end":122,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"TTGGCACTCTGGGGAACATG","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":155,"primer3_penalty":0.6492,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":17,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363703,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":22805002,"end":22805156,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22286632,"end":22286786,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22910589,"end":22910743,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22990485,"end":22990639,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":17,"right_hit_count":6,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":50,"end":204,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"},{"transcript_accession":"NM_134216.1","start":50,"end":204,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r206","gene_name":"Vmn1r206"},{"transcript_accession":"NM_001013787.1","start":50,"end":204,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r209","gene_name":"Vmn1r209"},{"transcript_accession":"NM_001166709.1","start":50,"end":204,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r207","gene_name":"Vmn1r207"},{"transcript_accession":"NM_145844.1","start":50,"end":204,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r189","gene_name":"Vmn1r189"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":5,"left":"GTTGGCACTCTGGGGAACAT","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":156,"primer3_penalty":0.6493,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":14,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363704,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":22286632,"end":22286787,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22805002,"end":22805157,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22910589,"end":22910744,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22990485,"end":22990640,"product_size":156,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":14,"right_hit_count":6,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":49,"end":204,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"},{"transcript_accession":"NM_001013787.1","start":49,"end":204,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r209","gene_name":"Vmn1r209"},{"transcript_accession":"NM_001166709.1","start":49,"end":204,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r207","gene_name":"Vmn1r207"},{"transcript_accession":"NM_134216.1","start":49,"end":204,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r206","gene_name":"Vmn1r206"},{"transcript_accession":"NM_145844.1","start":49,"end":204,"product_size":156,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r189","gene_name":"Vmn1r189"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":6,"left":"CTCAGTTGGTGGAGGAGCAC","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":114,"primer3_penalty":0.7191,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":8,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363662,"product_size":114,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":2,"right_hit_count":7,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":91,"end":204,"product_size":114,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GCACTCTGGGGAACATGTCT","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":152,"primer3_penalty":0.7242,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":15,"right_hit_count":8,"paired_amplicon_count":5,"target_amplicon_count":1,"off_target_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363700,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":22805002,"end":22805153,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22286632,"end":22286783,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22910589,"end":22910740,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22990485,"end":22990636,"product_size":152,"orientation":"right_plus_left_minus","left_mismatches":1,"right_mismatches":0,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":15,"right_hit_count":7,"paired_amplicon_count":5,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":4,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":53,"end":204,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"},{"transcript_accession":"NM_134216.1","start":53,"end":204,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r206","gene_name":"Vmn1r206"},{"transcript_accession":"NM_001013787.1","start":53,"end":204,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r209","gene_name":"Vmn1r209"},{"transcript_accession":"NM_001166709.1","start":53,"end":204,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r207","gene_name":"Vmn1r207"},{"transcript_accession":"NM_145844.1","start":53,"end":204,"product_size":152,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r189","gene_name":"Vmn1r189"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":8,"left":"AGTGTTTCAGGGTGCCATGG","right":"GGTAGAGGACTTGCTGGTGG","transcript_product_size_bp":80,"primer3_penalty":0.7909,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":false,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":28,"right_hit_count":22,"paired_amplicon_count":17,"target_amplicon_count":1,"off_target_amplicon_count":16,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363110,"end":22363189,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true},{"accession":"NC_000079.7","start":22626137,"end":22626216,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"is_target":false},{"accession":"NC_000079.7","start":22272611,"end":22272690,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22286193,"end":22286272,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22477935,"end":22478014,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22675957,"end":22676036,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22708814,"end":22708893,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22812571,"end":22812650,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":22921490,"end":22921569,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":1,"is_target":false},{"accession":"NC_000079.7","start":23219200,"end":23219279,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"is_target":false}],"message":"The paired-genome screen found additional or ambiguous amplifiable products."},"transcriptome_pair_screen":{"checked":true,"gene_specific":false,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":21,"right_hit_count":14,"paired_amplicon_count":11,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":10,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"},{"transcript_accession":"NM_134211.2","start":696,"end":775,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"other_gene","gene_id":"Vmn1r-ps103","gene_name":"Vmn1r-ps103"},{"transcript_accession":"NM_001167541.1","start":573,"end":652,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r196","gene_name":"Vmn1r196"},{"transcript_accession":"NM_134214.1","start":693,"end":772,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r214","gene_name":"Vmn1r214"},{"transcript_accession":"NM_134236.1","start":594,"end":673,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r203","gene_name":"Vmn1r203"},{"transcript_accession":"NM_134237.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r220","gene_name":"Vmn1r220"},{"transcript_accession":"NM_134238.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r219","gene_name":"Vmn1r219"},{"transcript_accession":"NM_134239.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r217","gene_name":"Vmn1r217"},{"transcript_accession":"NM_145844.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r189","gene_name":"Vmn1r189"},{"transcript_accession":"NM_145850.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r188","gene_name":"Vmn1r188"},{"transcript_accession":"NM_001013787.1","start":564,"end":643,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":1,"right_mismatches":1,"classification":"other_gene","gene_id":"Vmn1r209","gene_name":"Vmn1r209"}],"message":"The selected transcript is amplifiable, but additional cross-gene or unclassified products were found."},"combined_computational_pass":false},{"rank":9,"left":"AGGAGCACTGGGAAGAAACC","right":"AGCTGCTACTGTCTTTGGCA","transcript_product_size_bp":102,"primer3_penalty":0.8001,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":8,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22363549,"end":22363650,"product_size":102,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":1,"right_hit_count":6,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":103,"end":204,"product_size":102,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TGTCTGACCCTTGCTTACGC","right":"GCAGAACAACATCCAGCCAG","transcript_product_size_bp":80,"primer3_penalty":0.8453,"exon_span":{"spans_junction":false,"left_exon":0,"right_exon":0,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_locus_accession":"NC_000079.7","target_locus_start":22362856,"target_locus_end":22363752,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000079.7","start":22362863,"end":22362942,"product_size":80,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":true,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_145849.1","target_gene_id":"Vmn1r191","target_gene_name":"Vmn1r191","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":0,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_145849.1","start":811,"end":890,"product_size":80,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Vmn1r191","gene_name":"Vmn1r191"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]},{"primerbank_id":"26326413a1","gene":"Fastkd1","target_transcript":"NM_177244.3","candidate_pairs_screened":10,"combined_pass_pairs":10,"transcript_gene_specific_pairs":10,"transcript_isoform_specific_pairs":0,"candidates":[{"rank":1,"left":"CTCAACACTCTGCTGCTGGA","right":"TACAGCATTGGACACACGCT","transcript_product_size_bp":160,"primer3_penalty":0.0692,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69530480,"end":69532763,"product_size":2284,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1748,"end":1907,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2673,"end":2791,"product_size":119,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1312,"end":1471,"product_size":160,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":2,"left":"GCTGCTGGATAGGATCGCTT","right":"TACAGCATTGGACACACGCT","transcript_product_size_bp":149,"primer3_penalty":0.0694,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69530480,"end":69532752,"product_size":2273,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1759,"end":1907,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2684,"end":2791,"product_size":108,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1323,"end":1471,"product_size":149,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":3,"left":"ATCGCTTCAGTGGCCATTCA","right":"TACAGCATTGGACACACGCT","transcript_product_size_bp":136,"primer3_penalty":0.0699,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69530480,"end":69532739,"product_size":2260,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1772,"end":1907,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2697,"end":2791,"product_size":95,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1336,"end":1471,"product_size":136,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":4,"left":"CTGCTCTGTCCATGCTTCCA","right":"AGTACTAGCCAGGCAGACGA","transcript_product_size_bp":155,"primer3_penalty":0.0701,"exon_span":{"spans_junction":false,"left_exon":6,"right_exon":6,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69532992,"end":69533146,"product_size":155,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1365,"end":1519,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":929,"end":1083,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2290,"end":2444,"product_size":155,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":5,"left":"TATTGCCAAGGTCGCGTCTT","right":"TACAGCATTGGACACACGCT","transcript_product_size_bp":200,"primer3_penalty":0.0715,"exon_span":{"spans_junction":true,"left_exon":6,"right_exon":7,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69530480,"end":69532803,"product_size":2324,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1708,"end":1907,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2633,"end":2791,"product_size":159,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1272,"end":1471,"product_size":200,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":6,"left":"TATTGCCAAGGTCGCGTCTT","right":"TGAATGGCCACTGAAGCGAT","transcript_product_size_bp":84,"primer3_penalty":0.0719,"exon_span":{"spans_junction":false,"left_exon":6,"right_exon":6,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":1,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69532720,"end":69532803,"product_size":84,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1708,"end":1791,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1272,"end":1355,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2633,"end":2716,"product_size":84,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":7,"left":"GGTGTTATGAACGGAGCCCA","right":"TGCATGCCCAGGGATTTTCT","transcript_product_size_bp":194,"primer3_penalty":0.0774,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":11,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69521624,"end":69524321,"product_size":2698,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":4,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":2186,"end":2379,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1750,"end":1943,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":3070,"end":3263,"product_size":194,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":8,"left":"TGGTGTTATGAACGGAGCCC","right":"TGCATGCCCAGGGATTTTCT","transcript_product_size_bp":195,"primer3_penalty":0.0774,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":11,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69521624,"end":69524322,"product_size":2699,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":2185,"end":2379,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1749,"end":1943,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":3069,"end":3263,"product_size":195,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":9,"left":"CGGAGCCCAACAACAGATCT","right":"TGCATGCCCAGGGATTTTCT","transcript_product_size_bp":183,"primer3_penalty":0.0775,"exon_span":{"spans_junction":true,"left_exon":10,"right_exon":11,"junction_count":1},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":1,"right_hit_count":2,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69521624,"end":69524310,"product_size":2687,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":2197,"end":2379,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":1761,"end":1943,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":3081,"end":3263,"product_size":183,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true},{"rank":10,"left":"TCTGGTCTCTGCTCTGTCCA","right":"AGTACTAGCCAGGCAGACGA","transcript_product_size_bp":163,"primer3_penalty":0.1476,"exon_span":{"spans_junction":false,"left_exon":6,"right_exon":6,"junction_count":0},"genome_pair_screen":{"checked":true,"specific":true,"status":"validated","engine":"bowtie2_paired_amplicon","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_locus_accession":"NC_000068.8","target_locus_start":69517164,"target_locus_end":69542950,"target_locus_strand":"-","left_hit_count":2,"right_hit_count":3,"paired_amplicon_count":1,"target_amplicon_count":1,"off_target_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"accession":"NC_000068.8","start":69532992,"end":69533154,"product_size":163,"orientation":"right_plus_left_minus","left_mismatches":0,"right_mismatches":0,"is_target":true}],"message":"Exactly one target-locus product and no additional genomic product were found."},"transcriptome_pair_screen":{"checked":true,"gene_specific":true,"isoform_specific":false,"status":"validated","engine":"bowtie2_paired_transcriptome","reference_assembly":"GCF_000001635.27","target_transcript":"NM_177244.3","target_gene_id":"Fastkd1","target_gene_name":"Fastkd1","left_hit_count":3,"right_hit_count":3,"paired_amplicon_count":3,"target_transcript_amplicon_count":1,"same_gene_isoform_amplicon_count":2,"other_gene_amplicon_count":0,"unclassified_amplicon_count":0,"hit_limit_reached":false,"min_amplicon_size":50,"max_amplicon_size":5000,"top_amplicons":[{"transcript_accession":"NM_177244.3","start":1357,"end":1519,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"target_transcript","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XM_036162311.1","start":921,"end":1083,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"},{"transcript_accession":"XR_004940626.1","start":2282,"end":2444,"product_size":163,"orientation":"left_plus_right_minus","left_mismatches":0,"right_mismatches":0,"classification":"same_gene_isoform","gene_id":"Fastkd1","gene_name":"Fastkd1"}],"message":"The selected transcript is amplifiable and no other gene product was found."},"combined_computational_pass":true}]}],"case_payload_sha256":"d1c4914ec1e824ffb3e3fe2b1bbcad8d155d3aa00504153b35fc71f2b3771dfb"}